Experiment: JK42
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt amiB and tsaE are separated by 18 nucleotides tsaE and nnr overlap by 29 nucleotides nnr and queG are separated by 13 nucleotides
OKFHMN_12270: amiB - N-acetylmuramoyl-L-alanine amidase AmiB, at 2,350,399 to 2,351,736
amiB
OKFHMN_12275: tsaE - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE, at 2,351,755 to 2,352,216
tsaE
OKFHMN_12280: nnr - bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase, at 2,352,188 to 2,353,720
nnr
OKFHMN_12285: queG - tRNA epoxyqueuosine(34) reductase QueG, at 2,353,734 to 2,354,873
queG
Position (kb)
2352
2353
2354 Strain fitness (log2 ratio)
-2
-1
0
1 at 2351.211 kb on + strand, within amiB at 2351.212 kb on - strand, within amiB at 2351.212 kb on - strand, within amiB at 2351.299 kb on - strand, within amiB at 2351.381 kb on + strand, within amiB at 2351.381 kb on + strand, within amiB at 2351.382 kb on - strand, within amiB at 2351.382 kb on - strand, within amiB at 2351.467 kb on - strand, within amiB at 2351.500 kb on - strand, within amiB at 2351.500 kb on - strand, within amiB at 2351.607 kb on + strand at 2351.643 kb on + strand at 2351.729 kb on + strand at 2351.729 kb on + strand at 2351.729 kb on + strand at 2351.729 kb on + strand at 2351.729 kb on + strand at 2351.730 kb on - strand at 2352.242 kb on - strand at 2352.248 kb on - strand at 2352.395 kb on - strand, within nnr at 2352.395 kb on - strand, within nnr at 2352.531 kb on - strand, within nnr at 2352.531 kb on - strand, within nnr at 2352.531 kb on - strand, within nnr at 2352.551 kb on + strand, within nnr at 2352.587 kb on + strand, within nnr at 2352.588 kb on - strand, within nnr at 2352.794 kb on - strand, within nnr at 2352.806 kb on - strand, within nnr at 2352.876 kb on - strand, within nnr at 2352.922 kb on + strand, within nnr at 2352.922 kb on + strand, within nnr at 2353.112 kb on - strand, within nnr at 2353.264 kb on + strand, within nnr at 2353.264 kb on + strand, within nnr at 2353.265 kb on - strand, within nnr at 2353.367 kb on - strand, within nnr at 2353.523 kb on - strand, within nnr at 2353.697 kb on + strand at 2353.699 kb on + strand at 2353.700 kb on - strand at 2353.700 kb on - strand at 2353.703 kb on + strand at 2353.704 kb on - strand at 2353.723 kb on + strand at 2353.723 kb on + strand at 2353.724 kb on - strand at 2353.724 kb on - strand at 2353.810 kb on + strand at 2353.814 kb on + strand at 2353.814 kb on + strand at 2353.815 kb on - strand at 2353.815 kb on - strand at 2353.815 kb on - strand at 2353.875 kb on + strand, within queG at 2353.876 kb on - strand, within queG at 2353.876 kb on - strand, within queG at 2353.910 kb on + strand, within queG at 2353.937 kb on - strand, within queG at 2353.977 kb on + strand, within queG at 2353.977 kb on + strand, within queG at 2353.978 kb on - strand, within queG at 2354.032 kb on - strand, within queG at 2354.136 kb on - strand, within queG at 2354.160 kb on - strand, within queG at 2354.207 kb on + strand, within queG at 2354.207 kb on + strand, within queG at 2354.208 kb on - strand, within queG at 2354.208 kb on - strand, within queG at 2354.219 kb on + strand, within queG at 2354.219 kb on + strand, within queG at 2354.220 kb on - strand, within queG at 2354.331 kb on - strand, within queG at 2354.367 kb on + strand, within queG at 2354.368 kb on - strand, within queG at 2354.369 kb on + strand, within queG at 2354.369 kb on + strand, within queG at 2354.369 kb on + strand, within queG at 2354.369 kb on + strand, within queG at 2354.370 kb on - strand, within queG at 2354.390 kb on + strand, within queG at 2354.390 kb on + strand, within queG at 2354.391 kb on - strand, within queG at 2354.398 kb on - strand, within queG at 2354.459 kb on + strand, within queG at 2354.463 kb on + strand, within queG at 2354.552 kb on - strand, within queG at 2354.575 kb on + strand, within queG at 2354.634 kb on - strand, within queG at 2354.660 kb on + strand, within queG at 2354.674 kb on + strand, within queG at 2354.674 kb on + strand, within queG
