Experiment: EMM_noCarbon with Sucrose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1852 and GFF1853 are separated by 2 nucleotides GFF1853 and GFF1854 are separated by 2 nucleotides GFF1854 and GFF1855 overlap by 4 nucleotides
GFF1852 - Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolyzing) (EC 6.3.5.9), at 1,882,582 to 1,883,880
GFF1852
GFF1853 - Precorrin-4 C(11)-methyltransferase (EC 2.1.1.133), at 1,883,883 to 1,884,668
GFF1853
GFF1854 - Cobalamin biosynthesis protein CobE, at 1,884,671 to 1,885,027
GFF1854
GFF1855 - Precorrin-6Y C(5,15)-methyltransferase [decarboxylating] (EC 2.1.1.132), at 1,885,024 to 1,886,175
GFF1855
Position (kb)
1884
1885
1886 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1883.671 kb on + strand, within GFF1852 at 1883.671 kb on + strand, within GFF1852 at 1883.679 kb on - strand, within GFF1852 at 1883.679 kb on - strand, within GFF1852 at 1883.679 kb on - strand, within GFF1852 at 1883.679 kb on - strand, within GFF1852 at 1883.679 kb on - strand, within GFF1852 at 1883.688 kb on + strand, within GFF1852 at 1883.706 kb on + strand, within GFF1852 at 1883.748 kb on + strand, within GFF1852 at 1883.753 kb on + strand at 1883.756 kb on - strand at 1883.776 kb on + strand at 1883.785 kb on + strand at 1883.789 kb on - strand at 1883.790 kb on + strand at 1883.841 kb on - strand at 1883.902 kb on - strand at 1883.902 kb on - strand at 1883.921 kb on - strand at 1883.936 kb on + strand at 1883.938 kb on + strand at 1883.938 kb on + strand at 1883.938 kb on + strand at 1883.938 kb on + strand at 1883.944 kb on - strand at 1883.946 kb on - strand at 1883.984 kb on + strand, within GFF1853 at 1884.017 kb on + strand, within GFF1853 at 1884.017 kb on + strand, within GFF1853 at 1884.017 kb on + strand, within GFF1853 at 1884.017 kb on + strand, within GFF1853 at 1884.017 kb on + strand, within GFF1853 at 1884.025 kb on - strand, within GFF1853 at 1884.025 kb on - strand, within GFF1853 at 1884.025 kb on - strand, within GFF1853 at 1884.025 kb on - strand, within GFF1853 at 1884.040 kb on + strand, within GFF1853 at 1884.077 kb on + strand, within GFF1853 at 1884.085 kb on - strand, within GFF1853 at 1884.112 kb on + strand, within GFF1853 at 1884.139 kb on + strand, within GFF1853 at 1884.145 kb on + strand, within GFF1853 at 1884.153 kb on - strand, within GFF1853 at 1884.153 kb on - strand, within GFF1853 at 1884.183 kb on + strand, within GFF1853 at 1884.188 kb on + strand, within GFF1853 at 1884.238 kb on + strand, within GFF1853 at 1884.238 kb on + strand, within GFF1853 at 1884.238 kb on + strand, within GFF1853 at 1884.238 kb on + strand, within GFF1853 at 1884.238 kb on + strand, within GFF1853 at 1884.238 kb on + strand, within GFF1853 at 1884.246 kb on - strand, within GFF1853 at 1884.246 kb on - strand, within GFF1853 at 1884.246 kb on - strand, within GFF1853 at 1884.246 kb on - strand, within GFF1853 at 1884.246 kb on - strand, within GFF1853 at 1884.248 kb on + strand, within GFF1853 at 1884.279 kb on + strand, within GFF1853 at 1884.283 kb on + strand, within GFF1853 at 1884.292 kb on - strand, within GFF1853 at 1884.299 kb on + strand, within GFF1853 