Strain Fitness in Escherichia coli ECRC100 around OKFHMN_01115

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsad and glsB are separated by 63 nucleotidesglsB and yneG overlap by 1 nucleotidesyneG and gGDEF are separated by 138 nucleotides OKFHMN_01110: sad - succinate-semialdehyde dehydrogenase, at 109,493 to 110,881 sad OKFHMN_01115: glsB - glutaminase B, at 110,945 to 111,871 glsB OKFHMN_01120: yneG - Uncharacterized protein YneG, at 111,871 to 112,230 yneG OKFHMN_01125: gGDEF - diguanylate cyclase, at 112,369 to 113,787 gGDEF Position (kb) 110 111 112Strain fitness (log2 ratio) -1 0 1at 109.962 kb on - strand, within sadat 109.965 kb on + strand, within sadat 109.969 kb on + strand, within sadat 110.046 kb on + strand, within sadat 110.048 kb on + strand, within sadat 110.048 kb on + strand, within sadat 111.355 kb on + strand, within glsBat 112.178 kb on - strand, within yneGat 112.253 kb on - strandat 112.273 kb on - strandat 112.322 kb on - strandat 112.426 kb on - strandat 112.662 kb on + strand, within gGDEFat 112.671 kb on + strand, within gGDEFat 112.755 kb on + strand, within gGDEF

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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109,962 - sad OKFHMN_01110 0.34 -0.4
109,965 + sad OKFHMN_01110 0.34 +0.5
109,969 + sad OKFHMN_01110 0.34 -0.2
110,046 + sad OKFHMN_01110 0.40 -0.9
110,048 + sad OKFHMN_01110 0.40 +0.7
110,048 + sad OKFHMN_01110 0.40 +1.5
111,355 + glsB OKFHMN_01115 0.44 +0.3
112,178 - yneG OKFHMN_01120 0.85 -0.0
112,253 - -1.2
112,273 - -0.1
112,322 - -0.6
112,426 - +1.0
112,662 + gGDEF OKFHMN_01125 0.21 -0.6
112,671 + gGDEF OKFHMN_01125 0.21 -0.3
112,755 + gGDEF OKFHMN_01125 0.27 -0.5

Or see this region's nucleotide sequence