Experiment: EMM_noCarbon with Sucrose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF61 and GFF62 are separated by 139 nucleotides GFF62 and GFF63 are separated by 105 nucleotides GFF63 and GFF64 are separated by 93 nucleotides GFF64 and GFF65 are separated by 120 nucleotides GFF65 and GFF66 are separated by 72 nucleotides
GFF61 - hypothetical protein, at 53,166 to 53,657
GFF61
GFF62 - tRNA-Arg-ACG, at 53,797 to 53,873
GFF62
GFF63 - hypothetical protein, at 53,979 to 54,158
GFF63
GFF64 - hypothetical protein, at 54,252 to 54,434
GFF64
GFF65 - tRNA-Arg-ACG, at 54,555 to 54,631
GFF65
GFF66 - Cobyric acid synthase (EC 6.3.5.10), at 54,704 to 56,152
GFF66
Position (kb) 54
55 Strain fitness (log2 ratio) -2
-1
0
1
2 at 53.307 kb on + strand, within GFF61 at 53.414 kb on - strand, within GFF61 at 53.517 kb on - strand, within GFF61 at 53.605 kb on + strand, within GFF61 at 53.622 kb on - strand at 53.630 kb on + strand at 53.712 kb on + strand at 53.812 kb on - strand, within GFF62 at 53.907 kb on + strand at 53.981 kb on + strand at 53.988 kb on + strand at 53.988 kb on + strand at 53.988 kb on + strand at 53.996 kb on - strand at 54.010 kb on + strand, within GFF63 at 54.035 kb on + strand, within GFF63 at 54.043 kb on - strand, within GFF63 at 54.044 kb on - strand, within GFF63 at 54.075 kb on + strand, within GFF63 at 54.092 kb on + strand, within GFF63 at 54.105 kb on + strand, within GFF63 at 54.105 kb on + strand, within GFF63 at 54.113 kb on - strand, within GFF63 at 54.113 kb on - strand, within GFF63 at 54.119 kb on + strand, within GFF63 at 54.127 kb on - strand, within GFF63 at 54.159 kb on + strand at 54.198 kb on + strand at 54.259 kb on + strand at 54.262 kb on - strand at 54.301 kb on - strand, within GFF64 at 54.337 kb on - strand, within GFF64 at 54.369 kb on + strand, within GFF64 at 54.428 kb on + strand at 54.548 kb on + strand at 54.794 kb on + strand at 54.802 kb on - strand at 54.903 kb on + strand, within GFF66 at 55.020 kb on + strand, within GFF66 at 55.020 kb on + strand, within GFF66 at 55.038 kb on + strand, within GFF66 at 55.038 kb on - strand, within GFF66 at 55.073 kb on - strand, within GFF66 at 55.073 kb on - strand, within GFF66 at 55.075 kb on + strand, within GFF66 at 55.083 kb on - strand, within GFF66 at 55.083 kb on - strand, within GFF66 at 55.083 kb on - strand, within GFF66 at 55.097 kb on + strand, within GFF66 at 55.199 kb on + strand, within GFF66 at 55.209 kb on + strand, within GFF66 at 55.209 kb on + strand, within GFF66 at 55.209 kb on + strand, within GFF66 at 55.209 kb on + strand, within GFF66 at 55.217 kb on - strand, within GFF66 at 55.230 kb on + strand, within GFF66 at 55.252 kb on + strand, within GFF66 at 55.260 kb on - strand, within GFF66 at 55.289 kb on - strand, within GFF66 at 55.321 kb on + strand, within GFF66 at 55.327 kb on + strand, within GFF66 at 55.327 kb on + strand, within GFF66 at 55.346 kb on - strand, within GFF66 at 55.348 kb on + strand, within GFF66 at 55.376 kb on + strand, within GFF66 at 55.389 kb on - strand, within GFF66
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Ammonium Sulfate remove 53,307 + GFF61 0.29 +1.4 53,414 - GFF61 0.50 -1.1 53,517 - GFF61 0.71 -0.5 53,605 + GFF61 0.89 -0.9 53,622 - -0.4 53,630 + +1.3 53,712 + +0.1 53,812 - GFF62 0.19 -0.1 53,907 + -0.3 53,981 + +0.4 53,988 + -1.0 53,988 + -0.3 53,988 + -1.8 53,996 - -0.1 54,010 + GFF63 0.17 +0.4 54,035 + GFF63 0.31 -0.3 54,043 - GFF63 0.36 +1.4 54,044 - GFF63 0.36 +0.2 54,075 + GFF63 0.53 +0.4 54,092 + GFF63 0.63 +0.6 54,105 + GFF63 0.70 +2.6 54,105 + GFF63 0.70 +0.4 54,113 - GFF63 0.74 -1.3 54,113 - GFF63 0.74 -1.1 54,119 + GFF63 0.78 +0.0 54,127 - GFF63 0.82 +0.0 54,159 + -0.9 54,198 + +1.2 54,259 + +1.4 54,262 - -0.7 54,301 - GFF64 0.27 -1.0 54,337 - GFF64 0.46 -1.1 54,369 + GFF64 0.64 -1.8 54,428 + -0.3 54,548 + +0.1 54,794 + -1.4 54,802 - +0.6 54,903 + GFF66 0.14 +1.3 55,020 + GFF66 0.22 -0.2 55,020 + GFF66 0.22 +1.0 55,038 + GFF66 0.23 -0.1 55,038 - GFF66 0.23 +1.0 55,073 - GFF66 0.25 -0.9 55,073 - GFF66 0.25 -1.0 55,075 + GFF66 0.26 +0.3 55,083 - GFF66 0.26 -0.5 55,083 - GFF66 0.26 -0.2 55,083 - GFF66 0.26 -0.9 55,097 + GFF66 0.27 -0.6 55,199 + GFF66 0.34 -0.4 55,209 + GFF66 0.35 +1.6 55,209 + GFF66 0.35 -0.6 55,209 + GFF66 0.35 +1.0 55,209 + GFF66 0.35 +0.1 55,217 - GFF66 0.35 -0.6 55,230 + GFF66 0.36 +0.1 55,252 + GFF66 0.38 -1.2 55,260 - GFF66 0.38 -0.1 55,289 - GFF66 0.40 -1.4 55,321 + GFF66 0.43 -1.5 55,327 + GFF66 0.43 +0.1 55,327 + GFF66 0.43 +0.4 55,346 - GFF66 0.44 +0.0 55,348 + GFF66 0.44 +0.8 55,376 + GFF66 0.46 +1.0 55,389 - GFF66 0.47 -0.3
Or see this region's nucleotide sequence