at 1884.299 kb on + strand, within GFF1853 at 1884.301 kb on - strand, within GFF1853 at 1884.314 kb on - strand, within GFF1853 at 1884.348 kb on + strand, within GFF1853 at 1884.374 kb on + strand, within GFF1853 at 1884.413 kb on + strand, within GFF1853 at 1884.437 kb on + strand, within GFF1853 at 1884.487 kb on + strand, within GFF1853 at 1884.494 kb on + strand, within GFF1853 at 1884.508 kb on + strand, within GFF1853 at 1884.508 kb on + strand, within GFF1853 at 1884.516 kb on - strand, within GFF1853 at 1884.516 kb on - strand, within GFF1853 at 1884.516 kb on - strand, within GFF1853 at 1884.541 kb on - strand, within GFF1853 at 1884.556 kb on - strand, within GFF1853 at 1884.556 kb on - strand, within GFF1853 at 1884.579 kb on - strand, within GFF1853 at 1884.579 kb on - strand, within GFF1853 at 1884.618 kb on + strand at 1884.677 kb on + strand at 1884.678 kb on + strand at 1884.678 kb on + strand at 1884.678 kb on - strand at 1884.686 kb on - strand at 1884.727 kb on - strand, within GFF1854 at 1884.744 kb on - strand, within GFF1854 at 1884.756 kb on - strand, within GFF1854 at 1884.765 kb on + strand, within GFF1854 at 1884.765 kb on + strand, within GFF1854 at 1884.766 kb on - strand, within GFF1854 at 1884.771 kb on - strand, within GFF1854 at 1884.773 kb on - strand, within GFF1854 at 1884.778 kb on - strand, within GFF1854 at 1884.778 kb on - strand, within GFF1854 at 1884.778 kb on - strand, within GFF1854 at 1884.783 kb on - strand, within GFF1854 at 1884.783 kb on - strand, within GFF1854 at 1884.784 kb on + strand, within GFF1854 at 1884.828 kb on - strand, within GFF1854 at 1884.828 kb on - strand, within GFF1854 at 1884.829 kb on - strand, within GFF1854 at 1884.911 kb on - strand, within GFF1854 at 1884.911 kb on - strand, within GFF1854 at 1884.911 kb on - strand, within GFF1854 at 1884.917 kb on - strand, within GFF1854 at 1884.935 kb on + strand, within GFF1854 at 1884.957 kb on + strand, within GFF1854 at 1884.970 kb on - strand, within GFF1854 at 1884.979 kb on + strand, within GFF1854 at 1884.987 kb on - strand, within GFF1854 at 1884.987 kb on - strand, within GFF1854 at 1885.034 kb on + strand at 1885.037 kb on - strand at 1885.039 kb on + strand at 1885.047 kb on + strand at 1885.047 kb on + strand at 1885.047 kb on - strand at 1885.052 kb on - strand at 1885.055 kb on - strand at 1885.077 kb on - strand at 1885.094 kb on + strand at 1885.102 kb on - strand at 1885.113 kb on + strand at 1885.113 kb on + strand at 1885.129 kb on - strand at 1885.134 kb on + strand at 1885.142 kb on - strand, within GFF1855 at 1885.142 kb on - strand, within GFF1855 at 1885.164 kb on + strand, within GFF1855 at 1885.191 kb on + strand, within GFF1855 at 1885.191 kb on + strand, within GFF1855 at 1885.191 kb on + strand, within GFF1855 at 1885.199 kb on - strand, within GFF1855 at 1885.222 kb on - strand, within GFF1855 at 1885.224 kb on - strand, within GFF1855 at 1885.235 kb on + strand, within GFF1855 at 1885.253 kb on - strand, within GFF1855 at 1885.286 kb on + strand, within GFF1855 at 1885.299 kb on + strand, within GFF1855 at 1885.338 kb on + strand, within GFF1855 at 1885.346 