Strain Fitness in Escherichia coli ECRC100 around OKFHMN_02040

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_02020 and OKFHMN_02025 are separated by 71 nucleotidesOKFHMN_02025 and OKFHMN_02030 are separated by 275 nucleotidesOKFHMN_02030 and OKFHMN_02040 are separated by 122 nucleotidesOKFHMN_02040 and OKFHMN_02045 overlap by 14 nucleotidesOKFHMN_02045 and OKFHMN_02050 are separated by 154 nucleotides OKFHMN_02020: OKFHMN_02020 - DUF1367 domain-containing protein, at 308,336 to 308,935 _02020 OKFHMN_02025: OKFHMN_02025 - Bacteriophage cohesive ends, at 309,007 to 309,219 _02025 OKFHMN_02030: OKFHMN_02030 - Hok/gef family, at 309,495 to 309,707 _02030 OKFHMN_02040: OKFHMN_02040 - DNA-binding protein, at 309,830 to 310,951 _02040 OKFHMN_02045: OKFHMN_02045 - hypothetical protein, at 310,938 to 311,588 _02045 OKFHMN_02050: OKFHMN_02050 - hypothetical protein, at 311,743 to 311,973 _02050 Position (kb) 309 310 311Strain fitness (log2 ratio) -1 0 1at 308.894 kb on + strandat 308.983 kb on - strandat 309.356 kb on + strandat 311.205 kb on - strand, within OKFHMN_02045at 311.245 kb on - strand, within OKFHMN_02045at 311.271 kb on - strand, within OKFHMN_02045at 311.494 kb on - strand, within OKFHMN_02045at 311.494 kb on - strand, within OKFHMN_02045at 311.589 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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308,894 + +1.2
308,983 - -1.6
309,356 + +0.0
311,205 - OKFHMN_02045 0.41 -0.7
311,245 - OKFHMN_02045 0.47 +0.1
311,271 - OKFHMN_02045 0.51 -0.5
311,494 - OKFHMN_02045 0.85 -0.1
311,494 - OKFHMN_02045 0.85 +0.5
311,589 - +0.2

Or see this region's nucleotide sequence