Experiment: EMM_noCarbon with Sucrose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1458 and GFF1459 are separated by 121 nucleotides GFF1459 and GFF1460 are separated by 64 nucleotides GFF1460 and GFF1461 overlap by 4 nucleotides GFF1461 and GFF1462 are separated by 7 nucleotides
GFF1458 - Replicative DNA helicase (DnaB) (EC 3.6.4.12), at 1,486,272 to 1,487,759
GFF1458
GFF1459 - hypothetical protein, at 1,487,881 to 1,488,363
GFF1459
GFF1460 - glycoside hydrolase, family 25, at 1,488,428 to 1,489,060
GFF1460
GFF1461 - Cytidine deaminase (EC 3.5.4.5), at 1,489,057 to 1,489,530
GFF1461
GFF1462 - Deoxycytidine triphosphate deaminase (EC 3.5.4.13), at 1,489,538 to 1,490,092
GFF1462
Position (kb)
1488
1489
1490 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1487.786 kb on - strand at 1487.795 kb on + strand at 1487.795 kb on + strand at 1487.795 kb on + strand at 1487.801 kb on + strand at 1487.801 kb on + strand at 1487.803 kb on - strand at 1487.803 kb on - strand at 1487.809 kb on - strand at 1487.856 kb on + strand at 1487.863 kb on + strand at 1487.868 kb on + strand at 1487.868 kb on + strand at 1487.871 kb on - strand at 1487.876 kb on - strand at 1487.876 kb on - strand at 1487.876 kb on - strand at 1487.904 kb on + strand at 1487.905 kb on - strand at 1487.909 kb on + strand at 1487.924 kb on + strand at 1487.926 kb on + strand at 1487.934 kb on + strand, within GFF1459 at 1487.939 kb on + strand, within GFF1459 at 1487.982 kb on + strand, within GFF1459 at 1488.020 kb on - strand, within GFF1459 at 1488.039 kb on + strand, within GFF1459 at 1488.039 kb on + strand, within GFF1459 at 1488.039 kb on + strand, within GFF1459 at 1488.039 kb on + strand, within GFF1459 at 1488.041 kb on + strand, within GFF1459 at 1488.041 kb on + strand, within GFF1459 at 1488.042 kb on - strand, within GFF1459 at 1488.046 kb on - strand, within GFF1459 at 1488.047 kb on - strand, within GFF1459 at 1488.047 kb on - strand, within GFF1459 at 1488.047 kb on - strand, within GFF1459 at 1488.047 kb on - strand, within GFF1459 at 1488.121 kb on + strand, within GFF1459 at 1488.122 kb on + strand, within GFF1459 at 1488.122 kb on + strand, within GFF1459 at 1488.122 kb on + strand, within GFF1459 at 1488.122 kb on + strand, within GFF1459 at 1488.127 kb on + strand, within GFF1459 at 1488.179 kb on + strand, within GFF1459 at 1488.185 kb on - strand, within GFF1459 at 1488.197 kb on + strand, within GFF1459 at 1488.197 kb on + strand, within GFF1459 at 1488.201 kb on + strand, within GFF1459 at 1488.209 kb on - strand, within GFF1459 at 1488.216 kb on + strand, within GFF1459 at 1488.224 kb on + strand, within GFF1459 at 1488.224 kb on - strand, within GFF1459 at 1488.224 kb on - strand, within GFF1459 at 1488.224 kb on - strand, within GFF1459 at 1488.233 kb on + strand, within GFF1459 at 1488.235 kb on + strand, within GFF1459 at 1488.252 kb on + strand, within GFF1459 at 1488.269 kb on + strand, within GFF1459 at 1488.277 kb on - strand, within GFF1459 at 1488.304 kb on + strand, within GFF1459 at 1488.309 kb on - strand, within GFF1459 at 1488.327 kb on + strand at 1488.327 kb on + strand at 1488.335 kb on - strand at 1488.335 kb on - strand at 1488.367 kb on + strand at 1488.402 kb on + strand at 1488.431 kb on + strand at 1488.438 kb on + strand at 1488.438 kb on + strand at 1488.444 kb on - strand at 1488.444 kb on - strand at 1488.446 kb on - strand at 1488.446 kb on - strand at 1488.454 kb on - strand at 1488.494 kb on + strand, within GFF1460 at 1488.508 kb on + strand, within GFF1460 at 1488.514 kb on + strand, within GFF1460 at 1488.522 kb on - strand, within GFF1460 at 1488.592 kb on - strand, within GFF1460 at 1488.650 kb on - strand, within GFF1460 at 1488.665 kb on + strand, within GFF1460 at 1488.665 kb on + strand, within GFF1460 at 1488.665 kb on + strand, within GFF1460 at 1488.665 kb on + strand, within GFF1460 at 1488.670 kb on + strand, within GFF1460 at 1488.670 kb on + strand, within GFF1460 at 1488.670 kb on + strand, within GFF1460 at 1488.672 kb on + strand, within GFF1460 at 1488.672 kb on + strand, within GFF1460 at 1488.673 kb on - strand, within GFF1460 at 1488.678 kb on - strand, within GFF1460 at 1488.678 kb on - strand, within GFF1460 at 1488.680 kb on - strand, within GFF1460 at 1488.680 kb on - strand, within GFF1460 at 1488.707 kb on + strand, within GFF1460 at 1488.712 kb on + strand, within GFF1460 at 1488.748 kb on + strand, within GFF1460 at 1488.748 kb on + strand, within GFF1460 at 1488.748 kb on + strand, within GFF1460 at 1488.748 kb on + strand, within GFF1460 at 1488.753 kb on + strand, within GFF1460 at 1488.756 kb on - strand, within GFF1460 at 1488.778 kb on + strand, within GFF1460 at 1488.778 kb on + strand, within GFF1460 at 1488.786 kb on - strand, within GFF1460 at 1488.832 kb on + strand, within GFF1460 at 1488.879 kb on - strand, within GFF1460 at 1488.922 kb on + strand, within GFF1460 at 1488.930 kb on - strand, within GFF1460 at 1488.976 kb on + strand, within GFF1460 at 1488.976 kb on + strand, within GFF1460 at 1488.976 kb on + strand, within GFF1460 at 1488.989 kb on - strand, within GFF1460 at 1488.991 kb on - strand, within GFF1460 at 1489.038 kb on + strand at 1489.038 kb on + strand at 1489.046 kb on - strand at 1489.056 kb on + strand at 1489.089 kb on + strand at 1489.089 kb on + strand at 1489.115 kb on + strand, within GFF1461 at 1489.129 kb on + strand, within GFF1461 at 1489.168 kb on + strand, within GFF1461 at 1489.211 kb on + strand, within GFF1461 at 1489.211 kb on + strand, within GFF1461 at 1489.274 kb on - strand, within GFF1461 at 1489.354 kb on + strand, within GFF1461 at 1489.385 kb on + strand, within GFF1461 at 1489.416 kb on + strand, within GFF1461 at 1489.427 kb on - strand, within GFF1461 at 1489.450 kb on + strand, within GFF1461 at 1489.450 kb on + strand, within GFF1461 at 1489.458 kb on - strand, within GFF1461 at 1489.471 kb on + strand, within GFF1461 at 1489.477 kb on + strand, within GFF1461 at 1489.484 kb on + strand at 1489.504 kb on + strand at 1489.511 kb on + strand at 1489.511 kb on + strand at 1489.511 kb on + strand at 1489.511 kb on + strand at 1489.514 kb on + strand at 1489.519 kb on - strand at 1489.535 kb on + strand at 1489.543 kb on - strand at 1489.569 kb on + strand at 1489.658 kb on - strand, within GFF1462 at 1489.688 kb on + strand, within GFF1462 at 1489.773 kb on - strand, within GFF1462 at 1489.883 kb on + strand, within GFF1462 at 1489.900 kb on + strand, within GFF1462 at 1490.034 kb on + strand, within GFF1462
