Experiment: EMM_noCarbon with Sucrose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3239 and GFF3240 are separated by 155 nucleotides GFF3240 and GFF3241 are separated by 84 nucleotides GFF3241 and GFF3242 overlap by 11 nucleotides GFF3242 and GFF3243 are separated by 66 nucleotides
GFF3239 - Thiamine-monophosphate kinase (EC 2.7.4.16), at 3,295,351 to 3,296,268
GFF3239
GFF3240 - Transcription termination protein NusB, at 3,296,424 to 3,296,870
GFF3240
GFF3241 - Histidinol dehydrogenase (EC 1.1.1.23), at 3,296,955 to 3,298,241
GFF3241
GFF3242 - ATP phosphoribosyltransferase (EC 2.4.2.17) => HisGs, at 3,298,231 to 3,298,893
GFF3242
GFF3243 - Inorganic triphosphatase (EC 3.6.1.25), at 3,298,960 to 3,300,423
GFF3243
Position (kb)
3296
3297
3298
3299 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3296.277 kb on + strand at 3296.277 kb on + strand at 3296.277 kb on - strand at 3296.283 kb on - strand at 3296.285 kb on - strand at 3296.289 kb on - strand at 3296.347 kb on + strand at 3296.361 kb on + strand at 3296.383 kb on + strand at 3296.398 kb on + strand at 3296.437 kb on + strand at 3296.445 kb on - strand at 3296.445 kb on - strand at 3296.449 kb on + strand at 3296.457 kb on - strand at 3296.899 kb on - strand at 3296.901 kb on + strand at 3296.904 kb on - strand at 3296.909 kb on - strand at 3296.930 kb on - strand at 3296.930 kb on - strand at 3296.930 kb on - strand at 3296.930 kb on - strand at 3296.932 kb on + strand at 3296.932 kb on + strand at 3296.932 kb on + strand at 3296.940 kb on - strand at 3297.001 kb on - strand at 3297.003 kb on - strand at 3297.012 kb on + strand at 3297.050 kb on + strand at 3297.058 kb on - strand at 3297.065 kb on - strand at 3297.067 kb on - strand at 3297.092 kb on - strand, within GFF3241 at 3297.098 kb on + strand, within GFF3241 at 3297.098 kb on + strand, within GFF3241 at 3297.127 kb on + strand, within GFF3241 at 3297.135 kb on + strand, within GFF3241 at 3297.141 kb on + strand, within GFF3241 at 3297.142 kb on - strand, within GFF3241 at 3297.149 kb on - strand, within GFF3241 at 3297.164 kb on - strand, within GFF3241 at 3297.205 kb on + strand, within GFF3241 at 3297.208 kb on + strand, within GFF3241 at 3297.208 kb on + strand, within GFF3241 at 3297.208 kb on + strand, within GFF3241 at 3297.208 kb on + strand, within GFF3241 at 3297.216 kb on - strand, within GFF3241 at 3297.223 kb on + strand, within GFF3241 at 3297.226 kb on - strand, within GFF3241 at 3297.239 kb on + strand, within GFF3241 at 3297.304 kb on - strand, within GFF3241 at 3297.347 kb on - strand, within GFF3241 at 3297.357 kb on - strand, within GFF3241 at 3297.362 kb on - strand, within GFF3241 at 3297.389 kb on - strand, within GFF3241 at 3297.395 kb on + strand, within GFF3241 at 3297.395 kb on + strand, within GFF3241 at 3297.395 kb on + strand, within GFF3241 at 3297.427 kb on + strand, within GFF3241 