Experiment: EMM_noCarbon with Sucrose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4 and GFF5 are separated by 2 nucleotides GFF5 and GFF6 are separated by 455 nucleotides GFF6 and GFF7 are separated by 157 nucleotides GFF7 and GFF8 are separated by 281 nucleotides
GFF4 - Cytotoxic translational repressor of toxin- antitoxin stability system, at 3,019 to 3,348
GFF4
GFF5 - DNA-binding protein, at 3,351 to 3,650
GFF5
GFF6 - hypothetical protein, at 4,106 to 4,231
GFF6
GFF7 - hypothetical protein, at 4,389 to 4,778
GFF7
GFF8 - hypothetical protein, at 5,060 to 5,461
GFF8
Position (kb) 4
5 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 3.165 kb on + strand, within GFF4 at 3.226 kb on - strand, within GFF4 at 3.226 kb on - strand, within GFF4 at 3.308 kb on - strand, within GFF4 at 3.308 kb on - strand, within GFF4 at 3.452 kb on + strand, within GFF5 at 3.452 kb on + strand, within GFF5 at 3.508 kb on + strand, within GFF5 at 3.508 kb on + strand, within GFF5 at 3.540 kb on + strand, within GFF5 at 3.540 kb on + strand, within GFF5 at 3.653 kb on - strand at 3.657 kb on - strand at 3.745 kb on - strand at 3.774 kb on - strand at 3.776 kb on + strand at 3.824 kb on + strand at 3.824 kb on + strand at 3.824 kb on + strand at 3.829 kb on + strand at 3.829 kb on + strand at 3.829 kb on + strand at 3.838 kb on - strand at 3.893 kb on + strand at 3.903 kb on + strand at 3.955 kb on + strand at 3.970 kb on + strand at 3.992 kb on - strand at 3.993 kb on - strand at 4.004 kb on + strand at 4.086 kb on - strand at 4.109 kb on + strand at 4.117 kb on - strand at 4.137 kb on + strand, within GFF6 at 4.153 kb on - strand, within GFF6 at 4.164 kb on - strand, within GFF6 at 4.178 kb on + strand, within GFF6 at 4.212 kb on - strand, within GFF6 at 4.217 kb on - strand, within GFF6 at 4.247 kb on - strand at 4.292 kb on + strand at 4.302 kb on - strand at 4.314 kb on + strand at 4.314 kb on + strand at 4.316 kb on + strand at 4.322 kb on - strand at 4.349 kb on - strand at 4.381 kb on + strand at 4.414 kb on + strand at 4.422 kb on - strand at 4.423 kb on - strand at 4.442 kb on + strand, within GFF7 at 4.521 kb on + strand, within GFF7 at 4.524 kb on - strand, within GFF7 at 4.554 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.596 kb on - strand, within GFF7 at 4.605 kb on - strand, within GFF7 at 4.647 kb on + strand, within GFF7 at 4.668 kb on + strand, within GFF7 at 4.668 kb on + strand, within GFF7 at 4.676 kb on - strand, within GFF7 at 4.678 kb on + strand, within GFF7 at 4.678 kb on + strand, within GFF7 at 4.686 kb on - strand, within GFF7 at 4.686 kb on - strand, within GFF7 at 4.686 kb on - strand, within GFF7 at 4.686 kb on - strand, within GFF7 at 4.696 kb on + strand, within GFF7 at 