Experiment: NL-CCM, start OD=0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX19_06046 and MPMX19_06047 are separated by 4 nucleotides MPMX19_06047 and MPMX19_06048 are separated by 336 nucleotides
MPMX19_06046: MPMX19_06046 - Sensor histidine kinase RcsC, at 203,756 to 207,691
_06046
MPMX19_06047: MPMX19_06047 - hypothetical protein, at 207,696 to 208,868
_06047
MPMX19_06048: MPMX19_06048 - hypothetical protein, at 209,205 to 211,841
_06048
Position (kb) 207
208
209 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 206.712 kb on + strand, within MPMX19_06046 at 206.712 kb on + strand, within MPMX19_06046 at 206.712 kb on + strand, within MPMX19_06046 at 206.712 kb on + strand, within MPMX19_06046 at 206.712 kb on + strand, within MPMX19_06046 at 206.712 kb on + strand, within MPMX19_06046 at 206.712 kb on + strand, within MPMX19_06046 at 206.712 kb on + strand, within MPMX19_06046 at 206.712 kb on + strand, within MPMX19_06046 at 206.712 kb on + strand, within MPMX19_06046 at 206.713 kb on - strand, within MPMX19_06046 at 206.713 kb on - strand, within MPMX19_06046 at 206.713 kb on - strand, within MPMX19_06046 at 206.713 kb on - strand, within MPMX19_06046 at 206.713 kb on - strand, within MPMX19_06046 at 206.713 kb on - strand, within MPMX19_06046 at 206.713 kb on - strand, within MPMX19_06046 at 206.713 kb on - strand, within MPMX19_06046 at 206.713 kb on - strand, within MPMX19_06046 at 206.713 kb on - strand, within MPMX19_06046 at 206.713 kb on - strand, within MPMX19_06046 at 206.713 kb on - strand, within MPMX19_06046 at 206.713 kb on - strand, within MPMX19_06046 at 206.713 kb on - strand, within MPMX19_06046 at 206.931 kb on + strand, within MPMX19_06046 at 206.931 kb on + strand, within MPMX19_06046 at 206.931 kb on + strand, within MPMX19_06046 at 206.931 kb on + strand, within MPMX19_06046 at 206.931 kb on + strand, within MPMX19_06046 at 206.931 kb on + strand, within MPMX19_06046 at 206.931 kb on + strand, within MPMX19_06046 at 206.931 kb on + strand, within MPMX19_06046 at 206.931 kb on + strand, within MPMX19_06046 at 206.931 kb on + strand, within MPMX19_06046 at 206.931 kb on + strand, within MPMX19_06046 at 206.931 kb on + strand, within MPMX19_06046 at 206.931 kb on + strand, within MPMX19_06046 at 206.931 kb on + strand, within MPMX19_06046 at 206.931 kb on + strand, within MPMX19_06046 at 206.931 kb on + strand, within MPMX19_06046 at 206.931 kb on + strand, within MPMX19_06046 at 206.931 kb on + strand, within MPMX19_06046 at 206.932 kb on - strand, within MPMX19_06046 at 206.932 kb on - strand, within MPMX19_06046 at 206.932 kb on - strand, within MPMX19_06046 at 206.932 kb on - strand, within MPMX19_06046 at 206.932 kb on - strand, within MPMX19_06046 at 206.932 kb on - strand, within MPMX19_06046 at 206.932 kb on - strand, within MPMX19_06046 at 206.932 kb on - strand, within MPMX19_06046 at 206.932 kb on - strand, within MPMX19_06046 at 206.932 kb on - strand, within MPMX19_06046 at 206.932 kb on - strand, within MPMX19_06046 at 206.932 kb on - strand, within MPMX19_06046 at 206.932 kb on - strand, within MPMX19_06046 at 206.932 kb on - strand, within MPMX19_06046 at 206.932 kb on - strand, within MPMX19_06046 at 206.932 kb on - strand, within MPMX19_06046 at 206.932 kb on - strand, within MPMX19_06046 at 206.932 kb on - strand, within MPMX19_06046 at 206.988 kb on + strand, within MPMX19_06046 at 206.988 kb on + strand, within MPMX19_06046 at 206.988 kb on + strand, within MPMX19_06046 at 206.988 kb on + strand, within MPMX19_06046 at 206.989 kb on - strand, within MPMX19_06046 at 206.989 kb on - strand, within MPMX19_06046 at 206.989 kb on - strand, within MPMX19_06046 at 206.989 kb on - strand, within MPMX19_06046 at 206.989 kb on - strand, within MPMX19_06046 at 206.989 kb on - strand, within MPMX19_06046 at 206.989 kb on - strand, within MPMX19_06046 at 206.989 kb on - strand, within MPMX19_06046 at 206.989 kb on - strand, within MPMX19_06046 at 207.171 kb on + strand, within MPMX19_06046 at 207.171 kb on + strand, within MPMX19_06046 at 207.172 kb on - strand, within MPMX19_06046 at 207.172 kb on - strand, within MPMX19_06046 at 207.172 kb on - strand, within MPMX19_06046 at 207.172 kb on - strand, within MPMX19_06046 at 207.516 kb on + strand at 207.516 kb on + strand at 207.516 kb on + strand at 207.516 kb on + strand at 207.516 kb on + strand at 207.516 kb on + strand at 207.516 kb on + strand at 207.516 kb on + strand at 207.516 kb on + strand at 207.516 kb on + strand at 207.516 kb on + strand at 207.516 kb on + strand at 207.516 kb on + strand at 207.516 kb on + strand at 207.516 kb on + strand at 207.516 kb on + strand at 207.516 kb on + strand at 207.516 kb on + strand at 207.517 kb on - strand at 207.517 kb on - strand at 207.517 kb on - strand at 207.517 kb on - strand at 207.517 kb on - strand at 207.517 kb on - strand at 207.517 kb on - strand at 207.517 kb on - strand at 207.517 kb on - strand at 207.517 kb on - strand at 207.517 kb on - strand at 207.517 kb on - strand at 207.517 kb on - strand at 207.517 kb on - strand at 207.517 kb on - strand at 207.517 kb on - strand at 207.781 kb on + strand at 207.781 kb on + strand at 207.781 kb on + strand at 207.781 kb on + strand at 207.781 kb on + strand at 207.782 kb on - strand at 207.782 kb on - strand at 207.782 kb on - strand at 207.787 kb on + strand at 207.787 kb on + strand at 207.787 kb on + strand at 207.787 kb on + strand at 207.787 kb on + strand at 207.787 kb on + strand at 207.787 kb on + strand at 207.787 kb on + strand at 207.787 kb on + strand at 207.787 kb on + strand at 207.787 kb on + strand at 207.788 kb on - strand at 207.788 kb on - strand at 207.788 kb on - strand at 207.788 kb on - strand at 207.788 kb on - strand at 207.788 kb on - strand at 207.788 kb on - strand at 207.788 kb on - strand at 207.788 kb on - strand at 207.788 kb on - strand at 207.788 kb on - strand at 207.788 kb on - strand at 207.788 kb on - strand at 207.788 kb on - strand at 