at 3297.466 kb on - strand, within GFF3241 at 3297.468 kb on + strand, within GFF3241 at 3297.476 kb on - strand, within GFF3241 at 3297.477 kb on - strand, within GFF3241 at 3297.487 kb on + strand, within GFF3241 at 3297.501 kb on + strand, within GFF3241 at 3297.509 kb on - strand, within GFF3241 at 3297.509 kb on - strand, within GFF3241 at 3297.509 kb on - strand, within GFF3241 at 3297.548 kb on + strand, within GFF3241 at 3297.552 kb on + strand, within GFF3241 at 3297.553 kb on - strand, within GFF3241 at 3297.556 kb on - strand, within GFF3241 at 3297.565 kb on + strand, within GFF3241 at 3297.565 kb on + strand, within GFF3241 at 3297.565 kb on + strand, within GFF3241 at 3297.565 kb on + strand, within GFF3241 at 3297.565 kb on + strand, within GFF3241 at 3297.565 kb on + strand, within GFF3241 at 3297.565 kb on + strand, within GFF3241 at 3297.570 kb on - strand, within GFF3241 at 3297.570 kb on - strand, within GFF3241 at 3297.572 kb on + strand, within GFF3241 at 3297.573 kb on - strand, within GFF3241 at 3297.573 kb on - strand, within GFF3241 at 3297.579 kb on - strand, within GFF3241 at 3297.600 kb on - strand, within GFF3241 at 3297.600 kb on - strand, within GFF3241 at 3297.628 kb on + strand, within GFF3241 at 3297.628 kb on + strand, within GFF3241 at 3297.636 kb on - strand, within GFF3241 at 3297.636 kb on - strand, within GFF3241 at 3297.641 kb on - strand, within GFF3241 at 3297.641 kb on - strand, within GFF3241 at 3297.641 kb on - strand, within GFF3241 at 3297.654 kb on - strand, within GFF3241 at 3297.654 kb on - strand, within GFF3241 at 3297.654 kb on - strand, within GFF3241 at 3297.659 kb on - strand, within GFF3241 at 3297.659 kb on - strand, within GFF3241 at 3297.661 kb on + strand, within GFF3241 at 3297.661 kb on + strand, within GFF3241 at 3297.669 kb on - strand, within GFF3241 at 3297.669 kb on - strand, within GFF3241 at 3297.711 kb on - strand, within GFF3241 at 3297.712 kb on - strand, within GFF3241 at 3297.720 kb on + strand, within GFF3241 at 3297.720 kb on + strand, within GFF3241 at 3297.746 kb on - strand, within GFF3241 at 3297.765 kb on - strand, within GFF3241 at 3297.768 kb on + strand, within GFF3241 at 3297.769 kb on + strand, within GFF3241 at 3297.777 kb on - strand, within GFF3241 at 3297.784 kb on + strand, within GFF3241 at 3297.784 kb on + strand, within GFF3241 at 3297.790 kb on + strand, within GFF3241 at 3297.798 kb on - strand, within GFF3241 at 3297.805 kb on + strand, within GFF3241 at 3297.805 kb on + strand, within GFF3241 at 3297.818 kb on - strand, within GFF3241 at 3297.833 kb on - strand, within GFF3241 at 3297.833 kb on - strand, within GFF3241 at 3297.833 kb on - strand, within GFF3241 at 3297.860 kb on - strand, within GFF3241 at 3297.872 kb on + strand, within GFF3241 at 3297.877 kb on + strand, within GFF3241 at 3297.893 kb on + strand, within GFF3241 at 3297.902 