Strain Fitness in Escherichia coli ECRC100 around OKFHMN_25525

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttccP and espJ are separated by 324 nucleotidesespJ and OKFHMN_25530 are separated by 207 nucleotidesOKFHMN_25530 and OKFHMN_25535 are separated by 50 nucleotides OKFHMN_25520: tccP - Tir-cytoskeleton coupling protein TccP, at 4,940,565 to 4,941,578 tccP OKFHMN_25525: espJ - type III secretion system effector ADP-ribosyltransferase EspJ, at 4,941,903 to 4,942,556 espJ OKFHMN_25530: OKFHMN_25530 - hypothetical protein, at 4,942,764 to 4,942,982 _25530 OKFHMN_25535: OKFHMN_25535 - tRNA-Xxx, at 4,943,033 to 4,943,122 _25535 Position (kb) 4941 4942 4943Strain fitness (log2 ratio) -1 0 1 2at 4941.291 kb on - strand, within tccPat 4941.369 kb on - strand, within tccPat 4941.498 kb on + strandat 4941.522 kb on + strandat 4941.617 kb on + strandat 4941.622 kb on - strandat 4941.634 kb on + strandat 4941.802 kb on + strandat 4941.919 kb on + strandat 4941.922 kb on - strandat 4942.609 kb on - strandat 4942.721 kb on + strandat 4942.809 kb on + strand, within OKFHMN_25530at 4942.834 kb on + strand, within OKFHMN_25530at 4943.031 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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4,941,291 - tccP OKFHMN_25520 0.72 +1.9
4,941,369 - tccP OKFHMN_25520 0.79 -0.8
4,941,498 + +0.1
4,941,522 + +1.4
4,941,617 + +0.2
4,941,622 - +0.3
4,941,634 + -1.5
4,941,802 + -0.5
4,941,919 + +1.7
4,941,922 - -1.0
4,942,609 - -0.2
4,942,721 + +2.0
4,942,809 + OKFHMN_25530 0.21 -0.8
4,942,834 + OKFHMN_25530 0.32 +0.4
4,943,031 + +0.7

Or see this region's nucleotide sequence