Strain Fitness in Escherichia coli ECRC100 around OKFHMN_02125

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_02100 and kilR are separated by 46 nucleotideskilR and OKFHMN_02120 overlap by 1 nucleotidesOKFHMN_02120 and recE are separated by 80 nucleotidesrecE and recT overlap by 8 nucleotides OKFHMN_02100: OKFHMN_02100 - transcriptional regulator, at 316,859 to 317,707 _02100 OKFHMN_02115: kilR - killing protein KilR, at 317,754 to 317,975 kilR OKFHMN_02120: OKFHMN_02120 - Rtr1/RPAP2 family, at 317,975 to 318,145 _02120 OKFHMN_02125: recE - exodeoxyribonuclease VIII, at 318,226 to 320,895 recE OKFHMN_02130: recT - recombination protein RecT, at 320,888 to 321,697 recT Position (kb) 318 319 320 321Strain fitness (log2 ratio) -3 -2 -1 0 1at 317.488 kb on + strand, within OKFHMN_02100at 319.000 kb on - strand, within recEat 319.912 kb on + strand, within recEat 319.950 kb on + strand, within recEat 320.088 kb on + strand, within recEat 320.294 kb on + strand, within recEat 320.844 kb on + strandat 320.844 kb on + strandat 320.844 kb on + strandat 320.844 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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317,488 + OKFHMN_02100 0.74 +0.6
319,000 - recE OKFHMN_02125 0.29 -0.3
319,912 + recE OKFHMN_02125 0.63 -3.0
319,950 + recE OKFHMN_02125 0.65 -1.9
320,088 + recE OKFHMN_02125 0.70 -1.5
320,294 + recE OKFHMN_02125 0.77 -2.2
320,844 + -1.0
320,844 + -3.1
320,844 + +0.3
320,844 + -1.0

Or see this region's nucleotide sequence