Experiment: EMM_noCarbon with Sucrose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1908 and GFF1909 are separated by 5 nucleotides GFF1909 and GFF1910 are separated by 161 nucleotides
GFF1908 - hypothetical protein, at 1,931,132 to 1,932,106
GFF1908
GFF1909 - Chorismate mutase I (EC 5.4.99.5), at 1,932,112 to 1,932,390
GFF1909
GFF1910 - Decaprenyl diphosphate synthase (EC 2.5.1.91), at 1,932,552 to 1,933,577
GFF1910
Position (kb)
1932
1933 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1931.113 kb on - strand at 1931.130 kb on - strand at 1931.137 kb on - strand at 1931.194 kb on - strand at 1931.199 kb on + strand at 1931.199 kb on + strand at 1931.199 kb on + strand at 1931.207 kb on - strand at 1931.207 kb on - strand at 1931.207 kb on - strand at 1931.260 kb on + strand, within GFF1908 at 1931.260 kb on + strand, within GFF1908 at 1931.260 kb on + strand, within GFF1908 at 1931.260 kb on + strand, within GFF1908 at 1931.260 kb on + strand, within GFF1908 at 1931.260 kb on + strand, within GFF1908 at 1931.260 kb on + strand, within GFF1908 at 1931.260 kb on + strand, within GFF1908 at 1931.268 kb on - strand, within GFF1908 at 1931.268 kb on - strand, within GFF1908 at 1931.268 kb on - strand, within GFF1908 at 1931.268 kb on - strand, within GFF1908 at 1931.268 kb on - strand, within GFF1908 at 1931.268 kb on - strand, within GFF1908 at 1931.268 kb on - strand, within GFF1908 at 1931.268 kb on - strand, within GFF1908 at 1931.268 kb on - strand, within GFF1908 at 1931.273 kb on - strand, within GFF1908 at 1931.301 kb on + strand, within GFF1908 at 1931.323 kb on + strand, within GFF1908 at 1931.331 kb on - strand, within GFF1908 at 1931.331 kb on - strand, within GFF1908 at 1931.331 kb on - strand, within GFF1908 at 1931.344 kb on - strand, within GFF1908 at 1931.344 kb on - strand, within GFF1908 at 1931.349 kb on + strand, within GFF1908 at 1931.356 kb on + strand, within GFF1908 at 1931.359 kb on - strand, within GFF1908 at 1931.359 kb on - strand, within GFF1908 at 1931.372 kb on - strand, within GFF1908 at 1931.382 kb on - strand, within GFF1908 at 1931.438 kb on + strand, within GFF1908 at 1931.438 kb on + strand, within GFF1908 at 1931.438 kb on + strand, within GFF1908 at 1931.438 kb on + strand, within GFF1908 at 1931.446 kb on - strand, within GFF1908 at 1931.446 kb on - strand, within GFF1908 at 1931.446 kb on - strand, within GFF1908 at 1931.446 kb on - strand, within GFF1908 at 1931.446 kb on - strand, within GFF1908 at 1931.451 kb on - strand, within GFF1908 at 1931.461 kb on + strand, within GFF1908 at 1931.464 kb on - strand, within GFF1908 at 1931.469 kb on - strand, within GFF1908 at 1931.469 kb on - strand, within GFF1908 at 1931.504 kb on - strand, within GFF1908 at 1931.504 kb on - strand, within GFF1908 at 1931.559 kb on + strand, within GFF1908 at 1931.577 kb on - strand, within