Strain Fitness in Sphingobium sp. HT1-2 around GFF352

Experiment: EMM_noCarbon with Sucrose and Ammonium Sulfate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF350 and GFF351 are separated by 1 nucleotidesGFF351 and GFF352 are separated by 9 nucleotidesGFF352 and GFF353 are separated by 2 nucleotidesGFF353 and GFF354 overlap by 1 nucleotides GFF350 - Capsular polysaccharide glycosyltransferase biosynthesis protein WcbB, at 342,840 to 344,135 GFF350 GFF351 - 'Bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) (EC 3.6.1.17)' transl_table=11, at 344,137 to 344,577 GFF351 GFF352 - Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (EC 2.8.3.5), at 344,587 to 345,222 GFF352 GFF353 - Acid phosphatase (EC 3.1.3.2), at 345,225 to 346,109 GFF353 GFF354 - Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5), at 346,109 to 346,819 GFF354 Position (kb) 344 345 346Strain fitness (log2 ratio) -2 -1 0 1 2 3at 343.688 kb on - strand, within GFF350at 343.695 kb on + strand, within GFF350at 343.741 kb on + strand, within GFF350at 343.743 kb on - strand, within GFF350at 343.743 kb on - strand, within GFF350at 343.817 kb on + strand, within GFF350at 343.825 kb on - strand, within GFF350at 343.825 kb on - strand, within GFF350at 343.902 kb on + strand, within GFF350at 343.977 kb on + strand, within GFF350at 343.989 kb on - strand, within GFF350at 344.005 kb on - strand, within GFF350at 344.008 kb on + strandat 344.039 kb on + strandat 344.039 kb on + strandat 344.135 kb on + strandat 344.135 kb on + strandat 344.135 kb on + strandat 344.135 kb on + strandat 344.135 kb on + strandat 344.135 kb on + strandat 344.143 kb on - strandat 344.143 kb on - strandat 344.143 kb on - strandat 344.143 kb on - strandat 344.143 kb on - strandat 344.143 kb on - strandat 344.143 kb on - strandat 344.143 kb on - strandat 344.143 kb on - strandat 344.219 kb on + strand, within GFF351at 344.234 kb on + strand, within GFF351at 344.257 kb on - strand, within GFF351at 344.291 kb on + strand, within GFF351at 344.291 kb on + strand, within GFF351at 344.291 kb on + strand, within GFF351at 344.291 kb on + strand, within GFF351at 344.291 kb on + strand, within GFF351at 344.291 kb on + strand, within GFF351at 344.291 kb on + strand, within GFF351at 344.296 kb on + strand, within GFF351at 344.299 kb on - strand, within GFF351at 344.299 kb on - strand, within GFF351at 344.299 kb on - strand, within GFF351at 344.299 kb on - strand, within GFF351at 344.299 kb on - strand, within GFF351at 344.299 kb on - strand, within GFF351at 344.299 kb on - strand, within GFF351at 344.305 kb on + strand, within GFF351at 344.323 kb on - strand, within GFF351at 344.331 kb on - strand, within GFF351at 344.372 kb on + strand, within GFF351at 344.415 kb on + strand, within GFF351at 344.439 kb on + strand, within GFF351at 344.491 kb on - strand, within GFF351at 344.500 kb on - strand, within GFF351at 344.504 kb on - strand, within GFF351at 344.524 kb on - strand, within GFF351at 344.524 kb on - strand, within GFF351at 344.524 kb on - strand, within GFF351at 344.579 kb on - strandat 344.583 kb on + strandat 344.633 kb on + strandat 344.639 kb on + strandat 344.643 kb on + strandat 344.657 kb on + strand, within GFF352at 344.660 kb on - strand, within GFF352at 344.693 kb on + strand, within GFF352at 344.700 kb on + strand, within GFF352at 344.708 kb on - strand, within GFF352at 344.708 kb on - strand, within GFF352at 344.747 kb on + strand, within GFF352at 344.755 kb on - strand, within GFF352at 344.791 kb on + strand, within GFF352at 344.797 kb on - strand, within GFF352at 344.799 kb on - strand, within GFF352at 344.801 kb on + strand, within GFF352at 344.824 kb on + strand, within GFF352at 344.825 kb on + strand, within GFF352at 344.842 kb on + strand, within GFF352at 344.843 kb on + strand, within GFF352at 344.849 kb on + strand, within GFF352at 344.849 kb on + strand, within GFF352at 344.849 kb on + strand, within GFF352at 344.850 kb on - strand, within GFF352at 344.894 kb on + strand, within GFF352at 344.912 kb on - strand, within GFF352at 344.950 kb on - strand, within GFF352at 344.960 kb on - strand, within GFF352at 344.997 kb on + strand, within GFF352at 345.017 kb on + strand, within GFF352at 345.025 kb on - strand, within GFF352at 345.025 kb on - strand, within GFF352at 345.025 kb on - strand, within GFF352at 345.030 kb on - strand, within GFF352at 345.030 kb on - strand, within GFF352at 345.030 kb on - strand, within GFF352at 345.035 kb on - strand, within GFF352at 345.045 kb on - strand, within GFF352at 345.052 kb on - strand, within GFF352at 345.107 kb on - strand, within GFF352at 345.107 kb on - strand, within GFF352at 345.107 kb on - strand, within GFF352at 345.212 kb on + strandat 345.219 kb on - strandat 345.219 kb on - strandat 345.220 kb on - strandat 345.291 kb on + strandat 345.313 kb on + strandat 345.313 kb on + strandat 345.327 kb on + strand, within GFF353at 345.345 kb on - strand, within GFF353at 345.348 kb on - strand, within GFF353at 345.348 kb on - strand, within GFF353at 345.363 kb on + strand, within GFF353at 345.388 kb on + strand, within GFF353at 345.393 kb on + strand, within GFF353at 345.393 kb on + strand, within GFF353at 345.403 kb on + strand, within GFF353at 345.403 kb on + strand, within GFF353at 345.408 kb on + strand, within GFF353at 345.411 kb on - strand, within GFF353at 345.411 kb on - strand, within GFF353at 345.411 kb on - strand, within GFF353at 345.455 kb on + strand, within GFF353at 345.463 kb on - strand, within GFF353at 345.463 kb on - strand, within GFF353at 345.473 kb on - strand, within GFF353at 345.530 kb on + strand, within GFF353at 345.552 kb on + strand, within GFF353at 345.555 kb on - strand, within GFF353at 345.560 kb on - strand, within GFF353at 345.560 kb on - strand, within GFF353at 345.608 kb on + strand, within GFF353at 345.621 kb on - strand, within GFF353at 345.636 kb on - strand, within GFF353at 345.650 kb on + strand, within GFF353at 345.663 kb on + strand, within GFF353at 345.682 kb on - strand, within GFF353at 345.704 kb on + strand, within GFF353at 345.728 kb on + strand, within GFF353at 345.730 kb on + strand, within GFF353at 345.737 kb on - strand, within GFF353at 345.774 kb on + strand, within GFF353at 345.774 kb on + strand, within GFF353at 345.777 kb on - strand, within GFF353at 345.779 kb on + strand, within GFF353at 345.782 kb on - strand, within GFF353at 345.857 kb on - strand, within GFF353at 345.870 kb on - strand, within GFF353at 345.883 kb on - strand, within GFF353at 345.939 kb on - strand, within GFF353at 345.939 kb on - strand, within GFF353at 346.034 kb on + strandat 346.059 kb on - strandat 346.132 kb on - strandat 346.142 kb on - strandat 346.186 kb on + strand, within GFF354at 346.210 kb on + strand, within GFF354

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Per-strain Table

Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Ammonium Sulfate
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343,688 - GFF350 0.65 -0.2
343,695 + GFF350 0.66 -0.3
343,741 + GFF350 0.70 +0.5
343,743 - GFF350 0.70 +0.2
343,743 - GFF350 0.70 -0.7
343,817 + GFF350 0.75 -1.8
343,825 - GFF350 0.76 +1.5
343,825 - GFF350 0.76 -0.8
343,902 + GFF350 0.82 -0.6
343,977 + GFF350 0.88 -0.7
343,989 - GFF350 0.89 +1.9
