Strain Fitness in Escherichia coli ECRC100 around OKFHMN_04300

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntureC and ureB overlap by 8 nucleotidesureB and ureA are separated by 8 nucleotidesureA and ureD1 are separated by 90 nucleotidesureD1 and OKFHMN_04305 are separated by 380 nucleotidesOKFHMN_04305 and opgE are separated by 224 nucleotides OKFHMN_04280: ureC - urease subunit alpha, at 683,817 to 685,520 ureC OKFHMN_04290: ureB - urease subunit beta, at 685,513 to 685,833 ureB OKFHMN_04295: ureA - urease subunit gamma, at 685,842 to 686,144 ureA OKFHMN_04300: ureD1 - Putative urease accessory UreD-like protein, at 686,235 to 686,933 ureD1 OKFHMN_04305: OKFHMN_04305 - hypothetical protein, at 687,314 to 687,589 _04305 OKFHMN_04310: opgE - phosphoethanolamine transferase, at 687,814 to 689,433 opgE Position (kb) 686 687Strain fitness (log2 ratio) -1 0 1 2at 685.410 kb on - strandat 685.543 kb on + strandat 685.546 kb on - strand, within ureBat 685.546 kb on - strand, within ureBat 685.774 kb on - strand, within ureBat 685.796 kb on - strand, within ureBat 685.800 kb on - strand, within ureBat 685.818 kb on - strandat 685.818 kb on - strandat 685.927 kb on - strand, within ureAat 686.018 kb on + strand, within ureAat 686.110 kb on - strand, within ureAat 686.112 kb on + strand, within ureAat 686.155 kb on + strandat 686.233 kb on - strandat 686.253 kb on - strandat 686.338 kb on - strand, within ureD1at 687.038 kb on - strandat 687.080 kb on - strandat 687.125 kb on + strandat 687.126 kb on - strandat 687.236 kb on + strandat 687.266 kb on + strandat 687.434 kb on - strand, within OKFHMN_04305

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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685,410 - +0.3
685,543 + +1.9
685,546 - ureB OKFHMN_04290 0.10 +0.6
685,546 - ureB OKFHMN_04290 0.10 -1.3
685,774 - ureB OKFHMN_04290 0.81 +1.3
685,796 - ureB OKFHMN_04290 0.88 +0.4
685,800 - ureB OKFHMN_04290 0.89 -1.5
685,818 - -0.3
685,818 - +0.8
685,927 - ureA OKFHMN_04295 0.28 -0.3
686,018 + ureA OKFHMN_04295 0.58 -0.3
686,110 - ureA OKFHMN_04295 0.88 -0.5
686,112 + ureA OKFHMN_04295 0.89 +0.9
686,155 + +1.3
686,233 - -0.6
686,253 - +0.1
686,338 - ureD1 OKFHMN_04300 0.15 +0.3
687,038 - -0.1
687,080 - +1.3
687,125 + +0.1
687,126 - +0.4
687,236 + +0.7
687,266 + +2.3
687,434 - OKFHMN_04305 0.43 -1.3

Or see this region's nucleotide sequence