Experiment: EMM_noCarbon with Sucrose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1975 and GFF1976 overlap by 4 nucleotides GFF1976 and GFF1977 are separated by 49 nucleotides GFF1977 and GFF1978 are separated by 51 nucleotides
GFF1975 - Glycine/D-amino acid oxidases (deaminating), at 1,998,026 to 1,999,129
GFF1975
GFF1976 - lysophospholipase L2, at 1,999,126 to 2,000,079
GFF1976
GFF1977 - Type IV prepilin peptidase TadV/CpaA, at 2,000,129 to 2,000,602
GFF1977
GFF1978 - Flp pilus assembly protein RcpC/CpaB, at 2,000,654 to 2,001,667
GFF1978
Position (kb)
1999
2000
2001 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1998.135 kb on + strand at 1998.176 kb on + strand, within GFF1975 at 1998.197 kb on + strand, within GFF1975 at 1998.220 kb on + strand, within GFF1975 at 1998.230 kb on + strand, within GFF1975 at 1998.233 kb on - strand, within GFF1975 at 1998.233 kb on - strand, within GFF1975 at 1998.233 kb on - strand, within GFF1975 at 1998.235 kb on + strand, within GFF1975 at 1998.290 kb on - strand, within GFF1975 at 1998.292 kb on + strand, within GFF1975 at 1998.319 kb on - strand, within GFF1975 at 1998.327 kb on - strand, within GFF1975 at 1998.348 kb on - strand, within GFF1975 at 1998.374 kb on + strand, within GFF1975 at 1998.374 kb on + strand, within GFF1975 at 1998.420 kb on + strand, within GFF1975 at 1998.467 kb on + strand, within GFF1975 at 1998.467 kb on + strand, within GFF1975 at 1998.475 kb on - strand, within GFF1975 at 1998.475 kb on - strand, within GFF1975 at 1998.490 kb on + strand, within GFF1975 at 1998.513 kb on + strand, within GFF1975 at 1998.513 kb on + strand, within GFF1975 at 1998.513 kb on + strand, within GFF1975 at 1998.513 kb on - strand, within GFF1975 at 1998.513 kb on - strand, within GFF1975 at 1998.513 kb on - strand, within GFF1975 at 1998.521 kb on - strand, within GFF1975 at 1998.521 kb on - strand, within GFF1975 at 1998.521 kb on - strand, within GFF1975 at 1998.521 kb on - strand, within GFF1975 at 1998.521 kb on - strand, within GFF1975 at 1998.531 kb on - strand, within GFF1975 at 1998.545 kb on - strand, within GFF1975 at 1998.557 kb on + strand, within GFF1975 at 1998.560 kb on - strand, within GFF1975 at 1998.588 kb on - strand, within GFF1975 at 1998.588 kb on - strand, within GFF1975 at 1998.588 kb on - strand, within GFF1975 at 1998.611 kb on + strand, within GFF1975 at 1998.611 kb on + strand, within GFF1975 at 1998.619 kb on - strand, within GFF1975 at 1998.619 kb on - strand, within GFF1975 at 1998.624 kb on - strand, within GFF1975 at 1998.628 kb on + strand, within GFF1975 at 1998.636 kb on - strand, within GFF1975 at 1998.647 kb on - strand, within GFF1975 