Experiment: JK42
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gAF and yoaF are separated by 182 nucleotides yoaF and yeaO are separated by 21 nucleotides yeaO and nimT are separated by 54 nucleotides nimT and nimR are separated by 99 nucleotides nimR and yeaL overlap by 44 nucleotides
OKFHMN_26660: gAF - GAF domain, at 5,130,251 to 5,131,099
gAF
OKFHMN_26665: yoaF - DUF333 domain-containing protein, at 5,131,282 to 5,131,536
yoaF
OKFHMN_26670: yeaO - Uncharacterized protein YeaO, at 5,131,558 to 5,131,905
yeaO
OKFHMN_26675: nimT - 2-nitroimidazole transporter, at 5,131,960 to 5,133,141
nimT
OKFHMN_26680: nimR - DNA-binding transcriptional regulator NimR, at 5,133,241 to 5,134,059
nimR
OKFHMN_26685: yeaL - UPF0756 membrane protein CKO_01811, at 5,134,016 to 5,134,462
yeaL
Position (kb) 5131
5132
5133
5134 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5131.192 kb on + strand at 5131.262 kb on + strand at 5131.266 kb on + strand at 5131.399 kb on + strand, within yoaF at 5131.566 kb on + strand at 5131.566 kb on + strand at 5131.567 kb on - strand at 5131.567 kb on - strand at 5131.947 kb on - strand at 5132.011 kb on + strand at 5132.307 kb on + strand, within nimT at 5132.459 kb on + strand, within nimT at 5132.675 kb on - strand, within nimT at 5132.780 kb on - strand, within nimT at 5132.833 kb on + strand, within nimT at 5133.377 kb on + strand, within nimR at 5133.465 kb on + strand, within nimR at 5133.866 kb on + strand, within nimR at 5133.963 kb on + strand, within nimR at 5133.963 kb on + strand, within nimR at 5133.969 kb on + strand, within nimR at 5134.002 kb on + strand at 5134.003 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction JK42 remove 5,131,192 + -0.5 5,131,262 + +0.4 5,131,266 + -0.2 5,131,399 + yoaF OKFHMN_26665 0.46 -0.6 5,131,566 + -0.5 5,131,566 + +1.0 5,131,567 - -1.0 5,131,567 - -1.8 5,131,947 - +0.2 5,132,011 + -0.6 5,132,307 + nimT OKFHMN_26675 0.29 +2.0 5,132,459 + nimT OKFHMN_26675 0.42 +0.3 5,132,675 - nimT OKFHMN_26675 0.60 +1.3 5,132,780 - nimT OKFHMN_26675 0.69 +0.7 5,132,833 + nimT OKFHMN_26675 0.74 -0.7 5,133,377 + nimR OKFHMN_26680 0.17 +1.3 5,133,465 + nimR OKFHMN_26680 0.27 -0.3 5,133,866 + nimR OKFHMN_26680 0.76 +1.4 5,133,963 + nimR OKFHMN_26680 0.88 +1.0 5,133,963 + nimR OKFHMN_26680 0.88 +0.9 5,133,969 + nimR OKFHMN_26680 0.89 +1.3 5,134,002 + -1.3 5,134,003 - +0.9
Or see this region's nucleotide sequence