Experiment: JK42
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt umuC and dsbB are separated by 45 nucleotides dsbB and nhaB are separated by 145 nucleotides nhaB and fadR are separated by 221 nucleotides fadR and ycgB are separated by 51 nucleotides
OKFHMN_28265: umuC - DNA polymerase V catalytic protein, at 5,419,973 to 5,421,241
umuC
OKFHMN_28270: dsbB - disulfide bond formation protein DsbB, at 5,421,287 to 5,421,817
dsbB
OKFHMN_28275: nhaB - Na(+)/H(+) antiporter NhaB, at 5,421,963 to 5,423,504
nhaB
OKFHMN_28280: fadR - fatty acid metabolism transcriptional regulator FadR, at 5,423,726 to 5,424,445
fadR
OKFHMN_28285: ycgB - SpoVR family protein, at 5,424,497 to 5,426,029
ycgB
Position (kb) 5421
5422
5423
5424 Strain fitness (log2 ratio) -2
-1
0
1 at 5421.361 kb on - strand, within dsbB at 5421.583 kb on - strand, within dsbB at 5421.817 kb on + strand at 5421.943 kb on - strand at 5421.943 kb on - strand at 5421.943 kb on - strand at 5423.170 kb on - strand, within nhaB at 5423.361 kb on + strand at 5423.545 kb on - strand at 5424.240 kb on - strand, within fadR at 5424.443 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction JK42 remove 5,421,361 - dsbB OKFHMN_28270 0.14 -2.7 5,421,583 - dsbB OKFHMN_28270 0.56 -1.0 5,421,817 + -0.9 5,421,943 - +0.7 5,421,943 - -1.8 5,421,943 - -0.9 5,423,170 - nhaB OKFHMN_28275 0.78 -0.1 5,423,361 + +1.3 5,423,545 - -0.3 5,424,240 - fadR OKFHMN_28280 0.71 -1.5 5,424,443 + +1.3
Or see this region's nucleotide sequence