at 1998.647 kb on - strand, within GFF1975 at 1998.681 kb on + strand, within GFF1975 at 1998.682 kb on + strand, within GFF1975 at 1998.687 kb on + strand, within GFF1975 at 1998.694 kb on - strand, within GFF1975 at 1998.695 kb on - strand, within GFF1975 at 1998.700 kb on + strand, within GFF1975 at 1998.706 kb on + strand, within GFF1975 at 1998.715 kb on + strand, within GFF1975 at 1998.737 kb on + strand, within GFF1975 at 1998.738 kb on + strand, within GFF1975 at 1998.740 kb on - strand, within GFF1975 at 1998.742 kb on + strand, within GFF1975 at 1998.745 kb on - strand, within GFF1975 at 1998.746 kb on - strand, within GFF1975 at 1998.749 kb on + strand, within GFF1975 at 1998.750 kb on - strand, within GFF1975 at 1998.761 kb on + strand, within GFF1975 at 1998.769 kb on - strand, within GFF1975 at 1998.769 kb on - strand, within GFF1975 at 1998.771 kb on + strand, within GFF1975 at 1998.774 kb on - strand, within GFF1975 at 1998.779 kb on - strand, within GFF1975 at 1998.823 kb on + strand, within GFF1975 at 1998.831 kb on + strand, within GFF1975 at 1998.841 kb on - strand, within GFF1975 at 1998.847 kb on - strand, within GFF1975 at 1998.928 kb on - strand, within GFF1975 at 1999.014 kb on - strand, within GFF1975 at 1999.015 kb on + strand, within GFF1975 at 1999.033 kb on - strand at 1999.048 kb on - strand at 1999.078 kb on + strand at 1999.136 kb on + strand at 1999.136 kb on + strand at 1999.144 kb on - strand at 1999.157 kb on + strand at 1999.178 kb on - strand at 1999.208 kb on - strand at 1999.241 kb on + strand, within GFF1976 at 1999.244 kb on - strand, within GFF1976 at 1999.259 kb on - strand, within GFF1976 at 1999.307 kb on + strand, within GFF1976 at 1999.307 kb on + strand, within GFF1976 at 1999.307 kb on + strand, within GFF1976 at 1999.307 kb on + strand, within GFF1976 at 1999.311 kb on + strand, within GFF1976 at 1999.315 kb on - strand, within GFF1976 at 1999.360 kb on + strand, within GFF1976 at 1999.375 kb on - strand, within GFF1976 at 1999.478 kb on + strand, within GFF1976 at 1999.517 kb on + strand, within GFF1976 at 1999.517 kb on - strand, within GFF1976 at 1999.523 kb on - strand, within GFF1976 at 1999.526 kb on + strand, within GFF1976 at 1999.526 kb on + strand, within GFF1976 at 1999.534 kb on - strand, within GFF1976 at 1999.534 kb on - strand, within GFF1976 at 1999.534 kb on - strand, within GFF1976 at 1999.561 kb on + strand, within GFF1976 at 1999.563 kb on + strand, within GFF1976 at 1999.569 kb on - strand, within GFF1976 at 1999.604 kb on + strand, within GFF1976 at 1999.612 kb on - strand, within GFF1976 at 1999.655 kb on + strand, within GFF1976 at 1999.683 kb on + strand, within