207.788 kb on - strand at 207.788 kb on - strand at 207.788 kb on - strand at 207.788 kb on - strand at 207.788 kb on - strand at 207.788 kb on - strand at 208.042 kb on + strand, within MPMX19_06047 at 208.042 kb on + strand, within MPMX19_06047 at 208.042 kb on + strand, within MPMX19_06047 at 208.043 kb on - strand, within MPMX19_06047 at 208.043 kb on - strand, within MPMX19_06047 at 208.043 kb on - strand, within MPMX19_06047 at 208.043 kb on - strand, within MPMX19_06047 at 208.192 kb on + strand, within MPMX19_06047 at 208.192 kb on + strand, within MPMX19_06047 at 208.192 kb on + strand, within MPMX19_06047 at 208.192 kb on + strand, within MPMX19_06047 at 208.192 kb on + strand, within MPMX19_06047 at 208.193 kb on - strand, within MPMX19_06047 at 208.193 kb on - strand, within MPMX19_06047 at 208.193 kb on - strand, within MPMX19_06047 at 208.193 kb on - strand, within MPMX19_06047 at 208.193 kb on - strand, within MPMX19_06047 at 208.204 kb on + strand, within MPMX19_06047 at 208.204 kb on + strand, within MPMX19_06047 at 208.204 kb on + strand, within MPMX19_06047 at 208.204 kb on + strand, within MPMX19_06047 at 208.205 kb on - strand, within MPMX19_06047 at 208.205 kb on - strand, within MPMX19_06047 at 208.205 kb on - strand, within MPMX19_06047 at 208.213 kb on + strand, within MPMX19_06047 at 208.213 kb on + strand, within MPMX19_06047 at 208.213 kb on + strand, within MPMX19_06047 at 208.213 kb on + strand, within MPMX19_06047 at 208.213 kb on + strand, within MPMX19_06047 at 208.213 kb on + strand, within MPMX19_06047 at 208.213 kb on + strand, within MPMX19_06047 at 208.213 kb on + strand, within MPMX19_06047 at 208.214 kb on - strand, within MPMX19_06047 at 208.214 kb on - strand, within MPMX19_06047 at 208.214 kb on - strand, within MPMX19_06047 at 208.214 kb on - strand, within MPMX19_06047 at 208.342 kb on + strand, within MPMX19_06047 at 208.342 kb on + strand, within MPMX19_06047 at 208.343 kb on - strand, within MPMX19_06047 at 208.343 kb on - strand, within MPMX19_06047 at 208.905 kb on + strand at 208.906 kb on - strand at 208.906 kb on - strand at 208.906 kb on - strand at 208.906 kb on - strand at 208.964 kb on - strand at 209.012 kb on + strand at 209.012 kb on + strand at 209.012 kb on + strand at 209.013 kb on - strand at 209.013 kb on - strand at 209.048 kb on + strand at 209.048 kb on + strand at 209.048 kb on + strand at 209.048 kb on + strand at 209.048 kb on + strand at 209.049 kb on - strand at 209.049 kb on - strand at 209.049 kb on - strand at 209.049 kb on - strand at 209.049 kb on - strand at 209.049 kb on - strand at 209.074 kb on - strand at 209.204 kb on + strand at 209.204 kb on + strand at 209.204 kb on + strand at 209.204 kb on + strand at 209.204 kb on + strand at 209.204 kb on + strand at 209.204 kb on + strand at 209.204 kb on + strand at 209.204 kb on + strand at 209.205 kb on - strand at 209.205 kb on - strand at 209.205 kb on - strand at 209.205 kb on - strand at 209.205 kb on - strand