344,005 - GFF350 0.90 -0.7
344,008 + +1.2
344,039 + -0.2
344,039 + -1.3
344,135 + -0.5
344,135 + +0.1
344,135 + +0.3
344,135 + +1.2
344,135 + +0.5
344,135 + -0.8
344,143 - +1.5
344,143 - +0.3
344,143 - -0.4
344,143 - +0.7
344,143 - -1.2
344,143 - -1.3
344,143 - -0.7
344,143 - -1.6
344,143 - -0.4
344,219 + GFF351 0.19 +1.0
344,234 + GFF351 0.22 +0.3
344,257 - GFF351 0.27 -0.5
344,291 + GFF351 0.35 +0.0
344,291 + GFF351 0.35 +0.5
344,291 + GFF351 0.35 -0.4
344,291 + GFF351 0.35 -0.9
344,291 + GFF351 0.35 -0.8
344,291 + GFF351 0.35 +1.2
344,291 + GFF351 0.35 +0.3
344,296 + GFF351 0.36 +1.2
344,299 - GFF351 0.37 -0.1
344,299 - GFF351 0.37 -0.5
344,299 - GFF351 0.37 +0.6
344,299 - GFF351 0.37 -1.0
344,299 - GFF351 0.37 -0.3
344,299 - GFF351 0.37 -0.2
344,299 - GFF351 0.37 -0.1
344,305 + GFF351 0.38 -0.7
344,323 - GFF351 0.42 -1.3
344,331 - GFF351 0.44 -1.0
344,372 + GFF351 0.53 -0.1
344,415 + GFF351 0.63 -0.1
344,439 + GFF351 0.68 +0.1
344,491 - GFF351 0.80 +2.1
344,500 - GFF351 0.82 +0.5
344,504 - GFF351 0.83 +0.1
344,524 - GFF351 0.88 +2.5
344,524 - GFF351 0.88 -0.4
344,524 - GFF351 0.88 -0.1
344,579 - -0.1
344,583 + +0.5
344,633 + +0.3
344,639 + -1.2
344,643 + -0.4
344,657 + GFF352 0.11 +0.2
344,660 - GFF352 0.11 -0.5
344,693 + GFF352 0.17 -0.0
344,700 + GFF352 0.18 +0.6
344,708 - GFF352 0.19 -0.7
344,708 - GFF352 0.19 +0.3
344,747 + GFF352 0.25 +0.5
344,755 - GFF352 0.26 +0.1
344,791 + GFF352 0.32 +0.5
344,797 - GFF352 0.33 -1.0
344,799 - GFF352 0.33 -0.4
344,801 + GFF352 0.34 +0.2
344,824 + GFF352 0.37 +0.1
344,825 + GFF352 0.37 +0.3
344,842 + GFF352 0.40 -0.7
344,843 + GFF352 0.40 +0.7
344,849 + GFF352 0.41 +1.1
344,849 + GFF352 0.41 -0.5
344,849 + GFF352 0.41 -1.2
344,850 - GFF352 0.41 +1.3
344,894 + GFF352 0.48 +0.6
344,912 - GFF352 0.51 -0.3
344,950 - GFF352 0.57 +0.6
344,960 - GFF352 0.59 -0.2
344,997 + GFF352 0.64 -0.7
345,017 + GFF352 0.68 -0.7
345,025 - GFF352 0.69 -0.9
345,025 - GFF352 0.69 -0.5
345,025 - GFF352 0.69 -0.6
345,030 - GFF352 0.70 +0.3
345,030 - GFF352 0.70 -0.4
345,030 - GFF352 0.70 -1.8
345,035 - GFF352 0.70 +0.5
345,045 - GFF352 0.72 -0.7
345,052 - GFF352 0.73 -0.2
345,107 - GFF352 0.82 -0.4
345,107 - GFF352 0.82 -0.5
345,107 - GFF352 0.82 +0.3
345,212 + +0.7
345,219 - +0.1
345,219 - +0.7
345,220 - +0.3
345,291 + +1.2
345,313 + -0.1
345,313 + -0.9
345,327 + GFF353 0.12 -0.1
345,345 - GFF353 0.14 +0.3
345,348 - GFF353 0.14 -0.0
345,348 - GFF353 0.14 +0.3
345,363 + GFF353 0.16 -0.3
345,388 + GFF353 0.18 +0.4
345,393 + GFF353 0.19 +0.5
345,393 + GFF353 0.19 +0.8
345,403 + GFF353 0.20 +0.1
345,403 + GFF353 0.20 -1.6
345,408 + GFF353 0.21 -1.1
345,411 - GFF353 0.21 -0.2
345,411 - GFF353 0.21 +0.8
345,411 - GFF353 0.21 +0.9
345,455 + GFF353 0.26 +0.2
345,463 - GFF353 0.27 -0.8
345,463 - GFF353 0.27 -0.5
345,473 - GFF353 0.28 -0.1
345,530 + GFF353 0.34 -1.5
345,552 + GFF353 0.37 -0.4
345,555 - GFF353 0.37 -0.3
345,560 - GFF353 0.38 -0.1
345,560 - GFF353 0.38 -0.5
345,608 + GFF353 0.43 -0.2
345,621 - GFF353 0.45 -2.3
345,636 - GFF353 0.46 -0.7
345,650 + GFF353 0.48 -1.2
345,663 + GFF353 0.49 +1.5
345,682 - GFF353 0.52 -0.1
345,704 + GFF353 0.54 +1.1
345,728 + GFF353 0.57 +0.1
345,730 + GFF353 0.57 +0.1
345,737 - GFF353 0.58 -0.3
345,774 + GFF353 0.62 -1.3
345,774 + GFF353 0.62 -0.2
345,777 - GFF353 0.62 -1.2
345,779 + GFF353 0.63 +0.3
345,782 - GFF353 0.63 +1.3
345,857 - GFF353 0.71 +1.1
345,870 - GFF353 0.73 +0.1
345,883 - GFF353 0.74 +3.3
345,939 - GFF353 0.81 -0.4
345,939 - GFF353 0.81 -0.7
346,034 + +0.5
346,059 - -0.8
346,132 - +0.1
346,142 - -0.6
346,186 + GFF354 0.11 -1.4
346,210 + GFF354 0.14 +0.2

Or see this region's nucleotide sequence