Strain Fitness in Escherichia coli ECRC100 around OKFHMN_20365

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygcN and queD are separated by 77 nucleotidesqueD and cysJ are separated by 315 nucleotides OKFHMN_20360: ygcN - putative electron transfer flavoprotein-quinone oxidoreductase YgcN, at 3,962,343 to 3,963,614 ygcN OKFHMN_20365: queD - 6-carboxytetrahydropterin synthase QueD, at 3,963,692 to 3,964,057 queD OKFHMN_20370: cysJ - NADPH-dependent assimilatory sulfite reductase flavoprotein subunit, at 3,964,373 to 3,966,172 cysJ Position (kb) 3963 3964 3965Strain fitness (log2 ratio) -1 0 1 2at 3962.859 kb on - strand, within ygcNat 3962.948 kb on + strand, within ygcNat 3962.949 kb on - strand, within ygcNat 3962.949 kb on - strand, within ygcNat 3963.048 kb on - strand, within ygcNat 3963.118 kb on - strand, within ygcNat 3963.118 kb on - strand, within ygcNat 3963.319 kb on - strand, within ygcNat 3963.464 kb on + strand, within ygcNat 3963.466 kb on + strand, within ygcNat 3963.467 kb on - strand, within ygcNat 3963.469 kb on - strand, within ygcNat 3963.469 kb on - strand, within ygcNat 3963.590 kb on + strandat 3963.590 kb on + strandat 3963.591 kb on - strandat 3963.591 kb on - strandat 3963.593 kb on + strandat 3963.593 kb on + strandat 3963.594 kb on - strandat 3963.647 kb on + strandat 3963.652 kb on + strandat 3963.653 kb on - strandat 3963.659 kb on - strandat 3963.659 kb on - strandat 3963.690 kb on + strandat 3963.710 kb on - strandat 3963.805 kb on + strand, within queDat 3963.829 kb on + strand, within queDat 3963.830 kb on - strand, within queDat 3963.861 kb on + strand, within queDat 3963.861 kb on + strand, within queDat 3963.862 kb on - strand, within queDat 3963.862 kb on - strand, within queDat 3963.885 kb on - strand, within queDat 3963.885 kb on - strand, within queDat 3963.885 kb on - strand, within queDat 3963.983 kb on - strand, within queDat 3964.041 kb on - strandat 3964.076 kb on + strandat 3964.118 kb on - strandat 3964.251 kb on - strandat 3964.254 kb on - strandat 3964.365 kb on - strandat 3964.563 kb on + strand, within cysJat 3964.564 kb on - strand, within cysJat 3964.564 kb on - strand, within cysJat 3964.564 kb on - strand, within cysJat 3964.567 kb on + strand, within cysJat 3964.568 kb on - strand, within cysJat 3964.568 kb on - strand, within cysJat 3964.570 kb on + strand, within cysJat 3964.571 kb on - strand, within cysJat 3964.769 kb on - strand, within cysJat 3964.807 kb on + strand, within cysJ

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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3,962,859 - ygcN OKFHMN_20360 0.41 +0.5
3,962,948 + ygcN OKFHMN_20360 0.48 -1.7
3,962,949 - ygcN OKFHMN_20360 0.48 +0.4
3,962,949 - ygcN OKFHMN_20360 0.48 -0.3
3,963,048 - ygcN OKFHMN_20360 0.55 -0.2
3,963,118 - ygcN OKFHMN_20360 0.61 +1.2
3,963,118 - ygcN OKFHMN_20360 0.61 +1.1
3,963,319 - ygcN OKFHMN_20360 0.77 -0.8
3,963,464 + ygcN OKFHMN_20360 0.88 -1.3
3,963,466 + ygcN OKFHMN_20360 0.88 +0.3
3,963,467 - ygcN OKFHMN_20360 0.88 +0.1
3,963,469 - ygcN OKFHMN_20360 0.89 -0.3
3,963,469 - ygcN OKFHMN_20360 0.89 +0.5
3,963,590 + +0.9
3,963,590 + +0.2
3,963,591 - +0.8
3,963,591 - -0.5
3,963,593 + -0.1
3,963,593 + +0.2
3,963,594 - -0.3
3,963,647 + +0.9
3,963,652 + -0.1
3,963,653 - +0.2
3,963,659 - -0.4
3,963,659 - +0.4
3,963,690 + -0.6
3,963,710 - +0.6
3,963,805 + queD OKFHMN_20365 0.31 +1.0
3,963,829 + queD OKFHMN_20365 0.37 -0.6
3,963,830 - queD OKFHMN_20365 0.38 +0.4
3,963,861 + queD OKFHMN_20365 0.46 -0.1
3,963,861 + queD OKFHMN_20365 0.46 +0.2
3,963,862 - queD OKFHMN_20365 0.46 +1.1
3,963,862 - queD OKFHMN_20365 0.46 -0.1
3,963,885 - queD OKFHMN_20365 0.53 +0.6
3,963,885 - queD OKFHMN_20365 0.53 -1.6
3,963,885 - queD OKFHMN_20365 0.53 +0.5
3,963,983 - queD OKFHMN_20365 0.80 +0.7
3,964,041 - +0.1
3,964,076 + +2.5
3,964,118 - -0.3
3,964,251 - -0.1
3,964,254 - -1.1
3,964,365 - -0.0
3,964,563 + cysJ OKFHMN_20370 0.11 +0.4
3,964,564 - cysJ OKFHMN_20370 0.11 +0.1
3,964,564 - cysJ OKFHMN_20370 0.11 -0.3
3,964,564 - cysJ OKFHMN_20370 0.11 +0.5
3,964,567 + cysJ OKFHMN_20370 0.11 -0.0
3,964,568 - cysJ OKFHMN_20370 0.11 -0.1
3,964,568 - cysJ OKFHMN_20370 0.11 +0.6
3,964,570 + cysJ OKFHMN_20370 0.11 -0.6
3,964,571 - cysJ OKFHMN_20370 0.11 -1.2
3,964,769 - cysJ OKFHMN_20370 0.22 +0.5
3,964,807 + cysJ OKFHMN_20370 0.24 +0.1

Or see this region's nucleotide sequence