GFF1908 at 1931.654 kb on + strand, within GFF1908 at 1931.654 kb on + strand, within GFF1908 at 1931.654 kb on + strand, within GFF1908 at 1931.654 kb on + strand, within GFF1908 at 1931.654 kb on + strand, within GFF1908 at 1931.654 kb on + strand, within GFF1908 at 1931.654 kb on + strand, within GFF1908 at 1931.654 kb on + strand, within GFF1908 at 1931.654 kb on + strand, within GFF1908 at 1931.654 kb on + strand, within GFF1908 at 1931.654 kb on + strand, within GFF1908 at 1931.655 kb on + strand, within GFF1908 at 1931.655 kb on + strand, within GFF1908 at 1931.655 kb on + strand, within GFF1908 at 1931.655 kb on + strand, within GFF1908 at 1931.655 kb on + strand, within GFF1908 at 1931.656 kb on + strand, within GFF1908 at 1931.662 kb on - strand, within GFF1908 at 1931.662 kb on - strand, within GFF1908 at 1931.662 kb on - strand, within GFF1908 at 1931.662 kb on - strand, within GFF1908 at 1931.662 kb on - strand, within GFF1908 at 1931.662 kb on - strand, within GFF1908 at 1931.662 kb on - strand, within GFF1908 at 1931.662 kb on - strand, within GFF1908 at 1931.662 kb on - strand, within GFF1908 at 1931.662 kb on - strand, within GFF1908 at 1931.662 kb on - strand, within GFF1908 at 1931.662 kb on - strand, within GFF1908 at 1931.662 kb on - strand, within GFF1908 at 1931.662 kb on - strand, within GFF1908 at 1931.662 kb on - strand, within GFF1908 at 1931.666 kb on + strand, within GFF1908 at 1931.667 kb on - strand, within GFF1908 at 1931.692 kb on + strand, within GFF1908 at 1931.698 kb on + strand, within GFF1908 at 1931.700 kb on - strand, within GFF1908 at 1931.700 kb on - strand, within GFF1908 at 1931.700 kb on - strand, within GFF1908 at 1931.700 kb on - strand, within GFF1908 at 1931.703 kb on + strand, within GFF1908 at 1931.703 kb on + strand, within GFF1908 at 1931.703 kb on + strand, within GFF1908 at 1931.705 kb on - strand, within GFF1908 at 1931.710 kb on - strand, within GFF1908 at 1931.711 kb on - strand, within GFF1908 at 1931.711 kb on - strand, within GFF1908 at 1931.711 kb on - strand, within GFF1908 at 1931.713 kb on + strand, within GFF1908 at 1931.722 kb on - strand, within GFF1908 at 1931.727 kb on - strand, within GFF1908 at 1931.732 kb on - strand, within GFF1908 at 1931.746 kb on + strand, within GFF1908 at 1931.768 kb on + strand, within GFF1908 at 1931.768 kb on + strand, within GFF1908 at 1931.769 kb on + strand, within GFF1908 at 1931.769 kb on + strand, within GFF1908 at 1931.770 kb on + strand, within GFF1908 at 1931.777 kb on - strand, within GFF1908 at 1931.784 kb on + strand, within GFF1908 at 1931.784 kb on + strand, within GFF1908 at 1931.800 kb on + strand, within GFF1908 at 1931.815 kb on + strand, within GFF1908 at 1931.815 kb on + strand, within GFF1908 at 1931.815 kb on + strand, within GFF1908 at 1931.815 kb on + strand, within GFF1908 at 1931.823 kb on - strand, within