Experiment: EMM_noCarbon with Sucrose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF113 and GFF114 are separated by 21 nucleotides GFF114 and GFF115 are separated by 134 nucleotides
GFF113 - Rossmann fold nucleotide-binding protein Smf possibly involved in DNA uptake, at 104,039 to 105,121
GFF113
GFF114 - hypothetical protein, at 105,143 to 106,021
GFF114
GFF115 - DNA topoisomerase I (EC 5.99.1.2), at 106,156 to 108,738
GFF115
Position (kb)
105
106
107 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 104.163 kb on + strand, within GFF113 at 104.236 kb on - strand, within GFF113 at 104.280 kb on + strand, within GFF113 at 104.283 kb on - strand, within GFF113 at 104.288 kb on - strand, within GFF113 at 104.288 kb on - strand, within GFF113 at 104.288 kb on - strand, within GFF113 at 104.288 kb on - strand, within GFF113 at 104.288 kb on - strand, within GFF113 at 104.307 kb on - strand, within GFF113 at 104.307 kb on - strand, within GFF113 at 104.323 kb on + strand, within GFF113 at 104.342 kb on - strand, within GFF113 at 104.356 kb on + strand, within GFF113 at 104.356 kb on + strand, within GFF113 at 104.356 kb on + strand, within GFF113 at 104.361 kb on + strand, within GFF113 at 104.416 kb on + strand, within GFF113 at 104.435 kb on + strand, within GFF113 at 104.453 kb on + strand, within GFF113 at 104.455 kb on - strand, within GFF113 at 104.461 kb on - strand, within GFF113 at 104.465 kb on - strand, within GFF113 at 104.478 kb on - strand, within GFF113 at 104.531 kb on - strand, within GFF113 at 104.583 kb on + strand, within GFF113 at 104.583 kb on + strand, within GFF113 at 104.583 kb on + strand, within GFF113 at 104.591 kb on - strand, within GFF113 at 104.591 kb on - strand, within GFF113 at 104.598 kb on + strand, within GFF113 at 104.598 kb on + strand, within GFF113 at 104.602 kb on + strand, within GFF113 at 104.606 kb on - strand, within GFF113 at 104.625 kb on + strand, within GFF113 at 104.633 kb on - strand, within GFF113 at 104.633 kb on - strand, within GFF113 at 104.652 kb on - strand, within GFF113 at 104.673 kb on - strand, within GFF113 at 104.676 kb on + strand, within GFF113 at 104.686 kb on + strand, within GFF113 at 104.686 kb on + strand, within GFF113 at 104.688 kb on + strand, within GFF113 at 104.693 kb on + strand, within GFF113 at 104.694 kb on - strand, within GFF113 at 104.694 kb on - strand, within GFF113 at 104.701 kb on - strand, within GFF113 at 104.733 kb on + strand, within GFF113 at 104.733 kb on + strand, within GFF113 at 104.733 kb on + strand, within GFF113 at 104.736 kb on - strand, within GFF113 at 104.740 kb on + strand, within GFF113 at 104.741 kb on - strand, within GFF113 at 104.741 kb on - strand, within GFF113 at 104.741 kb on - strand, within GFF113 at 104.748 kb on - strand, within GFF113 at 104.764 kb on + strand, within GFF113 at 104.782 kb on - strand, within GFF113 at 104.806 kb on + strand, within GFF113 at 104.806 kb on + strand, within GFF113 at 104.814 kb on - strand, within GFF113 at 104.816 kb on + strand, within GFF113 at 104.824 kb on - strand, within GFF113 at 104.848 kb on - strand, within GFF113 at 104.851 kb on - strand, within GFF113 at 104.852 kb on + strand, within GFF113 at 104.880 