Experiment: JK42
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yohD and yohC are separated by 169 nucleotides yohC and pbpG are separated by 173 nucleotides pbpG and dld are separated by 37 nucleotides
OKFHMN_23635: yohD - Inner membrane protein YohD, at 4,618,065 to 4,618,643
yohD
OKFHMN_23640: yohC - Inner membrane protein YohC, at 4,618,813 to 4,619,400
yohC
OKFHMN_23645: pbpG - D-alanyl-D-alanine endopeptidase, at 4,619,574 to 4,620,506
pbpG
OKFHMN_23650: dld - D-lactate dehydrogenase, at 4,620,544 to 4,622,259
dld
Position (kb)
4619
4620
4621 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4618.609 kb on + strand at 4618.615 kb on + strand at 4618.632 kb on - strand at 4618.673 kb on + strand at 4618.673 kb on + strand at 4618.674 kb on - strand at 4618.679 kb on + strand at 4618.680 kb on - strand at 4618.714 kb on + strand at 4618.715 kb on - strand at 4618.768 kb on + strand at 4618.769 kb on - strand at 4618.770 kb on + strand at 4618.813 kb on - strand at 4618.813 kb on - strand at 4618.866 kb on + strand at 4618.898 kb on - strand, within yohC at 4619.046 kb on + strand, within yohC at 4619.188 kb on + strand, within yohC at 4619.250 kb on - strand, within yohC at 4619.369 kb on + strand at 4619.369 kb on + strand at 4619.370 kb on - strand at 4619.370 kb on - strand at 4619.596 kb on + strand at 4619.597 kb on - strand at 4619.720 kb on + strand, within pbpG at 4619.847 kb on - strand, within pbpG at 4619.992 kb on - strand, within pbpG at 4620.003 kb on - strand, within pbpG at 4620.154 kb on + strand, within pbpG at 4620.198 kb on + strand, within pbpG at 4620.198 kb on + strand, within pbpG at 4620.198 kb on + strand, within pbpG at 4620.221 kb on + strand, within pbpG at 4620.222 kb on - strand, within pbpG at 4620.275 kb on + strand, within pbpG at 4620.340 kb on + strand, within pbpG at 4620.340 kb on + strand, within pbpG at 4620.364 kb on - strand, within pbpG at 4620.396 kb on - strand, within pbpG at 4620.448 kb on - strand at 4620.545 kb on + strand at 4620.600 kb on + strand at 4620.600 kb on + strand at 4620.655 kb on - strand at 4620.657 kb on - strand at 4620.672 kb on + strand at 4620.687 kb on - strand at 4620.764 kb on + strand, within dld at 4620.782 kb on + strand, within dld at 4620.783 kb on - strand, within dld at 4620.803 kb on + strand, within dld at 4620.852 kb on - strand, within dld at 4620.852 kb on - strand, within dld at 4621.098 kb on - strand, within dld at 4621.236 kb on + strand, within dld at 4621.316 kb on + strand, within dld at 4621.332 kb on + strand, within dld at 4621.332 kb on + strand, within dld at 4621.332 kb on + strand, within dld at 4621.332 kb on + strand, within dld at 4621.332 kb on + strand, within dld at 4621.333 kb on - strand, within dld at 4621.334 kb on + strand, within dld at 4621.335 kb on - strand, within dld at 4621.455 kb on + strand, within dld at 4621.456 kb on - strand, within dld
Per-strain Table
Position Strand Gene LocusTag Fraction JK42 remove 4,618,609 + -1.1 4,618,615 + +0.2 4,618,632 - +0.7 4,618,673 + -0.5 4,618,673 + -1.0 4,618,674 - -1.5 4,618,679 + -0.4 4,618,680 - +0.0 4,618,714 + -1.0 4,618,715 - +0.2 4,618,768 + +2.3 4,618,769 - +1.3 4,618,770 + +1.3 4,618,813 - +1.3 4,618,813 - -0.3 4,618,866 + -0.3 4,618,898 - yohC OKFHMN_23640 0.14 +0.3 4,619,046 + yohC OKFHMN_23640 0.40 +0.0 4,619,188 + yohC OKFHMN_23640 0.64 +0.9 4,619,250 - yohC OKFHMN_23640 0.74 -1.1 4,619,369 + -1.1 4,619,369 + +1.3 4,619,370 - +3.3 4,619,370 - +0.2 4,619,596 + -0.7 4,619,597 - +0.3 4,619,720 + pbpG OKFHMN_23645 0.16 +0.5 4,619,847 - pbpG OKFHMN_23645 0.29 +0.3 4,619,992 - pbpG OKFHMN_23645 0.45 -2.3 4,620,003 - pbpG OKFHMN_23645 0.46 +1.7 4,620,154 + pbpG OKFHMN_23645 0.62 -0.2 4,620,198 + pbpG OKFHMN_23645 0.67 +1.3 4,620,198 + pbpG OKFHMN_23645 0.67 -1.9 4,620,198 + pbpG OKFHMN_23645 0.67 -1.4 4,620,221 + pbpG OKFHMN_23645 0.69 +0.5 4,620,222 - pbpG OKFHMN_23645 0.69 +3.3 4,620,275 + pbpG OKFHMN_23645 0.75 +0.5 4,620,340 + pbpG OKFHMN_23645 0.82 -0.5 4,620,340 + pbpG OKFHMN_23645 0.82 -0.1 4,620,364 - pbpG OKFHMN_23645 0.85 +0.2 4,620,396 - pbpG OKFHMN_23645 0.88 +0.3 4,620,448 - +1.1 4,620,545 + -0.0 4,620,600 + -1.4 4,620,600 + -0.2 4,620,655 - +0.1 4,620,657 - -0.3 4,620,672 + +0.9 4,620,687 - -0.7 4,620,764 + dld OKFHMN_23650 0.13 +1.1 4,620,782 + dld OKFHMN_23650 0.14 +0.5 4,620,783 - dld OKFHMN_23650 0.14 -0.6 4,620,803 + dld OKFHMN_23650 0.15 -1.3 4,620,852 - dld OKFHMN_23650 0.18 +0.1 4,620,852 - dld OKFHMN_23650 0.18 +0.4 4,621,098 - dld OKFHMN_23650 0.32 -0.5 4,621,236 + dld OKFHMN_23650 0.40 -0.1 4,621,316 + dld OKFHMN_23650 0.45 +1.3 4,621,332 + dld OKFHMN_23650 0.46 +1.4 4,621,332 + dld OKFHMN_23650 0.46 -0.4 4,621,332 + dld OKFHMN_23650 0.46 +0.5 4,621,332 + dld OKFHMN_23650 0.46 +0.9 4,621,332 + dld OKFHMN_23650 0.46 +0.5 4,621,333 - dld OKFHMN_23650 0.46 -0.9 4,621,334 + dld OKFHMN_23650 0.46 +0.9 4,621,335 - dld OKFHMN_23650 0.46 +0.1 4,621,455 + dld OKFHMN_23650 0.53 -0.3 4,621,456 - dld OKFHMN_23650 0.53 +0.0
Or see this region's nucleotide sequence