Strain Fitness in Escherichia coli ECRC100 around OKFHMN_28190

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthipB and tnp-IS3 are separated by 173 nucleotidestnp-IS3 and tnp-IS3 overlap by 1 nucleotidestnp-IS3 and OKFHMN_28200 are separated by 83 nucleotides OKFHMN_28185: hipB - transcriptional regulator, at 5,409,930 to 5,410,514 hipB OKFHMN_28190: tnp-IS3 - IS3 family transposase, at 5,410,688 to 5,411,014 tnp-IS3 OKFHMN_28195: tnp-IS3 - IS3 family transposase, at 5,411,014 to 5,411,901 tnp-IS3 OKFHMN_28200: OKFHMN_28200 - autotransporter outer membrane beta-barrel domain-containing protein, at 5,411,985 to 5,412,695 _28200 Position (kb) 5410 5411 5412Strain fitness (log2 ratio) -1 0 1at 5409.736 kb on + strandat 5409.771 kb on + strandat 5409.878 kb on + strandat 5410.103 kb on + strand, within hipBat 5410.180 kb on + strand, within hipBat 5410.482 kb on + strandat 5410.550 kb on + strandat 5410.563 kb on + strandat 5410.563 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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5,409,736 + -1.0
5,409,771 + +1.2
5,409,878 + +0.2
5,410,103 + hipB OKFHMN_28185 0.30 -1.5
5,410,180 + hipB OKFHMN_28185 0.43 -0.8
5,410,482 + -1.0
5,410,550 + +0.4
5,410,563 + -0.7
5,410,563 + -0.8

Or see this region's nucleotide sequence