Strain Fitness in Escherichia coli ECRC100 around OKFHMN_28225

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntminC and ycgJ are separated by 519 nucleotidesycgJ and OKFHMN_28225 are separated by 102 nucleotidesOKFHMN_28225 and OKFHMN_28230 are separated by 70 nucleotidesOKFHMN_28230 and ycgL are separated by 11 nucleotidesycgL and ycgM are separated by 71 nucleotides OKFHMN_28215: minC - septum site-determining protein MinC, at 5,414,173 to 5,414,868 minC OKFHMN_28220: ycgJ - Uncharacterized protein YcgJ, at 5,415,388 to 5,415,756 ycgJ OKFHMN_28225: OKFHMN_28225 - inhibitor of g-type lysozyme, at 5,415,859 to 5,416,260 _28225 OKFHMN_28230: OKFHMN_28230 - hypothetical protein, at 5,416,331 to 5,416,489 _28230 OKFHMN_28235: ycgL - Protein YcgL, at 5,416,501 to 5,416,794 ycgL OKFHMN_28240: ycgM - fumarylacetoacetate hydrolase family protein, at 5,416,866 to 5,417,525 ycgM Position (kb) 5415 5416 5417Strain fitness (log2 ratio) -1 0 1 2at 5414.886 kb on - strandat 5414.928 kb on - strandat 5415.064 kb on - strandat 5415.303 kb on + strandat 5415.303 kb on + strandat 5415.303 kb on + strandat 5415.354 kb on + strandat 5415.604 kb on + strand, within ycgJat 5415.864 kb on + strandat 5416.106 kb on + strand, within OKFHMN_28225at 5416.110 kb on + strand, within OKFHMN_28225at 5416.214 kb on - strand, within OKFHMN_28225at 5416.264 kb on + strandat 5416.265 kb on - strandat 5416.305 kb on + strandat 5416.384 kb on + strand, within OKFHMN_28230at 5416.388 kb on + strand, within OKFHMN_28230at 5416.389 kb on - strand, within OKFHMN_28230at 5416.463 kb on - strand, within OKFHMN_28230at 5416.544 kb on - strand, within ycgLat 5416.785 kb on + strandat 5416.869 kb on + strandat 5416.916 kb on + strandat 5416.921 kb on + strandat 5416.937 kb on + strand, within ycgMat 5416.941 kb on + strand, within ycgMat 5416.955 kb on + strand, within ycgM

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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5,414,886 - -0.2
5,414,928 - +1.9
5,415,064 - +2.3
5,415,303 + -0.1
5,415,303 + -0.3
5,415,303 + -0.4
5,415,354 + +1.3
5,415,604 + ycgJ OKFHMN_28220 0.59 -0.5
5,415,864 + -0.5
5,416,106 + OKFHMN_28225 0.61 +0.2
5,416,110 + OKFHMN_28225 0.62 -0.6
5,416,214 - OKFHMN_28225 0.88 +0.3
5,416,264 + -0.2
5,416,265 - -0.1
5,416,305 + +1.7
5,416,384 + OKFHMN_28230 0.33 -0.7
5,416,388 + OKFHMN_28230 0.36 +0.3
5,416,389 - OKFHMN_28230 0.36 +0.8
5,416,463 - OKFHMN_28230 0.83 +0.6
5,416,544 - ycgL OKFHMN_28235 0.15 +0.0
5,416,785 + -0.2
5,416,869 + +0.5
5,416,916 + +0.3
5,416,921 + -0.7
5,416,937 + ycgM OKFHMN_28240 0.11 +0.2
5,416,941 + ycgM OKFHMN_28240 0.11 -0.7
5,416,955 + ycgM OKFHMN_28240 0.13 +1.0

Or see this region's nucleotide sequence