Strain Fitness in Escherichia coli ECRC100 around OKFHMN_10800

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpolB and rapA are separated by 163 nucleotidesrapA and rluA are separated by 11 nucleotides OKFHMN_10795: polB - DNA polymerase II, at 2,032,480 to 2,034,831 polB OKFHMN_10800: rapA - RNA polymerase-associated protein RapA, at 2,034,995 to 2,037,901 rapA OKFHMN_10805: rluA - bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA, at 2,037,913 to 2,038,572 rluA Position (kb) 2034 2035 2036 2037 2038Strain fitness (log2 ratio) -1 0 1 2 3 4at 2034.106 kb on + strand, within polBat 2034.260 kb on - strand, within polBat 2034.373 kb on - strand, within polBat 2034.437 kb on - strand, within polBat 2034.444 kb on + strand, within polBat 2034.445 kb on - strand, within polBat 2034.473 kb on - strand, within polBat 2034.529 kb on + strand, within polBat 2034.587 kb on + strand, within polBat 2034.664 kb on - strandat 2034.667 kb on + strandat 2034.668 kb on - strandat 2034.668 kb on - strandat 2034.671 kb on + strandat 2034.671 kb on + strandat 2034.671 kb on + strandat 2034.725 kb on - strandat 2034.733 kb on + strandat 2034.749 kb on + strandat 2034.749 kb on + strandat 2034.771 kb on - strandat 2034.804 kb on + strandat 2034.804 kb on + strandat 2034.804 kb on + strandat 2034.804 kb on + strandat 2034.805 kb on - strandat 2034.805 kb on - strandat 2034.825 kb on - strandat 2034.939 kb on + strandat 2034.939 kb on + strandat 2034.939 kb on + strandat 2034.940 kb on - strandat 2034.972 kb on + strandat 2034.973 kb on - strandat 2034.978 kb on + strandat 2034.979 kb on - strandat 2034.980 kb on + strandat 2034.981 kb on - strandat 2034.994 kb on + strandat 2034.995 kb on - strandat 2034.995 kb on - strandat 2034.995 kb on - strandat 2034.995 kb on - strandat 2034.995 kb on - strandat 2035.003 kb on + strandat 2035.004 kb on - strandat 2035.102 kb on + strandat 2035.102 kb on + strandat 2035.122 kb on - strandat 2035.172 kb on - strandat 2035.177 kb on + strandat 2035.241 kb on + strandat 2035.242 kb on - strandat 2035.361 kb on - strand, within rapAat 2035.393 kb on + strand, within rapAat 2035.393 kb on + strand, within rapAat 2035.394 kb on - strand, within rapAat 2035.447 kb on + strand, within rapAat 2035.448 kb on - strand, within rapAat 2035.626 kb on + strand, within rapAat 2035.721 kb on + strand, within rapAat 2035.721 kb on + strand, within rapAat 2035.722 kb on - strand, within rapAat 2035.722 kb on - strand, within rapAat 2035.880 kb on + strand, within rapAat 2035.889 kb on - strand, within rapAat 2036.080 kb on + strand, within rapAat 2036.303 kb on - strand, within rapAat 2036.366 kb on - strand, within rapAat 2036.410 kb on + strand, within rapAat 2036.410 kb on + strand, within rapAat 2036.411 kb on - strand, within rapAat 2036.435 kb on - strand, within rapAat 2036.560 kb on - strand, within rapAat 2036.605 kb on + strand, within rapAat 2036.605 kb on + strand, within rapAat 2036.605 kb on + strand, within rapAat 2036.605 kb on + strand, within rapAat 2036.606 kb on - strand, within rapAat 2036.606 kb on - strand, within rapAat 2036.606 kb on - strand, within rapAat 2036.606 kb on - strand, within rapAat 2036.670 kb on + strand, within rapAat 2036.670 kb on + strand, within rapAat 2036.670 kb on + strand, within rapAat 