GFF1908 at 1931.824 kb on + strand, within GFF1908 at 1931.850 kb on - strand, within GFF1908 at 1931.859 kb on + strand, within GFF1908 at 1931.859 kb on + strand, within GFF1908 at 1931.860 kb on - strand, within GFF1908 at 1931.867 kb on - strand, within GFF1908 at 1931.867 kb on - strand, within GFF1908 at 1931.885 kb on + strand, within GFF1908 at 1931.885 kb on + strand, within GFF1908 at 1931.885 kb on + strand, within GFF1908 at 1931.893 kb on - strand, within GFF1908 at 1931.893 kb on - strand, within GFF1908 at 1931.893 kb on - strand, within GFF1908 at 1931.893 kb on - strand, within GFF1908 at 1931.893 kb on - strand, within GFF1908 at 1931.951 kb on + strand, within GFF1908 at 1931.951 kb on + strand, within GFF1908 at 1931.951 kb on + strand, within GFF1908 at 1931.956 kb on + strand, within GFF1908 at 1931.956 kb on + strand, within GFF1908 at 1931.956 kb on + strand, within GFF1908 at 1931.959 kb on - strand, within GFF1908 at 1931.964 kb on - strand, within GFF1908 at 1931.966 kb on - strand, within GFF1908 at 1931.966 kb on - strand, within GFF1908 at 1932.022 kb on - strand at 1932.023 kb on - strand at 1932.039 kb on + strand at 1932.044 kb on + strand at 1932.049 kb on + strand at 1932.052 kb on - strand at 1932.052 kb on - strand at 1932.052 kb on - strand at 1932.052 kb on - strand at 1932.052 kb on - strand at 1932.057 kb on - strand at 1932.059 kb on + strand at 1932.067 kb on - strand at 1932.067 kb on - strand at 1932.067 kb on - strand at 1932.067 kb on - strand at 1932.067 kb on - strand at 1932.069 kb on + strand at 1932.069 kb on + strand at 1932.070 kb on + strand at 1932.070 kb on + strand at 1932.077 kb on - strand at 1932.078 kb on - strand at 1932.078 kb on - strand at 1932.078 kb on - strand at 1932.078 kb on - strand at 1932.109 kb on + strand at 1932.109 kb on + strand at 1932.116 kb on - strand at 1932.117 kb on - strand at 1932.117 kb on - strand at 1932.117 kb on - strand at 1932.118 kb on - strand at 1932.119 kb on + strand at 1932.127 kb on - strand at 1932.129 kb on - strand at 1932.145 kb on - strand, within GFF1909 at 1932.255 kb on - strand, within GFF1909 at 1932.265 kb on - strand, within GFF1909 at 1932.278 kb on + strand, within GFF1909 at 1932.296 kb on + strand, within GFF1909 at 1932.300 kb on + strand, within GFF1909 at 1932.305 kb on + strand, within GFF1909 at 1932.305 kb on + strand, within GFF1909 at 1932.305 kb on + strand, within GFF1909 at 1932.305 kb on + strand, within GFF1909 at 1932.305 kb on + strand, within GFF1909 at 1932.305 kb on + strand, within GFF1909 at 1932.305 kb on + strand, within GFF1909 at 1932.305 kb on + strand, within GFF1909 at 1932.305 kb on + strand, within GFF1909 at 1932.305 kb on + strand, within GFF1909 at 1932.305 kb on + strand, within GFF1909 at 1932.305 kb on + strand, within GFF1909 at 1932.305 kb on + strand, within GFF1909 