kb on - strand, within GFF3241 at 3297.907 kb on - strand, within GFF3241 at 3297.921 kb on + strand, within GFF3241 at 3297.924 kb on + strand, within GFF3241 at 3297.926 kb on + strand, within GFF3241 at 3297.926 kb on + strand, within GFF3241 at 3297.929 kb on - strand, within GFF3241 at 3297.932 kb on - strand, within GFF3241 at 3297.934 kb on - strand, within GFF3241 at 3297.952 kb on + strand, within GFF3241 at 3297.974 kb on + strand, within GFF3241 at 3297.987 kb on + strand, within GFF3241 at 3297.987 kb on + strand, within GFF3241 at 3297.987 kb on + strand, within GFF3241 at 3297.994 kb on + strand, within GFF3241 at 3297.994 kb on + strand, within GFF3241 at 3297.994 kb on + strand, within GFF3241 at 3297.995 kb on - strand, within GFF3241 at 3297.995 kb on - strand, within GFF3241 at 3297.995 kb on - strand, within GFF3241 at 3297.995 kb on - strand, within GFF3241 at 3298.002 kb on - strand, within GFF3241 at 3298.002 kb on - strand, within GFF3241 at 3298.007 kb on - strand, within GFF3241 at 3298.056 kb on + strand, within GFF3241 at 3298.078 kb on - strand, within GFF3241 at 3298.091 kb on - strand, within GFF3241 at 3298.097 kb on + strand, within GFF3241 at 3298.123 kb on + strand at 3298.123 kb on + strand at 3298.131 kb on - strand at 3298.133 kb on - strand at 3298.135 kb on + strand at 3298.135 kb on + strand at 3298.135 kb on + strand at 3298.143 kb on - strand at 3298.143 kb on - strand at 3298.143 kb on - strand at 3298.155 kb on + strand at 3298.190 kb on - strand at 3298.228 kb on + strand at 3298.248 kb on + strand at 3298.256 kb on + strand at 3298.256 kb on - strand at 3298.256 kb on - strand at 3298.257 kb on - strand at 3298.268 kb on + strand at 3298.268 kb on + strand at 3298.268 kb on + strand at 3298.268 kb on + strand at 3298.268 kb on + strand at 3298.268 kb on + strand at 3298.276 kb on - strand at 3298.276 kb on - strand at 3298.276 kb on - strand at 3298.276 kb on - strand at 3298.303 kb on + strand, within GFF3242 at 3298.303 kb on + strand, within GFF3242 at 3298.311 kb on - strand, within GFF3242 at 3298.311 kb on - strand, within GFF3242 at 3298.323 kb on + strand, within GFF3242 at 3298.343 kb on + strand, within GFF3242 at 3298.366 kb on - strand, within GFF3242 at 3298.376 kb on + strand, within GFF3242 at 3298.376 kb on + strand, within GFF3242 at 3298.399 kb on - strand, within GFF3242 at 3298.433 kb on - strand, within GFF3242 at 3298.544 kb on - strand, within GFF3242 at 3298.551 kb on - strand, within GFF3242 at 3298.551 kb on - strand, within GFF3242 at 3298.565 kb on + strand, within GFF3242 at 3298.618 kb on - strand, within GFF3242 at 3298.620 kb on - strand, within GFF3242 at 3298.620 kb on - strand, within GFF3242 at 3298.662 kb on + strand, within GFF3242 at 3298.667 kb on + strand, within GFF3242 at 3298.670 kb on - strand, within GFF3242 at 3298.670 kb on - strand, within GFF3242 