Experiment: JK42
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliR and fliQ are separated by 7 nucleotides fliQ and fliP are separated by 9 nucleotides fliP and fliO overlap by 1 nucleotides fliO and fliN are separated by 2 nucleotides fliN and fliM overlap by 4 nucleotides
OKFHMN_25655: fliR - flagellar type III secretion system protein FliR, at 4,962,900 to 4,963,685
fliR
OKFHMN_25660: fliQ - flagellar biosynthesis protein FliQ, at 4,963,693 to 4,963,962
fliQ
OKFHMN_25665: fliP - flagellar type III secretion system pore protein FliP, at 4,963,972 to 4,964,709
fliP
OKFHMN_25670: fliO - flagellar type III secretion system protein FliO, at 4,964,709 to 4,965,074
fliO
OKFHMN_25675: fliN - flagellar motor switch protein FliN, at 4,965,077 to 4,965,490
fliN
OKFHMN_25680: fliM - flagellar motor switch protein FliM, at 4,965,487 to 4,966,491
fliM
Position (kb) 4963
4964
4965 Strain fitness (log2 ratio) -1
0
1 at 4963.322 kb on + strand, within fliR at 4963.686 kb on - strand at 4963.706 kb on + strand at 4963.707 kb on - strand at 4963.737 kb on - strand, within fliQ at 4964.270 kb on - strand, within fliP at 4964.270 kb on - strand, within fliP at 4965.068 kb on + strand at 4965.139 kb on - strand, within fliN at 4965.665 kb on - strand, within fliM at 4965.683 kb on - strand, within fliM
Per-strain Table
Position Strand Gene LocusTag Fraction JK42 remove 4,963,322 + fliR OKFHMN_25655 0.54 -0.4 4,963,686 - +1.4 4,963,706 + -1.2 4,963,707 - +0.8 4,963,737 - fliQ OKFHMN_25660 0.16 +1.2 4,964,270 - fliP OKFHMN_25665 0.40 -1.2 4,964,270 - fliP OKFHMN_25665 0.40 +0.4 4,965,068 + +0.2 4,965,139 - fliN OKFHMN_25675 0.15 -0.8 4,965,665 - fliM OKFHMN_25680 0.18 -0.5 4,965,683 - fliM OKFHMN_25680 0.20 -1.3
Or see this region's nucleotide sequence