Experiment: EMM_noCarbon with Sucrose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF487 and GFF488 are separated by 104 nucleotides GFF488 and GFF489 are separated by 165 nucleotides GFF489 and GFF490 are separated by 123 nucleotides GFF490 and GFF491 are separated by 94 nucleotides
GFF487 - Pyruvate kinase (EC 2.7.1.40), at 487,302 to 488,774
GFF487
GFF488 - protein of unknown function DUF1244, at 488,879 to 489,178
GFF488
GFF489 - hypothetical protein, at 489,344 to 489,586
GFF489
GFF490 - UPF0145 protein YbjQ, at 489,710 to 490,036
GFF490
GFF491 - Probable transcriptional regulatory protein YebC, at 490,131 to 490,874
GFF491
Position (kb) 488
489
490 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 487.881 kb on + strand, within GFF487 at 487.889 kb on - strand, within GFF487 at 487.907 kb on - strand, within GFF487 at 488.064 kb on - strand, within GFF487 at 488.078 kb on + strand, within GFF487 at 488.086 kb on - strand, within GFF487 at 488.095 kb on + strand, within GFF487 at 488.103 kb on - strand, within GFF487 at 488.103 kb on - strand, within GFF487 at 488.103 kb on - strand, within GFF487 at 488.110 kb on + strand, within GFF487 at 488.134 kb on + strand, within GFF487 at 488.177 kb on + strand, within GFF487 at 488.221 kb on + strand, within GFF487 at 488.231 kb on - strand, within GFF487 at 488.306 kb on + strand, within GFF487 at 488.306 kb on + strand, within GFF487 at 488.339 kb on + strand, within GFF487 at 488.429 kb on - strand, within GFF487 at 488.489 kb on - strand, within GFF487 at 488.530 kb on + strand, within GFF487 at 488.540 kb on + strand, within GFF487 at 488.540 kb on + strand, within GFF487 at 488.548 kb on - strand, within GFF487 at 488.566 kb on + strand, within GFF487 at 488.636 kb on - strand at 488.636 kb on - strand at 488.641 kb on + strand at 488.669 kb on - strand at 488.694 kb on + strand at 488.699 kb on + strand at 488.702 kb on - strand at 488.727 kb on - strand at 488.744 kb on - strand at 488.745 kb on - strand at 488.787 kb on - strand at 488.799 kb on + strand at 488.806 kb on - strand at 488.842 kb on + strand at 488.850 kb on - strand at 488.859 kb on - strand at 488.888 kb on + strand at 488.888 kb on + strand at 488.888 kb on + strand at 488.888 kb on + strand at 488.888 kb on + strand at 488.894 kb on - strand at 488.896 kb on - strand at 488.896 kb on - strand at 488.896 kb on - strand at 488.896 kb on - strand at 488.896 kb on - strand at 488.904 kb on - strand at 488.956 kb on - strand, within GFF488 at 488.980 kb on - strand, within GFF488 at 489.004 kb on + strand, within GFF488 at 489.013 kb on - strand, within GFF488 at 489.014 kb on - strand, within GFF488 at 489.026 kb on - strand, within GFF488 at 489.038 kb on + strand, within GFF488 at 489.038 kb on + strand, within GFF488 at 489.046 kb on - strand, within GFF488 at 489.050 kb on + strand, within GFF488 at 489.066 kb on - strand, within GFF488 at 489.074 kb on + strand, within GFF488 at 489.082 kb on - strand, within GFF488 at 489.082 kb on - strand, within GFF488 at 489.109 kb on + strand, within GFF488 at 489.109 kb on + strand, within GFF488 at 489.117 kb on - strand, within GFF488 at 489.117 kb on - strand, within GFF488 at 489.117 kb on - strand, within GFF488 at 489.117 kb on - strand, within GFF488 at 489.117 kb on - strand, within GFF488 at 489.122 kb on + strand, within GFF488 at 489.122 kb on + strand, within GFF488 at 489.122 kb on + strand, within GFF488 at 489.140 kb on + strand, within GFF488 at 489.140 kb on + strand, within GFF488 at 489.147 kb on - strand, within GFF488 at 489.148 kb on - strand, within GFF488 at 489.148 kb on - strand, within GFF488 at 489.148 kb on - strand, within GFF488 at 489.148 kb on - strand, within GFF488 at 489.148 kb on - strand, within GFF488 at 489.148 kb on - strand, within GFF488 at 489.158 kb on - strand at 489.171 kb on + strand at 489.172 kb on + strand at 489.172 kb on + strand at 489.175 kb on - strand at 489.179 kb on + strand at 489.187 kb on - strand at 489.187 kb on - strand at 489.187 kb on - strand at 489.197 kb on - strand at 489.202 kb on - strand at 489.207 kb on - strand at 489.237 kb on + strand at 489.257 kb on + strand at 489.267 kb on + strand at 489.272 kb on + strand at 489.576 kb on + strand at 489.576 kb on + strand at 489.581 kb on + strand at 489.581 kb on + strand at 489.581 kb on + strand at 489.589 kb on - strand at 489.589 kb on - strand at 489.589 kb on - strand at 489.589 kb on - strand at 489.589 kb on - strand at 489.603 kb on + strand at 489.610 kb on - strand at 489.613 kb on + strand at 489.613 kb on + strand at 489.613 kb on + strand at 489.613 kb on + strand at 489.613 kb on + strand at 489.613 kb on + strand at 489.613 kb on + strand at 489.613 kb on + strand at 489.613 kb on + strand at 489.613 kb on + strand at 489.620 kb on + strand at 489.620 kb on + strand at 489.621 kb on + strand at 489.621 kb on - strand at 489.621 kb on - strand at 489.621 kb on - strand at 489.629 kb on - strand at 489.637 kb on + strand at 489.643 kb on - strand at 489.648 kb on + strand at 489.649 kb on + strand at 489.654 kb on + strand at 489.659 kb on + strand at 489.667 kb on - strand at 489.667 kb on - strand at 489.677 kb on - strand at 489.706 kb on + strand at 489.717 kb on + strand at 489.725 kb on - strand at 489.739 kb on - strand at 489.743 kb on + strand, within GFF490 at 489.763 kb on + strand, within GFF490 at 489.763 kb on + strand, within GFF490 at 489.770 kb on - strand, within GFF490 at 489.771 kb on - strand, within GFF490 at 489.771 kb on - strand, within GFF490 at 489.799 kb on - strand, within GFF490 at 489.821 kb on + strand, within GFF490 at 489.821 kb on + strand, within GFF490 at 489.823 kb on + strand, within GFF490 at 489.823 kb on + strand, within GFF490 at 489.823 kb on + strand, within