at 209.205 kb on - strand at 209.205 kb on - strand at 209.205 kb on - strand at 209.205 kb on - strand at 209.205 kb on - strand at 209.205 kb on - strand at 209.512 kb on - strand, within MPMX19_06048 at 209.512 kb on - strand, within MPMX19_06048 at 209.512 kb on - strand, within MPMX19_06048 at 209.512 kb on - strand, within MPMX19_06048 at 209.512 kb on - strand, within MPMX19_06048 at 209.512 kb on - strand, within MPMX19_06048 at 209.718 kb on + strand, within MPMX19_06048 at 209.718 kb on + strand, within MPMX19_06048 at 209.718 kb on + strand, within MPMX19_06048 at 209.718 kb on + strand, within MPMX19_06048 at 209.719 kb on - strand, within MPMX19_06048 at 209.719 kb on - strand, within MPMX19_06048 at 209.719 kb on - strand, within MPMX19_06048 at 209.719 kb on - strand, within MPMX19_06048 at 209.719 kb on - strand, within MPMX19_06048 at 209.859 kb on + strand, within MPMX19_06048 at 209.859 kb on + strand, within MPMX19_06048 at 209.859 kb on + strand, within MPMX19_06048 at 209.859 kb on + strand, within MPMX19_06048 at 209.859 kb on + strand, within MPMX19_06048 at 209.859 kb on + strand, within MPMX19_06048 at 209.859 kb on + strand, within MPMX19_06048 at 209.859 kb on + strand, within MPMX19_06048 at 209.859 kb on + strand, within MPMX19_06048 at 209.859 kb on + strand, within MPMX19_06048 at 209.859 kb on + strand, within MPMX19_06048 at 209.859 kb on + strand, within MPMX19_06048 at 209.859 kb on + strand, within MPMX19_06048 at 209.859 kb on + strand, within MPMX19_06048 at 209.860 kb on - strand, within MPMX19_06048 at 209.860 kb on - strand, within MPMX19_06048 at 209.860 kb on - strand, within MPMX19_06048 at 209.860 kb on - strand, within MPMX19_06048 at 209.860 kb on - strand, within MPMX19_06048 at 209.860 kb on - strand, within MPMX19_06048 at 209.860 kb on - strand, within MPMX19_06048 at 209.860 kb on - strand, within MPMX19_06048 at 209.860 kb on - strand, within MPMX19_06048
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM, start OD=0.1 remove 206,712 + MPMX19_06046 0.75 +0.1 206,712 + MPMX19_06046 0.75 +1.7 206,712 + MPMX19_06046 0.75 -0.1 206,712 + MPMX19_06046 0.75 -0.7 206,712 + MPMX19_06046 0.75 -0.4 206,712 + MPMX19_06046 0.75 -0.4 206,712 + MPMX19_06046 0.75 +1.4 206,712 + MPMX19_06046 0.75 -1.1 206,712 + MPMX19_06046 0.75 -0.9 206,712 + MPMX19_06046 0.75 -0.5 206,713 - MPMX19_06046 0.75 +0.4 206,713 - MPMX19_06046 0.75 +0.5 206,713 - MPMX19_06046 0.75 +1.3 206,713 - MPMX19_06046 0.75 +0.4 206,713 - MPMX19_06046 0.75 -1.4 206,713 - MPMX19_06046 0.75 -1.6 206,713 - MPMX19_06046 0.75 -0.1 206,713 - MPMX19_06046 0.75 -0.4 206,713 - MPMX19_06046 0.75 +0.7 206,713 - MPMX19_06046 0.75 +1.4 206,713 - MPMX19_06046 0.75 +0.1 206,713 - MPMX19_06046 0.75 +0.8 206,713 - MPMX19_06046 0.75 -0.3 206,713 - MPMX19_06046 0.75 +0.8 206,931 + MPMX19_06046 0.81 -0.1 206,931 + MPMX19_06046 0.81 -0.6 206,931 + MPMX19_06046 0.81 -1.3 206,931 + MPMX19_06046 0.81 +0.1 206,931 + MPMX19_06046 0.81 +0.3 206,931 + MPMX19_06046 