Strain Fitness in Escherichia coli ECRC100 around OKFHMN_01800

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntldhA and hslJ are separated by 110 nucleotideshslJ and ydbJ overlap by 4 nucleotidesydbJ and nifJ are separated by 273 nucleotides OKFHMN_01795: ldhA - D-lactate dehydrogenase, at 270,565 to 271,554 ldhA OKFHMN_01800: hslJ - heat shock protein HslJ, at 271,665 to 272,087 hslJ OKFHMN_01805: ydbJ - Uncharacterized protein YdbJ, at 272,084 to 272,350 ydbJ OKFHMN_01810: nifJ - pyruvate:ferredoxin (flavodoxin) oxidoreductase, at 272,624 to 276,148 nifJ Position (kb) 271 272 273Strain fitness (log2 ratio) -1 0 1 2at 270.988 kb on - strand, within ldhAat 270.991 kb on + strand, within ldhAat 270.992 kb on - strand, within ldhAat 271.065 kb on + strand, within ldhAat 271.594 kb on + strandat 271.753 kb on + strand, within hslJat 271.959 kb on - strand, within hslJat 272.064 kb on - strandat 272.262 kb on - strand, within ydbJat 272.395 kb on - strandat 272.612 kb on + strandat 272.624 kb on - strandat 272.943 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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270,988 - ldhA OKFHMN_01795 0.43 -1.7
270,991 + ldhA OKFHMN_01795 0.43 -1.6
270,992 - ldhA OKFHMN_01795 0.43 +1.3
271,065 + ldhA OKFHMN_01795 0.51 +0.9
271,594 + +1.3
271,753 + hslJ OKFHMN_01800 0.21 +1.3
271,959 - hslJ OKFHMN_01800 0.70 -0.4
272,064 - -0.0
272,262 - ydbJ OKFHMN_01805 0.67 +2.3
272,395 - +2.6
272,612 + -1.0
272,624 - +0.4
272,943 + -0.8

Or see this region's nucleotide sequence