Experiment: JK42
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rnhA and yafS overlap by 4 nucleotides yafS and gloB are separated by 33 nucleotides gloB and mltD are separated by 209 nucleotides
OKFHMN_09970: rnhA - ribonuclease HI, at 1,863,640 to 1,864,107
rnhA
OKFHMN_09975: yafS - Uncharacterized protein YafS, at 1,864,104 to 1,864,826
yafS
OKFHMN_09980: gloB - hydroxyacylglutathione hydrolase, at 1,864,860 to 1,865,615
gloB
OKFHMN_09985: mltD - murein transglycosylase D, at 1,865,825 to 1,867,045
mltD
Position (kb)
1864
1865
1866 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1863.870 kb on + strand, within rnhA at 1864.083 kb on + strand at 1864.118 kb on + strand at 1864.223 kb on + strand, within yafS at 1864.230 kb on + strand, within yafS at 1864.231 kb on - strand, within yafS at 1864.231 kb on - strand, within yafS at 1864.251 kb on - strand, within yafS at 1864.314 kb on + strand, within yafS at 1864.315 kb on - strand, within yafS at 1864.532 kb on + strand, within yafS at 1864.532 kb on + strand, within yafS at 1864.532 kb on + strand, within yafS at 1864.532 kb on + strand, within yafS at 1864.533 kb on - strand, within yafS at 1864.533 kb on - strand, within yafS at 1864.706 kb on - strand, within yafS at 1864.854 kb on + strand at 1864.854 kb on + strand at 1864.855 kb on - strand at 1864.860 kb on - strand at 1864.860 kb on - strand at 1864.875 kb on - strand at 1864.875 kb on - strand at 1864.897 kb on - strand at 1864.897 kb on - strand at 1864.897 kb on - strand at 1864.897 kb on - strand at 1864.897 kb on - strand at 1864.964 kb on - strand, within gloB at 1865.006 kb on - strand, within gloB at 1865.006 kb on - strand, within gloB at 1865.006 kb on - strand, within gloB at 1865.006 kb on - strand, within gloB at 1865.082 kb on + strand, within gloB at 1865.083 kb on - strand, within gloB at 1865.083 kb on - strand, within gloB at 1865.083 kb on - strand, within gloB at 1865.083 kb on - strand, within gloB at 1865.106 kb on - strand, within gloB at 1865.122 kb on + strand, within gloB at 1865.171 kb on - strand, within gloB at 1865.224 kb on - strand, within gloB at 1865.315 kb on - strand, within gloB at 1865.334 kb on - strand, within gloB at 1865.342 kb on - strand, within gloB at 1865.342 kb on - strand, within gloB at 1865.358 kb on + strand, within gloB at 1865.360 kb on + strand, within gloB at 1865.390 kb on + strand, within gloB at 1865.390 kb on + strand, within gloB at 1865.422 kb on - strand, within gloB at 1865.607 kb on - strand at 1865.675 kb on - strand at 1865.940 kb on + strand at 1865.987 kb on + strand, within mltD at 1865.987 kb on + strand, within mltD at 1865.988 kb on - strand, within mltD at 1865.989 kb on + strand, within mltD at 1865.989 kb on + strand, within mltD at 1865.990 kb on - strand, within mltD at 1865.990 kb on - strand, within mltD at 1865.990 kb on - strand, within mltD at 1865.990 kb on - strand, within mltD at 1865.994 kb on - strand, within mltD at 1866.022 kb on + strand, within mltD at 1866.023 kb on - strand, within mltD at 1866.046 kb on - strand, within mltD at 1866.048 kb on + strand, within mltD at 1866.048 kb on + strand, within mltD at 1866.048 kb on + strand, within mltD at 1866.054 kb on + strand, within mltD at 1866.164 kb on + strand, within mltD at 1866.164 kb on + strand, within mltD at 1866.165 kb on - strand, within mltD at 1866.269 kb on + strand, within mltD at 1866.269 kb on + strand, within mltD at 1866.270 kb on - strand, within mltD at 1866.270 kb on - strand, within mltD at 1866.271 kb on + strand, within mltD at 1866.374 kb on + strand, within mltD at 1866.374 kb on + strand, within mltD at 1866.375 kb on - strand, within mltD at 1866.432 kb on + strand, within mltD
