Strain Fitness in Escherichia coli ECRC100 around OKFHMN_03075

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_03065 and OKFHMN_03070 overlap by 4 nucleotidesOKFHMN_03070 and OKFHMN_03075 are separated by 92 nucleotidesOKFHMN_03075 and OKFHMN_03080 overlap by 4 nucleotidesOKFHMN_03080 and OKFHMN_03085 are separated by 105 nucleotides OKFHMN_03065: OKFHMN_03065 - Cell division inhibitor, at 485,665 to 485,853 _03065 OKFHMN_03070: OKFHMN_03070 - DUF1482 domain-containing protein, at 485,850 to 486,038 _03070 OKFHMN_03075: OKFHMN_03075 - Exonuclease, at 486,131 to 487,375 _03075 OKFHMN_03080: OKFHMN_03080 - site-specific integrase, at 487,372 to 487,908 _03080 OKFHMN_03085: OKFHMN_03085 - DinI-like protein Z2083/ECs2153, at 488,014 to 488,328 _03085 Position (kb) 486 487 488Strain fitness (log2 ratio) -1 0 1 2at 486.159 kb on + strandat 486.413 kb on - strand, within OKFHMN_03075at 486.622 kb on + strand, within OKFHMN_03075at 486.797 kb on + strand, within OKFHMN_03075at 486.798 kb on - strand, within OKFHMN_03075at 487.002 kb on - strand, within OKFHMN_03075at 487.163 kb on + strand, within OKFHMN_03075at 487.711 kb on + strand, within OKFHMN_03080

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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486,159 + +1.9
486,413 - OKFHMN_03075 0.23 +0.3
486,622 + OKFHMN_03075 0.39 +0.3
486,797 + OKFHMN_03075 0.53 -0.1
486,798 - OKFHMN_03075 0.54 -1.3
487,002 - OKFHMN_03075 0.70 +1.3
487,163 + OKFHMN_03075 0.83 -0.5
487,711 + OKFHMN_03080 0.63 +1.3

Or see this region's nucleotide sequence