Strain Fitness in Escherichia coli ECRC100 around OKFHMN_00715

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_00700 and OKFHMN_00705 are separated by 12 nucleotidesOKFHMN_00705 and OKFHMN_00710 are separated by 12 nucleotidesOKFHMN_00710 and OKFHMN_00715 are separated by 205 nucleotidesOKFHMN_00715 and OKFHMN_00720 are separated by 255 nucleotides OKFHMN_00700: OKFHMN_00700 - capsid protein, at 42,776 to 43,831 _00700 OKFHMN_00705: OKFHMN_00705 - head decoration protein, at 43,844 to 44,179 _00705 OKFHMN_00710: OKFHMN_00710 - phage head-tail connector protein, at 44,192 to 44,605 _00710 OKFHMN_00715: OKFHMN_00715 - terminase, at 44,811 to 45,353 _00715 OKFHMN_00720: OKFHMN_00720 - hypothetical protein, at 45,609 to 45,890 _00720 Position (kb) 44 45 46Strain fitness (log2 ratio) -2 -1 0 1 2 3at 43.844 kb on - strandat 44.282 kb on + strand, within OKFHMN_00710at 44.282 kb on + strand, within OKFHMN_00710at 44.369 kb on + strand, within OKFHMN_00710at 44.407 kb on + strand, within OKFHMN_00710at 44.407 kb on + strand, within OKFHMN_00710at 44.448 kb on + strand, within OKFHMN_00710at 44.448 kb on + strand, within OKFHMN_00710at 44.482 kb on + strand, within OKFHMN_00710at 44.546 kb on + strand, within OKFHMN_00710at 44.796 kb on + strandat 44.807 kb on - strandat 44.906 kb on + strand, within OKFHMN_00715at 45.625 kb on + strandat 46.187 kb on + strandat 46.349 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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43,844 - -1.3
44,282 + OKFHMN_00710 0.22 +2.3
44,282 + OKFHMN_00710 0.22 +1.9
44,369 + OKFHMN_00710 0.43 +2.5
44,407 + OKFHMN_00710 0.52 +1.3
44,407 + OKFHMN_00710 0.52 +2.3
44,448 + OKFHMN_00710 0.62 +1.0
44,448 + OKFHMN_00710 0.62 +3.3
44,482 + OKFHMN_00710 0.70 +0.8
44,546 + OKFHMN_00710 0.86 +2.0
44,796 + +0.2
44,807 - +0.5
44,906 + OKFHMN_00715 0.17 -0.1
45,625 + +0.3
46,187 + +0.3
46,349 + -2.0

Or see this region's nucleotide sequence