2036.671 kb on - strand, within rapAat 2036.671 kb on - strand, within rapAat 2036.705 kb on - strand, within rapAat 2036.761 kb on - strand, within rapAat 2036.773 kb on + strand, within rapAat 2036.828 kb on + strand, within rapAat 2036.829 kb on - strand, within rapAat 2036.931 kb on + strand, within rapAat 2036.931 kb on + strand, within rapAat 2036.933 kb on + strand, within rapAat 2036.947 kb on + strand, within rapAat 2036.952 kb on - strand, within rapAat 2037.230 kb on - strand, within rapAat 2037.230 kb on - strand, within rapAat 2037.230 kb on - strand, within rapAat 2037.326 kb on - strand, within rapAat 2037.452 kb on + strand, within rapAat 2037.452 kb on + strand, within rapAat 2037.453 kb on - strand, within rapAat 2037.453 kb on - strand, within rapAat 2037.520 kb on + strand, within rapAat 2037.521 kb on - strand, within rapAat 2037.521 kb on - strand, within rapAat 2037.589 kb on + strand, within rapAat 2037.831 kb on + strandat 2037.978 kb on + strandat 2037.978 kb on + strandat 2037.978 kb on + strandat 2037.981 kb on + strand, within rluAat 2037.981 kb on + strand, within rluAat 2037.982 kb on - strand, within rluAat 2038.127 kb on + strand, within rluAat 2038.127 kb on + strand, within rluAat 2038.199 kb on - strand, within rluAat 2038.327 kb on + strand, within rluAat 2038.413 kb on + strand, within rluAat 2038.413 kb on + strand, within rluAat 2038.453 kb on + strand, within rluAat 2038.453 kb on + strand, within rluAat 2038.454 kb on - strand, within rluAat 2038.496 kb on + strand, within rluAat 2038.496 kb on + strand, within rluAat 2038.497 kb on - strand, within rluAat 2038.497 kb on - strand, within rluAat 2038.497 kb on - strand, within rluAat 2038.497 kb on - strand, within rluAat 2038.539 kb on + strandat 2038.540 kb on - strandat 2038.543 kb on - strandat 2038.582 kb on + strandat 2038.583 kb on - strandat 2038.589 kb on - strandat 2038.618 kb on - strandat 2038.618 kb on - strandat 2038.622 kb on - strandat 2038.622 kb on - strandat 2038.760 kb on + strandat 2038.772 kb on + strandat 2038.814 kb on + strandat 2038.815 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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2,034,106 + polB OKFHMN_10795 0.69 +0.0
2,034,260 - polB OKFHMN_10795 0.76 +0.0
2,034,373 - polB OKFHMN_10795 0.80 -0.0
2,034,437 - polB OKFHMN_10795 0.83 -0.1
2,034,444 + polB OKFHMN_10795 0.84 -0.5
2,034,445 - polB OKFHMN_10795 0.84 -0.2
2,034,473 - polB OKFHMN_10795 0.85 +0.0
2,034,529 + polB OKFHMN_10795 0.87 -1.1
2,034,587 + polB OKFHMN_10795 0.90 -0.9
2,034,664 - +0.8
2,034,667 + +0.0
2,034,668 - -0.1
2,034,668 - +0.3
2,034,671 + +0.4
2,034,671 + +0.4
2,034,671 + +0.6
2,034,725 - +0.1
2,034,733 + -0.8
2,034,749 + +0.1
2,034,749 + +0.6
2,034,771 - -0.6
2,034,804 + -0.3
2,034,804 + -0.7
2,034,804 + -0.6
2,034,804 + -1.5
2,034,805 - -0.4
2,034,805 - -0.0
2,034,825 - -0.3
2,034,939 + +0.4
2,034,939 + +1.4
2,034,939 + +0.8
2,034,940 - +1.6
2,034,972 + +1.4
2,034,973 - +4.1
2,034,978 + +1.2
2,034,979 - +1.2
2,034,980 + +0.5
2,034,981 - +1.3
2,034,994 + +1.3
2,034,995 - +1.1
2,034,995 - +1.8
2,034,995 - +1.6
2,034,995 - +0.9
2,034,995 - +1.0
2,035,003 + +2.3
2,035,004 - +1.3
2,035,102 + +1.5
2,035,102 + +0.6
2,035,122 - +2.1
2,035,172 - +0.6
2,035,177 + +1.1
2,035,241 + +1.2
2,035,242 - +1.3
