Strain Fitness in Escherichia coli ECRC100 around OKFHMN_23130

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntubiG and gyrA are separated by 146 nucleotidesgyrA and yfaA are separated by 282 nucleotides OKFHMN_23125: ubiG - bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG, at 4,507,298 to 4,508,020 ubiG OKFHMN_23130: gyrA - DNA topoisomerase (ATP-hydrolyzing) subunit A, at 4,508,167 to 4,510,794 gyrA OKFHMN_23135: yfaA - DUF2138 domain-containing protein, at 4,511,077 to 4,512,630 yfaA Position (kb) 4508 4509 4510 4511Strain fitness (log2 ratio) -1 0 1at 4507.176 kb on + strandat 4507.211 kb on + strandat 4507.211 kb on + strandat 4507.227 kb on - strandat 4508.038 kb on - strandat 4508.059 kb on + strandat 4508.059 kb on + strandat 4508.060 kb on - strandat 4510.926 kb on - strandat 4511.038 kb on + strandat 4511.480 kb on + strand, within yfaAat 4511.549 kb on - strand, within yfaAat 4511.592 kb on + strand, within yfaAat 4511.784 kb on - strand, within yfaA

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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4,507,176 + -0.7
4,507,211 + +0.6
4,507,211 + -0.9
4,507,227 - +0.2
4,508,038 - +0.0
4,508,059 + +0.8
4,508,059 + +0.3
4,508,060 - +0.1
4,510,926 - +0.3
4,511,038 + -1.5
4,511,480 + yfaA OKFHMN_23135 0.26 +0.8
4,511,549 - yfaA OKFHMN_23135 0.30 +0.3
4,511,592 + yfaA OKFHMN_23135 0.33 -0.8
4,511,784 - yfaA OKFHMN_23135 0.45 +0.2

Or see this region's nucleotide sequence