4.705 kb on + strand, within GFF7 at 4.717 kb on + strand, within GFF7 at 4.721 kb on + strand, within GFF7 at 4.737 kb on - strand, within GFF7 at 4.737 kb on - strand, within GFF7 at 4.739 kb on + strand, within GFF7 at 4.739 kb on + strand, within GFF7 at 4.742 kb on - strand at 4.747 kb on - strand at 4.747 kb on - strand at 4.747 kb on - strand at 4.757 kb on - strand at 4.801 kb on - strand at 4.858 kb on - strand at 4.888 kb on - strand at 5.080 kb on + strand at 5.151 kb on + strand, within GFF8 at 5.151 kb on + strand, within GFF8 at 5.151 kb on + strand, within GFF8 at 5.154 kb on - strand, within GFF8 at 5.176 kb on + strand, within GFF8 at 5.176 kb on + strand, within GFF8 at 5.210 kb on - strand, within GFF8 at 5.212 kb on + strand, within GFF8
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Ammonium Sulfate remove 3,165 + GFF4 0.44 +0.6 3,226 - GFF4 0.63 +0.1 3,226 - GFF4 0.63 +2.2 3,308 - GFF4 0.88 +0.6 3,308 - GFF4 0.88 -1.1 3,452 + GFF5 0.34 +1.3 3,452 + GFF5 0.34 +1.6 3,508 + GFF5 0.52 -0.0 3,508 + GFF5 0.52 -0.3 3,540 + GFF5 0.63 +0.0 3,540 + GFF5 0.63 -0.5 3,653 - +1.8 3,657 - -0.5 3,745 - -1.6 3,774 - -0.4 3,776 + -1.2 3,824 + +1.7 3,824 + -1.2 3,824 + -1.2 3,829 + +1.4 3,829 + +1.3 3,829 + +1.0 3,838 - -0.1 3,893 + -2.3 3,903 + +1.0 3,955 + +0.9 3,970 + -1.1 3,992 - -0.2 3,993 - -0.7 4,004 + -1.3 4,086 - -1.3 4,109 + -0.3 4,117 - -1.0 4,137 + GFF6 0.25 -2.4 4,153 - GFF6 0.37 +0.7 4,164 - GFF6 0.46 -0.8 4,178 + GFF6 0.57 -1.1 4,212 - GFF6 0.84 -2.4 4,217 - GFF6 0.88 +1.4 4,247 - +0.7 4,292 + -0.6 4,302 - -1.3 4,314 + +1.9 4,314 + -0.8 4,316 + -1.0 4,322 - +1.2 4,349 - -0.6 4,381 + +0.4 4,414 + -0.5 4,422 - -0.2 4,423 - +1.8 4,442 + GFF7 0.14 +0.4 4,521 + GFF7 0.34 -0.1 4,524 - GFF7 0.35 +1.6 4,554 + GFF7 0.42 -0.5 4,588 + GFF7 0.51 +0.7 4,588 + GFF7 0.51 +1.5 4,588 + GFF7 0.51 +0.4 4,588 + GFF7 0.51 -0.4 4,588 + GFF7 0.51 -0.7 4,588 + GFF7 0.51 +0.9 4,588 + GFF7 0.51 +0.5 4,588 + GFF7 0.51 -0.9 4,596 - GFF7 0.53 -0.9 4,605 - GFF7 0.55 +1.1 4,647 + GFF7 0.66 +1.0 4,668 + GFF7 0.72 -0.3 4,668 + GFF7 0.72 +1.8 4,676 - GFF7 0.74 -0.3 4,678 + GFF7 0.74 -0.6 4,678 + GFF7 0.74 +0.4 4,686 - GFF7 0.76 -1.0 4,686 - GFF7 0.76 -0.7 4,686 - GFF7 0.76 -0.0 4,686 - GFF7 0.76 -1.6 4,696 + GFF7 0.79 -0.1 4,705 + GFF7 0.81 +0.9 4,717 + GFF7 0.84 +0.6 4,721 + GFF7 0.85 -0.1 4,737 - GFF7 0.89 -0.5 4,737 - GFF7 0.89 -0.8 4,739 + GFF7 0.90 +1.1 4,739 + GFF7 0.90 +1.3 4,742 - +0.7 4,747 - +0.5 4,747 - -0.5 4,747 - -1.2 4,757 - -1.0 4,801 - +0.6 4,858 - -0.8 4,888 - +0.7 5,080 + +0.4 5,151 + GFF8 0.23 +0.3 5,151 + GFF8 0.23 +1.0 5,151 + GFF8 0.23 +1.0 5,154 - GFF8 0.23 -0.5 5,176 + GFF8 0.29 +3.0 5,176 + GFF8 0.29 +0.1 5,210 - GFF8 0.37 -0.2 5,212 + GFF8 0.38 -1.8
Or see this region's nucleotide sequence