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Ammonium Sulfate remove 1,487,786 - -0.7 1,487,795 + -1.0 1,487,795 + +0.7 1,487,795 + -1.4 1,487,801 + -1.0 1,487,801 + -1.4 1,487,803 - -0.2 1,487,803 - -1.7 1,487,809 - +0.0 1,487,856 + -0.5 1,487,863 + +0.8 1,487,868 + -0.8 1,487,868 + -0.8 1,487,871 - -0.8 1,487,876 - +1.2 1,487,876 - +0.9 1,487,876 - -0.3 1,487,904 + -0.1 1,487,905 - -1.4 1,487,909 + -0.7 1,487,924 + +1.4 1,487,926 + +0.3 1,487,934 + GFF1459 0.11 -0.0 1,487,939 + GFF1459 0.12 +1.6 1,487,982 + GFF1459 0.21 +0.0 1,488,020 - GFF1459 0.29 -1.4 1,488,039 + GFF1459 0.33 -0.9 1,488,039 + GFF1459 0.33 -0.2 1,488,039 + GFF1459 0.33 +0.6 1,488,039 + GFF1459 0.33 +0.6 1,488,041 + GFF1459 0.33 -0.6 1,488,041 + GFF1459 0.33 +0.3 1,488,042 - GFF1459 0.33 -0.1 1,488,046 - GFF1459 0.34 +1.9 1,488,047 - GFF1459 0.34 -0.4 1,488,047 - GFF1459 0.34 -0.0 1,488,047 - GFF1459 0.34 +0.0 1,488,047 - GFF1459 0.34 -0.1 1,488,121 + GFF1459 0.50 -0.2 1,488,122 + GFF1459 0.50 -0.6 1,488,122 + GFF1459 0.50 -0.2 1,488,122 + GFF1459 0.50 -0.9 1,488,122 + GFF1459 0.50 -0.2 1,488,127 + GFF1459 0.51 +0.5 1,488,179 + GFF1459 0.62 +0.3 1,488,185 - GFF1459 0.63 -0.8 1,488,197 + GFF1459 0.65 -0.9 1,488,197 + GFF1459 0.65 +0.1 1,488,201 + GFF1459 0.66 +0.9 1,488,209 - GFF1459 0.68 -1.2 1,488,216 + GFF1459 0.69 -0.7 1,488,224 + GFF1459 0.71 -0.2 1,488,224 - GFF1459 0.71 -0.1 1,488,224 - GFF1459 0.71 -1.7 1,488,224 - GFF1459 0.71 -1.1 1,488,233 + GFF1459 0.73 +0.8 1,488,235 + GFF1459 0.73 -2.5 1,488,252 + GFF1459 0.77 -0.4 1,488,269 + GFF1459 0.80 +0.1 1,488,277 - GFF1459 0.82 +0.6 1,488,304 + GFF1459 0.88 +0.9 1,488,309 - GFF1459 0.89 -0.4 1,488,327 + -0.2 1,488,327 + +0.5 1,488,335 - -0.7 1,488,335 - -1.1 1,488,367 + -1.7 1,488,402 + -0.3 1,488,431 + +0.9 1,488,438 + +0.3 1,488,438 + -0.4 1,488,444 - -0.7 1,488,444 - -0.5 1,488,446 - -0.7 1,488,446 - -0.8 1,488,454 - -0.1 1,488,494 + GFF1460 0.10 -0.5 1,488,508 + GFF1460 0.13 -1.2 1,488,514 + GFF1460 0.14 -0.6 1,488,522 - GFF1460 0.15 -0.8 1,488,592 - GFF1460 0.26 -0.1 1,488,650 - GFF1460 0.35 +0.3 1,488,665 + GFF1460 0.37 -0.2 1,488,665 + GFF1460 0.37 -0.6 1,488,665 + GFF1460 0.37 +0.8 1,488,665 + GFF1460 0.37 -0.4 1,488,670 + GFF1460 0.38 -0.8 1,488,670 + GFF1460 0.38 -0.7 1,488,670 + GFF1460 0.38 -0.1 1,488,672 + GFF1460 0.39 -0.7 1,488,672 + GFF1460 0.39 +0.3 1,488,673 - GFF1460 0.39 +0.8 1,488,678 - GFF1460 0.39 -0.2 1,488,678 - GFF1460 0.39 -0.4 1,488,680 - GFF1460 0.40 -0.4 1,488,680 - GFF1460 0.40 -0.4 1,488,707 + GFF1460 0.44 -0.2 1,488,712 + GFF1460 0.45 -0.4 1,488,748 + GFF1460 0.51 +0.6 1,488,748 + GFF1460 0.51 -0.1 1,488,748 + GFF1460 0.51 +0.3 1,488,748 + GFF1460 0.51 -0.2 1,488,753 + GFF1460 0.51 -0.0 1,488,756 - GFF1460 0.52 +2.3 1,488,778 + GFF1460 0.55 +0.3 1,488,778 + GFF1460 0.55 -0.8 1,488,786 - GFF1460 0.57 -0.6 1,488,832 + GFF1460 0.64 -0.9 1,488,879 - GFF1460 0.71 -0.2 1,488,922 + GFF1460 0.78 +2.0 1,488,930 - GFF1460 0.79 -0.7 1,488,976 + GFF1460 0.87 -1.5 1,488,976 + GFF1460 0.87 -0.1 1,488,976 + GFF1460 0.87 +0.5 1,488,989 - GFF1460 0.89 -0.5 1,488,991 - GFF1460 0.89 -0.9 1,489,038 + +0.2 1,489,038 + -0.8 1,489,046 - -1.6 1,489,056 + +0.3 1,489,089 + -0.4 1,489,089 + -0.4 1,489,115 + GFF1461 0.12 -0.7 1,489,129 + GFF1461 0.15 +0.7 1,489,168 + GFF1461 0.23 -0.2 1,489,211 + GFF1461 0.32 -0.7 1,489,211 + GFF1461 0.32 -0.2 1,489,274 - GFF1461 0.46 -0.1 1,489,354 + GFF1461 0.63 -0.8 1,489,385 + GFF1461 0.69 +0.8 1,489,416 + GFF1461 0.76 -0.1 1,489,427 - GFF1461 0.78 -0.8 1,489,450 + GFF1461 0.83 -0.0 1,489,450 + GFF1461 0.83 +1.4 1,489,458 - GFF1461 0.85 -0.5 1,489,471 + GFF1461 0.87 +0.2 1,489,477 + GFF1461 0.89 +1.0 1,489,484 + -0.6 1,489,504 + -1.2 1,489,511 + +0.8 1,489,511 + -2.0 1,489,511 + -0.7 1,489,511 + -0.5 1,489,514 + -0.6 1,489,519 - -0.2 1,489,535 + -0.2 1,489,543 - -0.2 1,489,569 + -0.1 1,489,658 - GFF1462 0.22 -0.6 1,489,688 + GFF1462 0.27 -1.1 1,489,773 - GFF1462 0.42 -0.1 1,489,883 + GFF1462 0.62 +0.5 1,489,900 + GFF1462 0.65 -1.0 1,490,034 + GFF1462 0.89 +1.3
Or see this region's nucleotide sequence