kb on - strand, within GFF1855 at 1885.357 kb on + strand, within GFF1855 at 1885.372 kb on + strand, within GFF1855 at 1885.372 kb on + strand, within GFF1855 at 1885.376 kb on + strand, within GFF1855 at 1885.380 kb on - strand, within GFF1855 at 1885.381 kb on + strand, within GFF1855 at 1885.381 kb on + strand, within GFF1855 at 1885.381 kb on + strand, within GFF1855 at 1885.389 kb on - strand, within GFF1855 at 1885.389 kb on - strand, within GFF1855 at 1885.404 kb on - strand, within GFF1855 at 1885.469 kb on + strand, within GFF1855 at 1885.500 kb on + strand, within GFF1855 at 1885.500 kb on + strand, within GFF1855 at 1885.500 kb on + strand, within GFF1855 at 1885.508 kb on - strand, within GFF1855 at 1885.508 kb on - strand, within GFF1855 at 1885.508 kb on - strand, within GFF1855 at 1885.508 kb on - strand, within GFF1855 at 1885.539 kb on - strand, within GFF1855 at 1885.543 kb on + strand, within GFF1855 at 1885.576 kb on + strand, within GFF1855 at 1885.576 kb on + strand, within GFF1855 at 1885.576 kb on + strand, within GFF1855 at 1885.581 kb on + strand, within GFF1855 at 1885.581 kb on + strand, within GFF1855 at 1885.581 kb on + strand, within GFF1855 at 1885.581 kb on + strand, within GFF1855 at 1885.584 kb on - strand, within GFF1855 at 1885.584 kb on - strand, within GFF1855 at 1885.584 kb on - strand, within GFF1855 at 1885.587 kb on + strand, within GFF1855 at 1885.589 kb on - strand, within GFF1855 at 1885.589 kb on - strand, within GFF1855 at 1885.589 kb on - strand, within GFF1855 at 1885.589 kb on - strand, within GFF1855 at 1885.589 kb on - strand, within GFF1855 at 1885.602 kb on + strand, within GFF1855 at 1885.602 kb on + strand, within GFF1855 at 1885.602 kb on + strand, within GFF1855 at 1885.602 kb on + strand, within GFF1855 at 1885.602 kb on + strand, within GFF1855 at 1885.602 kb on + strand, within GFF1855 at 1885.602 kb on + strand, within GFF1855 at 1885.610 kb on - strand, within GFF1855 at 1885.610 kb on - strand, within GFF1855 at 1885.610 kb on - strand, within GFF1855 at 1885.610 kb on - strand, within GFF1855 at 1885.610 kb on - strand, within GFF1855 at 1885.610 kb on - strand, within GFF1855 at 1885.610 kb on - strand, within GFF1855 at 1885.668 kb on + strand, within GFF1855 at 1885.687 kb on + strand, within GFF1855 at 1885.694 kb on - strand, within GFF1855 at 1885.696 kb on + strand, within GFF1855 at 1885.733 kb on + strand, within GFF1855 at 1885.823 kb on + strand, within GFF1855 at 1885.845 kb on + strand, within GFF1855 at 1885.861 kb on + strand, within GFF1855 at 1885.871 kb on + strand, within GFF1855 at 1885.871 kb on + strand, within GFF1855 at 1885.903 kb on + strand, within GFF1855 at 1885.904 kb on - strand, within GFF1855 at 1885.911 kb on - strand, within GFF1855 at 1885.911 kb on - strand, within GFF1855 at 1885.912 kb on - strand, within GFF1855 at 1885.927 kb on + strand, within GFF1855 at 1885.935 kb on - strand, within GFF1855 at 1885.957 kb on + strand, within GFF1855 at 1885.982 kb on + strand, within GFF1855 at 1885.990 kb on - strand, within GFF1855 at 1885.990 kb on - strand, within GFF1855 at 1885.992 kb on + strand, within GFF1855 at 1885.997 kb on + strand, within GFF1855 at 1886.013 kb on + strand, within GFF1855