0.81 -0.2 206,931 + MPMX19_06046 0.81 -1.1 206,931 + MPMX19_06046 0.81 -0.4 206,931 + MPMX19_06046 0.81 +0.5 206,931 + MPMX19_06046 0.81 -0.5 206,931 + MPMX19_06046 0.81 -1.7 206,931 + MPMX19_06046 0.81 -1.0 206,931 + MPMX19_06046 0.81 -1.3 206,931 + MPMX19_06046 0.81 +1.5 206,931 + MPMX19_06046 0.81 -1.7 206,931 + MPMX19_06046 0.81 -0.2 206,931 + MPMX19_06046 0.81 -0.4 206,931 + MPMX19_06046 0.81 +0.0 206,932 - MPMX19_06046 0.81 -0.7 206,932 - MPMX19_06046 0.81 -0.9 206,932 - MPMX19_06046 0.81 +0.1 206,932 - MPMX19_06046 0.81 +0.7 206,932 - MPMX19_06046 0.81 -1.5 206,932 - MPMX19_06046 0.81 +0.5 206,932 - MPMX19_06046 0.81 -0.8 206,932 - MPMX19_06046 0.81 -1.8 206,932 - MPMX19_06046 0.81 +0.8 206,932 - MPMX19_06046 0.81 -0.6 206,932 - MPMX19_06046 0.81 +0.1 206,932 - MPMX19_06046 0.81 -1.0 206,932 - MPMX19_06046 0.81 +0.2 206,932 - MPMX19_06046 0.81 -1.2 206,932 - MPMX19_06046 0.81 -0.3 206,932 - MPMX19_06046 0.81 -0.7 206,932 - MPMX19_06046 0.81 -1.2 206,932 - MPMX19_06046 0.81 -1.4 206,988 + MPMX19_06046 0.82 +0.8 206,988 + MPMX19_06046 0.82 -1.3 206,988 + MPMX19_06046 0.82 -1.7 206,988 + MPMX19_06046 0.82 +0.1 206,989 - MPMX19_06046 0.82 +0.2 206,989 - MPMX19_06046 0.82 -0.2 206,989 - MPMX19_06046 0.82 +0.2 206,989 - MPMX19_06046 0.82 -1.3 206,989 - MPMX19_06046 0.82 -0.1 206,989 - MPMX19_06046 0.82 -0.3 206,989 - MPMX19_06046 0.82 -1.4 206,989 - MPMX19_06046 0.82 +0.7 206,989 - MPMX19_06046 0.82 -0.6 207,171 + MPMX19_06046 0.87 -0.2 207,171 + MPMX19_06046 0.87 -0.1 207,172 - MPMX19_06046 0.87 +1.7 207,172 - MPMX19_06046 0.87 -0.3 207,172 - MPMX19_06046 0.87 -0.6 207,172 - MPMX19_06046 0.87 +0.7 207,516 + -3.0 207,516 + +0.3 207,516 + +0.5 207,516 + -1.2 207,516 + +1.1 207,516 + -0.7 207,516 + +0.5 207,516 + -1.0 207,516 + -1.0 207,516 + +0.6 207,516 + +1.1 207,516 + -0.2 207,516 + -0.2 207,516 + -0.4 207,516 + +0.2 207,516 + -0.2 207,516 + -0.3 207,516 + +0.5 207,517 - -0.1 207,517 - -0.9 207,517 - -0.9 207,517 - +0.6 207,517 - +0.3 207,517 - -1.0 207,517 - +0.3 207,517 - -1.3 207,517 - -0.4 207,517 - -1.2 207,517 - -2.3 207,517 - +0.2 207,517 - +0.3 207,517 - -0.5 207,517 - -1.3 207,517 - -0.7 207,781 + +1.7 207,781 + -0.9 207,781 + -0.3 207,781 + +0.1 207,781 + -0.5 207,782 - +1.2 207,782 - +0.8 207,782 - +1.7 207,787 + +0.8 207,787 + -0.6 207,787 + -0.3 207,787 + -0.6 207,787 + +0.1 207,787 + +0.2 207,787 + -0.8 207,787 + -0.7 207,787 + +1.1 207,787 + +0.9 207,787 + +0.7 207,788 - +0.4 207,788 - -0.3 207,788 - +1.7 207,788 - +0.9 207,788 - -1.7 207,788 - -2.0 207,788 - -1.5 207,788 - -0.0 207,788 - -1.5 207,788 - -0.0 207,788 - +0.7 207,788 - -0.9 207,788 - -0.6 207,788 - -0.3 207,788 - -0.0 207,788 - -1.2 207,788 - +0.4 207,788 - +0.1 207,788 - -0.7 207,788 - +0.1 208,042 + MPMX19_06047 0.29 -0.5 208,042 + MPMX19_06047 0.29 +0.2 208,042 + MPMX19_06047 0.29 -1.2 208,043 - MPMX19_06047 0.30 +0.5 208,043 - MPMX19_06047 0.30 -0.6 208,043 - MPMX19_06047 0.30 +0.7 208,043 - MPMX19_06047 0.30 -1.1 208,192 + MPMX19_06047 0.42 -0.9 