Experiment: EMM_noCarbon with Sucrose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF59 and GFF60 are separated by 138 nucleotides GFF60 and GFF61 are separated by 46 nucleotides GFF61 and GFF62 are separated by 139 nucleotides GFF62 and GFF63 are separated by 105 nucleotides
GFF59 - Glycyl-tRNA synthetase beta chain (EC 6.1.1.14), at 48,077 to 50,281
GFF59
GFF60 - Pyruvate,phosphate dikinase (EC 2.7.9.1), at 50,420 to 53,119
GFF60
GFF61 - hypothetical protein, at 53,166 to 53,657
GFF61
GFF62 - tRNA-Arg-ACG, at 53,797 to 53,873
GFF62
GFF63 - hypothetical protein, at 53,979 to 54,158
GFF63
Position (kb)
50
51
52
53
54 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 50.327 kb on + strand at 50.327 kb on + strand at 50.333 kb on + strand at 50.335 kb on - strand at 50.335 kb on - strand at 50.382 kb on + strand at 50.384 kb on + strand at 50.401 kb on + strand at 50.416 kb on + strand at 50.423 kb on - strand at 50.424 kb on - strand at 50.427 kb on + strand at 50.427 kb on + strand at 50.438 kb on + strand at 50.438 kb on + strand at 50.446 kb on - strand at 50.446 kb on - strand at 50.446 kb on - strand at 50.454 kb on - strand at 50.508 kb on - strand at 50.535 kb on + strand at 50.543 kb on - strand at 50.614 kb on + strand at 50.679 kb on + strand at 50.731 kb on + strand, within GFF60 at 50.731 kb on + strand, within GFF60 at 50.732 kb on + strand, within GFF60 at 50.732 kb on + strand, within GFF60 at 50.742 kb on + strand, within GFF60 at 50.767 kb on + strand, within GFF60 at 50.783 kb on + strand, within GFF60 at 50.783 kb on + strand, within GFF60 at 50.783 kb on + strand, within GFF60 at 50.783 kb on + strand, within GFF60 at 50.783 kb on + strand, within GFF60 at 50.786 kb on - strand, within GFF60 at 50.791 kb on - strand, within GFF60 at 50.791 kb on - strand, within GFF60 at 50.791 kb on - strand, within GFF60 at 50.798 kb on - strand, within GFF60 at 50.798 kb on - strand, within GFF60 at 50.812 kb on - strand, within GFF60 at 50.816 kb on - strand, within GFF60 at 50.821 kb on - strand, within GFF60 at 50.821 kb on - strand, within GFF60 at 50.821 kb on - strand, within GFF60 at 50.821 kb on - strand, within GFF60 at 50.821 kb on - strand, within GFF60 at 50.826 kb on - strand, within GFF60 at 50.860 kb on - strand, within GFF60 at 50.876 kb on + strand, within GFF60 at 50.964 kb on - strand, within GFF60 at 51.034 kb on + strand, within GFF60 at 51.034 kb on + strand, within GFF60 at 51.040 kb on + strand, within GFF60 at 51.073 kb on + strand, within GFF60 at 51.073 kb on + strand, within GFF60 at 51.081 kb on - strand, within GFF60 at 51.103 kb on - strand, within GFF60 at 51.128 kb on + strand, within GFF60 at 51.128 kb on + strand, within GFF60 at 51.136 kb on - strand, within GFF60 at 51.163 kb on + strand, within