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Ammonium Sulfate remove 1,883,671 + GFF1852 0.84 -0.6 1,883,671 + GFF1852 0.84 -0.3 1,883,679 - GFF1852 0.84 -0.4 1,883,679 - GFF1852 0.84 -1.9 1,883,679 - GFF1852 0.84 -1.6 1,883,679 - GFF1852 0.84 -0.7 1,883,679 - GFF1852 0.84 -1.8 1,883,688 + GFF1852 0.85 -0.3 1,883,706 + GFF1852 0.87 -1.1 1,883,748 + GFF1852 0.90 -0.3 1,883,753 + -0.9 1,883,756 - -0.8 1,883,776 + -1.8 1,883,785 + -0.5 1,883,789 - -0.9 1,883,790 + -0.3 1,883,841 - -0.5 1,883,902 - -1.5 1,883,902 - +0.5 1,883,921 - -0.5 1,883,936 + -0.7 1,883,938 + -0.4 1,883,938 + -1.5 1,883,938 + -1.4 1,883,938 + -0.3 1,883,944 - -0.9 1,883,946 - -1.2 1,883,984 + GFF1853 0.13 -1.2 1,884,017 + GFF1853 0.17 -1.1 1,884,017 + GFF1853 0.17 -0.3 1,884,017 + GFF1853 0.17 -3.3 1,884,017 + GFF1853 0.17 -0.4 1,884,017 + GFF1853 0.17 -2.5 1,884,025 - GFF1853 0.18 -1.1 1,884,025 - GFF1853 0.18 -1.2 1,884,025 - GFF1853 0.18 -1.1 1,884,025 - GFF1853 0.18 -0.7 1,884,040 + GFF1853 0.20 -0.7 1,884,077 + GFF1853 0.25 -0.3 1,884,085 - GFF1853 0.26 -0.5 1,884,112 + GFF1853 0.29 -0.4 1,884,139 + GFF1853 0.33 -0.5 1,884,145 + GFF1853 0.33 -0.8 1,884,153 - GFF1853 0.34 -0.5 1,884,153 - GFF1853 0.34 -0.5 1,884,183 + GFF1853 0.38 -0.4 1,884,188 + GFF1853 0.39 -0.3 1,884,238 + GFF1853 0.45 -0.7 1,884,238 + GFF1853 0.45 -1.1 1,884,238 + GFF1853 0.45 -0.9 1,884,238 + GFF1853 0.45 -1.0 1,884,238 + GFF1853 0.45 -0.3 1,884,238 + GFF1853 0.45 -1.0 1,884,246 - GFF1853 0.46 -1.4 1,884,246 - GFF1853 0.46 +0.2 1,884,246 - GFF1853 0.46 -1.9 1,884,246 - GFF1853 0.46 -1.1 1,884,246 - GFF1853 0.46 -0.6 1,884,248 + GFF1853 0.46 -0.6 1,884,279 + GFF1853 0.50 -0.8 1,884,283 + GFF1853 0.51 -0.7 1,884,292 - GFF1853 0.52 -0.4 1,884,299 + GFF1853 0.53 -0.3 1,884,299 + GFF1853 0.53 -0.6 1,884,301 - GFF1853 0.53 -1.3 1,884,314 - GFF1853 0.55 -0.8 1,884,348 + GFF1853 0.59 -0.3 1,884,374 + GFF1853 0.62 -1.9 1,884,413 + GFF1853 0.67 +0.2 1,884,437 + GFF1853 0.70 -1.1 1,884,487 + GFF1853 0.77 -1.0 1,884,494 + GFF1853 0.78 -0.5 1,884,508 + GFF1853 0.80 -1.3 1,884,508 + GFF1853 0.80 -0.3 1,884,516 - GFF1853 0.81 -0.6 1,884,516 - GFF1853 0.81 -1.0 1,884,516 - GFF1853 0.81 -0.8 1,884,541 - GFF1853 0.84 -1.2 1,884,556 - GFF1853 0.86 -0.6 1,884,556 - GFF1853 0.86 -0.8 1,884,579 - GFF1853 0.89 -0.4 1,884,579 - GFF1853 0.89 -0.8 1,884,618 + +0.4 1,884,677 + -0.9 1,884,678 + -1.5 1,884,678 + +0.4 1,884,678 - -0.6 1,884,686 - -1.2 1,884,727 - GFF1854 0.16 -1.3 1,884,744 - GFF1854 0.20 -1.9 1,884,756 - GFF1854 0.24 -1.2 1,884,765 + GFF1854 0.26 -0.8 1,884,765 + GFF1854 0.26 -0.6 1,884,766 - GFF1854 0.27 -0.2 1,884,771 - GFF1854 0.28 -1.2 1,884,773 - GFF1854 0.29 -1.5 1,884,778 - GFF1854 0.30 +0.4 1,884,778 - GFF1854 0.30 -1.4 1,884,778 - GFF1854 0.30 -2.0 1,884,783 - GFF1854 0.31 -0.4 1,884,783 - GFF1854 0.31 -0.2 1,884,784 + GFF1854 0.32 -1.0 1,884,828 - GFF1854 0.44 -1.0 1,884,828 - GFF1854 0.44 -0.4 1,884,829 - GFF1854 0.44 -1.4 1,884,911 - GFF1854 0.67 -1.2 1,884,911 - GFF1854 0.67 -0.3 1,884,911 - GFF1854 0.67 -1.2 1,884,917 - GFF1854 0.69 -0.7 1,884,935 + GFF1854 0.74 +1.4 1,884,957 + GFF1854 0.80 -0.7 1,884,970 - GFF1854 0.84 -0.5 1,884,979 + GFF1854 0.86 +0.7 1,884,987 - GFF1854 0.89 -0.2 1,884,987 - GFF1854 0.89 -0.9 1,885,034 + -2.0 1,885,037 - -1.1 1,885,039 + +0.8 1,885,047 + -1.1 1,885,047 + +0.2 1,885,047 - -0.7 1,885,052 - -0.7 1,885,055 - -0.6 1,885,077 - -1.1 1,885,094 + -1.5 1,885,102 - -2.1 1,885,113 + -0.6 1,885,113 + -1.7 1,885,129 - -0.5 1,885,134 + -0.5 1,885,142 - GFF1855 0.10 -0.3 1,885,142 - GFF1855 0.10 -2.0 1,885,164 + GFF1855 0.12 -0.7 1,885,191 + GFF1855 0.14 -0.8 1,885,191 + GFF1855 0.14 +0.4 1,885,191 + GFF1855 0.14 -2.0 1,885,199 - GFF1855 0.15 -1.5 1,885,222 - GFF1855 0.17 -0.3 1,885,224 - GFF1855 0.17 -0.4 1,885,235 + GFF1855 0.18 -1.0 1,885,253 - GFF1855 0.20 -0.6 1,885,286 + GFF1855 0.23 -0.6 1,885,299 + GFF1855 0.24 -0.3 1,885,338 + GFF1855 0.27 +0.2 1,885,346 - GFF1855 0.28 -0.5 1,885,357 + GFF1855 0.29 -0.4 1,885,372 + GFF1855 0.30 -1.3 1,885,372 + GFF1855 0.30 -0.6 1,885,376 + GFF1855 0.31 -0.8 1,885,380 - GFF1855 0.31 -2.1 1,885,381 + GFF1855 0.31 -0.3 1,885,381 + GFF1855 0.31 -1.0 1,885,381 + GFF1855 0.31 -0.5 1,885,389 - GFF1855 0.32 -1.2 1,885,389 - GFF1855 0.32 -1.2 1,885,404 - GFF1855 0.33 -0.3 1,885,469 + GFF1855 0.39 -1.2 1,885,500 + GFF1855 0.41 -1.7 1,885,500 + GFF1855 0.41 -0.3 1,885,500 + GFF1855 0.41 -0.3 1,885,508 - GFF1855 0.42 -0.5 1,885,508 - GFF1855 0.42 -0.4 1,885,508 - GFF1855 0.42 -0.6 1,885,508 - GFF1855 0.42 -1.0 1,885,539 - GFF1855 0.45 -0.8 1,885,543 + GFF1855 0.45 +0.7 1,885,576 + GFF1855 0.48 -0.3 1,885,576 + GFF1855 0.48 -0.5 1,885,576 + GFF1855 0.48 -0.3 1,885,581 + GFF1855 0.48 -0.4 1,885,581 + GFF1855 0.48 -0.3 1,885,581 + GFF1855 0.48 -1.2 1,885,581 + GFF1855 0.48 -0.4 1,885,584 - GFF1855 0.49 -1.0 1,885,584 - GFF1855 0.49 +0.5 1,885,584 - GFF1855 0.49 -1.4 1,885,587 + GFF1855 0.49 -0.5 1,885,589 - GFF1855 0.49 -1.0 1,885,589 - GFF1855 0.49 +0.1 1,885,589 - GFF1855 0.49 -0.6 1,885,589 - GFF1855 0.49 -1.2 1,885,589 - GFF1855 0.49 -1.2 1,885,602 + GFF1855 0.50 -0.5 1,885,602 + GFF1855 0.50 -0.5 1,885,602 + GFF1855 0.50 -0.7 1,885,602 + GFF1855 0.50 -1.6 1,885,602 + GFF1855 0.50 -0.5 1,885,602 + GFF1855 0.50 -1.1 1,885,602 + GFF1855 0.50 -0.7 1,885,610 - GFF1855 0.51 -0.3 1,885,610 - GFF1855 0.51 -1.0 1,885,610 - GFF1855 0.51 -1.4 1,885,610 - GFF1855 0.51 -0.3 1,885,610 - GFF1855 0.51 -0.4 1,885,610 - GFF1855 0.51 -1.5 1,885,610 - GFF1855 0.51 -1.1 1,885,668 + GFF1855 0.56 -0.4 1,885,687 + GFF1855 0.58 +0.9 1,885,694 - GFF1855 0.58 +0.6 1,885,696 + GFF1855 0.58 +0.6 1,885,733 + GFF1855 0.62 -0.4 1,885,823 + GFF1855 0.69 +0.7 1,885,845 + GFF1855 0.71 -0.4 1,885,861 + GFF1855 0.73 -1.4 1,885,871 + GFF1855 0.74 -0.7 1,885,871 + GFF1855 0.74 -0.7 1,885,903 + GFF1855 0.76 -1.1 1,885,904 - GFF1855 0.76 -0.4 1,885,911 - GFF1855 0.77 -1.3 1,885,911 - GFF1855 0.77 -0.4 1,885,912 - GFF1855 0.77 -1.1 1,885,927 + GFF1855 0.78 -1.4 1,885,935 - GFF1855 0.79 -0.7 1,885,957 + GFF1855 0.81 -1.4 1,885,982 + GFF1855 0.83 -0.8 1,885,990 - GFF1855 0.84 -0.8 1,885,990 - GFF1855 0.84 -1.1 1,885,992 + GFF1855 0.84 -1.2 1,885,997 + GFF1855 0.84 -0.4 1,886,013 + GFF1855 0.86 -0.6
Or see this region's nucleotide sequence