GFF1976 at 1999.683 kb on + strand, within GFF1976 at 1999.691 kb on - strand, within GFF1976 at 1999.691 kb on - strand, within GFF1976 at 1999.691 kb on - strand, within GFF1976 at 1999.691 kb on - strand, within GFF1976 at 1999.694 kb on + strand, within GFF1976 at 1999.694 kb on + strand, within GFF1976 at 1999.700 kb on - strand, within GFF1976 at 1999.716 kb on + strand, within GFF1976 at 1999.716 kb on + strand, within GFF1976 at 1999.724 kb on + strand, within GFF1976 at 1999.724 kb on - strand, within GFF1976 at 1999.729 kb on - strand, within GFF1976 at 1999.734 kb on - strand, within GFF1976 at 1999.821 kb on + strand, within GFF1976 at 1999.837 kb on - strand, within GFF1976 at 1999.847 kb on - strand, within GFF1976 at 1999.856 kb on - strand, within GFF1976 at 1999.856 kb on - strand, within GFF1976 at 1999.856 kb on - strand, within GFF1976 at 1999.865 kb on + strand, within GFF1976 at 1999.866 kb on - strand, within GFF1976 at 1999.873 kb on - strand, within GFF1976 at 1999.873 kb on - strand, within GFF1976 at 1999.873 kb on - strand, within GFF1976 at 1999.873 kb on - strand, within GFF1976 at 1999.878 kb on - strand, within GFF1976 at 1999.878 kb on - strand, within GFF1976 at 1999.931 kb on + strand, within GFF1976 at 1999.969 kb on - strand, within GFF1976 at 1999.988 kb on - strand at 1999.992 kb on + strand at 2000.032 kb on - strand at 2000.043 kb on + strand at 2000.043 kb on + strand at 2000.043 kb on + strand at 2000.051 kb on - strand at 2000.089 kb on - strand at 2000.103 kb on + strand at 2000.117 kb on + strand at 2000.126 kb on - strand at 2000.130 kb on + strand at 2000.132 kb on - strand at 2000.160 kb on + strand at 2000.172 kb on + strand at 2000.190 kb on - strand, within GFF1977 at 2000.196 kb on + strand, within GFF1977 at 2000.204 kb on - strand, within GFF1977 at 2000.223 kb on - strand, within GFF1977 at 2000.223 kb on - strand, within GFF1977 at 2000.241 kb on - strand, within GFF1977 at 2000.258 kb on + strand, within GFF1977 at 2000.263 kb on + strand, within GFF1977 at 2000.271 kb on - strand, within GFF1977 at 2000.287 kb on + strand, within GFF1977 at 2000.292 kb on + strand, within GFF1977 at 2000.292 kb on + strand, within GFF1977 at 2000.296 kb on + strand, within GFF1977 at 2000.296 kb on + strand, within GFF1977 at 2000.303 kb on + strand, within GFF1977 at 2000.311 kb on - strand, within GFF1977 at 2000.322 kb on + strand, within GFF1977 at 2000.324 kb on + strand, within GFF1977 at 2000.331 kb on + strand, within GFF1977 at 2000.339 kb on - strand, within GFF1977 at 2000.345 kb on + strand, within GFF1977 at 2000.390 kb on - strand, within GFF1977 at 2000.429 kb on + strand, within