GFF60 at 51.179 kb on + strand, within GFF60 at 51.179 kb on + strand, within GFF60 at 51.191 kb on + strand, within GFF60 at 51.191 kb on + strand, within GFF60 at 51.205 kb on + strand, within GFF60 at 51.205 kb on + strand, within GFF60 at 51.205 kb on + strand, within GFF60 at 51.205 kb on + strand, within GFF60 at 51.230 kb on + strand, within GFF60 at 51.230 kb on + strand, within GFF60 at 51.230 kb on + strand, within GFF60 at 51.230 kb on + strand, within GFF60 at 51.230 kb on + strand, within GFF60 at 51.233 kb on - strand, within GFF60 at 51.238 kb on - strand, within GFF60 at 51.238 kb on - strand, within GFF60 at 51.238 kb on - strand, within GFF60 at 51.238 kb on - strand, within GFF60 at 51.278 kb on + strand, within GFF60 at 51.283 kb on + strand, within GFF60 at 51.316 kb on + strand, within GFF60 at 51.349 kb on - strand, within GFF60 at 51.409 kb on - strand, within GFF60 at 51.455 kb on + strand, within GFF60 at 51.463 kb on - strand, within GFF60 at 51.476 kb on + strand, within GFF60 at 51.484 kb on - strand, within GFF60 at 51.485 kb on + strand, within GFF60 at 51.490 kb on + strand, within GFF60 at 51.490 kb on + strand, within GFF60 at 51.498 kb on - strand, within GFF60 at 51.498 kb on - strand, within GFF60 at 51.498 kb on - strand, within GFF60 at 51.500 kb on + strand, within GFF60 at 51.500 kb on + strand, within GFF60 at 51.500 kb on + strand, within GFF60 at 51.500 kb on + strand, within GFF60 at 51.500 kb on + strand, within GFF60 at 51.500 kb on + strand, within GFF60 at 51.500 kb on + strand, within GFF60 at 51.500 kb on + strand, within GFF60 at 51.500 kb on + strand, within GFF60 at 51.500 kb on + strand, within GFF60 at 51.508 kb on - strand, within GFF60 at 51.508 kb on - strand, within GFF60 at 51.508 kb on - strand, within GFF60 at 51.508 kb on - strand, within GFF60 at 51.508 kb on - strand, within GFF60 at 51.508 kb on - strand, within GFF60 at 51.508 kb on - strand, within GFF60 at 51.508 kb on - strand, within GFF60 at 51.518 kb on - strand, within GFF60 at 51.523 kb on - strand, within GFF60 at 51.523 kb on - strand, within GFF60 at 51.525 kb on + strand, within GFF60 at 51.534 kb on - strand, within GFF60 at 51.538 kb on + strand, within GFF60 at 51.570 kb on + strand, within GFF60 at 51.574 kb on + strand, within GFF60 at 51.577 kb on - strand, within GFF60 at 51.577 kb on - strand, within GFF60 at 51.582 kb on - strand, within GFF60 at 51.598 kb on - strand, within GFF60 at 51.617 kb on - strand, within GFF60 at 51.636 kb on - strand, within GFF60 at 51.665 kb on + strand, within GFF60 at 51.677 kb on + strand, within GFF60 at 51.754 kb on + strand, within GFF60 at 51.754 kb on + strand, within GFF60 at 51.754 kb on + strand, within GFF60 at 51.767 kb on + strand, within GFF60 at 51.779 kb on + strand, within GFF60 at 51.818 kb on + strand, within GFF60 at 51.822 kb on + strand, within GFF60 at 51.831 kb on - strand, within GFF60 at 51.834 kb on - strand, within GFF60 at 51.834 kb on - strand, within GFF60 at 51.845 kb on + strand, within GFF60 at 51.883 kb on + strand, within GFF60 at 51.884 kb on + strand, within GFF60 at 51.884 kb on + strand, within GFF60 at 51.887 kb on - strand, within GFF60 at 51.891 kb on - strand, within GFF60 at 51.892 kb on - strand, within GFF60 at 51.938 kb on + strand, within GFF60 at 51.944 kb on - strand, within GFF60 at 51.964 kb on + strand, within GFF60 at 51.972 kb on + strand, within GFF60 at 51.977 kb on + strand, within GFF60 at 51.977 kb on + strand, within GFF60 at 51.977 kb on + strand, within GFF60 at 51.977 kb on + strand, within GFF60 at 51.985 kb on - strand, within GFF60 at 52.007 kb on - strand, within GFF60 at 52.015 kb on - strand, within GFF60 at 52.043 kb on - strand, within GFF60 at 52.069 kb on + strand, within GFF60 at 52.092 kb on + strand, within GFF60 at 52.092 kb on - strand, within GFF60 at 52.129 kb on - strand, within GFF60 at 52.131 kb on + strand, within GFF60 at 52.137 kb on - strand, within GFF60 at 52.137 kb on - strand, within GFF60 at 52.161 kb on + strand, within GFF60 at 52.161 kb on + strand, within GFF60 at 52.168 kb on - strand, within GFF60 at 52.171 kb on + strand, within GFF60 at 52.171 kb on + strand, within GFF60 at 52.171 kb on + strand, within GFF60 at 52.171 kb on + strand, within GFF60 at 52.171 kb on + strand, within GFF60 at 52.171 kb on + strand, within GFF60 at 52.171 kb on + strand, within GFF60 at 52.171 kb on + strand, within GFF60 at 52.171 kb on + strand, within GFF60 at 52.174 kb on - strand, within GFF60 at 52.174 kb on - strand, within GFF60 at 52.179 kb on - strand, within GFF60 at 52.179 kb on - strand, within GFF60 at 52.179 kb on - strand, within GFF60 at 52.179 kb on - strand, within GFF60 at 52.179 kb on - strand, within GFF60 at 52.181 kb on + strand, within GFF60 at 52.202 kb on + strand, within GFF60 at 52.209 kb on + strand, within GFF60 at 52.210 kb on - strand, within GFF60 at 52.217 kb on - strand, within GFF60 at 52.224 kb on + strand, within GFF60 at 52.224 kb on + strand, within GFF60 at 52.224 kb on + strand, within GFF60 at 52.282 kb on - strand, within GFF60 at 52.316 kb on - strand, within GFF60 at 52.320 kb on + strand, within GFF60 at 52.328 kb on - strand, within GFF60 at 52.338 kb on + strand, within GFF60 at 52.338 kb on - strand, within GFF60 at 52.338 kb on - strand, within GFF60 at 52.338 kb on - strand, within GFF60 at 52.338 kb on - strand, within GFF60 at 52.339 kb on - strand, within GFF60 at 52.346 kb on - strand, within GFF60 at 52.346 kb on - strand, within GFF60 at 52.348 kb on + strand, within GFF60 