kb on + strand, within GFF113 at 104.880 kb on + strand, within GFF113 at 104.880 kb on + strand, within GFF113 at 104.883 kb on - strand, within GFF113 at 104.888 kb on - strand, within GFF113 at 104.888 kb on - strand, within GFF113 at 104.888 kb on - strand, within GFF113 at 104.910 kb on + strand, within GFF113 at 104.910 kb on + strand, within GFF113 at 104.918 kb on - strand, within GFF113 at 104.941 kb on - strand, within GFF113 at 104.980 kb on + strand, within GFF113 at 104.980 kb on + strand, within GFF113 at 104.980 kb on + strand, within GFF113 at 104.988 kb on - strand, within GFF113 at 104.988 kb on - strand, within GFF113 at 105.002 kb on + strand, within GFF113 at 105.010 kb on - strand, within GFF113 at 105.048 kb on + strand at 105.049 kb on - strand at 105.051 kb on - strand at 105.053 kb on + strand at 105.053 kb on + strand at 105.061 kb on - strand at 105.061 kb on - strand at 105.081 kb on + strand at 105.081 kb on + strand at 105.081 kb on + strand at 105.081 kb on + strand at 105.081 kb on + strand at 105.081 kb on - strand at 105.131 kb on - strand at 105.136 kb on + strand at 105.139 kb on - strand at 105.139 kb on - strand at 105.184 kb on - strand at 105.184 kb on - strand at 105.221 kb on + strand at 105.259 kb on + strand, within GFF114 at 105.260 kb on + strand, within GFF114 at 105.260 kb on + strand, within GFF114 at 105.260 kb on + strand, within GFF114 at 105.260 kb on + strand, within GFF114 at 105.260 kb on + strand, within GFF114 at 105.276 kb on + strand, within GFF114 at 105.286 kb on + strand, within GFF114 at 105.287 kb on - strand, within GFF114 at 105.292 kb on - strand, within GFF114 at 105.329 kb on + strand, within GFF114 at 105.329 kb on + strand, within GFF114 at 105.334 kb on + strand, within GFF114 at 105.337 kb on - strand, within GFF114 at 105.337 kb on - strand, within GFF114 at 105.350 kb on - strand, within GFF114 at 105.350 kb on - strand, within GFF114 at 105.367 kb on - strand, within GFF114 at 105.367 kb on - strand, within GFF114 at 105.400 kb on + strand, within GFF114 at 105.425 kb on + strand, within GFF114 at 105.440 kb on + strand, within GFF114 at 105.453 kb on + strand, within GFF114 at 105.476 kb on + strand, within GFF114 at 105.491 kb on + strand, within GFF114 at 105.491 kb on + strand, within GFF114 at 105.512 kb on + strand, within GFF114 at 105.512 kb on + strand, within GFF114 at 105.512 kb on + strand, within GFF114 at 105.512 kb on + strand, within GFF114 at 105.512 kb on + strand, within GFF114 at 105.520 kb on - strand, within GFF114 at 105.520 kb on - strand, within GFF114 at 105.520 kb on - strand, within GFF114 at 105.526 kb on - strand, within GFF114 at 105.555 kb on - strand, within GFF114 at 105.555 kb on - strand, within GFF114 at 105.555 kb on - strand, within GFF114 at 105.601 kb on - strand, within GFF114 at 105.603 kb on + strand, within GFF114 at 105.603 kb on + strand, within GFF114 at 105.611 kb on - strand, within GFF114 at 105.631 kb on + strand, within GFF114 at 105.639 kb on + strand, within GFF114 at 105.639 kb on - strand, within GFF114 at 105.656 kb on + strand, within GFF114 at 105.669 kb on + strand, within GFF114 at 105.669 kb on + strand, within GFF114 at 105.669 