Experiment: EMM_noCarbon with Sucrose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF45 and GFF46 are separated by 45 nucleotides GFF46 and GFF47 are separated by 74 nucleotides GFF47 and GFF48 are separated by 4 nucleotides
GFF45 - secreted alkaline phosphatase, at 34,694 to 36,244
GFF45
GFF46 - Alkaline phosphodiesterase I (EC 3.1.4.1) / Nucleotide pyrophosphatase (EC 3.6.1.9), at 36,290 to 37,570
GFF46
GFF47 - G:T/U mismatch-specific uracil/thymine DNA-glycosylase (EC 3.2.2.27), at 37,645 to 38,172
GFF47
GFF48 - GTP-binding and nucleic acid-binding protein YchF, at 38,177 to 39,277
GFF48
Position (kb) 36
37
38 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 35.291 kb on - strand, within GFF45 at 35.296 kb on - strand, within GFF45 at 35.296 kb on - strand, within GFF45 at 35.359 kb on - strand, within GFF45 at 35.375 kb on - strand, within GFF45 at 35.392 kb on - strand, within GFF45 at 35.421 kb on + strand, within GFF45 at 35.566 kb on + strand, within GFF45 at 35.566 kb on + strand, within GFF45 at 35.566 kb on + strand, within GFF45 at 35.581 kb on + strand, within GFF45 at 35.607 kb on - strand, within GFF45 at 35.614 kb on + strand, within GFF45 at 35.619 kb on + strand, within GFF45 at 35.619 kb on + strand, within GFF45 at 35.622 kb on - strand, within GFF45 at 35.647 kb on + strand, within GFF45 at 35.653 kb on - strand, within GFF45 at 35.653 kb on - strand, within GFF45 at 35.655 kb on - strand, within GFF45 at 35.700 kb on - strand, within GFF45 at 35.700 kb on - strand, within GFF45 at 35.732 kb on + strand, within GFF45 at 35.740 kb on - strand, within GFF45 at 35.766 kb on + strand, within GFF45 at 35.767 kb on - strand, within GFF45 at 35.774 kb on - strand, within GFF45 at 35.774 kb on - strand, within GFF45 at 35.821 kb on + strand, within GFF45 at 35.821 kb on + strand, within GFF45 at 35.821 kb on + strand, within GFF45 at 35.821 kb on + strand, within GFF45 at 35.821 kb on + strand, within GFF45 at 35.821 kb on + strand, within GFF45 at 35.821 kb on + strand, within GFF45 at 35.821 kb on + strand, within GFF45 at 35.821 kb on + strand, within GFF45 at 35.829 kb on - strand, within GFF45 at 35.829 kb on - strand, within GFF45 at 35.829 kb on - strand, within GFF45 at 35.829 kb on - strand, within GFF45 at 35.829 kb on - strand, within GFF45 at 35.829 kb on - strand, within GFF45 at 35.829 kb on - strand, within GFF45 at 35.848 kb on + strand, within GFF45 at 35.850 kb on + strand, within GFF45 at 35.850 kb on + strand, within GFF45 at 35.858 kb on - strand, within GFF45 at 35.863 kb on - strand, within GFF45 at 35.865 kb on + strand, within GFF45 at 35.865 kb on + strand, within GFF45 at 35.865 kb on + strand, within GFF45 at 35.865 kb on + strand, within GFF45 at 35.875 kb on + strand, within GFF45 at 35.875 kb on + strand, within GFF45 at 35.883 kb on - strand, within GFF45 at 35.883 kb on - strand, within GFF45 at 35.887 kb on + strand, within GFF45 at 35.901 kb on + strand, within GFF45 at 35.901 kb on - strand, within GFF45 at 