Strain Fitness in Escherichia coli ECRC100 around OKFHMN_17495

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrpoA and OKFHMN_17490 are separated by 40 nucleotidesOKFHMN_17490 and yhdN are separated by 106 nucleotidesyhdN and zntR are separated by 10 nucleotideszntR and arfA are separated by 55 nucleotidesarfA and mscL overlap by 4 nucleotides OKFHMN_17485: rpoA - DNA-directed RNA polymerase subunit alpha, at 3,399,040 to 3,400,029 rpoA OKFHMN_17490: OKFHMN_17490 - 50S ribosomal protein L17, at 3,400,070 to 3,400,453 _17490 OKFHMN_17495: yhdN - Uncharacterized protein YhdN, at 3,400,560 to 3,400,928 yhdN OKFHMN_17500: zntR - Zn(2+)-responsive transcriptional regulator, at 3,400,939 to 3,401,364 zntR OKFHMN_17505: arfA - alternative ribosome-rescue factor ArfA, at 3,401,420 to 3,401,638 arfA OKFHMN_17510: mscL - large-conductance mechanosensitive channel protein MscL, at 3,401,635 to 3,402,045 mscL Position (kb) 3400 3401Strain fitness (log2 ratio) -1 0 1 2at 3400.515 kb on + strandat 3400.515 kb on + strandat 3400.515 kb on + strandat 3400.516 kb on - strandat 3400.555 kb on + strandat 3400.555 kb on + strandat 3400.556 kb on - strandat 3400.556 kb on - strandat 3400.567 kb on + strandat 3400.567 kb on + strandat 3400.592 kb on + strandat 3400.595 kb on - strandat 3400.926 kb on + strandat 3400.926 kb on + strandat 3400.934 kb on + strandat 3400.934 kb on + strandat 3400.935 kb on - strandat 3400.935 kb on - strandat 3400.938 kb on + strandat 3400.938 kb on + strandat 3400.938 kb on + strandat 3400.938 kb on + strandat 3400.938 kb on + strandat 3400.939 kb on - strandat 3400.939 kb on - strandat 3400.939 kb on - strandat 3400.942 kb on + strandat 3401.108 kb on + strand, within zntRat 3401.169 kb on + strand, within zntRat 3401.170 kb on - strand, within zntRat 3401.170 kb on - strand, within zntRat 3401.170 kb on - strand, within zntRat 3401.170 kb on - strand, within zntRat 3401.170 kb on - strand, within zntRat 3401.170 kb on - strand, within zntRat 3401.295 kb on + strand, within zntRat 3401.295 kb on + strand, within zntRat 3401.384 kb on + strandat 3401.385 kb on - strandat 3401.438 kb on - strandat 3401.438 kb on - strandat 3401.440 kb on + strandat 3401.440 kb on + strandat 3401.466 kb on + strand, within arfAat 3401.475 kb on + strand, within arfAat 3401.476 kb on - strand, within arfAat 3401.506 kb on - strand, within arfAat 3401.636 kb on + strandat 3401.636 kb on + strandat 3401.686 kb on + strand, within mscLat 3401.687 kb on - strand, within mscLat 3401.687 kb on - strand, within mscLat 3401.687 kb on - strand, within mscLat 3401.752 kb on + strand, within mscLat 3401.753 kb on - strand, within mscLat 3401.906 kb on - strand, within mscL

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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3,400,515 + -0.4
3,400,515 + +1.1
3,400,515 + -0.9
3,400,516 - -0.7
3,400,555 + +0.3
3,400,555 + -0.1
3,400,556 - +0.5
3,400,556 - -0.6
3,400,567 + +0.3
3,400,567 + -0.2
3,400,592 + +0.2
3,400,595 - +0.5
3,400,926 + +0.2
3,400,926 + -0.5
3,400,934 + +0.5
3,400,934 + +0.3
3,400,935 - +0.4
3,400,935 - +0.7
3,400,938 + -0.6
3,400,938 + +0.2
3,400,938 + -0.7
3,400,938 + -0.8
3,400,938 + +0.4
3,400,939 - -0.9
3,400,939 - +1.1
3,400,939 - -0.3
3,400,942 + -0.1
3,401,108 + zntR OKFHMN_17500 0.40 +1.0
3,401,169 + zntR OKFHMN_17500 0.54 -0.7
3,401,170 - zntR OKFHMN_17500 0.54 -0.4
3,401,170 - zntR OKFHMN_17500 0.54 +0.5
3,401,170 - zntR OKFHMN_17500 0.54 -0.1
3,401,170 - zntR OKFHMN_17500 0.54 -0.5
3,401,170 - zntR OKFHMN_17500 0.54 -0.3
3,401,170 - zntR OKFHMN_17500 0.54 +0.1
3,401,295 + zntR OKFHMN_17500 0.84 -0.8
3,401,295 + zntR OKFHMN_17500 0.84 -0.9
3,401,384 + +2.1
3,401,385 - -0.3
3,401,438 - -0.3
3,401,438 - -0.8
3,401,440 + +1.1
3,401,440 + -1.2
3,401,466 + arfA OKFHMN_17505 0.21 +0.7
3,401,475 + arfA OKFHMN_17505 0.25 -0.2
3,401,476 - arfA OKFHMN_17505 0.26 -0.2
3,401,506 - arfA OKFHMN_17505 0.39 +1.2
3,401,636 + -1.5
3,401,636 + -1.2
3,401,686 + mscL OKFHMN_17510 0.12 +0.0
3,401,687 - mscL OKFHMN_17510 0.13 +1.3
3,401,687 - mscL OKFHMN_17510 0.13 +0.1
3,401,687 - mscL OKFHMN_17510 0.13 -1.0
3,401,752 + mscL OKFHMN_17510 0.28 -0.6
3,401,753 - mscL OKFHMN_17510 0.29 -0.5
3,401,906 - mscL OKFHMN_17510 0.66 -0.6

Or see this region's nucleotide sequence