Strain Fitness in Escherichia coli ECRC100 around OKFHMN_22375

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntalaC and ypdK are separated by 64 nucleotidesypdK and lpxP are separated by 370 nucleotides OKFHMN_22370: alaC - alanine transaminase, at 4,348,152 to 4,349,390 alaC OKFHMN_22375: ypdK - membrane protein YpdK, at 4,349,455 to 4,349,511 ypdK OKFHMN_22380: lpxP - kdo(2)-lipid IV(A) palmitoleoyltransferase, at 4,349,882 to 4,350,802 lpxP Position (kb) 4349 4350Strain fitness (log2 ratio) -1 0 1at 4348.557 kb on - strand, within alaCat 4348.561 kb on - strand, within alaCat 4348.758 kb on + strand, within alaCat 4348.788 kb on + strand, within alaCat 4348.789 kb on - strand, within alaCat 4348.803 kb on + strand, within alaCat 4349.058 kb on + strand, within alaCat 4349.078 kb on - strand, within alaCat 4349.163 kb on + strand, within alaCat 4349.298 kb on - strandat 4349.389 kb on - strandat 4349.497 kb on - strand, within ypdKat 4349.548 kb on - strandat 4349.582 kb on - strandat 4349.582 kb on - strandat 4349.582 kb on - strandat 4349.624 kb on - strandat 4349.992 kb on - strand, within lpxPat 4350.087 kb on - strand, within lpxPat 4350.126 kb on + strand, within lpxPat 4350.127 kb on - strand, within lpxPat 4350.286 kb on + strand, within lpxPat 4350.488 kb on - strand, within lpxPat 4350.492 kb on - strand, within lpxP

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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4,348,557 - alaC OKFHMN_22370 0.33 +0.4
4,348,561 - alaC OKFHMN_22370 0.33 +0.4
4,348,758 + alaC OKFHMN_22370 0.49 +0.1
4,348,788 + alaC OKFHMN_22370 0.51 +1.1
4,348,789 - alaC OKFHMN_22370 0.51 +0.6
4,348,803 + alaC OKFHMN_22370 0.53 +1.0
4,349,058 + alaC OKFHMN_22370 0.73 +1.4
4,349,078 - alaC OKFHMN_22370 0.75 +0.8
4,349,163 + alaC OKFHMN_22370 0.82 -0.1
4,349,298 - -0.0
4,349,389 - -0.2
4,349,497 - ypdK OKFHMN_22375 0.74 +0.3
4,349,548 - +0.9
4,349,582 - +0.0
4,349,582 - +0.0
4,349,582 - -0.2
4,349,624 - -0.5
4,349,992 - lpxP OKFHMN_22380 0.12 +0.0
4,350,087 - lpxP OKFHMN_22380 0.22 -0.0
4,350,126 + lpxP OKFHMN_22380 0.26 -0.4
4,350,127 - lpxP OKFHMN_22380 0.27 +0.0
4,350,286 + lpxP OKFHMN_22380 0.44 -0.0
4,350,488 - lpxP OKFHMN_22380 0.66 -0.2
4,350,492 - lpxP OKFHMN_22380 0.66 +1.0

Or see this region's nucleotide sequence