kb on + strand, within GFF114 at 105.677 kb on - strand, within GFF114 at 105.677 kb on - strand, within GFF114 at 105.681 kb on + strand, within GFF114 at 105.682 kb on + strand, within GFF114 at 105.689 kb on + strand, within GFF114 at 105.689 kb on + strand, within GFF114 at 105.689 kb on + strand, within GFF114 at 105.697 kb on - strand, within GFF114 at 105.697 kb on - strand, within GFF114 at 105.699 kb on - strand, within GFF114 at 105.710 kb on - strand, within GFF114 at 105.715 kb on - strand, within GFF114 at 105.722 kb on + strand, within GFF114 at 105.735 kb on - strand, within GFF114 at 105.737 kb on - strand, within GFF114 at 105.739 kb on - strand, within GFF114 at 105.739 kb on - strand, within GFF114 at 105.749 kb on + strand, within GFF114 at 105.749 kb on + strand, within GFF114 at 105.770 kb on + strand, within GFF114 at 105.778 kb on - strand, within GFF114 at 105.788 kb on - strand, within GFF114 at 105.793 kb on - strand, within GFF114 at 105.798 kb on - strand, within GFF114 at 105.825 kb on + strand, within GFF114 at 105.835 kb on + strand, within GFF114 at 105.865 kb on - strand, within GFF114 at 105.914 kb on - strand, within GFF114 at 105.916 kb on + strand, within GFF114 at 105.919 kb on - strand, within GFF114 at 105.932 kb on - strand, within GFF114 at 105.973 kb on + strand at 105.981 kb on - strand at 105.982 kb on - strand at 105.982 kb on - strand at 106.031 kb on - strand at 106.101 kb on + strand at 106.118 kb on + strand at 106.128 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Ammonium Sulfate remove 104,163 + GFF113 0.11 -1.3 104,236 - GFF113 0.18 +0.5 104,280 + GFF113 0.22 +0.2 104,283 - GFF113 0.23 +0.4 104,288 - GFF113 0.23 -0.3 104,288 - GFF113 0.23 +0.8 104,288 - GFF113 0.23 +0.0 104,288 - GFF113 0.23 -1.3 104,288 - GFF113 0.23 -0.7 104,307 - GFF113 0.25 -0.1 104,307 - GFF113 0.25 -0.9 104,323 + GFF113 0.26 +1.9 104,342 - GFF113 0.28 -0.6 104,356 + GFF113 0.29 +0.0 104,356 + GFF113 0.29 -1.0 104,356 + GFF113 0.29 -0.0 104,361 + GFF113 0.30 +1.3 104,416 + GFF113 0.35 -0.3 104,435 + GFF113 0.37 +0.4 104,453 + GFF113 0.38 -1.2 104,455 - GFF113 0.38 +0.3 104,461 - GFF113 0.39 +1.4 104,465 - GFF113 0.39 -1.4 104,478 - GFF113 0.41 -0.9 104,531 - GFF113 0.45 -1.0 104,583 + GFF113 0.50 +0.7 104,583 + GFF113 0.50 +0.9 104,583 + GFF113 0.50 +0.1 104,591 - GFF113 0.51 -0.5 104,591 - GFF113 0.51 -0.8 104,598 + GFF113 0.52 -0.7 104,598 + GFF113 0.52 -0.3 104,602 + GFF113 0.52 -0.2 104,606 - GFF113 0.52 +0.6 104,625 + GFF113 0.54 +1.6 104,633 - GFF113 0.55 -0.2 104,633 - GFF113 0.55 -1.4 104,652 - GFF113 0.57 +0.3 104,673 - GFF113 0.59 +1.8 104,676 + GFF113 0.59 +0.9 104,686 + GFF113 0.60 -0.0 104,686 + GFF113 0.60 -0.3 104,688 + GFF113 0.60 +2.4 104,693 + GFF113 0.60 +0.5 104,694 - GFF113 0.60 +0.4 104,694 - GFF113 0.60 -0.7 104,701 - GFF113 0.61 -1.9 104,733 + GFF113 0.64 -0.5 104,733 + GFF113 0.64 -0.9 104,733 + GFF113 0.64 -0.2 104,736 - GFF113 0.64 +0.8 104,740 + GFF113 0.65 -0.1 104,741 - GFF113 0.65 -0.9 104,741 - GFF113 0.65 -0.8 104,741 - GFF113 0.65 -0.8 104,748 - GFF113 0.65 -1.0 104,764 + GFF113 0.67 +0.6 104,782 - GFF113 0.69 +0.4 104,806 + GFF113 0.71 +1.4 104,806 + GFF113 0.71 -0.7 104,814 - GFF113 0.72 +1.1 104,816 + GFF113 0.72 -0.7 104,824 - GFF113 0.72 -0.7 104,848 - GFF113 0.75 +1.6 104,851 - GFF113 0.75 -1.1 104,852 + GFF113 0.75 +0.4 104,880 + GFF113 0.78 +0.4 104,880 + GFF113 0.78 +1.0 104,880 + GFF113 0.78 +0.1 104,883 - GFF113 0.78 -0.3 104,888 - GFF113 0.78 +0.4 104,888 - GFF113 0.78 -0.6 104,888 - GFF113 0.78 +0.1 104,910 + GFF113 0.80 +0.1 104,910 + GFF113 0.80 -0.6 104,918 - GFF113 0.81 -0.8 104,941 - GFF113 0.83 -0.5 104,980 + GFF113 0.87 +1.2 104,980 + GFF113 0.87 -1.3 104,980 + GFF113 0.87 -0.0 104,988 - GFF113 0.88 -0.5 104,988 - GFF113 0.88 +3.6 105,002 + GFF113 0.89 +0.3 105,010 - GFF113 0.90 +0.1 105,048 + -0.3 105,049 - -0.6 105,051 - +0.8 105,053 + +0.0 105,053 + -0.8 105,061 - +1.6 105,061 - +1.3 105,081 + +0.6 105,081 + -0.9 105,081 + +0.1 105,081 + +0.5 105,081 + +0.9 105,081 - +1.0 105,131 - +0.4 105,136 + +1.3 105,139 - -2.7 105,139 - +0.7 105,184 - -0.9 105,184 - +0.1 105,221 + +1.0 105,259 + GFF114 0.13 -2.3 105,260 + GFF114 0.13 -1.6 105,260 + GFF114 0.13 +0.2 105,260 + GFF114 0.13 +0.1 105,260 + GFF114 0.13 +0.3 105,260 + GFF114 0.13 -2.8 105,276 + GFF114 0.15 +1.4 105,286 + GFF114 0.16 +0.1 105,287 - GFF114 0.16 -0.4 105,292 - GFF114 0.17 +0.5 105,329 + GFF114 0.21 -0.4 105,329 + GFF114 0.21 -0.9 105,334 + GFF114 0.22 +0.8 105,337 - GFF114 0.22 +0.1 105,337 - GFF114 0.22 -0.9 105,350 - GFF114 0.24 -0.6 105,350 - GFF114 0.24 -0.0 105,367 - GFF114 0.25 -0.3 105,367 - GFF114 0.25 -1.1 105,400 + GFF114 0.29 +0.7 105,425 + GFF114 0.32 +0.4 105,440 + GFF114 0.34 +0.1 105,453 + GFF114 0.35 -1.3 105,476 + GFF114 0.38 +1.4 105,491 + GFF114 0.40 +1.0 105,491 + GFF114 0.40 +0.0 105,512 + GFF114 0.42 +0.0 105,512 + GFF114 0.42 -0.7 105,512 + GFF114 0.42 +1.1 105,512 + GFF114 0.42 -0.6 105,512 + GFF114 0.42 -0.7 105,520 - GFF114 0.43 +0.7 105,520 - GFF114 0.43 -2.0 105,520 - GFF114 0.43 -0.0 105,526 - GFF114 0.44 -0.1 105,555 - GFF114 0.47 -1.4 105,555 - GFF114 0.47 +0.5 105,555 - GFF114 0.47 +0.7 105,601 - GFF114 0.52 -0.7 105,603 + GFF114 0.52 -0.0 105,603 + GFF114 0.52 -0.2 105,611 - GFF114 0.53 -1.3 105,631 + GFF114 0.56 +0.8 105,639 + GFF114 0.56 +0.5 105,639 - GFF114 0.56 +1.3 105,656 + GFF114 0.58 -1.1 105,669 + GFF114 0.60 +0.5 105,669 + GFF114 0.60 +0.5 105,669 + GFF114 0.60 -0.1 105,677 - GFF114 0.61 +0.7 105,677 - GFF114 0.61 +0.7 105,681 + GFF114 0.61 +1.9 105,682 + GFF114 0.61 +1.7 105,689 + GFF114 0.62 +0.5 105,689 + GFF114 0.62 -0.0 105,689 + GFF114 0.62 -1.6 105,697 - GFF114 0.63 -0.2 105,697 - GFF114 0.63 +1.3 105,699 - GFF114 0.63 +0.4 105,710 - GFF114 0.65 +0.4 105,715 - GFF114 0.65 +0.5 105,722 + GFF114 0.66 +1.1 105,735 - GFF114 0.67 +0.2 105,737 - GFF114 0.68 -1.9 105,739 - GFF114 0.68 -0.7 105,739 - GFF114 0.68 -0.3 105,749 + GFF114 0.69 +0.4 105,749 + GFF114 0.69 +0.8 105,770 + GFF114 0.71 -0.3 105,778 - GFF114 0.72 +0.3 105,788 - GFF114 0.73 -0.5 105,793 - GFF114 0.74 -0.0 105,798 - GFF114 0.75 +0.1 105,825 + GFF114 0.78 -0.6 105,835 + GFF114 0.79 +0.4 105,865 - GFF114 0.82 -0.3 105,914 - GFF114 0.88 +0.0 105,916 + GFF114 0.88 -1.2 105,919 - GFF114 0.88 -0.0 105,932 - GFF114 0.90 -0.2 105,973 + +0.4 105,981 - +0.3 105,982 - -0.1 105,982 - -0.0 106,031 - -0.2 106,101 + -1.6 106,118 + -0.4 106,128 - -0.2
Or see this region's nucleotide sequence