Experiment: EMM_noCarbon with Sucrose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2 and GFF3 are separated by 215 nucleotides GFF3 and GFF4 are separated by 149 nucleotides GFF4 and GFF5 are separated by 2 nucleotides
GFF2 - hypothetical protein, at 1,182 to 1,940
GFF2
GFF3 - hypothetical protein, at 2,156 to 2,869
GFF3
GFF4 - Cytotoxic translational repressor of toxin- antitoxin stability system, at 3,019 to 3,348
GFF4
GFF5 - DNA-binding protein, at 3,351 to 3,650
GFF5
Position (kb)
2
3 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1.170 kb on - strand at 1.258 kb on - strand, within GFF2 at 1.341 kb on + strand, within GFF2 at 1.359 kb on + strand, within GFF2 at 1.359 kb on + strand, within GFF2 at 1.367 kb on - strand, within GFF2 at 1.368 kb on + strand, within GFF2 at 1.406 kb on + strand, within GFF2 at 1.406 kb on + strand, within GFF2 at 1.412 kb on - strand, within GFF2 at 1.414 kb on - strand, within GFF2 at 1.427 kb on - strand, within GFF2 at 1.438 kb on - strand, within GFF2 at 1.469 kb on - strand, within GFF2 at 1.495 kb on - strand, within GFF2 at 1.518 kb on - strand, within GFF2 at 1.611 kb on - strand, within GFF2 at 1.637 kb on - strand, within GFF2 at 1.656 kb on + strand, within GFF2 at 1.664 kb on - strand, within GFF2 at 1.664 kb on - strand, within GFF2 at 1.664 kb on - strand, within GFF2 at 1.748 kb on + strand, within GFF2 at 1.751 kb on - strand, within GFF2 at 1.751 kb on - strand, within GFF2 at 1.766 kb on - strand, within GFF2 at 1.786 kb on + strand, within GFF2 at 1.896 kb on + strand at 1.904 kb on - strand at 2.071 kb on + strand at 2.071 kb on + strand at 2.071 kb on + strand at 2.071 kb on + strand at 2.071 kb on + strand at 2.071 kb on + strand at 2.071 kb on + strand at 2.071 kb on + strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.081 kb on + strand at 2.088 kb on + strand at 2.096 kb on - strand at 2.113 kb on - strand at 2.174 kb on - strand at 2.238 kb on - strand, within GFF3 at 2.238 kb on - strand, within GFF3 at 2.296 kb on - strand, within GFF3 at 2.300 kb on + strand, within GFF3 at 2.301 kb on + strand, within GFF3 at 2.301 kb on + strand, within GFF3 at 2.301 kb on - strand, within GFF3 at 2.309 kb on - strand, within GFF3 at 2.332 kb on + strand, within GFF3 at 2.332 kb on + strand, within GFF3 at 2.335 kb on - strand, within GFF3 at 2.340 kb on - strand, within GFF3 at 2.347 kb on - strand, within GFF3 at 2.367 kb on + strand, within GFF3 at 2.367 kb on + strand, within GFF3 at 2.367 kb on + strand, within GFF3 at 2.367 kb on + strand, within GFF3 at 2.375 kb on - strand, within GFF3 at 2.377 kb on + strand, within GFF3 at 2.385 kb on - strand, within GFF3 at 2.390 kb on + strand, within GFF3 at 2.397 kb on - strand, within GFF3 at 2.411 kb on - strand, within GFF3 at 2.436 kb on + strand, within GFF3 at 2.466 kb on - strand, within GFF3 at 2.487 kb on - strand, within GFF3 at 2.492 kb on + strand, within GFF3 at 2.494 kb on + strand, within GFF3 at 2.502 kb on - strand, within GFF3 at 2.561 kb on - strand, within GFF3 at 2.563 kb on + strand, within GFF3 