Experiment: Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKFHMN_02995 and OKFHMN_03000 are separated by 347 nucleotides OKFHMN_03000 and OKFHMN_03005 overlap by 8 nucleotides OKFHMN_03005 and OKFHMN_03010 overlap by 4 nucleotides OKFHMN_03010 and OKFHMN_03015 are separated by 32 nucleotides
OKFHMN_02995: OKFHMN_02995 - DUF551 domain-containing protein, at 478,341 to 478,898
_02995
OKFHMN_03000: OKFHMN_03000 - Prophage protein, at 479,246 to 479,557
_03000
OKFHMN_03005: OKFHMN_03005 - hypothetical protein, at 479,550 to 479,777
_03005
OKFHMN_03010: OKFHMN_03010 - DNA-binding protein, at 479,774 to 480,055
_03010
OKFHMN_03015: OKFHMN_03015 - DUF1627 domain-containing protein, at 480,088 to 480,804
_03015
Position (kb)
479
480 Strain fitness (log2 ratio)
-2
-1
0
1 at 478.797 kb on - strand, within OKFHMN_02995 at 478.859 kb on - strand at 478.966 kb on - strand at 478.969 kb on + strand at 478.970 kb on - strand at 479.046 kb on + strand at 479.218 kb on + strand at 479.416 kb on - strand, within OKFHMN_03000 at 479.617 kb on - strand, within OKFHMN_03005 at 479.817 kb on - strand, within OKFHMN_03010
Per-strain Table
Position Strand Gene LocusTag Fraction Bas09 remove 478,797 - OKFHMN_02995 0.82 +0.5 478,859 - +0.9 478,966 - -1.8 478,969 + -1.9 478,970 - +0.2 479,046 + -0.0 479,218 + +0.9 479,416 - OKFHMN_03000 0.54 -0.3 479,617 - OKFHMN_03005 0.29 -0.2 479,817 - OKFHMN_03010 0.15 +0.3
Or see this region's nucleotide sequence