Strain Fitness in Escherichia coli ECRC100 around OKFHMN_01120

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsad and glsB are separated by 63 nucleotidesglsB and yneG overlap by 1 nucleotidesyneG and gGDEF are separated by 138 nucleotides OKFHMN_01110: sad - succinate-semialdehyde dehydrogenase, at 109,493 to 110,881 sad OKFHMN_01115: glsB - glutaminase B, at 110,945 to 111,871 glsB OKFHMN_01120: yneG - Uncharacterized protein YneG, at 111,871 to 112,230 yneG OKFHMN_01125: gGDEF - diguanylate cyclase, at 112,369 to 113,787 gGDEF Position (kb) 111 112 113Strain fitness (log2 ratio) -2 -1 0 1at 111.355 kb on + strand, within glsBat 112.178 kb on - strand, within yneGat 112.253 kb on - strandat 112.273 kb on - strandat 112.322 kb on - strandat 112.426 kb on - strandat 112.662 kb on + strand, within gGDEFat 112.671 kb on + strand, within gGDEFat 112.755 kb on + strand, within gGDEFat 112.896 kb on + strand, within gGDEF

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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111,355 + glsB OKFHMN_01115 0.44 +0.2
112,178 - yneG OKFHMN_01120 0.85 -1.1
112,253 - -0.9
112,273 - +0.2
112,322 - +0.1
112,426 - -0.1
112,662 + gGDEF OKFHMN_01125 0.21 +0.1
112,671 + gGDEF OKFHMN_01125 0.21 -1.4
112,755 + gGDEF OKFHMN_01125 0.27 -0.8
112,896 + gGDEF OKFHMN_01125 0.37 -1.8

Or see this region's nucleotide sequence