35.909 kb on - strand, within GFF45 at 35.994 kb on + strand, within GFF45 at 36.035 kb on + strand, within GFF45 at 36.085 kb on + strand, within GFF45 at 36.102 kb on + strand at 36.129 kb on + strand at 36.129 kb on + strand at 36.129 kb on + strand at 36.129 kb on + strand at 36.129 kb on + strand at 36.129 kb on + strand at 36.129 kb on + strand at 36.137 kb on - strand at 36.137 kb on - strand at 36.137 kb on - strand at 36.137 kb on - strand at 36.146 kb on + strand at 36.154 kb on - strand at 36.224 kb on - strand at 36.253 kb on + strand at 36.261 kb on - strand at 36.288 kb on + strand at 36.294 kb on + strand at 36.294 kb on + strand at 36.308 kb on + strand at 36.308 kb on + strand at 36.316 kb on - strand at 36.324 kb on - strand at 36.356 kb on - strand at 36.419 kb on + strand, within GFF46 at 36.427 kb on - strand, within GFF46 at 36.449 kb on + strand, within GFF46 at 36.478 kb on + strand, within GFF46 at 36.487 kb on - strand, within GFF46 at 36.495 kb on + strand, within GFF46 at 36.547 kb on + strand, within GFF46 at 36.574 kb on + strand, within GFF46 at 36.603 kb on - strand, within GFF46 at 36.622 kb on + strand, within GFF46 at 36.622 kb on + strand, within GFF46 at 36.622 kb on + strand, within GFF46 at 36.622 kb on + strand, within GFF46 at 36.625 kb on - strand, within GFF46 at 36.630 kb on - strand, within GFF46 at 36.630 kb on - strand, within GFF46 at 36.636 kb on + strand, within GFF46 at 36.636 kb on + strand, within GFF46 at 36.644 kb on - strand, within GFF46 at 36.644 kb on - strand, within GFF46 at 36.688 kb on + strand, within GFF46 at 36.688 kb on + strand, within GFF46 at 36.692 kb on + strand, within GFF46 at 36.694 kb on + strand, within GFF46 at 36.699 kb on + strand, within GFF46 at 36.707 kb on - strand, within GFF46 at 36.712 kb on - strand, within GFF46 at 36.777 kb on + strand, within GFF46 at 36.795 kb on - strand, within GFF46 at 36.795 kb on - strand, within GFF46 at 36.802 kb on + strand, within GFF46 at 36.802 kb on - strand, within GFF46 at 36.921 kb on + strand, within GFF46 at 36.943 kb on + strand, within GFF46 at 36.962 kb on + strand, within GFF46 at 36.985 kb on - strand, within GFF46 at 36.985 kb on - strand, within GFF46 at 37.009 kb on + strand, within GFF46 at 37.009 kb on + strand, within GFF46 at 37.009 kb on + strand, within GFF46 at 37.017 kb on - strand, within GFF46 at 37.017 kb on - strand, within GFF46 at 37.017 kb on - strand, within GFF46 at 37.017 kb on - strand, within GFF46 at 37.017 kb on - strand, within GFF46 at 37.017 kb on - strand, within GFF46 at 37.034 kb on - strand, within GFF46 at 37.041 kb on - strand, within GFF46 at 37.044 kb on - strand, within GFF46 at 37.077 kb on - strand, within GFF46 at 37.080 kb on - strand, within GFF46 at 37.130 kb on - strand, within GFF46 at 37.132 kb on - strand, within GFF46 at 37.135 kb on - strand, within GFF46 at 37.142 kb on + strand, within GFF46 at 37.166 kb on + strand, within GFF46 at 37.177 kb on + strand, within GFF46 at 37.182 kb on + strand, within GFF46 at 37.186 kb on + strand, within GFF46 at 37.194 kb on - strand, within GFF46 at 37.204 kb on - strand, within GFF46 at 37.204 kb on - strand, within GFF46 at 37.284 kb on - strand, within GFF46 at 37.320 kb on + strand, within GFF46 at 37.334 kb on + strand, within GFF46 at 37.338 kb on - strand, within GFF46 at 37.351 kb on - strand, within GFF46 at 37.356 kb on - strand, within GFF46 at 37.375 kb on + strand, within GFF46 at 37.381 kb on + strand, within GFF46 at 37.382 kb on - strand, within GFF46 at 37.389 kb on - strand, within GFF46 at 37.535 kb on - strand at 37.537 kb on + strand at 37.545 kb on - strand at 37.545 kb on - strand at 37.545 kb on - strand at 37.545 kb on - strand at 37.545 kb on - strand at 37.545 kb on - strand at 37.566 kb on - strand at 37.568 kb on + strand at 37.604 kb on + strand at 37.604 kb on + strand at 37.604 kb on + strand at 37.604 kb on + strand at 37.604 kb on + strand at 37.612 kb on - strand at 37.612 kb on - strand at 37.612 kb on - strand at 37.612 kb on - strand at 37.614 kb on - strand at 37.622 kb on - strand at 37.622 kb on - strand at 37.635 kb on + strand at 37.651 kb on - strand at 37.655 kb on - strand at 37.661 kb on - strand at 37.665 kb on + strand at 37.676 kb on + strand at 37.676 kb on + strand at 37.676 kb on + strand at 37.676 kb on + strand at 37.676 kb on + strand at 37.684 kb on - strand at 37.684 kb on - strand at 37.684 kb on - strand at 37.684 kb on - strand at 37.684 kb on - strand at 37.727 kb on + strand, within GFF47 at 37.735 kb on - strand, within GFF47 at 37.735 kb on - strand, within GFF47 at 37.737 kb on + strand, within GFF47 at 37.750 kb on - strand, within GFF47 at 37.780 kb on + strand, within GFF47 at 37.837 kb on + strand, within GFF47 at 37.845 kb on - strand, within GFF47 at 37.858 kb on + strand, within GFF47 at 37.863 kb on + strand, within GFF47 at 37.863 kb on + strand, within GFF47 at 37.863 kb on + strand, within GFF47 at 37.863 kb on + strand, within GFF47 at 37.871 kb on - strand, within GFF47 at 37.871 kb on - strand, within GFF47 at 37.871 kb on - strand, within GFF47 at 37.871 kb on - strand, within GFF47 at 37.885 kb on + strand, within GFF47 at 37.888 kb on - strand, within GFF47 at 37.888 kb on - strand, within GFF47 at 37.890 kb on - strand, within GFF47 at 37.920 kb on + strand, within GFF47 at 37.924 kb on + strand, within GFF47 at 37.924 kb on + strand, within GFF47 at 37.929 kb on + strand, within GFF47 at 37.929 kb on + strand, within GFF47 at 37.939 kb on - strand, within GFF47 at 37.943 kb on + strand, within GFF47 at 37.947 kb on - strand, within GFF47 at 37.952 kb on + strand, within GFF47 at 37.971 kb on + strand, within GFF47 at 37.974 kb on - strand, within GFF47 at 37.981 kb on + strand, within GFF47 at 37.981 kb on + strand, within GFF47 at 37.984 kb on - strand, within GFF47 at 37.989 kb on - strand, within GFF47 at 38.011 kb on - strand, within GFF47 at 38.021 kb on - strand, within GFF47 at 38.024 kb on + strand, within GFF47 at 38.045 kb on - strand, within GFF47 at 38.063 kb on - strand, within GFF47 at 38.063 kb on - strand, within GFF47 at 38.063 kb on - strand, within GFF47 at 38.117 kb on - strand, within GFF47 at 38.117 kb on - strand, within GFF47 at 38.117 kb on - strand, within GFF47 at 38.142 kb on + strand at 38.142 kb on + strand at 38.175 kb on + strand at 38.295 kb on + strand, within GFF48 at 38.478 kb on + strand, within GFF48