208,192 + MPMX19_06047 0.42 +0.1 208,192 + MPMX19_06047 0.42 -1.3 208,192 + MPMX19_06047 0.42 +1.1 208,192 + MPMX19_06047 0.42 +1.1 208,193 - MPMX19_06047 0.42 -0.1 208,193 - MPMX19_06047 0.42 -1.3 208,193 - MPMX19_06047 0.42 -0.5 208,193 - MPMX19_06047 0.42 +0.5 208,193 - MPMX19_06047 0.42 +0.9 208,204 + MPMX19_06047 0.43 +0.1 208,204 + MPMX19_06047 0.43 +0.3 208,204 + MPMX19_06047 0.43 +1.3 208,204 + MPMX19_06047 0.43 -0.3 208,205 - MPMX19_06047 0.43 -2.5 208,205 - MPMX19_06047 0.43 +0.1 208,205 - MPMX19_06047 0.43 +1.7 208,213 + MPMX19_06047 0.44 +0.1 208,213 + MPMX19_06047 0.44 -0.5 208,213 + MPMX19_06047 0.44 +0.3 208,213 + MPMX19_06047 0.44 -1.0 208,213 + MPMX19_06047 0.44 -0.6 208,213 + MPMX19_06047 0.44 -2.3 208,213 + MPMX19_06047 0.44 -1.3 208,213 + MPMX19_06047 0.44 -1.3 208,214 - MPMX19_06047 0.44 -0.6 208,214 - MPMX19_06047 0.44 -0.3 208,214 - MPMX19_06047 0.44 -0.3 208,214 - MPMX19_06047 0.44 +0.1 208,342 + MPMX19_06047 0.55 +0.0 208,342 + MPMX19_06047 0.55 +0.1 208,343 - MPMX19_06047 0.55 -0.2 208,343 - MPMX19_06047 0.55 +0.1 208,905 + -0.9 208,906 - -0.0 208,906 - -0.3 208,906 - -0.7 208,906 - -0.0 208,964 - +1.2 209,012 + -0.9 209,012 + -0.3 209,012 + -2.3 209,013 - +0.4 209,013 - -0.3 209,048 + -1.3 209,048 + -0.9 209,048 + +0.3 209,048 + -0.5 209,048 + -1.0 209,049 - -0.9 209,049 - -0.5 209,049 - +0.7 209,049 - +0.7 209,049 - -0.9 209,049 - -0.7 209,074 - -0.2 209,204 + +1.0 209,204 + -0.9 209,204 + -0.7 209,204 + +0.2 209,204 + -0.9 209,204 + -1.2 209,204 + +0.2 209,204 + +0.1 209,204 + -0.5 209,205 - -0.5 209,205 - -1.5 209,205 - -0.7 209,205 - -0.7 209,205 - -1.0 209,205 - +1.5 209,205 - -0.5 209,205 - -1.3 209,205 - -0.7 209,205 - -0.1 209,205 - -0.3 209,512 - MPMX19_06048 0.12 +1.1 209,512 - MPMX19_06048 0.12 +1.7 209,512 - MPMX19_06048 0.12 -1.7 209,512 - MPMX19_06048 0.12 -0.4 209,512 - MPMX19_06048 0.12 -0.3 209,512 - MPMX19_06048 0.12 -1.4 209,718 + MPMX19_06048 0.19 -0.6 209,718 + MPMX19_06048 0.19 +0.3 209,718 + MPMX19_06048 0.19 +0.5 209,718 + MPMX19_06048 0.19 +1.0 209,719 - MPMX19_06048 0.19 -0.5 209,719 - MPMX19_06048 0.19 -0.1 209,719 - MPMX19_06048 0.19 -0.7 209,719 - MPMX19_06048 0.19 -0.5 209,719 - MPMX19_06048 0.19 +0.7 209,859 + MPMX19_06048 0.25 -0.8 209,859 + MPMX19_06048 0.25 -0.5 209,859 + MPMX19_06048 0.25 +1.0 209,859 + MPMX19_06048 0.25 +1.1 209,859 + MPMX19_06048 0.25 -0.3 209,859 + MPMX19_06048 0.25 +1.2 209,859 + MPMX19_06048 0.25 -0.2 209,859 + MPMX19_06048 0.25 +0.1 209,859 + MPMX19_06048 0.25 -2.3 209,859 + MPMX19_06048 0.25 +1.7 209,859 + MPMX19_06048 0.25 +0.2 209,859 + MPMX19_06048 0.25 -0.1 209,859 + MPMX19_06048 0.25 -0.1 209,859 + MPMX19_06048 0.25 -1.3 209,860 - MPMX19_06048 0.25 +0.1 209,860 - MPMX19_06048 0.25 -1.4 209,860 - MPMX19_06048 0.25 -0.5 209,860 - MPMX19_06048 0.25 -0.3 209,860 - MPMX19_06048 0.25 -0.1 209,860 - MPMX19_06048 0.25 -0.2 209,860 - MPMX19_06048 0.25 -0.8 209,860 - MPMX19_06048 0.25 +0.4 209,860 - MPMX19_06048 0.25 +0.1
Or see this region's nucleotide sequence