Strain Fitness in Escherichia coli ECRC100 around OKFHMN_03020

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_03010 and OKFHMN_03015 are separated by 32 nucleotidesOKFHMN_03015 and OKFHMN_03020 are separated by 33 nucleotidesOKFHMN_03020 and OKFHMN_03025 are separated by 371 nucleotidesOKFHMN_03025 and OKFHMN_03030 are separated by 68 nucleotides OKFHMN_03010: OKFHMN_03010 - DNA-binding protein, at 479,774 to 480,055 _03010 OKFHMN_03015: OKFHMN_03015 - DUF1627 domain-containing protein, at 480,088 to 480,804 _03015 OKFHMN_03020: OKFHMN_03020 - replication protein, at 480,838 to 481,500 _03020 OKFHMN_03025: OKFHMN_03025 - Putative phage replication protein, at 481,872 to 482,345 _03025 OKFHMN_03030: OKFHMN_03030 - Rha family transcriptional regulator, at 482,414 to 482,839 _03030 Position (kb) 480 481 482Strain fitness (log2 ratio) -1 0 1at 481.095 kb on - strand, within OKFHMN_03020at 481.168 kb on - strand, within OKFHMN_03020at 481.527 kb on - strandat 481.718 kb on - strandat 481.718 kb on - strandat 482.066 kb on + strand, within OKFHMN_03025at 482.067 kb on - strand, within OKFHMN_03025at 482.239 kb on + strand, within OKFHMN_03025

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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481,095 - OKFHMN_03020 0.39 +0.0
481,168 - OKFHMN_03020 0.50 -0.2
481,527 - +0.3
481,718 - -0.9
481,718 - -1.5
482,066 + OKFHMN_03025 0.41 +0.6
482,067 - OKFHMN_03025 0.41 +0.5
482,239 + OKFHMN_03025 0.77 -0.7

Or see this region's nucleotide sequence