Strain Fitness in Escherichia coli ECRC100 around OKFHMN_24000

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_23995 and OKFHMN_24000 are separated by 189 nucleotidesOKFHMN_24000 and metG are separated by 140 nucleotides OKFHMN_23995: OKFHMN_23995 - regulator, at 4,679,770 to 4,682,661 _23995 OKFHMN_24000: OKFHMN_24000 - WGR domain-containing protein, at 4,682,851 to 4,683,579 _24000 OKFHMN_24005: metG - methionine--tRNA ligase, at 4,683,720 to 4,685,753 metG Position (kb) 4682 4683 4684Strain fitness (log2 ratio) -1 0 1 2at 4681.871 kb on - strand, within OKFHMN_23995at 4681.977 kb on - strand, within OKFHMN_23995at 4682.118 kb on + strand, within OKFHMN_23995at 4682.197 kb on - strand, within OKFHMN_23995at 4682.271 kb on + strand, within OKFHMN_23995at 4682.345 kb on - strand, within OKFHMN_23995at 4682.349 kb on - strand, within OKFHMN_23995at 4682.628 kb on + strandat 4682.689 kb on + strandat 4682.690 kb on - strandat 4682.690 kb on - strandat 4682.695 kb on + strandat 4682.695 kb on + strandat 4682.695 kb on + strandat 4682.782 kb on + strandat 4682.839 kb on + strandat 4682.852 kb on + strandat 4682.856 kb on + strandat 4682.856 kb on + strandat 4683.152 kb on + strand, within OKFHMN_24000at 4683.619 kb on + strandat 4683.625 kb on + strandat 4683.722 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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4,681,871 - OKFHMN_23995 0.73 -1.7
4,681,977 - OKFHMN_23995 0.76 -0.8
4,682,118 + OKFHMN_23995 0.81 +2.3
4,682,197 - OKFHMN_23995 0.84 +0.7
4,682,271 + OKFHMN_23995 0.86 -0.1
4,682,345 - OKFHMN_23995 0.89 +0.5
4,682,349 - OKFHMN_23995 0.89 -0.3
4,682,628 + -1.4
4,682,689 + -0.3
4,682,690 - -1.2
4,682,690 - -1.0
4,682,695 + -1.5
4,682,695 + +0.3
4,682,695 + +0.3
4,682,782 + +0.7
4,682,839 + -0.2
4,682,852 + +0.5
4,682,856 + -0.2
4,682,856 + +0.2
4,683,152 + OKFHMN_24000 0.41 +0.4
4,683,619 + +0.4
4,683,625 + -0.3
4,683,722 - -0.5

Or see this region's nucleotide sequence