Per-strain Table
Position Strand Gene LocusTag Fraction JK42 remove 2,351,211 + amiB OKFHMN_12270 0.61 +0.4 2,351,212 - amiB OKFHMN_12270 0.61 +0.2 2,351,212 - amiB OKFHMN_12270 0.61 +0.2 2,351,299 - amiB OKFHMN_12270 0.67 +0.2 2,351,381 + amiB OKFHMN_12270 0.73 +0.2 2,351,381 + amiB OKFHMN_12270 0.73 +0.1 2,351,382 - amiB OKFHMN_12270 0.73 +0.0 2,351,382 - amiB OKFHMN_12270 0.73 -0.5 2,351,467 - amiB OKFHMN_12270 0.80 -0.3 2,351,500 - amiB OKFHMN_12270 0.82 +1.7 2,351,500 - amiB OKFHMN_12270 0.82 -0.1 2,351,607 + +0.5 2,351,643 + +0.5 2,351,729 + +0.2 2,351,729 + -0.9 2,351,729 + +0.2 2,351,729 + -0.4 2,351,729 + -1.1 2,351,730 - +0.0 2,352,242 - -0.9 2,352,248 - +0.5 2,352,395 - nnr OKFHMN_12280 0.14 -0.2 2,352,395 - nnr OKFHMN_12280 0.14 -1.0 2,352,531 - nnr OKFHMN_12280 0.22 +0.2 2,352,531 - nnr OKFHMN_12280 0.22 +0.2 2,352,531 - nnr OKFHMN_12280 0.22 +1.7 2,352,551 + nnr OKFHMN_12280 0.24 +0.1 2,352,587 + nnr OKFHMN_12280 0.26 +0.4 2,352,588 - nnr OKFHMN_12280 0.26 -0.3 2,352,794 - nnr OKFHMN_12280 0.40 +0.2 2,352,806 - nnr OKFHMN_12280 0.40 -0.2 2,352,876 - nnr OKFHMN_12280 0.45 -1.9 2,352,922 + nnr OKFHMN_12280 0.48 -0.7 2,352,922 + nnr OKFHMN_12280 0.48 +1.7 2,353,112 - nnr OKFHMN_12280 0.60 +0.1 2,353,264 + nnr OKFHMN_12280 0.70 -0.5 2,353,264 + nnr OKFHMN_12280 0.70 +0.2 2,353,265 - nnr OKFHMN_12280 0.70 +0.7 2,353,367 - nnr OKFHMN_12280 0.77 -1.1 2,353,523 - nnr OKFHMN_12280 0.87 +1.1 2,353,697 + +1.1 2,353,699 + -0.4 2,353,700 - -0.8 2,353,700 - -1.9 2,353,703 + -0.7 2,353,704 - +0.3 2,353,723 + -0.3 2,353,723 + -0.3 2,353,724 - +0.4 2,353,724 - +0.7 2,353,810 + -1.7 2,353,814 + +0.5 2,353,814 + -1.2 2,353,815 - +1.4 2,353,815 - -0.9 2,353,815 - -2.1 2,353,875 + queG OKFHMN_12285 0.12 +0.4 2,353,876 - queG OKFHMN_12285 0.12 -1.0 2,353,876 - queG OKFHMN_12285 0.12 -1.3 2,353,910 + queG OKFHMN_12285 0.15 -0.9 2,353,937 - queG OKFHMN_12285 0.18 -0.5 2,353,977 + queG OKFHMN_12285 0.21 -0.4 2,353,977 + queG OKFHMN_12285 0.21 -1.4 2,353,978 - queG OKFHMN_12285 0.21 -1.1 2,354,032 - queG OKFHMN_12285 0.26 -1.7 2,354,136 - queG OKFHMN_12285 0.35 -1.2 2,354,160 - queG OKFHMN_12285 0.37 -1.7 2,354,207 + queG OKFHMN_12285 0.41 +0.0 2,354,207 + queG OKFHMN_12285 0.41 +1.7 2,354,208 - queG OKFHMN_12285 0.42 -0.3 2,354,208 - queG OKFHMN_12285 0.42 -1.4 2,354,219 + queG OKFHMN_12285 0.43 -1.5 2,354,219 + queG OKFHMN_12285 0.43 -0.7 2,354,220 - queG OKFHMN_12285 0.43 +0.2 2,354,331 - queG OKFHMN_12285 0.52 +0.8 2,354,367 + queG OKFHMN_12285 0.56 +0.7 2,354,368 - queG OKFHMN_12285 0.56 -0.3 2,354,369 + queG OKFHMN_12285 0.56 -0.6 2,354,369 + queG OKFHMN_12285 0.56 -1.9 2,354,369 + queG OKFHMN_12285 0.56 +0.0 2,354,369 + queG OKFHMN_12285 0.56 -1.1 2,354,370 - queG OKFHMN_12285 0.56 -0.2 2,354,390 + queG OKFHMN_12285 0.58 -1.4 2,354,390 + queG OKFHMN_12285 0.58 -0.9 2,354,391 - queG OKFHMN_12285 0.58 +0.0 2,354,398 - queG OKFHMN_12285 0.58 +0.7 2,354,459 + queG OKFHMN_12285 0.64 -1.0 2,354,463 + queG OKFHMN_12285 0.64 -1.0 2,354,552 - queG OKFHMN_12285 0.72 -1.2 2,354,575 + queG OKFHMN_12285 0.74 -1.2 2,354,634 - queG OKFHMN_12285 0.79 +0.6 2,354,660 + queG OKFHMN_12285 0.81 +0.7 2,354,674 + queG OKFHMN_12285 0.82 +0.2 2,354,674 + queG OKFHMN_12285 0.82 -1.0
Or see this region's nucleotide sequence