at 1932.305 kb on + strand, within GFF1909 at 1932.305 kb on + strand, within GFF1909 at 1932.305 kb on + strand, within GFF1909 at 1932.305 kb on + strand, within GFF1909 at 1932.305 kb on + strand, within GFF1909 at 1932.305 kb on + strand, within GFF1909 at 1932.305 kb on + strand, within GFF1909 at 1932.305 kb on + strand, within GFF1909 at 1932.313 kb on - strand, within GFF1909 at 1932.313 kb on - strand, within GFF1909 at 1932.313 kb on - strand, within GFF1909 at 1932.313 kb on - strand, within GFF1909 at 1932.313 kb on - strand, within GFF1909 at 1932.313 kb on - strand, within GFF1909 at 1932.313 kb on - strand, within GFF1909 at 1932.313 kb on - strand, within GFF1909 at 1932.313 kb on - strand, within GFF1909 at 1932.313 kb on - strand, within GFF1909 at 1932.313 kb on - strand, within GFF1909 at 1932.313 kb on - strand, within GFF1909 at 1932.313 kb on - strand, within GFF1909 at 1932.313 kb on - strand, within GFF1909 at 1932.313 kb on - strand, within GFF1909 at 1932.313 kb on - strand, within GFF1909 at 1932.313 kb on - strand, within GFF1909 at 1932.313 kb on - strand, within GFF1909 at 1932.313 kb on - strand, within GFF1909 at 1932.313 kb on - strand, within GFF1909 at 1932.313 kb on - strand, within GFF1909 at 1932.314 kb on - strand, within GFF1909 at 1932.314 kb on - strand, within GFF1909 at 1932.324 kb on + strand, within GFF1909 at 1932.325 kb on + strand, within GFF1909 at 1932.331 kb on + strand, within GFF1909 at 1932.332 kb on - strand, within GFF1909 at 1932.359 kb on - strand, within GFF1909 at 1932.371 kb on - strand at 1932.373 kb on + strand at 1932.402 kb on - strand at 1932.404 kb on - strand at 1932.406 kb on - strand at 1932.407 kb on - strand at 1932.498 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Ammonium Sulfate remove 1,931,113 - -0.6 1,931,130 - +0.3 1,931,137 - -2.4 1,931,194 - +0.3 1,931,199 + +0.3 1,931,199 + -0.2 1,931,199 + +0.2 1,931,207 - -0.4 1,931,207 - -0.6 1,931,207 - -0.9 1,931,260 + GFF1908 0.13 -0.4 1,931,260 + GFF1908 0.13 +0.9 1,931,260 + GFF1908 0.13 -0.3 1,931,260 + GFF1908 0.13 +0.2 1,931,260 + GFF1908 0.13 +0.2 1,931,260 + GFF1908 0.13 -0.4 1,931,260 + GFF1908 0.13 -1.6 1,931,260 + GFF1908 0.13 -0.5 1,931,268 - GFF1908 0.14 -0.1 1,931,268 - GFF1908 0.14 -1.1 1,931,268 - GFF1908 0.14 -0.0 1,931,268 - GFF1908 0.14 -1.5 1,931,268 - GFF1908 0.14 +0.7 1,931,268 - GFF1908 0.14 +1.0 1,931,268 - GFF1908 0.14 +0.9 1,931,268 - GFF1908 0.14 -0.9 1,931,268 - GFF1908 0.14 +0.3 1,931,273 - GFF1908 0.14 -0.3 1,931,301 + GFF1908 0.17 +0.1 1,931,323 + GFF1908 0.20 -1.4 1,931,331 - GFF1908 0.20 +0.3 1,931,331 - GFF1908 0.20 -1.7 1,931,331 - GFF1908 0.20 +1.2 1,931,344 - GFF1908 0.22 -0.3 1,931,344 - GFF1908 0.22 -0.5 1,931,349 + GFF1908 0.22 -0.4 1,931,356 + GFF1908 0.23 -0.7 1,931,359 - GFF1908 0.23 -1.1 1,931,359 - GFF1908 0.23 -0.3 1,931,372 - GFF1908 0.25 +0.1 