GFF490 at 489.829 kb on - strand, within GFF490 at 489.829 kb on - strand, within GFF490 at 489.831 kb on - strand, within GFF490 at 489.831 kb on - strand, within GFF490 at 489.831 kb on - strand, within GFF490 at 489.853 kb on - strand, within GFF490 at 489.864 kb on + strand, within GFF490 at 489.864 kb on + strand, within GFF490 at 489.867 kb on + strand, within GFF490 at 489.872 kb on - strand, within GFF490 at 489.872 kb on - strand, within GFF490 at 489.872 kb on - strand, within GFF490 at 489.883 kb on - strand, within GFF490 at 489.883 kb on - strand, within GFF490 at 489.931 kb on + strand, within GFF490 at 489.944 kb on + strand, within GFF490 at 489.944 kb on + strand, within GFF490 at 489.944 kb on - strand, within GFF490 at 489.952 kb on - strand, within GFF490 at 489.952 kb on - strand, within GFF490 at 489.959 kb on + strand, within GFF490 at 489.974 kb on - strand, within GFF490 at 489.974 kb on - strand, within GFF490 at 489.976 kb on + strand, within GFF490 at 489.979 kb on - strand, within GFF490 at 489.981 kb on + strand, within GFF490 at 489.998 kb on - strand, within GFF490 at 489.998 kb on - strand, within GFF490 at 490.018 kb on - strand at 490.027 kb on + strand at 490.043 kb on - strand at 490.055 kb on + strand at 490.055 kb on + strand at 490.055 kb on + strand at 490.055 kb on + strand at 490.055 kb on + strand at 490.061 kb on - strand at 490.063 kb on - strand at 490.063 kb on - strand at 490.063 kb on - strand at 490.063 kb on - strand at 490.064 kb on - strand at 490.072 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Ammonium Sulfate remove 487,881 + GFF487 0.39 -1.2 487,889 - GFF487 0.40 +0.2 487,907 - GFF487 0.41 +1.2 488,064 - GFF487 0.52 +0.5 488,078 + GFF487 0.53 +0.6 488,086 - GFF487 0.53 -0.8 488,095 + GFF487 0.54 -0.4 488,103 - GFF487 0.54 +1.6 488,103 - GFF487 0.54 -1.2 488,103 - GFF487 0.54 -0.8 488,110 + GFF487 0.55 +1.0 488,134 + GFF487 0.56 +0.6 488,177 + GFF487 0.59 -0.5 488,221 + GFF487 0.62 -0.7 488,231 - GFF487 0.63 -2.0 488,306 + GFF487 0.68 -0.7 488,306 + GFF487 0.68 -1.0 488,339 + GFF487 0.70 -1.5 488,429 - GFF487 0.77 -0.4 488,489 - GFF487 0.81 +0.5 488,530 + GFF487 0.83 +0.4 488,540 + GFF487 0.84 -0.4 488,540 + GFF487 0.84 +0.1 488,548 - GFF487 0.85 +0.2 488,566 + GFF487 0.86 -1.1 488,636 - -0.3 488,636 - +0.5 488,641 + -1.1 488,669 - +0.5 488,694 + -1.8 488,699 + -0.2 488,702 - +1.0 488,727 - -0.3 488,744 - -0.7 488,745 - +0.3 488,787 - -2.7 488,799 + -0.3 488,806 - -2.0 488,842 + -1.3 488,850 - -1.6 488,859 - -0.7 488,888 + -0.2 488,888 + -1.0 488,888 + -1.1 488,888 + -0.3 488,888 + +0.6 488,894 - -0.9 488,896 - -0.7 488,896 - -0.2 488,896 - -1.2 488,896 - -2.4 488,896 - -1.8 488,904 - +1.1 488,956 - GFF488 0.26 +0.5 488,980 - GFF488 0.34 -0.2 489,004 + GFF488 0.42 -0.8 489,013 - GFF488 0.45 -0.6 489,014 - GFF488 0.45 -0.9 489,026 - GFF488 0.49 -0.1 489,038 + GFF488 0.53 -1.0 489,038 + GFF488 0.53 -0.0 489,046 - GFF488 0.56 -3.7 489,050 + GFF488 0.57 -0.7 489,066 - GFF488 0.62 -2.3 489,074 + GFF488 0.65 -0.0 489,082 - GFF488 0.68 -0.5 489,082 - GFF488 0.68 -0.8 489,109 + GFF488 0.77 -0.8 489,109 + GFF488 0.77 -1.5 489,117 - GFF488 0.79 -1.8 489,117 - GFF488 0.79 -1.1 489,117 - GFF488 0.79 -0.6 489,117 - GFF488 0.79 -1.0 489,117 - GFF488 0.79 -1.5 489,122 + GFF488 0.81 -0.8 489,122 + GFF488 0.81 +0.8 489,122 + GFF488 0.81 -0.9 489,140 + GFF488 0.87 +0.5 489,140 + GFF488 0.87 -1.3 489,147 - GFF488 0.89 -0.9 489,148 - GFF488 0.90 -2.1 489,148 - GFF488 0.90 -1.6 489,148 - GFF488 0.90 -1.3 489,148 - GFF488 0.90 -1.7 489,148 - GFF488 0.90 +0.0 489,148 - GFF488 0.90 -2.5 489,158 - -0.1 489,171 + -1.7 489,172 + -0.2 489,172 + +0.3 489,175 - -2.6 489,179 + +0.6 489,187 - +1.0 489,187 - -1.5 489,187 - -0.2 489,197 - -0.1 489,202 - +1.3 489,207 - -1.4 489,237 + -1.3 489,257 + +0.2 489,267 + -0.9 489,272 + -2.2 489,576 + -0.7 489,576 + -1.4 489,581 + -0.8 489,581 + -1.0 489,581 + +0.2 489,589 - -1.5 489,589 - -1.1 489,589 - +0.2 489,589 - +1.2 489,589 - +1.3 489,603 + +1.2 489,610 - +1.4 489,613 + +1.1 489,613 + -2.7 489,613 + -0.9 489,613 + +0.5 489,613 + -0.2 489,613 + -1.6 489,613 + +0.8 489,613 + -0.4 489,613 + -1.8 489,613 + -1.4 489,620 + -0.8 489,620 + -0.2 489,621 + -1.2 489,621 - -0.8 489,621 - -0.3 489,621 - +1.0 489,629 - +0.6 489,637 + -1.5 489,643 - -0.3 489,648 + +1.0 489,649 + -2.0 489,654 + -0.7 489,659 + -1.0 489,667 - -1.2 489,667 - -0.1 489,677 - -1.3 489,706 + +1.2 489,717 + -0.3 489,725 - -0.8 489,739 - +0.7 489,743 + GFF490 0.10 -0.8 489,763 + GFF490 0.16 -1.7 489,763 + GFF490 0.16 -0.9 489,770 - GFF490 0.18 -1.1 489,771 - GFF490 0.19 +0.2 489,771 - GFF490 0.19 +0.2 489,799 - GFF490 0.27 -0.4 489,821 + GFF490 0.34 -0.9 489,821 + GFF490 0.34 -0.4 489,823 + GFF490 0.35 -0.1 489,823 + GFF490 0.35 -0.5 489,823 + GFF490 0.35 -1.7 489,829 - GFF490 0.36 +1.6 489,829 - GFF490 0.36 -1.4 489,831 - GFF490 0.37 -0.4 489,831 - GFF490 0.37 +0.3 489,831 - GFF490 0.37 -0.2 489,853 - GFF490 0.44 +0.1 489,864 + GFF490 0.47 -0.8 489,864 + GFF490 0.47 -1.9 489,867 + GFF490 0.48 -1.4 489,872 - GFF490 0.50 -0.7 489,872 - GFF490 0.50 +0.1 489,872 - GFF490 0.50 -0.7 489,883 - GFF490 0.53 +0.2 489,883 - GFF490 0.53 -2.3 489,931 + GFF490 0.68 -0.8 489,944 + GFF490 0.72 -1.2 489,944 + GFF490 0.72 -0.2 489,944 - GFF490 0.72 -0.9 489,952 - GFF490 0.74 +1.2 489,952 - GFF490 0.74 -1.0 489,959 + GFF490 0.76 -0.2 489,974 - GFF490 0.81 +0.1 489,974 - GFF490 0.81 -0.6 489,976 + GFF490 0.81 +1.2 489,979 - GFF490 0.82 -0.2 489,981 + GFF490 0.83 -1.4 489,998 - GFF490 0.88 -0.1 489,998 - GFF490 0.88 -1.0 490,018 - -0.9 490,027 + -3.0 490,043 - -1.7 490,055 + -1.4 490,055 + -0.0 490,055 + +1.0 490,055 + +0.9 490,055 + -0.1 490,061 - -0.9 490,063 - +0.1 490,063 - -1.4 490,063 - -0.4 490,063 - +0.2 490,064 - -1.7 490,072 - -0.6
Or see this region's nucleotide sequence