Strain Fitness in Escherichia coli ECRC100 around OKFHMN_23755

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntIle2_trna and Arg_trna are separated by 9 nucleotidesArg_trna and Arg_trna are separated by 13 nucleotidesArg_trna and OKFHMN_23755 are separated by 511 nucleotidesOKFHMN_23755 and OKFHMN_23760 are separated by 136 nucleotidesOKFHMN_23760 and OKFHMN_23765 are separated by 40 nucleotidesOKFHMN_23765 and OKFHMN_23770 are separated by 77 nucleotidesOKFHMN_23770 and OKFHMN_23775 are separated by 4 nucleotides OKFHMN_23730: Ile2_trna - tRNA-Ile2(cat), at 4,633,873 to 4,633,948 _trna OKFHMN_23735: Arg_trna - tRNA-Arg(tcg), at 4,633,958 to 4,634,034 _trna OKFHMN_23740: Arg_trna - tRNA-Arg(tct), at 4,634,048 to 4,634,124 _trna OKFHMN_23755: OKFHMN_23755 - sialate O-acetylesterase, at 4,634,636 to 4,636,582 _23755 OKFHMN_23760: OKFHMN_23760 - DUF1378 domain-containing protein, at 4,636,719 to 4,636,898 _23760 OKFHMN_23765: OKFHMN_23765 - DUF826 domain-containing protein, at 4,636,939 to 4,637,184 _23765 OKFHMN_23770: OKFHMN_23770 - holin, at 4,637,262 to 4,637,477 _23770 OKFHMN_23775: OKFHMN_23775 - SAR-endolysin, at 4,637,482 to 4,638,015 _23775 Position (kb) 4634 4635 4636 4637Strain fitness (log2 ratio) -1 0 1at 4633.718 kb on + strandat 4633.719 kb on - strandat 4633.813 kb on + strandat 4633.814 kb on - strandat 4633.833 kb on + strandat 4633.952 kb on + strandat 4633.953 kb on - strandat 4633.953 kb on - strandat 4633.962 kb on + strandat 4634.135 kb on - strandat 4634.197 kb on + strandat 4634.197 kb on + strandat 4634.251 kb on + strandat 4634.847 kb on + strand, within OKFHMN_23755at 4634.882 kb on - strand, within OKFHMN_23755

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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4,633,718 + +0.4
4,633,719 - -0.3
4,633,813 + +0.3
4,633,814 - -0.3
4,633,833 + -0.8
4,633,952 + -0.8
4,633,953 - -1.0
4,633,953 - -0.3
4,633,962 + -1.1
4,634,135 - +0.7
4,634,197 + -0.4
4,634,197 + -0.2
4,634,251 + +0.9
4,634,847 + OKFHMN_23755 0.11 -0.3
4,634,882 - OKFHMN_23755 0.13 -0.5

Or see this region's nucleotide sequence