at 2.563 kb on + strand, within GFF3 at 2.563 kb on + strand, within GFF3 at 2.563 kb on + strand, within GFF3 at 2.563 kb on + strand, within GFF3 at 2.566 kb on - strand, within GFF3 at 2.571 kb on - strand, within GFF3 at 2.571 kb on - strand, within GFF3 at 2.571 kb on - strand, within GFF3 at 2.571 kb on - strand, within GFF3 at 2.606 kb on - strand, within GFF3 at 2.618 kb on + strand, within GFF3 at 2.618 kb on + strand, within GFF3 at 2.618 kb on + strand, within GFF3 at 2.618 kb on + strand, within GFF3 at 2.626 kb on + strand, within GFF3 at 2.626 kb on - strand, within GFF3 at 2.626 kb on - strand, within GFF3 at 2.626 kb on - strand, within GFF3 at 2.626 kb on - strand, within GFF3 at 2.627 kb on + strand, within GFF3 at 2.640 kb on + strand, within GFF3 at 2.654 kb on + strand, within GFF3 at 2.662 kb on + strand, within GFF3 at 2.662 kb on + strand, within GFF3 at 2.662 kb on + strand, within GFF3 at 2.670 kb on - strand, within GFF3 at 2.702 kb on + strand, within GFF3 at 2.744 kb on + strand, within GFF3 at 2.752 kb on - strand, within GFF3 at 2.758 kb on + strand, within GFF3 at 2.766 kb on - strand, within GFF3 at 2.783 kb on + strand, within GFF3 at 2.783 kb on + strand, within GFF3 at 2.783 kb on + strand, within GFF3 at 2.783 kb on + strand, within GFF3 at 2.783 kb on + strand, within GFF3 at 2.783 kb on + strand, within GFF3 at 2.791 kb on - strand, within GFF3 at 2.791 kb on - strand, within GFF3 at 2.801 kb on + strand at 2.894 kb on + strand at 2.902 kb on + strand at 2.931 kb on - strand at 2.931 kb on - strand at 2.937 kb on + strand at 3.017 kb on + strand at 3.017 kb on + strand at 3.038 kb on + strand at 3.040 kb on - strand at 3.043 kb on + strand at 3.043 kb on + strand at 3.043 kb on + strand at 3.043 kb on + strand at 3.043 kb on + strand at 3.043 kb on + strand at 3.043 kb on + strand at 3.046 kb on - strand at 3.051 kb on - strand at 3.077 kb on + strand, within GFF4 at 3.085 kb on - strand, within GFF4 at 3.090 kb on - strand, within GFF4 at 3.092 kb on + strand, within GFF4 at 3.165 kb on + strand, within GFF4 at 3.226 kb on - strand, within GFF4 at 3.226 kb on - strand, within GFF4 at 3.308 kb on - strand, within GFF4 at 3.308 kb on - strand, within GFF4 at 3.452 kb on + strand, within GFF5 at 3.452 kb on + strand, within GFF5 at 3.508 kb on + strand, within GFF5 at 3.508 kb on + strand, within GFF5 at 3.540 kb on + strand, within GFF5 at 3.540 kb on + strand, within GFF5 at 3.653 kb on - strand at 3.657 kb on - strand at 3.745 kb on - strand at 3.774 kb on - strand at 3.776 kb on + strand at 3.824 kb on + strand at 3.824 kb on + strand at 3.824 kb on + strand at 3.829 kb on + strand at 3.829 kb on + strand at 3.829 kb on + strand at 3.838 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Ammonium Sulfate remove 1,170 - +0.5 1,258 - GFF2 0.10 -0.7 1,341 + GFF2 0.21 -1.4 1,359 + GFF2 0.23 +0.9 1,359 + GFF2 0.23 +0.6 1,367 - GFF2 0.24 +1.7 1,368 + GFF2 0.25 +1.0 1,406 + GFF2 0.30 +0.5 1,406 + GFF2 0.30 +0.3 1,412 - GFF2 0.30 +0.5 1,414 - GFF2 0.31 -0.3 1,427 - GFF2 0.32 +1.0 1,438 - GFF2 0.34 -1.0 1,469 - GFF2 0.38 +0.5 1,495 - GFF2 0.41 -0.8 1,518 - GFF2 0.44 -1.4 1,611 - GFF2 0.57 +0.1 1,637 - GFF2 0.60 +0.7 1,656 + GFF2 0.62 +0.5 1,664 - GFF2 0.64 +0.8 1,664 - GFF2 0.64 +1.3 1,664 - GFF2 0.64 +1.3 1,748 + GFF2 0.75 -1.0 1,751 - GFF2 0.75 +1.8 1,751 - GFF2 0.75 +1.9 1,766 - GFF2 0.77 +0.5 1,786 + GFF2 0.80 +0.7 1,896 + -0.6 1,904 - +0.4 2,071 + -1.0 2,071 + -0.9 2,071 + +1.3 2,071 + +0.2 2,071 + -0.7 2,071 + +0.9 2,071 + -1.0 2,071 + -0.1 2,079 - +0.3 2,079 - -0.1 2,079 - -0.6 2,079 - -2.0 2,079 - -1.0 2,079 - -0.4 2,079 - -1.3 2,079 - -2.0 2,079 - -0.2 2,079 - -0.5 2,079 - -1.3 2,079 - +1.1 2,079 - +0.1 2,081 + -1.3 2,088 + +0.3 2,096 - +1.3 2,113 - +1.3 2,174 - +0.6 2,238 - GFF3 0.11 -1.8 2,238 - GFF3 0.11 +0.8 2,296 - GFF3 0.20 -1.8 2,300 + GFF3 0.20 +1.3 2,301 + GFF3 0.20 +1.7 2,301 + GFF3 0.20 -2.3 2,301 - GFF3 0.20 +1.4 2,309 - GFF3 0.21 +0.7 2,332 + GFF3 0.25 -0.0 2,332 + GFF3 0.25 -0.3 2,335 - GFF3 0.25 -0.5 2,340 - GFF3 0.26 -0.2 2,347 - GFF3 0.27 +0.3 2,367 + GFF3 0.30 +1.0 2,367 + GFF3 0.30 -0.2 2,367 + GFF3 0.30 -0.2 2,367 + GFF3 0.30 -0.7 2,375 - GFF3 0.31 +0.1 2,377 + GFF3 0.31 +0.1 2,385 - GFF3 0.32 +0.3 2,390 + GFF3 0.33 +0.5 2,397 - GFF3 0.34 +0.3 2,411 - GFF3 0.36 -1.2 2,436 + GFF3 0.39 +0.4 2,466 - GFF3 0.43 +1.4 2,487 - GFF3 0.46 -1.1 2,492 + GFF3 0.47 +0.5 2,494 + GFF3 0.47 -1.3 2,502 - GFF3 0.48 +0.3 2,561 - GFF3 0.57 +0.8 2,563 + GFF3 0.57 -0.3 2,563 + GFF3 0.57 +0.3 2,563 + GFF3 0.57 +2.3 2,563 + GFF3 0.57 -0.1 2,563 + GFF3 0.57 -1.7 2,566 - GFF3 0.57 +0.3 2,571 - GFF3 0.58 +0.4 2,571 - GFF3 0.58 -0.8 2,571 - GFF3 0.58 +0.4 2,571 - GFF3 0.58 +0.4 2,606 - GFF3 0.63 +0.6 2,618 + GFF3 0.65 -1.3 2,618 + GFF3 0.65 +0.7 2,618 + GFF3 0.65 +1.1 2,618 + GFF3 0.65 +0.5 2,626 + GFF3 0.66 -1.0 2,626 - GFF3 0.66 +1.2 2,626 - GFF3 0.66 -2.4 2,626 - GFF3 0.66 -0.4 2,626 - GFF3 0.66 +0.5 2,627 + GFF3 0.66 -0.3 2,640 + GFF3 0.68 -0.0 2,654 + GFF3 0.70 +0.9 2,662 + GFF3 0.71 -2.1 2,662 + GFF3 0.71 -1.6 2,662 + GFF3 0.71 +0.2 2,670 - GFF3 0.72 +0.2 2,702 + GFF3 0.76 -0.8 2,744 + GFF3 0.82 -1.0 2,752 - GFF3 0.83 +0.1 2,758 + GFF3 0.84 -1.1 2,766 - GFF3 0.85 +0.5 2,783 + GFF3 0.88 -0.2 2,783 + GFF3 0.88 +0.0 2,783 + GFF3 0.88 +0.2 2,783 + GFF3 0.88 -0.3 2,783 + GFF3 0.88 +0.0 2,783 + GFF3 0.88 -0.1 2,791 - GFF3 0.89 +1.6 2,791 - GFF3 0.89 +0.5 2,801 + -1.6 2,894 + -0.5 2,902 + -0.1 2,931 - -1.5 2,931 - -1.3 2,937 + +0.8 3,017 + -0.4 3,017 + +1.8 3,038 + +1.2 3,040 - -1.1 3,043 + -0.7 3,043 + -0.2 3,043 + -1.1 3,043 + +0.9 3,043 + -0.4 3,043 + -1.4 3,043 + +1.3 3,046 - -0.3 3,051 - +2.0 3,077 + GFF4 0.18 +0.2 3,085 - GFF4 0.20 -0.6 3,090 - GFF4 0.22 +2.7 3,092 + GFF4 0.22 -0.5 3,165 + GFF4 0.44 +0.6 3,226 - GFF4 0.63 +0.1 3,226 - GFF4 0.63 +2.2 3,308 - GFF4 0.88 +0.6 3,308 - GFF4 0.88 -1.1 3,452 + GFF5 0.34 +1.3 3,452 + GFF5 0.34 +1.6 3,508 + GFF5 0.52 -0.0 3,508 + GFF5 0.52 -0.3 3,540 + GFF5 0.63 +0.0 3,540 + GFF5 0.63 -0.5 3,653 - +1.8 3,657 - -0.5 3,745 - -1.6 3,774 - -0.4 3,776 + -1.2 3,824 + +1.7 3,824 + -1.2 3,824 + -1.2 3,829 + +1.4 3,829 + +1.3 3,829 + +1.0 3,838 - -0.1
Or see this region's nucleotide sequence