Strain Fitness in Escherichia coli ECRC100 around OKFHMN_27350

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydhS and ydhR are separated by 125 nucleotidesydhR and Val_trna are separated by 107 nucleotidesVal_trna and Val_trna are separated by 4 nucleotidesVal_trna and ydhQ are separated by 307 nucleotides OKFHMN_27330: ydhS - FAD-NAD(P)-binding protein, at 5,261,974 to 5,263,578 ydhS OKFHMN_27335: ydhR - monooxygenase, at 5,263,704 to 5,264,009 ydhR OKFHMN_27345: Val_trna - tRNA-Val(gac), at 5,264,117 to 5,264,193 _trna OKFHMN_27350: Val_trna - tRNA-Val(gac), at 5,264,198 to 5,264,274 _trna OKFHMN_27355: ydhQ - Uncharacterized protein YdhQ, at 5,264,582 to 5,265,838 ydhQ Position (kb) 5264 5265Strain fitness (log2 ratio) -2 -1 0 1at 5263.377 kb on + strand, within ydhSat 5263.411 kb on - strand, within ydhSat 5263.696 kb on - strandat 5263.940 kb on + strand, within ydhRat 5263.941 kb on - strand, within ydhRat 5263.977 kb on - strand, within ydhRat 5264.034 kb on + strandat 5264.062 kb on - strandat 5264.186 kb on - strandat 5264.186 kb on - strandat 5264.283 kb on - strandat 5264.283 kb on - strandat 5264.283 kb on - strandat 5264.283 kb on - strandat 5264.515 kb on + strandat 5264.515 kb on + strandat 5264.533 kb on + strandat 5264.539 kb on + strandat 5264.824 kb on + strand, within ydhQat 5264.825 kb on - strand, within ydhQat 5264.851 kb on + strand, within ydhQat 5264.950 kb on + strand, within ydhQat 5265.145 kb on + strand, within ydhQat 5265.172 kb on + strand, within ydhQat 5265.251 kb on + strand, within ydhQ

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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5,263,377 + ydhS OKFHMN_27330 0.87 -0.3
5,263,411 - ydhS OKFHMN_27330 0.90 -0.2
5,263,696 - -0.1
5,263,940 + ydhR OKFHMN_27335 0.77 +0.1
5,263,941 - ydhR OKFHMN_27335 0.77 +0.5
5,263,977 - ydhR OKFHMN_27335 0.89 -0.1
5,264,034 + +0.9
5,264,062 - +0.3
5,264,186 - +0.6
5,264,186 - -0.7
5,264,283 - +0.5
5,264,283 - +1.1
5,264,283 - -0.3
5,264,283 - -0.7
5,264,515 + -0.7
5,264,515 + +0.7
5,264,533 + +0.9
5,264,539 + +0.5
5,264,824 + ydhQ OKFHMN_27355 0.19 -1.8
5,264,825 - ydhQ OKFHMN_27355 0.19 -1.0
5,264,851 + ydhQ OKFHMN_27355 0.21 +1.3
5,264,950 + ydhQ OKFHMN_27355 0.29 +0.5
5,265,145 + ydhQ OKFHMN_27355 0.45 -0.3
5,265,172 + ydhQ OKFHMN_27355 0.47 +0.5
5,265,251 + ydhQ OKFHMN_27355 0.53 +1.1

Or see this region's nucleotide sequence