Strain Fitness in Escherichia coli ECRC100 around OKFHMN_05375

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpncB and asnS are separated by 168 nucleotidesasnS and ompF are separated by 602 nucleotides OKFHMN_05370: pncB - nicotinate phosphoribosyltransferase, at 896,341 to 897,543 pncB OKFHMN_05375: asnS - asparagine--tRNA ligase, at 897,712 to 899,112 asnS OKFHMN_05380: ompF - porin OmpF, at 899,715 to 900,803 ompF Position (kb) 897 898 899 900Strain fitness (log2 ratio) -2 -1 0 1at 896.835 kb on + strand, within pncBat 896.835 kb on + strand, within pncBat 896.836 kb on - strand, within pncBat 896.950 kb on + strand, within pncBat 897.312 kb on - strand, within pncBat 897.399 kb on + strand, within pncBat 897.408 kb on + strand, within pncBat 897.525 kb on + strandat 897.541 kb on + strandat 897.541 kb on + strandat 899.110 kb on + strandat 899.110 kb on + strandat 899.122 kb on + strandat 899.713 kb on + strandat 899.714 kb on - strandat 899.761 kb on + strandat 899.898 kb on + strand, within ompFat 899.969 kb on + strand, within ompFat 899.969 kb on + strand, within ompFat 900.085 kb on - strand, within ompF

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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896,835 + pncB OKFHMN_05370 0.41 -0.5
896,835 + pncB OKFHMN_05370 0.41 -1.0
896,836 - pncB OKFHMN_05370 0.41 -0.6
896,950 + pncB OKFHMN_05370 0.51 -0.6
897,312 - pncB OKFHMN_05370 0.81 -1.5
897,399 + pncB OKFHMN_05370 0.88 +0.0
897,408 + pncB OKFHMN_05370 0.89 -0.4
897,525 + -0.3
897,541 + +0.3
897,541 + +0.0
899,110 + +0.0
899,110 + +1.1
899,122 + -0.1
899,713 + -0.3
899,714 - -0.7
899,761 + -0.8
899,898 + ompF OKFHMN_05380 0.17 +0.7
899,969 + ompF OKFHMN_05380 0.23 -2.1
899,969 + ompF OKFHMN_05380 0.23 -0.6
900,085 - ompF OKFHMN_05380 0.34 -0.9

Or see this region's nucleotide sequence