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Ammonium Sulfate remove 35,291 - GFF45 0.38 +0.2 35,296 - GFF45 0.39 +0.4 35,296 - GFF45 0.39 +1.3 35,359 - GFF45 0.43 +0.7 35,375 - GFF45 0.44 -0.0 35,392 - GFF45 0.45 -2.6 35,421 + GFF45 0.47 +0.5 35,566 + GFF45 0.56 +0.4 35,566 + GFF45 0.56 -0.2 35,566 + GFF45 0.56 +0.2 35,581 + GFF45 0.57 +0.9 35,607 - GFF45 0.59 +0.4 35,614 + GFF45 0.59 -0.4 35,619 + GFF45 0.60 +0.1 35,619 + GFF45 0.60 -0.2 35,622 - GFF45 0.60 -0.8 35,647 + GFF45 0.61 -0.9 35,653 - GFF45 0.62 +0.4 35,653 - GFF45 0.62 +0.3 35,655 - GFF45 0.62 +0.2 35,700 - GFF45 0.65 +0.9 35,700 - GFF45 0.65 -1.0 35,732 + GFF45 0.67 -1.4 35,740 - GFF45 0.67 -0.1 35,766 + GFF45 0.69 +0.8 35,767 - GFF45 0.69 -1.7 35,774 - GFF45 0.70 -0.7 35,774 - GFF45 0.70 -0.3 35,821 + GFF45 0.73 +0.3 35,821 + GFF45 0.73 +0.4 35,821 + GFF45 0.73 +0.2 35,821 + GFF45 0.73 -0.4 35,821 + GFF45 0.73 -1.0 35,821 + GFF45 0.73 +1.8 35,821 + GFF45 0.73 -0.6 35,821 + GFF45 0.73 +0.1 35,821 + GFF45 0.73 -0.8 35,829 - GFF45 0.73 -1.3 35,829 - GFF45 0.73 -0.4 35,829 - GFF45 0.73 -1.3 35,829 - GFF45 0.73 -0.4 35,829 - GFF45 0.73 +0.8 35,829 - GFF45 0.73 +0.1 35,829 - GFF45 0.73 -0.8 35,848 + GFF45 0.74 +1.5 35,850 + GFF45 0.75 -1.3 35,850 + GFF45 0.75 -0.8 35,858 - GFF45 0.75 +0.6 35,863 - GFF45 0.75 +0.6 35,865 + GFF45 0.75 +0.3 35,865 + GFF45 0.75 +1.4 35,865 + GFF45 0.75 +1.9 35,865 + GFF45 0.75 -1.5 35,875 + GFF45 0.76 -1.0 35,875 + GFF45 0.76 +1.0 35,883 - GFF45 0.77 -0.1 35,883 - GFF45 0.77 +1.6 35,887 + GFF45 0.77 -0.3 35,901 + GFF45 0.78 -0.8 35,901 - GFF45 0.78 +2.4 35,909 - GFF45 0.78 -0.8 35,994 + GFF45 0.84 +1.1 36,035 + GFF45 0.86 +2.0 36,085 + GFF45 0.90 +0.6 36,102 + -0.2 36,129 + +0.1 36,129 + -0.3 36,129 + +0.3 36,129 + -1.2 36,129 + +2.1 36,129 + +0.9 36,129 + -0.4 36,137 - -0.2 36,137 - +0.4 36,137 - +0.4 36,137 - +1.4 36,146 + +0.5 36,154 - -0.2 36,224 - -1.3 36,253 + +0.8 36,261 - +0.2 36,288 + -1.5 36,294 + +1.8 36,294 + +0.1 36,308 + +0.4 36,308 + +0.8 36,316 - -0.7 36,324 - -0.4 36,356 - -0.7 36,419 + GFF46 0.10 +0.0 36,427 - GFF46 0.11 +0.1 36,449 + GFF46 0.12 -0.3 36,478 + GFF46 0.15 +0.1 36,487 - GFF46 0.15 -3.1 36,495 + GFF46 0.16 -0.0 36,547 + GFF46 0.20 -0.5 36,574 + GFF46 0.22 -0.2 36,603 - GFF46 0.24 +0.6 36,622 + GFF46 0.26 -0.9 36,622 + GFF46 0.26 +1.3 36,622 + GFF46 0.26 +0.5 36,622 + GFF46 0.26 +0.2 36,625 - GFF46 0.26 -0.0 36,630 - GFF46 0.27 -0.3 36,630 - GFF46 0.27 -1.0 36,636 + GFF46 0.27 -1.4 36,636 + GFF46 0.27 +0.6 36,644 - GFF46 0.28 -0.7 36,644 - GFF46 0.28 -1.1 36,688 + GFF46 0.31 -1.3 36,688 + GFF46 0.31 +0.4 36,692 + GFF46 0.31 -1.9 36,694 + GFF46 0.32 +0.2 36,699 + GFF46 0.32 +0.4 36,707 - GFF46 0.33 +0.5 36,712 - GFF46 0.33 +0.9 36,777 + GFF46 0.38 -1.1 36,795 - GFF46 0.39 -1.0 36,795 - GFF46 0.39 -0.7 36,802 + GFF46 0.40 -0.1 36,802 - GFF46 0.40 -2.3 36,921 + GFF46 0.49 +0.2 36,943 + GFF46 0.51 +0.5 36,962 + GFF46 0.52 -2.6 36,985 - GFF46 0.54 -0.2 36,985 - GFF46 0.54 +0.1 37,009 + GFF46 0.56 -2.1 37,009 + GFF46 0.56 -0.3 37,009 + GFF46 0.56 -1.1 37,017 - GFF46 0.57 +0.7 37,017 - GFF46 0.57 +0.6 37,017 - GFF46 0.57 -0.8 37,017 - GFF46 0.57 -0.4 37,017 - GFF46 0.57 -0.1 37,017 - GFF46 0.57 +1.1 37,034 - GFF46 0.58 -0.7 37,041 - GFF46 0.59 -2.2 37,044 - GFF46 0.59 -0.3 37,077 - GFF46 0.61 +0.6 37,080 - GFF46 0.62 -0.9 37,130 - GFF46 0.66 -0.4 37,132 - GFF46 0.66 -0.4 37,135 - GFF46 0.66 -1.0 37,142 + GFF46 0.67 +1.4 37,166 + GFF46 0.68 +0.8 37,177 + GFF46 0.69 +0.1 37,182 + GFF46 0.70 -2.1 37,186 + GFF46 0.70 +0.4 37,194 - GFF46 0.71 +0.1 37,204 - GFF46 0.71 +1.5 37,204 - GFF46 0.71 +2.0 37,284 - GFF46 0.78 -0.3 37,320 + GFF46 0.80 -1.3 37,334 + GFF46 0.81 -1.3 37,338 - GFF46 0.82 -0.6 37,351 - GFF46 0.83 +0.1 37,356 - GFF46 0.83 -1.9 37,375 + GFF46 0.85 -0.3 37,381 + GFF46 0.85 -0.6 37,382 - GFF46 0.85 -0.2 37,389 - GFF46 0.86 -0.1 37,535 - +0.3 37,537 + +1.0 37,545 - -1.0 37,545 - -1.2 37,545 - -0.4 37,545 - -0.9 37,545 - -0.8 37,545 - -0.5 37,566 - +0.8 37,568 + +0.2 37,604 + -0.6 37,604 + -1.0 37,604 + +0.8 37,604 + -1.0 37,604 + +0.0 37,612 - -0.7 37,612 - +1.8 37,612 - -0.6 37,612 - +0.4 37,614 - +1.2 37,622 - -1.2 37,622 - -1.1 37,635 + -1.0 37,651 - +0.2 37,655 - +0.6 37,661 - -1.7 37,665 + +0.1 37,676 + -0.6 37,676 + -0.7 37,676 + -0.0 37,676 + +1.1 37,676 + +0.2 37,684 - -0.2 37,684 - -0.7 37,684 - -1.0 37,684 - -0.8 37,684 - -0.0 37,727 + GFF47 0.16 +0.0 37,735 - GFF47 0.17 +0.4 37,735 - GFF47 0.17 -0.2 37,737 + GFF47 0.17 +0.5 37,750 - GFF47 0.20 +0.5 37,780 + GFF47 0.26 -0.8 37,837 + GFF47 0.36 -0.1 37,845 - GFF47 0.38 -0.8 37,858 + GFF47 0.40 +0.9 37,863 + GFF47 0.41 +0.6 37,863 + GFF47 0.41 -0.3 37,863 + GFF47 0.41 +0.4 37,863 + GFF47 0.41 +0.4 37,871 - GFF47 0.43 +0.4 37,871 - GFF47 0.43 -0.4 37,871 - GFF47 0.43 +0.9 37,871 - GFF47 0.43 -0.4 37,885 + GFF47 0.45 +2.3 37,888 - GFF47 0.46 -0.3 37,888 - GFF47 0.46 -0.3 37,890 - GFF47 0.46 -0.2 37,920 + GFF47 0.52 -0.5 37,924 + GFF47 0.53 +0.6 37,924 + GFF47 0.53 -0.9 37,929 + GFF47 0.54 +1.4 37,929 + GFF47 0.54 -1.0 37,939 - GFF47 0.56 -0.1 37,943 + GFF47 0.56 -1.1 37,947 - GFF47 0.57 -1.1 37,952 + GFF47 0.58 +0.4 37,971 + GFF47 0.62 +0.6 37,974 - GFF47 0.62 -0.9 37,981 + GFF47 0.64 +2.1 37,981 + GFF47 0.64 +0.1 37,984 - GFF47 0.64 +0.4 37,989 - GFF47 0.65 -0.0 38,011 - GFF47 0.69 -0.6 38,021 - GFF47 0.71 +0.2 38,024 + GFF47 0.72 -1.2 38,045 - GFF47 0.76 -1.7 38,063 - GFF47 0.79 -0.8 38,063 - GFF47 0.79 +0.1 38,063 - GFF47 0.79 +0.7 38,117 - GFF47 0.89 +0.2 38,117 - GFF47 0.89 +2.4 38,117 - GFF47 0.89 +0.9 38,142 + -0.9 38,142 + +0.2 38,175 + +0.1 38,295 + GFF48 0.11 +1.1 38,478 + GFF48 0.27 +0.8
Or see this region's nucleotide sequence