at 52.405 kb on + strand, within GFF60 at 52.405 kb on + strand, within GFF60 at 52.405 kb on + strand, within GFF60 at 52.418 kb on + strand, within GFF60 at 52.425 kb on + strand, within GFF60 at 52.425 kb on + strand, within GFF60 at 52.457 kb on - strand, within GFF60 at 52.483 kb on - strand, within GFF60 at 52.502 kb on + strand, within GFF60 at 52.510 kb on - strand, within GFF60 at 52.512 kb on + strand, within GFF60 at 52.546 kb on + strand, within GFF60 at 52.552 kb on - strand, within GFF60 at 52.554 kb on - strand, within GFF60 at 52.576 kb on + strand, within GFF60 at 52.601 kb on + strand, within GFF60 at 52.634 kb on - strand, within GFF60 at 52.642 kb on + strand, within GFF60 at 52.642 kb on + strand, within GFF60 at 52.750 kb on - strand, within GFF60 at 52.794 kb on + strand, within GFF60 at 52.794 kb on - strand, within GFF60 at 52.835 kb on - strand, within GFF60 at 52.914 kb on + strand at 52.914 kb on + strand at 52.927 kb on - strand at 52.986 kb on + strand at 53.062 kb on - strand at 53.064 kb on + strand at 53.139 kb on + strand at 53.164 kb on + strand at 53.164 kb on + strand at 53.164 kb on + strand at 53.164 kb on + strand at 53.195 kb on + strand at 53.244 kb on - strand, within GFF61 at 53.307 kb on + strand, within GFF61 at 53.414 kb on - strand, within GFF61 at 53.517 kb on - strand, within GFF61 at 53.605 kb on + strand, within GFF61 at 53.622 kb on - strand at 53.630 kb on + strand at 53.712 kb on + strand at 53.812 kb on - strand, within GFF62 at 53.907 kb on + strand at 53.981 kb on + strand at 53.988 kb on + strand at 53.988 kb on + strand at 53.988 kb on + strand at 53.996 kb on - strand at 54.010 kb on + strand, within GFF63 at 54.035 kb on + strand, within GFF63 at 54.043 kb on - strand, within GFF63 at 54.044 kb on - strand, within GFF63 at 54.075 kb on + strand, within GFF63 at 54.092 kb on + strand, within GFF63 at 54.105 kb on + strand, within GFF63 at 54.105 kb on + strand, within GFF63 at 54.113 kb on - strand, within GFF63 at 54.113 kb on - strand, within GFF63 at 54.119 kb on + strand, within GFF63
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Ammonium Sulfate remove 50,327 + -0.1 50,327 + -0.4 50,333 + -0.1 50,335 - +1.1 50,335 - -0.2 50,382 + -0.4 50,384 + -0.7 50,401 + +0.3 50,416 + -0.9 50,423 - -0.9 50,424 - +0.1 50,427 + -0.0 50,427 + -0.9 50,438 + -1.5 50,438 + -1.0 50,446 - +0.6 50,446 - -0.1 50,446 - +0.3 50,454 - -0.7 50,508 - -1.7 50,535 + -0.9 50,543 - -2.1 50,614 + -1.0 50,679 + +0.8 50,731 + GFF60 0.12 -0.6 50,731 + GFF60 0.12 -1.1 50,732 + GFF60 0.12 -0.1 50,732 + GFF60 0.12 -2.3 50,742 + GFF60 0.12 -1.8 50,767 + GFF60 0.13 -0.1 50,783 + GFF60 0.13 +0.4 50,783 + GFF60 0.13 -1.6 50,783 + GFF60 0.13 -1.7 50,783 + GFF60 0.13 -1.1 50,783 + GFF60 0.13 -0.9 50,786 - GFF60 0.14 +0.6 50,791 - GFF60 0.14 -0.3 50,791 - GFF60 0.14 +0.1 50,791 - GFF60 0.14 -1.4 50,798 - GFF60 0.14 -3.1 50,798 - GFF60 0.14 -0.1 50,812 - GFF60 0.15 +1.6 50,816 - GFF60 0.15 -0.2 50,821 - GFF60 0.15 +0.5 50,821 - GFF60 0.15 +0.3 50,821 - GFF60 0.15 +0.4 50,821 - GFF60 0.15 +0.3 50,821 - GFF60 0.15 -0.6 50,826 - GFF60 0.15 +1.1 50,860 - GFF60 0.16 -1.4 50,876 + GFF60 0.17 -2.2 50,964 - GFF60 0.20 -1.9 51,034 + GFF60 0.23 -1.5 51,034 + GFF60 0.23 -0.9 51,040 + GFF60 0.23 +0.4 51,073 + GFF60 0.24 -1.2 51,073 + GFF60 0.24 -0.3 51,081 - GFF60 0.24 +0.5 51,103 - GFF60 0.25 +0.0 51,128 + GFF60 0.26 -0.1 51,128 + GFF60 0.26 -0.2 51,136 - GFF60 0.27 +1.9 51,163 + GFF60 0.28 +0.1 51,179 + GFF60 0.28 +0.6 51,179 + GFF60 0.28 -0.9 51,191 + GFF60 0.29 -0.2 51,191 + GFF60 0.29 -0.4 51,205 + GFF60 0.29 +0.6 51,205 + GFF60 0.29 -0.5 51,205 + GFF60 0.29 -0.6 51,205 + GFF60 0.29 +0.3 51,230 + GFF60 0.30 -1.3 51,230 + GFF60 0.30 -0.4 51,230 + GFF60 0.30 -1.3 51,230 + GFF60 0.30 +0.6 51,230 + GFF60 0.30 -1.3 51,233 - GFF60 0.30 -2.2 51,238 - GFF60 0.30 +3.2 51,238 - GFF60 0.30 -0.3 51,238 - GFF60 0.30 -1.4 51,238 - GFF60 0.30 -0.4 51,278 + GFF60 0.32 -1.0 51,283 + GFF60 0.32 -0.1 51,316 + GFF60 0.33 +0.0 51,349 - GFF60 0.34 +1.0 51,409 - GFF60 0.37 -0.5 51,455 + GFF60 0.38 -0.0 51,463 - GFF60 0.39 +0.6 51,476 + GFF60 0.39 -1.0 51,484 - GFF60 0.39 +0.8 51,485 + GFF60 0.39 -0.9 51,490 + GFF60 0.40 +0.7 51,490 + GFF60 0.40 -1.3 51,498 - GFF60 0.40 -1.4 51,498 - GFF60 0.40 -0.3 51,498 - GFF60 0.40 +0.4 51,500 + GFF60 0.40 -0.6 51,500 + GFF60 0.40 +0.2 51,500 + GFF60 0.40 -1.9 51,500 + GFF60 0.40 -1.8 51,500 + GFF60 0.40 -0.2 51,500 + GFF60 0.40 -0.3 51,500 + GFF60 0.40 -0.5 51,500 + GFF60 0.40 -0.5 51,500 + GFF60 0.40 +0.4 51,500 + GFF60 0.40 +0.6 51,508 - GFF60 0.40 +0.0 51,508 - GFF60 0.40 -0.6 51,508 - GFF60 0.40 -1.3 51,508 - GFF60 0.40 +0.1 51,508 - GFF60 0.40 -1.3 51,508 - GFF60 0.40 -2.1 51,508 - GFF60 0.40 -1.6 51,508 - GFF60 0.40 -0.3 51,518 - GFF60 0.41 -0.8 51,523 - GFF60 0.41 +0.1 51,523 - GFF60 0.41 -1.0 51,525 + GFF60 0.41 -2.0 51,534 - GFF60 0.41 +0.1 51,538 + GFF60 0.41 -1.0 51,570 + GFF60 0.43 +0.4 51,574 + GFF60 0.43 +0.4 51,577 - GFF60 0.43 +0.1 51,577 - GFF60 0.43 -0.9 51,582 - GFF60 0.43 -0.6 51,598 - GFF60 0.44 +0.3 51,617 - GFF60 0.44 +1.0 51,636 - GFF60 0.45 -0.6 51,665 + GFF60 0.46 +0.1 51,677 + GFF60 0.47 +0.4 51,754 + GFF60 0.49 +0.7 51,754 + GFF60 0.49 +0.5 51,754 + GFF60 0.49 +1.1 51,767 + GFF60 0.50 -0.6 51,779 + GFF60 0.50 +1.3 51,818 + GFF60 0.52 -0.3 51,822 + GFF60 0.52 +0.1 51,831 - GFF60 0.52 -1.1 51,834 - GFF60 0.52 -0.6 51,834 - GFF60 0.52 -1.3 51,845 + GFF60 0.53 -0.3 51,883 + GFF60 0.54 -2.1 51,884 + GFF60 0.54 -0.3 51,884 + GFF60 0.54 +0.1 51,887 - GFF60 0.54 -1.1 51,891 - GFF60 0.54 +0.0 51,892 - GFF60 0.55 +1.3 51,938 + GFF60 