Strain Fitness in Escherichia coli ECRC100 around OKFHMN_17360

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpulO and rpsJ are separated by 378 nucleotidesrpsJ and rplC are separated by 32 nucleotidesrplC and rplD are separated by 10 nucleotidesrplD and rplW overlap by 4 nucleotidesrplW and rplB are separated by 17 nucleotides OKFHMN_17350: pulO - prepilin peptidase, at 3,385,953 to 3,386,420 pulO OKFHMN_17355: rpsJ - 30S ribosomal protein S10, at 3,386,799 to 3,387,110 rpsJ OKFHMN_17360: rplC - 50S ribosomal protein L3, at 3,387,143 to 3,387,772 rplC OKFHMN_17365: rplD - 50S ribosomal protein L4, at 3,387,783 to 3,388,388 rplD OKFHMN_17370: rplW - 50S ribosomal protein L23, at 3,388,385 to 3,388,687 rplW OKFHMN_17375: rplB - 50S ribosomal protein L2, at 3,388,705 to 3,389,526 rplB Position (kb) 3387 3388Strain fitness (log2 ratio) -2 -1 0 1at 3386.171 kb on - strand, within pulOat 3386.171 kb on - strand, within pulOat 3386.175 kb on + strand, within pulOat 3386.175 kb on + strand, within pulOat 3386.176 kb on - strand, within pulOat 3386.176 kb on - strand, within pulOat 3386.241 kb on - strand, within pulOat 3386.253 kb on + strand, within pulOat 3386.324 kb on + strand, within pulOat 3386.324 kb on + strand, within pulOat 3386.325 kb on - strand, within pulOat 3386.337 kb on + strand, within pulOat 3386.412 kb on - strandat 3386.412 kb on - strandat 3386.412 kb on - strandat 3386.434 kb on + strandat 3386.435 kb on - strandat 3386.457 kb on - strandat 3386.686 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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3,386,171 - pulO OKFHMN_17350 0.47 -0.3
3,386,171 - pulO OKFHMN_17350 0.47 +0.2
3,386,175 + pulO OKFHMN_17350 0.47 +0.2
3,386,175 + pulO OKFHMN_17350 0.47 +0.1
3,386,176 - pulO OKFHMN_17350 0.48 -0.3
3,386,176 - pulO OKFHMN_17350 0.48 +0.7
3,386,241 - pulO OKFHMN_17350 0.62 -0.3
3,386,253 + pulO OKFHMN_17350 0.64 +0.7
3,386,324 + pulO OKFHMN_17350 0.79 -1.9
3,386,324 + pulO OKFHMN_17350 0.79 +0.8
3,386,325 - pulO OKFHMN_17350 0.79 -0.2
3,386,337 + pulO OKFHMN_17350 0.82 -0.3
3,386,412 - +0.3
3,386,412 - +0.0
3,386,412 - -1.7
3,386,434 + -0.0
3,386,435 - +0.1
3,386,457 - -0.1
3,386,686 + +0.7

Or see this region's nucleotide sequence