GFF1977 at 2000.429 kb on + strand, within GFF1977 at 2000.432 kb on - strand, within GFF1977 at 2000.437 kb on - strand, within GFF1977 at 2000.437 kb on - strand, within GFF1977 at 2000.437 kb on - strand, within GFF1977 at 2000.448 kb on - strand, within GFF1977 at 2000.474 kb on - strand, within GFF1977 at 2000.513 kb on - strand, within GFF1977 at 2000.518 kb on - strand, within GFF1977 at 2000.531 kb on - strand, within GFF1977 at 2000.613 kb on + strand at 2000.613 kb on + strand at 2000.613 kb on + strand at 2000.613 kb on + strand at 2000.677 kb on + strand at 2000.685 kb on - strand at 2000.694 kb on + strand at 2000.706 kb on + strand at 2000.706 kb on + strand at 2000.706 kb on + strand at 2000.758 kb on - strand, within GFF1978 at 2000.758 kb on - strand, within GFF1978 at 2000.794 kb on - strand, within GFF1978 at 2000.817 kb on - strand, within GFF1978 at 2000.829 kb on + strand, within GFF1978 at 2000.834 kb on + strand, within GFF1978 at 2000.837 kb on - strand, within GFF1978 at 2000.837 kb on - strand, within GFF1978 at 2000.871 kb on - strand, within GFF1978 at 2000.876 kb on + strand, within GFF1978 at 2000.876 kb on + strand, within GFF1978 at 2000.889 kb on - strand, within GFF1978 at 2000.889 kb on - strand, within GFF1978 at 2000.906 kb on + strand, within GFF1978 at 2000.925 kb on + strand, within GFF1978 at 2000.933 kb on - strand, within GFF1978 at 2000.937 kb on + strand, within GFF1978 at 2000.974 kb on + strand, within GFF1978 at 2000.994 kb on + strand, within GFF1978 at 2001.023 kb on + strand, within GFF1978 at 2001.030 kb on + strand, within GFF1978 at 2001.058 kb on + strand, within GFF1978
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Ammonium Sulfate remove 1,998,135 + -1.3 1,998,176 + GFF1975 0.14 -0.1 1,998,197 + GFF1975 0.15 +0.3 1,998,220 + GFF1975 0.18 -1.9 1,998,230 + GFF1975 0.18 -0.3 1,998,233 - GFF1975 0.19 +1.0 1,998,233 - GFF1975 0.19 +1.0 1,998,233 - GFF1975 0.19 -0.9 1,998,235 + GFF1975 0.19 +0.4 1,998,290 - GFF1975 0.24 -0.7 1,998,292 + GFF1975 0.24 +1.3 1,998,319 - GFF1975 0.27 -1.2 1,998,327 - GFF1975 0.27 -0.7 1,998,348 - GFF1975 0.29 -0.2 1,998,374 + GFF1975 0.32 -1.2 1,998,374 + GFF1975 0.32 +0.3 1,998,420 + GFF1975 0.36 -1.0 1,998,467 + GFF1975 0.40 -1.1 1,998,467 + GFF1975 0.40 +0.3 1,998,475 - GFF1975 0.41 -0.4 1,998,475 - GFF1975 0.41 +0.3 1,998,490 + GFF1975 0.42 +0.7 1,998,513 + GFF1975 0.44 +1.1 1,998,513 + GFF1975 0.44 -0.8 1,998,513 + GFF1975 0.44 +0.6 1,998,513 - GFF1975 0.44 -0.9 1,998,513 - GFF1975 0.44 +1.1 1,998,513 - GFF1975 0.44 -2.1 1,998,521 - GFF1975 0.45 +0.7 1,998,521 - GFF1975 0.45 -1.3 1,998,521 - GFF1975 0.45 -1.7 1,998,521 - GFF1975 0.45 -0.4 1,998,521 - GFF1975 0.45 -0.3 1,998,531 - GFF1975 0.46 +0.1 1,998,545 - GFF1975 0.47 -0.6 1,998,557 + GFF1975 0.48 -0.4 1,998,560 - GFF1975 0.48 +0.6 1,998,588 - GFF1975 0.51 -0.4 1,998,588 - GFF1975 0.51 -0.9 1,998,588 - GFF1975 0.51 +2.3 1,998,611 + GFF1975 0.53 -1.6 1,998,611 + GFF1975 0.53 -0.6 1,998,619 - GFF1975 0.54 +0.1 1,998,619 - GFF1975 0.54 +0.9 1,998,624 - GFF1975 0.54 +0.4 1,998,628 + GFF1975 0.55 -0.8 1,998,636 - GFF1975 0.55 +0.4 1,998,647 - GFF1975 0.56 -0.1 1,998,647 - GFF1975 0.56 +0.9 1,998,681 + GFF1975 0.59 +0.2 1,998,682 + GFF1975 0.59 +0.8 1,998,687 + GFF1975 0.60 +0.9 1,998,694 - GFF1975 0.61 -0.6 1,998,695 - GFF1975 0.61 +0.2 1,998,700 + GFF1975 0.61 +0.8 1,998,706 + GFF1975 0.62 -0.1 1,998,715 + GFF1975 0.62 +0.5 1,998,737 + GFF1975 0.64 -0.8 1,998,738 + GFF1975 0.64 +0.2 1,998,740 - GFF1975 0.65 -0.2 1,998,742 + GFF1975 0.65 -0.1 1,998,745 - GFF1975 0.65 +0.9 1,998,746 - GFF1975 0.65 +0.7 1,998,749 + GFF1975 0.65 -1.1 1,998,750 - GFF1975 0.66 -1.2 1,998,761 + GFF1975 0.67 -0.4 1,998,769 - GFF1975 0.67 +1.0 1,998,769 - GFF1975 0.67 -1.3 1,998,771 + GFF1975 0.67 +0.6 1,998,774 - GFF1975 0.68 +2.9 1,998,779 - GFF1975 0.68 +0.2 1,998,823 + GFF1975 0.72 -0.2 1,998,831 + GFF1975 0.73 -1.2 1,998,841 - GFF1975 0.74 -1.0 1,998,847 - GFF1975 0.74 -0.9 1,998,928 - GFF1975 0.82 -1.0 1,999,014 - GFF1975 0.89 -0.2 1,999,015 + GFF1975 0.90 -0.1 1,999,033 - +0.3 1,999,048 - -1.5 1,999,078 + -1.6 1,999,136 + -0.0 1,999,136 + +0.9 1,999,144 - -0.1 1,999,157 + +0.8 1,999,178 - +0.7 1,999,208 - +0.2 1,999,241 + GFF1976 0.12 -0.7 1,999,244 - GFF1976 0.12 -0.8 1,999,259 - GFF1976 0.14 +0.3 1,999,307 + GFF1976 0.19 +0.9 1,999,307 + GFF1976 0.19 +1.0 1,999,307 + GFF1976 0.19 +1.5 1,999,307 + GFF1976 0.19 +0.6 1,999,311 + GFF1976 0.19 +1.3 1,999,315 - GFF1976 0.20 -1.2 1,999,360 + GFF1976 0.25 +1.6 1,999,375 - GFF1976 0.26 +0.3 1,999,478 + GFF1976 0.37 +0.4 1,999,517 + GFF1976 0.41 +0.9 1,999,517 - GFF1976 0.41 +0.3 1,999,523 - GFF1976 0.42 +1.2 1,999,526 + GFF1976 0.42 +1.1 1,999,526 + GFF1976 0.42 -0.6 1,999,534 - GFF1976 0.43 +0.3 1,999,534 - GFF1976 0.43 -0.1 1,999,534 - GFF1976 0.43 -0.6 1,999,561 + GFF1976 0.46 -0.9 1,999,563 + GFF1976 0.46 -0.0 1,999,569 - GFF1976 0.46 +2.1 1,999,604 + GFF1976 0.50 -0.7 1,999,612 - GFF1976 0.51 +0.3 1,999,655 + GFF1976 0.55 -1.3 1,999,683 + GFF1976 0.58 +1.4 1,999,683 + GFF1976 0.58 -0.2 1,999,691 - GFF1976 0.59 +0.2 1,999,691 - GFF1976 0.59 -0.2 1,999,691 - GFF1976 0.59 +0.7 1,999,691 - GFF1976 0.59 +1.5 1,999,694 + GFF1976 0.60 +1.0 1,999,694 + GFF1976 0.60 +0.8 1,999,700 - GFF1976 0.60 -0.7 1,999,716 + GFF1976 0.62 -0.5 1,999,716 + GFF1976 0.62 -0.7 1,999,724 + GFF1976 0.63 +0.6 