1,931,382 - GFF1908 0.26 -2.2 1,931,438 + GFF1908 0.31 -1.5 1,931,438 + GFF1908 0.31 -1.5 1,931,438 + GFF1908 0.31 +1.0 1,931,438 + GFF1908 0.31 +0.6 1,931,446 - GFF1908 0.32 +0.6 1,931,446 - GFF1908 0.32 +0.1 1,931,446 - GFF1908 0.32 +0.0 1,931,446 - GFF1908 0.32 -0.7 1,931,446 - GFF1908 0.32 +0.1 1,931,451 - GFF1908 0.33 -1.7 1,931,461 + GFF1908 0.34 +0.7 1,931,464 - GFF1908 0.34 -0.3 1,931,469 - GFF1908 0.35 +0.1 1,931,469 - GFF1908 0.35 +0.1 1,931,504 - GFF1908 0.38 -2.0 1,931,504 - GFF1908 0.38 -1.3 1,931,559 + GFF1908 0.44 -0.4 1,931,577 - GFF1908 0.46 -0.7 1,931,654 + GFF1908 0.54 -1.0 1,931,654 + GFF1908 0.54 -0.4 1,931,654 + GFF1908 0.54 -1.5 1,931,654 + GFF1908 0.54 -0.6 1,931,654 + GFF1908 0.54 -1.1 1,931,654 + GFF1908 0.54 +1.3 1,931,654 + GFF1908 0.54 -1.0 1,931,654 + GFF1908 0.54 -0.7 1,931,654 + GFF1908 0.54 +0.6 1,931,654 + GFF1908 0.54 +0.5 1,931,654 + GFF1908 0.54 -0.8 1,931,655 + GFF1908 0.54 +0.1 1,931,655 + GFF1908 0.54 -0.5 1,931,655 + GFF1908 0.54 +0.3 1,931,655 + GFF1908 0.54 +0.2 1,931,655 + GFF1908 0.54 -0.5 1,931,656 + GFF1908 0.54 +0.5 1,931,662 - GFF1908 0.54 -0.4 1,931,662 - GFF1908 0.54 -1.1 1,931,662 - GFF1908 0.54 -0.2 1,931,662 - GFF1908 0.54 +1.5 1,931,662 - GFF1908 0.54 -0.9 1,931,662 - GFF1908 0.54 -0.4 1,931,662 - GFF1908 0.54 -0.3 1,931,662 - GFF1908 0.54 -0.8 1,931,662 - GFF1908 0.54 -2.1 1,931,662 - GFF1908 0.54 -1.0 1,931,662 - GFF1908 0.54 -1.9 1,931,662 - GFF1908 0.54 -0.1 1,931,662 - GFF1908 0.54 +0.5 1,931,662 - GFF1908 0.54 -0.5 1,931,662 - GFF1908 0.54 +1.5 1,931,666 + GFF1908 0.55 -1.1 1,931,667 - GFF1908 0.55 -1.1 1,931,692 + GFF1908 0.57 -0.1 1,931,698 + GFF1908 0.58 -1.0 1,931,700 - GFF1908 0.58 -1.0 1,931,700 - GFF1908 0.58 -0.1 1,931,700 - GFF1908 0.58 -0.5 1,931,700 - GFF1908 0.58 -0.7 1,931,703 + GFF1908 0.59 -0.1 1,931,703 + GFF1908 0.59 -1.1 1,931,703 + GFF1908 0.59 -2.0 1,931,705 - GFF1908 0.59 +0.4 1,931,710 - GFF1908 0.59 -0.1 1,931,711 - GFF1908 0.59 -1.3 1,931,711 - GFF1908 0.59 +0.7 1,931,711 - GFF1908 0.59 -0.8 1,931,713 + GFF1908 0.60 -0.3 1,931,722 - GFF1908 0.61 -1.1 1,931,727 - GFF1908 0.61 -0.6 1,931,732 - GFF1908 0.62 -1.8 1,931,746 + GFF1908 0.63 -0.6 1,931,768 + GFF1908 0.65 -0.7 1,931,768 + GFF1908 0.65 +1.5 1,931,769 + GFF1908 0.65 -0.5 1,931,769 + GFF1908 0.65 +0.0 1,931,770 + GFF1908 0.65 -0.4 1,931,777 - GFF1908 0.66 +0.8 1,931,784 + GFF1908 0.67 -0.3 1,931,784 + GFF1908 0.67 -0.9 1,931,800 + GFF1908 0.69 +0.4 1,931,815 + GFF1908 0.70 -0.9 1,931,815 + GFF1908 0.70 +2.4 1,931,815 + GFF1908 0.70 +1.1 1,931,815 + GFF1908 0.70 +0.6 1,931,823 - GFF1908 0.71 -2.1 1,931,824 + GFF1908 0.71 -0.0 1,931,850 - GFF1908 0.74 +0.6 1,931,859 + GFF1908 0.75 +0.5 1,931,859 + GFF1908 0.75 +0.3 1,931,860 - GFF1908 0.75 +0.1 1,931,867 - GFF1908 0.75 -0.7 1,931,867 - GFF1908 0.75 -0.3 1,931,885 + GFF1908 0.77 -0.9 1,931,885 + GFF1908 0.77 -0.3 1,931,885 + GFF1908 0.77 +0.8 1,931,893 - GFF1908 0.78 +0.4 1,931,893 - GFF1908 0.78 +0.0 1,931,893 - GFF1908 0.78 -0.9 1,931,893 - GFF1908 0.78 -1.3 1,931,893 - GFF1908 0.78 +0.1 1,931,951 + GFF1908 0.84 -1.3 1,931,951 + GFF1908 0.84 -0.5 1,931,951 + GFF1908 0.84 +0.6 1,931,956 + GFF1908 0.85 -1.1 1,931,956 + GFF1908 0.85 +0.1 1,931,956 + GFF1908 0.85 -0.2 1,931,959 - GFF1908 0.85 +0.4 1,931,964 - GFF1908 0.85 -1.7 1,931,966 - GFF1908 0.86 +0.4 1,931,966 - GFF1908 0.86 -1.0 1,932,022 - -1.2 1,932,023 - +1.1 1,932,039 + -0.3 1,932,044 + -1.5 1,932,049 + +0.8 1,932,052 - +0.9 1,932,052 - +0.9 1,932,052 - +0.0 1,932,052 - -0.8 1,932,052 - +0.1 1,932,057 - +0.3 1,932,059 + -0.3 1,932,067 - +0.1 1,932,067 - +1.0 1,932,067 - -0.6 1,932,067 - +0.2 1,932,067 - +0.3 1,932,069 + -0.6 1,932,069 + +0.2 1,932,070 + -0.4 1,932,070 + -0.4 1,932,077 - +0.5 1,932,078 - -0.8 1,932,078 - -0.1 1,932,078 - +0.1 1,932,078 - +1.2 1,932,109 + +1.2 1,932,109 + +0.0 1,932,116 - -0.4 1,932,117 - -0.8 1,932,117 - -0.8 1,932,117 - -1.3 1,932,118 - +0.6 1,932,119 + -1.8 1,932,127 - -2.4 1,932,129 - -2.1 1,932,145 - GFF1909 0.12 -1.7 1,932,255 - GFF1909 0.51 +0.8 1,932,265 - GFF1909 0.55 -1.9 1,932,278 + GFF1909 0.59 -0.6 1,932,296 + GFF1909 0.66 -1.3 1,932,300 + GFF1909 0.67 -1.3 1,932,305 + GFF1909 0.69 -1.6 1,932,305 + GFF1909 0.69 -1.7 1,932,305 + GFF1909 0.69 -1.8 1,932,305 + GFF1909 0.69 -1.1 1,932,305 + GFF1909 0.69 -2.8 1,932,305 + GFF1909 0.69 -2.5 1,932,305 + GFF1909 0.69 -2.6 1,932,305 + GFF1909 0.69 -0.3 1,932,305 + GFF1909 0.69 -2.1 1,932,305 + GFF1909 0.69 -1.5 1,932,305 + GFF1909 0.69 -0.7 1,932,305 + GFF1909 0.69 -2.0 1,932,305 + GFF1909 0.69 -1.1 1,932,305 + GFF1909 0.69 -3.5 1,932,305 + GFF1909 0.69 -2.9 1,932,305 + GFF1909 0.69 -1.3 1,932,305 + GFF1909 0.69 -0.6 1,932,305 + GFF1909 0.69 -1.8 1,932,305 + GFF1909 0.69 -1.1 1,932,305 + GFF1909 0.69 -2.2 1,932,305 + GFF1909 0.69 -1.3 1,932,313 - GFF1909 0.72 -2.0 1,932,313 - GFF1909 0.72 -1.7 1,932,313 - GFF1909 0.72 -2.9 1,932,313 - GFF1909 0.72 -0.3 1,932,313 - GFF1909 0.72 -2.3 1,932,313 - GFF1909 0.72 -2.9 1,932,313 - GFF1909 0.72 -0.6 1,932,313 - GFF1909 0.72 -2.4 1,932,313 - GFF1909 0.72 -0.8 1,932,313 - GFF1909 0.72 -2.6 1,932,313 - GFF1909 0.72 -2.0 1,932,313 - GFF1909 0.72 -2.4 1,932,313 - GFF1909 0.72 -1.2 1,932,313 - GFF1909 0.72 -3.2 1,932,313 - GFF1909 0.72 -3.2 1,932,313 - GFF1909 0.72 -3.2 1,932,313 - GFF1909 0.72 -1.7 1,932,313 - GFF1909 0.72 -1.7 1,932,313 - GFF1909 0.72 -2.6 1,932,313 - GFF1909 0.72 -2.2 1,932,313 - GFF1909 0.72 -1.8 1,932,314 - GFF1909 0.72 -1.5 1,932,314 - GFF1909 0.72 -2.5 1,932,324 + GFF1909 0.76 -0.6 1,932,325 + GFF1909 0.76 -1.2 1,932,331 + GFF1909 0.78 -1.9 1,932,332 - GFF1909 0.79 -0.4 1,932,359 - GFF1909 0.89 -2.6 1,932,371 - -1.8 1,932,373 + -0.4 1,932,402 - -0.1 1,932,404 - +0.1 1,932,406 - -0.4 1,932,407 - -1.1 1,932,498 + -0.6
Or see this region's nucleotide sequence