at 3298.676 kb on + strand, within GFF3242 at 3298.676 kb on + strand, within GFF3242 at 3298.681 kb on + strand, within GFF3242 at 3298.681 kb on + strand, within GFF3242 at 3298.681 kb on + strand, within GFF3242 at 3298.684 kb on - strand, within GFF3242 at 3298.689 kb on - strand, within GFF3242 at 3298.689 kb on - strand, within GFF3242 at 3298.689 kb on - strand, within GFF3242 at 3298.689 kb on - strand, within GFF3242 at 3298.689 kb on - strand, within GFF3242 at 3298.689 kb on - strand, within GFF3242 at 3298.694 kb on - strand, within GFF3242 at 3298.694 kb on - strand, within GFF3242 at 3298.701 kb on - strand, within GFF3242 at 3298.716 kb on + strand, within GFF3242 at 3298.716 kb on + strand, within GFF3242 at 3298.716 kb on + strand, within GFF3242 at 3298.724 kb on - strand, within GFF3242 at 3298.773 kb on + strand, within GFF3242 at 3298.815 kb on - strand, within GFF3242 at 3298.829 kb on + strand at 3298.829 kb on + strand at 3298.842 kb on - strand at 3298.845 kb on + strand at 3298.853 kb on + strand at 3298.860 kb on - strand at 3298.897 kb on - strand at 3298.897 kb on - strand at 3298.911 kb on - strand at 3298.972 kb on - strand at 3298.979 kb on + strand at 3298.979 kb on + strand at 3298.981 kb on + strand at 3298.987 kb on - strand at 3298.987 kb on - strand at 3298.987 kb on - strand at 3298.989 kb on - strand at 3298.994 kb on + strand at 3299.022 kb on - strand at 3299.034 kb on - strand at 3299.055 kb on + strand at 3299.063 kb on - strand at 3299.063 kb on - strand at 3299.076 kb on + strand at 3299.094 kb on - strand at 3299.098 kb on + strand at 3299.128 kb on - strand, within GFF3243 at 3299.134 kb on - strand, within GFF3243 at 3299.135 kb on + strand, within GFF3243 at 3299.154 kb on + strand, within GFF3243 at 3299.167 kb on - strand, within GFF3243 at 3299.174 kb on + strand, within GFF3243 at 3299.183 kb on - strand, within GFF3243
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Ammonium Sulfate remove 3,296,277 + -1.2 3,296,277 + +0.2 3,296,277 - +0.2 3,296,283 - -1.1 3,296,285 - -0.8 3,296,289 - +0.7 3,296,347 + -2.1 3,296,361 + +1.0 3,296,383 + -1.0 3,296,398 + +0.6 3,296,437 + -0.7 3,296,445 - -1.1 3,296,445 - +0.4 3,296,449 + -0.5 3,296,457 - -1.0 3,296,899 - -0.5 3,296,901 + -1.0 3,296,904 - -1.2 3,296,909 - -1.0 3,296,930 - -1.5 3,296,930 - +0.9 3,296,930 - +0.1 3,296,930 - +0.5 3,296,932 + +0.8 3,296,932 + -1.1 3,296,932 + -0.7 3,296,940 - -0.6 3,297,001 - -0.6 3,297,003 - -1.3 3,297,012 + -1.4 3,297,050 + -1.4 3,297,058 - -2.2 3,297,065 - -0.7 3,297,067 - -0.5 3,297,092 - GFF3241 0.11 -1.2 3,297,098 + GFF3241 0.11 -1.7 3,297,098 + GFF3241 0.11 -0.7 3,297,127 + GFF3241 0.13 -2.0 3,297,135 + GFF3241 0.14 -0.9 3,297,141 + GFF3241 0.14 -1.2 3,297,142 - GFF3241 0.15 -1.2 3,297,149 - GFF3241 0.15 -0.7 3,297,164 - GFF3241 0.16 -1.0 3,297,205 + GFF3241 0.19 -0.9 3,297,208 + GFF3241 0.20 -1.9 