Strain Fitness in Escherichia coli ECRC100 around OKFHMN_20140

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrlmM and fucR are separated by 43 nucleotidesfucR and fucU are separated by 57 nucleotidesfucU and fucK are separated by 1 nucleotides OKFHMN_20130: rlmM - 23S rRNA (cytidine(2498)-2'-O)-methyltransferase RlmM, at 3,912,747 to 3,913,847 rlmM OKFHMN_20135: fucR - L-fucose operon activator, at 3,913,891 to 3,914,622 fucR OKFHMN_20140: fucU - L-fucose mutarotase, at 3,914,680 to 3,915,102 fucU OKFHMN_20145: fucK - L-fuculokinase, at 3,915,104 to 3,916,522 fucK Position (kb) 3914 3915 3916Strain fitness (log2 ratio) -1 0 1at 3913.710 kb on + strand, within rlmMat 3913.711 kb on - strand, within rlmMat 3913.741 kb on + strandat 3913.742 kb on - strandat 3913.770 kb on + strandat 3913.771 kb on - strandat 3913.808 kb on + strandat 3913.845 kb on + strandat 3913.880 kb on + strandat 3913.946 kb on - strandat 3913.999 kb on - strand, within fucRat 3914.009 kb on - strand, within fucRat 3914.036 kb on - strand, within fucRat 3914.036 kb on - strand, within fucRat 3914.062 kb on - strand, within fucRat 3914.168 kb on + strand, within fucRat 3914.168 kb on + strand, within fucRat 3914.303 kb on + strand, within fucRat 3914.394 kb on - strand, within fucRat 3914.540 kb on - strand, within fucRat 3914.681 kb on + strandat 3914.710 kb on - strandat 3914.710 kb on - strandat 3914.717 kb on + strandat 3914.740 kb on - strand, within fucUat 3914.771 kb on + strand, within fucUat 3914.771 kb on + strand, within fucUat 3914.819 kb on - strand, within fucUat 3914.819 kb on - strand, within fucUat 3914.832 kb on + strand, within fucUat 3914.876 kb on - strand, within fucUat 3914.906 kb on + strand, within fucUat 3914.906 kb on + strand, within fucUat 3915.283 kb on + strand, within fucKat 3915.353 kb on - strand, within fucKat 3915.488 kb on + strand, within fucKat 3915.625 kb on + strand, within fucKat 3915.626 kb on - strand, within fucKat 3915.636 kb on + strand, within fucKat 3915.637 kb on - strand, within fucKat 3915.637 kb on - strand, within fucKat 3915.686 kb on + strand, within fucKat 3915.698 kb on - strand, within fucKat 3915.997 kb on + strand, within fucK

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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3,913,710 + rlmM OKFHMN_20130 0.87 +0.2
3,913,711 - rlmM OKFHMN_20130 0.88 -1.3
3,913,741 + -0.5
3,913,742 - +1.5
3,913,770 + +1.2
3,913,771 - -0.5
3,913,808 + -0.4
3,913,845 + +0.3
3,913,880 + +0.2
3,913,946 - -0.3
3,913,999 - fucR OKFHMN_20135 0.15 +0.2
3,914,009 - fucR OKFHMN_20135 0.16 -0.0
3,914,036 - fucR OKFHMN_20135 0.20 -0.5
3,914,036 - fucR OKFHMN_20135 0.20 -0.4
3,914,062 - fucR OKFHMN_20135 0.23 -0.0
3,914,168 + fucR OKFHMN_20135 0.38 +0.3
3,914,168 + fucR OKFHMN_20135 0.38 +0.1
3,914,303 + fucR OKFHMN_20135 0.56 +0.9
3,914,394 - fucR OKFHMN_20135 0.69 +1.5
3,914,540 - fucR OKFHMN_20135 0.89 -0.5
3,914,681 + +0.8
3,914,710 - +1.2
3,914,710 - -0.2
3,914,717 + -0.2
3,914,740 - fucU OKFHMN_20140 0.14 -0.9
3,914,771 + fucU OKFHMN_20140 0.22 +0.4
3,914,771 + fucU OKFHMN_20140 0.22 +0.7
3,914,819 - fucU OKFHMN_20140 0.33 -1.1
3,914,819 - fucU OKFHMN_20140 0.33 -0.3
3,914,832 + fucU OKFHMN_20140 0.36 -0.9
3,914,876 - fucU OKFHMN_20140 0.46 +0.9
3,914,906 + fucU OKFHMN_20140 0.53 -0.6
3,914,906 + fucU OKFHMN_20140 0.53 -0.5
3,915,283 + fucK OKFHMN_20145 0.13 +0.2
3,915,353 - fucK OKFHMN_20145 0.18 -0.2
3,915,488 + fucK OKFHMN_20145 0.27 +0.8
3,915,625 + fucK OKFHMN_20145 0.37 +0.1
3,915,626 - fucK OKFHMN_20145 0.37 +0.7
3,915,636 + fucK OKFHMN_20145 0.37 -0.5
3,915,637 - fucK OKFHMN_20145 0.38 -0.9
3,915,637 - fucK OKFHMN_20145 0.38 +0.3
3,915,686 + fucK OKFHMN_20145 0.41 +0.2
3,915,698 - fucK OKFHMN_20145 0.42 +0.3
3,915,997 + fucK OKFHMN_20145 0.63 -0.8

Or see this region's nucleotide sequence