1,999,724 - GFF1976 0.63 +0.3 1,999,729 - GFF1976 0.63 -0.6 1,999,734 - GFF1976 0.64 +0.6 1,999,821 + GFF1976 0.73 -1.1 1,999,837 - GFF1976 0.75 +1.8 1,999,847 - GFF1976 0.76 -1.2 1,999,856 - GFF1976 0.77 -1.1 1,999,856 - GFF1976 0.77 -0.6 1,999,856 - GFF1976 0.77 -0.8 1,999,865 + GFF1976 0.77 -1.7 1,999,866 - GFF1976 0.78 -0.1 1,999,873 - GFF1976 0.78 +0.4 1,999,873 - GFF1976 0.78 -0.1 1,999,873 - GFF1976 0.78 -0.8 1,999,873 - GFF1976 0.78 +1.3 1,999,878 - GFF1976 0.79 +0.2 1,999,878 - GFF1976 0.79 +1.9 1,999,931 + GFF1976 0.84 +1.3 1,999,969 - GFF1976 0.88 +2.1 1,999,988 - -0.0 1,999,992 + +2.1 2,000,032 - -2.2 2,000,043 + -0.8 2,000,043 + +0.5 2,000,043 + +1.3 2,000,051 - +0.1 2,000,089 - +1.9 2,000,103 + +1.2 2,000,117 + +0.1 2,000,126 - -0.6 2,000,130 + -2.2 2,000,132 - +1.0 2,000,160 + +1.5 2,000,172 + -1.1 2,000,190 - GFF1977 0.13 +1.3 2,000,196 + GFF1977 0.14 -0.4 2,000,204 - GFF1977 0.16 +0.5 2,000,223 - GFF1977 0.20 -0.8 2,000,223 - GFF1977 0.20 +1.5 2,000,241 - GFF1977 0.24 +0.3 2,000,258 + GFF1977 0.27 -0.0 2,000,263 + GFF1977 0.28 +0.3 2,000,271 - GFF1977 0.30 -1.2 2,000,287 + GFF1977 0.33 -0.9 2,000,292 + GFF1977 0.34 -1.0 2,000,292 + GFF1977 0.34 +0.1 2,000,296 + GFF1977 0.35 -0.1 2,000,296 + GFF1977 0.35 +0.4 2,000,303 + GFF1977 0.37 -0.8 2,000,311 - GFF1977 0.38 -1.9 2,000,322 + GFF1977 0.41 -0.1 2,000,324 + GFF1977 0.41 -0.7 2,000,331 + GFF1977 0.43 +1.2 2,000,339 - GFF1977 0.44 -1.1 2,000,345 + GFF1977 0.46 +0.3 2,000,390 - GFF1977 0.55 +1.0 2,000,429 + GFF1977 0.63 -0.9 2,000,429 + GFF1977 0.63 -0.5 2,000,432 - GFF1977 0.64 -0.9 2,000,437 - GFF1977 0.65 +1.2 2,000,437 - GFF1977 0.65 -1.0 2,000,437 - GFF1977 0.65 -1.2 2,000,448 - GFF1977 0.67 +0.9 2,000,474 - GFF1977 0.73 +0.2 2,000,513 - GFF1977 0.81 -1.0 2,000,518 - GFF1977 0.82 +0.9 2,000,531 - GFF1977 0.85 -0.5 2,000,613 + +0.7 2,000,613 + +0.9 2,000,613 + +0.2 2,000,613 + +0.0 2,000,677 + +1.3 2,000,685 - +0.2 2,000,694 + -1.1 2,000,706 + +1.0 2,000,706 + -0.6 2,000,706 + +0.9 2,000,758 - GFF1978 0.10 -1.3 2,000,758 - GFF1978 0.10 +0.0 2,000,794 - GFF1978 0.14 +1.7 2,000,817 - GFF1978 0.16 -1.6 2,000,829 + GFF1978 0.17 +0.7 2,000,834 + GFF1978 0.18 +0.3 2,000,837 - GFF1978 0.18 -1.6 2,000,837 - GFF1978 0.18 -0.1 2,000,871 - GFF1978 0.21 -2.0 2,000,876 + GFF1978 0.22 -0.9 2,000,876 + GFF1978 0.22 +0.5 2,000,889 - GFF1978 0.23 +1.0 2,000,889 - GFF1978 0.23 -0.2 2,000,906 + GFF1978 0.25 -0.3 2,000,925 + GFF1978 0.27 +0.3 2,000,933 - GFF1978 0.28 -0.7 2,000,937 + GFF1978 0.28 +0.3 2,000,974 + GFF1978 0.32 +0.6 2,000,994 + GFF1978 0.34 +2.3 2,001,023 + GFF1978 0.36 +0.3 2,001,030 + GFF1978 0.37 -0.3 2,001,058 + GFF1978 0.40 -0.4
Or see this region's nucleotide sequence