3,297,208 + GFF3241 0.20 -0.8 3,297,208 + GFF3241 0.20 -1.1 3,297,208 + GFF3241 0.20 -1.4 3,297,216 - GFF3241 0.20 -1.0 3,297,223 + GFF3241 0.21 -1.2 3,297,226 - GFF3241 0.21 -1.9 3,297,239 + GFF3241 0.22 -1.2 3,297,304 - GFF3241 0.27 -0.6 3,297,347 - GFF3241 0.30 -2.1 3,297,357 - GFF3241 0.31 -3.1 3,297,362 - GFF3241 0.32 -1.2 3,297,389 - GFF3241 0.34 -2.6 3,297,395 + GFF3241 0.34 -1.5 3,297,395 + GFF3241 0.34 -0.6 3,297,395 + GFF3241 0.34 -1.2 3,297,427 + GFF3241 0.37 -1.5 3,297,466 - GFF3241 0.40 -0.7 3,297,468 + GFF3241 0.40 -0.9 3,297,476 - GFF3241 0.40 -1.2 3,297,477 - GFF3241 0.41 -1.7 3,297,487 + GFF3241 0.41 -0.7 3,297,501 + GFF3241 0.42 -0.7 3,297,509 - GFF3241 0.43 -1.1 3,297,509 - GFF3241 0.43 -2.2 3,297,509 - GFF3241 0.43 -1.2 3,297,548 + GFF3241 0.46 -1.0 3,297,552 + GFF3241 0.46 -2.0 3,297,553 - GFF3241 0.46 -1.3 3,297,556 - GFF3241 0.47 -0.7 3,297,565 + GFF3241 0.47 -1.2 3,297,565 + GFF3241 0.47 -1.1 3,297,565 + GFF3241 0.47 -0.5 3,297,565 + GFF3241 0.47 -1.5 3,297,565 + GFF3241 0.47 -0.5 3,297,565 + GFF3241 0.47 -2.1 3,297,565 + GFF3241 0.47 -1.4 3,297,570 - GFF3241 0.48 -1.7 3,297,570 - GFF3241 0.48 -0.5 3,297,572 + GFF3241 0.48 -0.5 3,297,573 - GFF3241 0.48 -0.8 3,297,573 - GFF3241 0.48 -1.6 3,297,579 - GFF3241 0.48 -0.7 3,297,600 - GFF3241 0.50 -1.9 3,297,600 - GFF3241 0.50 -2.2 3,297,628 + GFF3241 0.52 -1.2 3,297,628 + GFF3241 0.52 -2.4 3,297,636 - GFF3241 0.53 -1.0 3,297,636 - GFF3241 0.53 -2.5 3,297,641 - GFF3241 0.53 -1.1 3,297,641 - GFF3241 0.53 -0.8 3,297,641 - GFF3241 0.53 -0.6 3,297,654 - GFF3241 0.54 -2.1 3,297,654 - GFF3241 0.54 -1.2 3,297,654 - GFF3241 0.54 -2.1 3,297,659 - GFF3241 0.55 -0.5 3,297,659 - GFF3241 0.55 -1.4 3,297,661 + GFF3241 0.55 -2.3 3,297,661 + GFF3241 0.55 -1.8 3,297,669 - GFF3241 0.55 -1.4 3,297,669 - GFF3241 0.55 -1.0 3,297,711 - GFF3241 0.59 -2.8 3,297,712 - GFF3241 0.59 -0.5 3,297,720 + GFF3241 0.59 -0.8 3,297,720 + GFF3241 0.59 -0.7 3,297,746 - GFF3241 0.61 -1.1 3,297,765 - GFF3241 0.63 -1.2 3,297,768 + GFF3241 0.63 -0.9 3,297,769 + GFF3241 0.63 -0.8 3,297,777 - GFF3241 0.64 -1.9 3,297,784 + GFF3241 0.64 -1.3 3,297,784 + GFF3241 0.64 -1.8 3,297,790 + GFF3241 0.65 -0.7 3,297,798 - GFF3241 0.66 -2.5 3,297,805 + GFF3241 0.66 -0.9 3,297,805 + GFF3241 0.66 -1.0 3,297,818 - GFF3241 0.67 -0.6 3,297,833 - GFF3241 0.68 -1.2 3,297,833 - GFF3241 0.68 +0.5 3,297,833 - GFF3241 0.68 -0.6 3,297,860 - GFF3241 0.70 -1.1 3,297,872 + GFF3241 0.71 +0.5 3,297,877 + GFF3241 0.72 -2.3 3,297,893 + GFF3241 0.73 -0.8 3,297,902 - GFF3241 0.74 -0.8 3,297,907 - GFF3241 0.74 -1.1 3,297,921 + GFF3241 0.75 -0.9 3,297,924 + GFF3241 0.75 -1.5 3,297,926 + GFF3241 0.75 +0.2 3,297,926 + GFF3241 0.75 -0.5 3,297,929 - GFF3241 0.76 -0.6 3,297,932 - GFF3241 0.76 -1.1 3,297,934 - GFF3241 0.76 -0.9 3,297,952 + GFF3241 0.77 -1.1 3,297,974 + GFF3241 0.79 -1.5 3,297,987 + GFF3241 0.80 -1.5 3,297,987 + GFF3241 0.80 -1.2 3,297,987 + GFF3241 0.80 -0.8 3,297,994 + GFF3241 0.81 -1.9 3,297,994 + GFF3241 0.81 -1.1 3,297,994 + GFF3241 0.81 -1.6 3,297,995 - GFF3241 0.81 -1.5 3,297,995 - GFF3241 0.81 -1.1 3,297,995 - GFF3241 0.81 -0.8 3,297,995 - GFF3241 0.81 -1.0 3,298,002 - GFF3241 0.81 -0.6 3,298,002 - GFF3241 0.81 -1.5 3,298,007 - GFF3241 0.82 -1.7 3,298,056 + GFF3241 0.86 -0.6 3,298,078 - GFF3241 0.87 -1.7 3,298,091 - GFF3241 0.88 -1.9 3,298,097 + GFF3241 0.89 -0.9 3,298,123 + -1.0 3,298,123 + -2.5 3,298,131 - -2.3 3,298,133 - -1.6 3,298,135 + -0.9 3,298,135 + +0.4 3,298,135 + -1.1 3,298,143 - -1.2 3,298,143 - -1.8 3,298,143 - -1.8 3,298,155 + -1.3 3,298,190 - -0.5 3,298,228 + -1.1 3,298,248 + -1.1 3,298,256 + -0.9 3,298,256 - -0.7 3,298,256 - -2.1 3,298,257 - -0.4 3,298,268 + -0.9 3,298,268 + -1.0 3,298,268 + -1.1 3,298,268 + -1.1 3,298,268 + -0.6 3,298,268 + -1.2 3,298,276 - -1.1 3,298,276 - -3.1 3,298,276 - -1.3 3,298,276 - -1.6 3,298,303 + GFF3242 0.11 -0.6 3,298,303 + GFF3242 0.11 +1.4 3,298,311 - GFF3242 0.12 -1.5 3,298,311 - GFF3242 0.12 -0.8 3,298,323 + GFF3242 0.14 -1.2 3,298,343 + GFF3242 0.17 -0.7 3,298,366 - GFF3242 0.20 -0.8 3,298,376 + GFF3242 0.22 -0.8 3,298,376 + GFF3242 0.22 -1.8 3,298,399 - GFF3242 0.25 -1.6 3,298,433 - GFF3242 0.30 -1.9 3,298,544 - GFF3242 0.47 -0.6 3,298,551 - GFF3242 0.48 -1.0 3,298,551 - GFF3242 0.48 -0.6 3,298,565 + GFF3242 0.50 -1.9 3,298,618 - GFF3242 0.58 -0.7 3,298,620 - GFF3242 0.59 -1.4 3,298,620 - GFF3242 0.59 -0.9 3,298,662 + GFF3242 0.65 -0.6 3,298,667 + GFF3242 0.66 -0.6 3,298,670 - GFF3242 0.66 -1.6 3,298,670 - GFF3242 0.66 -1.4 3,298,676 + GFF3242 0.67 -1.6 3,298,676 + GFF3242 0.67 -3.6 3,298,681 + GFF3242 0.68 -0.9 3,298,681 + GFF3242 0.68 -0.2 3,298,681 + GFF3242 0.68 -1.4 3,298,684 - GFF3242 0.68 -0.6 3,298,689 - GFF3242 0.69 -1.5 3,298,689 - GFF3242 0.69 -3.7 3,298,689 - GFF3242 0.69 -1.5 3,298,689 - GFF3242 0.69 -1.4 3,298,689 - GFF3242 0.69 -1.2 3,298,689 - GFF3242 0.69 -2.5 3,298,694 - GFF3242 0.70 -1.7 3,298,694 - GFF3242 0.70 -0.7 3,298,701 - GFF3242 0.71 -1.4 3,298,716 + GFF3242 0.73 -1.1 3,298,716 + GFF3242 0.73 -0.8 3,298,716 + GFF3242 0.73 -0.8 3,298,724 - GFF3242 0.74 -0.7 3,298,773 + GFF3242 0.82 -1.1 3,298,815 - GFF3242 0.88 -1.7 3,298,829 + -1.5 3,298,829 + +0.8 3,298,842 - -2.0 3,298,845 + -2.0 3,298,853 + -1.5 3,298,860 - -2.4 3,298,897 - -0.7 3,298,897 - -1.7 3,298,911 - -1.5 3,298,972 - -1.2 3,298,979 + -0.6 3,298,979 + +1.3 3,298,981 + +0.7 3,298,987 - -0.2 3,298,987 - -0.5 3,298,987 - -0.7 3,298,989 - -2.0 3,298,994 + -0.6 3,299,022 - +0.3 3,299,034 - -0.5 3,299,055 + +0.0 3,299,063 - -1.0 3,299,063 - +0.0 3,299,076 + -0.2 3,299,094 - -1.4 3,299,098 + -0.5 3,299,128 - GFF3243 0.11 -1.1 3,299,134 - GFF3243 0.12 -0.6 3,299,135 + GFF3243 0.12 +1.5 3,299,154 + GFF3243 0.13 +0.4 3,299,167 - GFF3243 0.14 +0.1 3,299,174 + GFF3243 0.15 -1.2 3,299,183 - GFF3243 0.15 -0.4
Or see this region's nucleotide sequence