0.56 +1.6 51,944 - GFF60 0.56 -0.2 51,964 + GFF60 0.57 -0.0 51,972 + GFF60 0.57 +0.6 51,977 + GFF60 0.58 +0.1 51,977 + GFF60 0.58 -1.0 51,977 + GFF60 0.58 -1.7 51,977 + GFF60 0.58 -0.4 51,985 - GFF60 0.58 -1.1 52,007 - GFF60 0.59 -0.8 52,015 - GFF60 0.59 +1.5 52,043 - GFF60 0.60 +0.1 52,069 + GFF60 0.61 -1.2 52,092 + GFF60 0.62 -0.3 52,092 - GFF60 0.62 +1.0 52,129 - GFF60 0.63 -0.6 52,131 + GFF60 0.63 +0.7 52,137 - GFF60 0.64 +0.8 52,137 - GFF60 0.64 -0.1 52,161 + GFF60 0.64 -0.8 52,161 + GFF60 0.64 +0.3 52,168 - GFF60 0.65 +1.9 52,171 + GFF60 0.65 -2.0 52,171 + GFF60 0.65 -1.0 52,171 + GFF60 0.65 -0.0 52,171 + GFF60 0.65 -1.2 52,171 + GFF60 0.65 -1.4 52,171 + GFF60 0.65 -0.3 52,171 + GFF60 0.65 -0.3 52,171 + GFF60 0.65 -0.7 52,171 + GFF60 0.65 -0.9 52,174 - GFF60 0.65 -2.2 52,174 - GFF60 0.65 +0.4 52,179 - GFF60 0.65 -0.4 52,179 - GFF60 0.65 -0.6 52,179 - GFF60 0.65 -1.3 52,179 - GFF60 0.65 -0.8 52,179 - GFF60 0.65 -0.8 52,181 + GFF60 0.65 -1.0 52,202 + GFF60 0.66 -2.1 52,209 + GFF60 0.66 -0.5 52,210 - GFF60 0.66 -2.3 52,217 - GFF60 0.67 -0.2 52,224 + GFF60 0.67 -0.9 52,224 + GFF60 0.67 -1.0 52,224 + GFF60 0.67 -0.3 52,282 - GFF60 0.69 +0.0 52,316 - GFF60 0.70 -0.9 52,320 + GFF60 0.70 +1.1 52,328 - GFF60 0.71 +0.3 52,338 + GFF60 0.71 -1.2 52,338 - GFF60 0.71 -0.8 52,338 - GFF60 0.71 +1.0 52,338 - GFF60 0.71 -1.2 52,338 - GFF60 0.71 -1.6 52,339 - GFF60 0.71 -0.3 52,346 - GFF60 0.71 -0.6 52,346 - GFF60 0.71 -0.6 52,348 + GFF60 0.71 -1.2 52,405 + GFF60 0.74 +1.8 52,405 + GFF60 0.74 +0.4 52,405 + GFF60 0.74 +0.4 52,418 + GFF60 0.74 -1.4 52,425 + GFF60 0.74 -2.2 52,425 + GFF60 0.74 -1.1 52,457 - GFF60 0.75 -1.3 52,483 - GFF60 0.76 -0.9 52,502 + GFF60 0.77 -0.2 52,510 - GFF60 0.77 -1.3 52,512 + GFF60 0.77 +0.2 52,546 + GFF60 0.79 -1.1 52,552 - GFF60 0.79 -1.2 52,554 - GFF60 0.79 -1.1 52,576 + GFF60 0.80 +0.3 52,601 + GFF60 0.81 -0.4 52,634 - GFF60 0.82 -1.4 52,642 + GFF60 0.82 +2.6 52,642 + GFF60 0.82 -0.1 52,750 - GFF60 0.86 +0.7 52,794 + GFF60 0.88 -0.1 52,794 - GFF60 0.88 -0.3 52,835 - GFF60 0.89 -0.4 52,914 + +0.7 52,914 + +2.2 52,927 - -0.1 52,986 + -1.3 53,062 - -1.2 53,064 + -1.1 53,139 + -0.4 53,164 + -0.4 53,164 + -0.1 53,164 + +2.0 53,164 + -1.0 53,195 + -0.8 53,244 - GFF61 0.16 +0.4 53,307 + GFF61 0.29 +1.4 53,414 - GFF61 0.50 -1.1 53,517 - GFF61 0.71 -0.5 53,605 + GFF61 0.89 -0.9 53,622 - -0.4 53,630 + +1.3 53,712 + +0.1 53,812 - GFF62 0.19 -0.1 53,907 + -0.3 53,981 + +0.4 53,988 + -1.0 53,988 + -0.3 53,988 + -1.8 53,996 - -0.1 54,010 + GFF63 0.17 +0.4 54,035 + GFF63 0.31 -0.3 54,043 - GFF63 0.36 +1.4 54,044 - GFF63 0.36 +0.2 54,075 + GFF63 0.53 +0.4 54,092 + GFF63 0.63 +0.6 54,105 + GFF63 0.70 +2.6 54,105 + GFF63 0.70 +0.4 54,113 - GFF63 0.74 -1.3 54,113 - GFF63 0.74 -1.1 54,119 + GFF63 0.78 +0.0
Or see this region's nucleotide sequence