Per-strain Table
Position Strand Gene LocusTag Fraction JK42 remove 1,863,870 + rnhA OKFHMN_09970 0.49 -0.5 1,864,083 + +0.3 1,864,118 + -0.1 1,864,223 + yafS OKFHMN_09975 0.16 -0.1 1,864,230 + yafS OKFHMN_09975 0.17 -2.1 1,864,231 - yafS OKFHMN_09975 0.18 +0.3 1,864,231 - yafS OKFHMN_09975 0.18 +2.6 1,864,251 - yafS OKFHMN_09975 0.20 +0.7 1,864,314 + yafS OKFHMN_09975 0.29 +0.5 1,864,315 - yafS OKFHMN_09975 0.29 +1.0 1,864,532 + yafS OKFHMN_09975 0.59 -0.0 1,864,532 + yafS OKFHMN_09975 0.59 -0.2 1,864,532 + yafS OKFHMN_09975 0.59 +0.6 1,864,532 + yafS OKFHMN_09975 0.59 -1.8 1,864,533 - yafS OKFHMN_09975 0.59 +1.0 1,864,533 - yafS OKFHMN_09975 0.59 +0.9 1,864,706 - yafS OKFHMN_09975 0.83 +0.3 1,864,854 + -0.0 1,864,854 + -0.4 1,864,855 - -0.6 1,864,860 - -0.4 1,864,860 - -0.7 1,864,875 - -0.3 1,864,875 - +0.4 1,864,897 - -0.0 1,864,897 - +0.8 1,864,897 - +0.1 1,864,897 - +0.4 1,864,897 - -0.3 1,864,964 - gloB OKFHMN_09980 0.14 -0.1 1,865,006 - gloB OKFHMN_09980 0.19 +0.5 1,865,006 - gloB OKFHMN_09980 0.19 +1.4 1,865,006 - gloB OKFHMN_09980 0.19 -0.0 1,865,006 - gloB OKFHMN_09980 0.19 +0.4 1,865,082 + gloB OKFHMN_09980 0.29 +0.7 1,865,083 - gloB OKFHMN_09980 0.29 -1.0 1,865,083 - gloB OKFHMN_09980 0.29 -1.2 1,865,083 - gloB OKFHMN_09980 0.29 -0.7 1,865,083 - gloB OKFHMN_09980 0.29 +1.1 1,865,106 - gloB OKFHMN_09980 0.33 -0.2 1,865,122 + gloB OKFHMN_09980 0.35 +0.1 1,865,171 - gloB OKFHMN_09980 0.41 +0.7 1,865,224 - gloB OKFHMN_09980 0.48 -0.1 1,865,315 - gloB OKFHMN_09980 0.60 -0.8 1,865,334 - gloB OKFHMN_09980 0.63 +0.4 1,865,342 - gloB OKFHMN_09980 0.64 +0.0 1,865,342 - gloB OKFHMN_09980 0.64 -0.2 1,865,358 + gloB OKFHMN_09980 0.66 -0.1 1,865,360 + gloB OKFHMN_09980 0.66 +0.3 1,865,390 + gloB OKFHMN_09980 0.70 +1.3 1,865,390 + gloB OKFHMN_09980 0.70 +0.2 1,865,422 - gloB OKFHMN_09980 0.74 +0.3 1,865,607 - +0.7 1,865,675 - -1.4 1,865,940 + +0.6 1,865,987 + mltD OKFHMN_09985 0.13 -0.5 1,865,987 + mltD OKFHMN_09985 0.13 -0.0 1,865,988 - mltD OKFHMN_09985 0.13 +2.6 1,865,989 + mltD OKFHMN_09985 0.13 -0.4 1,865,989 + mltD OKFHMN_09985 0.13 +0.4 1,865,990 - mltD OKFHMN_09985 0.14 -0.1 1,865,990 - mltD OKFHMN_09985 0.14 +0.6 1,865,990 - mltD OKFHMN_09985 0.14 +0.6 1,865,990 - mltD OKFHMN_09985 0.14 -1.3 1,865,994 - mltD OKFHMN_09985 0.14 -0.5 1,866,022 + mltD OKFHMN_09985 0.16 +0.7 1,866,023 - mltD OKFHMN_09985 0.16 -1.3 1,866,046 - mltD OKFHMN_09985 0.18 -0.8 1,866,048 + mltD OKFHMN_09985 0.18 -0.3 1,866,048 + mltD OKFHMN_09985 0.18 +1.8 1,866,048 + mltD OKFHMN_09985 0.18 -0.1 1,866,054 + mltD OKFHMN_09985 0.19 -0.4 1,866,164 + mltD OKFHMN_09985 0.28 -0.1 1,866,164 + mltD OKFHMN_09985 0.28 -0.3 1,866,165 - mltD OKFHMN_09985 0.28 +0.6 1,866,269 + mltD OKFHMN_09985 0.36 -0.2 1,866,269 + mltD OKFHMN_09985 0.36 -0.2 1,866,270 - mltD OKFHMN_09985 0.36 +1.6 1,866,270 - mltD OKFHMN_09985 0.36 -0.7 1,866,271 + mltD OKFHMN_09985 0.37 -0.0 1,866,374 + mltD OKFHMN_09985 0.45 -0.4 1,866,374 + mltD OKFHMN_09985 0.45 +0.2 1,866,375 - mltD OKFHMN_09985 0.45 -1.6 1,866,432 + mltD OKFHMN_09985 0.50 -0.2
Or see this region's nucleotide sequence