Experiment: JK42
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mhpF and mhpD overlap by 4 nucleotides mhpD and mhpC are separated by 9 nucleotides mhpC and mhpB are separated by 17 nucleotides mhpB and mhpA are separated by 1 nucleotides
OKFHMN_08765: mhpF - acetaldehyde dehydrogenase, at 1,640,533 to 1,641,483
mhpF
OKFHMN_08770: mhpD - 2-keto-4-pentenoate hydratase, at 1,641,480 to 1,642,289
mhpD
OKFHMN_08775: mhpC - 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase, at 1,642,299 to 1,643,165
mhpC
OKFHMN_08780: mhpB - 3-carboxyethylcatechol 2,3-dioxygenase, at 1,643,183 to 1,644,127
mhpB
OKFHMN_08785: mhpA - bifunctional 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase, at 1,644,129 to 1,645,793
mhpA
Position (kb)
1642
1643
1644 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1641.337 kb on - strand, within mhpF at 1641.472 kb on - strand at 1641.478 kb on - strand at 1641.534 kb on + strand at 1641.535 kb on - strand at 1641.591 kb on + strand, within mhpD at 1641.728 kb on + strand, within mhpD at 1641.729 kb on - strand, within mhpD at 1641.729 kb on - strand, within mhpD at 1641.799 kb on + strand, within mhpD at 1641.892 kb on - strand, within mhpD at 1641.910 kb on + strand, within mhpD at 1642.044 kb on + strand, within mhpD at 1642.113 kb on - strand, within mhpD at 1642.147 kb on + strand, within mhpD at 1642.158 kb on + strand, within mhpD at 1642.159 kb on - strand, within mhpD at 1642.159 kb on - strand, within mhpD at 1642.252 kb on - strand at 1642.277 kb on + strand at 1642.278 kb on - strand at 1642.385 kb on + strand at 1642.386 kb on - strand, within mhpC at 1642.641 kb on - strand, within mhpC at 1642.703 kb on + strand, within mhpC at 1642.811 kb on + strand, within mhpC at 1642.812 kb on - strand, within mhpC at 1642.914 kb on - strand, within mhpC at 1642.995 kb on - strand, within mhpC at 1643.082 kb on - strand at 1643.082 kb on - strand at 1643.116 kb on - strand at 1643.247 kb on + strand at 1643.247 kb on + strand at 1643.612 kb on + strand, within mhpB at 1643.613 kb on - strand, within mhpB at 1643.629 kb on + strand, within mhpB at 1643.798 kb on - strand, within mhpB at 1643.798 kb on - strand, within mhpB at 1643.952 kb on + strand, within mhpB at 1644.060 kb on - strand at 1644.118 kb on - strand at 1644.118 kb on - strand at 1644.118 kb on - strand at 1644.135 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction JK42 remove 1,641,337 - mhpF OKFHMN_08765 0.85 +0.5 1,641,472 - +0.5 1,641,478 - +2.5 1,641,534 + -0.6 1,641,535 - -0.0 1,641,591 + mhpD OKFHMN_08770 0.14 +0.4 1,641,728 + mhpD OKFHMN_08770 0.31 +0.0 1,641,729 - mhpD OKFHMN_08770 0.31 -1.2 1,641,729 - mhpD OKFHMN_08770 0.31 -0.2 1,641,799 + mhpD OKFHMN_08770 0.39 -0.8 1,641,892 - mhpD OKFHMN_08770 0.51 -0.1 1,641,910 + mhpD OKFHMN_08770 0.53 -0.3 1,642,044 + mhpD OKFHMN_08770 0.70 -0.4 1,642,113 - mhpD OKFHMN_08770 0.78 -2.1 1,642,147 + mhpD OKFHMN_08770 0.82 +0.9 1,642,158 + mhpD OKFHMN_08770 0.84 +2.5 1,642,159 - mhpD OKFHMN_08770 0.84 +1.4 1,642,159 - mhpD OKFHMN_08770 0.84 -0.7 1,642,252 - +0.6 1,642,277 + -0.6 1,642,278 - -0.7 1,642,385 + +0.4 1,642,386 - mhpC OKFHMN_08775 0.10 -0.3 1,642,641 - mhpC OKFHMN_08775 0.39 -0.2 1,642,703 + mhpC OKFHMN_08775 0.47 -0.7 1,642,811 + mhpC OKFHMN_08775 0.59 +0.6 1,642,812 - mhpC OKFHMN_08775 0.59 -0.3 1,642,914 - mhpC OKFHMN_08775 0.71 +0.1 1,642,995 - mhpC OKFHMN_08775 0.80 +0.2 1,643,082 - -0.2 1,643,082 - -0.2 1,643,116 - +1.1 1,643,247 + -0.6 1,643,247 + -0.2 1,643,612 + mhpB OKFHMN_08780 0.45 -0.3 1,643,613 - mhpB OKFHMN_08780 0.46 -0.1 1,643,629 + mhpB OKFHMN_08780 0.47 +0.3 1,643,798 - mhpB OKFHMN_08780 0.65 +0.5 1,643,798 - mhpB OKFHMN_08780 0.65 -0.0 1,643,952 + mhpB OKFHMN_08780 0.81 -1.1 1,644,060 - -0.0 1,644,118 - +1.5 1,644,118 - +1.0 1,644,118 - -0.0 1,644,135 + +0.2
Or see this region's nucleotide sequence