2,035,361 - rapA OKFHMN_10800 0.13 +1.6
2,035,393 + rapA OKFHMN_10800 0.14 +0.6
2,035,393 + rapA OKFHMN_10800 0.14 +2.5
2,035,394 - rapA OKFHMN_10800 0.14 +1.4
2,035,447 + rapA OKFHMN_10800 0.16 +0.6
2,035,448 - rapA OKFHMN_10800 0.16 +0.9
2,035,626 + rapA OKFHMN_10800 0.22 +1.0
2,035,721 + rapA OKFHMN_10800 0.25 -1.7
2,035,721 + rapA OKFHMN_10800 0.25 +1.0
2,035,722 - rapA OKFHMN_10800 0.25 +0.4
2,035,722 - rapA OKFHMN_10800 0.25 +1.4
2,035,880 + rapA OKFHMN_10800 0.30 +1.8
2,035,889 - rapA OKFHMN_10800 0.31 +1.0
2,036,080 + rapA OKFHMN_10800 0.37 +1.7
2,036,303 - rapA OKFHMN_10800 0.45 +3.1
2,036,366 - rapA OKFHMN_10800 0.47 +1.6
2,036,410 + rapA OKFHMN_10800 0.49 +1.2
2,036,410 + rapA OKFHMN_10800 0.49 +1.8
2,036,411 - rapA OKFHMN_10800 0.49 +0.7
2,036,435 - rapA OKFHMN_10800 0.50 +0.2
2,036,560 - rapA OKFHMN_10800 0.54 +1.2
2,036,605 + rapA OKFHMN_10800 0.55 +1.6
2,036,605 + rapA OKFHMN_10800 0.55 +1.5
2,036,605 + rapA OKFHMN_10800 0.55 +1.5
2,036,605 + rapA OKFHMN_10800 0.55 +1.0
2,036,606 - rapA OKFHMN_10800 0.55 +1.3
2,036,606 - rapA OKFHMN_10800 0.55 +0.8
2,036,606 - rapA OKFHMN_10800 0.55 +1.4
2,036,606 - rapA OKFHMN_10800 0.55 +1.7
2,036,670 + rapA OKFHMN_10800 0.58 +0.1
2,036,670 + rapA OKFHMN_10800 0.58 +0.5
2,036,670 + rapA OKFHMN_10800 0.58 +2.3
2,036,671 - rapA OKFHMN_10800 0.58 +2.2
2,036,671 - rapA OKFHMN_10800 0.58 +1.7
2,036,705 - rapA OKFHMN_10800 0.59 +2.1
2,036,761 - rapA OKFHMN_10800 0.61 +1.3
2,036,773 + rapA OKFHMN_10800 0.61 +2.0
2,036,828 + rapA OKFHMN_10800 0.63 +0.5
2,036,829 - rapA OKFHMN_10800 0.63 +1.5
2,036,931 + rapA OKFHMN_10800 0.67 +1.8
2,036,931 + rapA OKFHMN_10800 0.67 +1.3
2,036,933 + rapA OKFHMN_10800 0.67 +0.9
2,036,947 + rapA OKFHMN_10800 0.67 +0.5
2,036,952 - rapA OKFHMN_10800 0.67 +1.9
2,037,230 - rapA OKFHMN_10800 0.77 +1.8
2,037,230 - rapA OKFHMN_10800 0.77 +1.0
2,037,230 - rapA OKFHMN_10800 0.77 +0.1
2,037,326 - rapA OKFHMN_10800 0.80 +1.3
2,037,452 + rapA OKFHMN_10800 0.85 +1.5
2,037,452 + rapA OKFHMN_10800 0.85 +1.9
2,037,453 - rapA OKFHMN_10800 0.85 +1.6
2,037,453 - rapA OKFHMN_10800 0.85 +1.4
2,037,520 + rapA OKFHMN_10800 0.87 +1.0
2,037,521 - rapA OKFHMN_10800 0.87 +1.8
2,037,521 - rapA OKFHMN_10800 0.87 +1.6
2,037,589 + rapA OKFHMN_10800 0.89 +2.9
2,037,831 + +1.2
2,037,978 + +0.9
2,037,978 + +0.6
2,037,978 + +0.4
2,037,981 + rluA OKFHMN_10805 0.10 +0.3
2,037,981 + rluA OKFHMN_10805 0.10 +0.7
2,037,982 - rluA OKFHMN_10805 0.10 +1.0
2,038,127 + rluA OKFHMN_10805 0.32 -0.4
2,038,127 + rluA OKFHMN_10805 0.32 -0.6
2,038,199 - rluA OKFHMN_10805 0.43 +0.6
2,038,327 + rluA OKFHMN_10805 0.63 +0.6
2,038,413 + rluA OKFHMN_10805 0.76 +0.2
2,038,413 + rluA OKFHMN_10805 0.76 +0.4
2,038,453 + rluA OKFHMN_10805 0.82 -1.6
2,038,453 + rluA OKFHMN_10805 0.82 -0.9
2,038,454 - rluA OKFHMN_10805 0.82 +0.5
2,038,496 + rluA OKFHMN_10805 0.88 +0.7
2,038,496 + rluA OKFHMN_10805 0.88 -0.0
2,038,497 - rluA OKFHMN_10805 0.88 +0.0
2,038,497 - rluA OKFHMN_10805 0.88 +1.0
2,038,497 - rluA OKFHMN_10805 0.88 +0.9
2,038,497 - rluA OKFHMN_10805 0.88 +0.9
2,038,539 + +0.3
2,038,540 - +1.1
2,038,543 - +0.1
2,038,582 + +0.5
2,038,583 - -1.5
2,038,589 - +0.3
2,038,618 - -0.2
2,038,618 - +0.1
2,038,622 - -0.3
2,038,622 - -0.1
2,038,760 + -0.7
2,038,772 + +0.4
2,038,814 + -0.5
2,038,815 - +0.1

Or see this region's nucleotide sequence