Strain Fitness in Sphingobium sp. HT1-2 around GFF61

Experiment: EMM_noCarbon with Sucrose and Ammonium Sulfate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF60 and GFF61 are separated by 46 nucleotidesGFF61 and GFF62 are separated by 139 nucleotidesGFF62 and GFF63 are separated by 105 nucleotidesGFF63 and GFF64 are separated by 93 nucleotidesGFF64 and GFF65 are separated by 120 nucleotides GFF60 - Pyruvate,phosphate dikinase (EC 2.7.9.1), at 50,420 to 53,119 GFF60 GFF61 - hypothetical protein, at 53,166 to 53,657 GFF61 GFF62 - tRNA-Arg-ACG, at 53,797 to 53,873 GFF62 GFF63 - hypothetical protein, at 53,979 to 54,158 GFF63 GFF64 - hypothetical protein, at 54,252 to 54,434 GFF64 GFF65 - tRNA-Arg-ACG, at 54,555 to 54,631 GFF65 Position (kb) 53 54Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 52.168 kb on - strand, within GFF60at 52.171 kb on + strand, within GFF60at 52.171 kb on + strand, within GFF60at 52.171 kb on + strand, within GFF60at 52.171 kb on + strand, within GFF60at 52.171 kb on + strand, within GFF60at 52.171 kb on + strand, within GFF60at 52.171 kb on + strand, within GFF60at 52.171 kb on + strand, within GFF60at 52.171 kb on + strand, within GFF60at 52.174 kb on - strand, within GFF60at 52.174 kb on - strand, within GFF60at 52.179 kb on - strand, within GFF60at 52.179 kb on - strand, within GFF60at 52.179 kb on - strand, within GFF60at 52.179 kb on - strand, within GFF60at 52.179 kb on - strand, within GFF60at 52.181 kb on + strand, within GFF60at 52.202 kb on + strand, within GFF60at 52.209 kb on + strand, within GFF60at 52.210 kb on - strand, within GFF60at 52.217 kb on - strand, within GFF60at 52.224 kb on + strand, within GFF60at 52.224 kb on + strand, within GFF60at 52.224 kb on + strand, within GFF60at 52.282 kb on - strand, within GFF60at 52.316 kb on - strand, within GFF60at 52.320 kb on + strand, within GFF60at 52.328 kb on - strand, within GFF60at 52.338 kb on + strand, within GFF60at 52.338 kb on - strand, within GFF60at 52.338 kb on - strand, within GFF60at 52.338 kb on - strand, within GFF60at 52.338 kb on - strand, within GFF60at 52.339 kb on - strand, within GFF60at 52.346 kb on - strand, within GFF60at 52.346 kb on - strand, within GFF60at 52.348 kb on + strand, within GFF60at 52.405 kb on + strand, within GFF60at 52.405 kb on + strand, within GFF60at 52.405 kb on + strand, within GFF60at 52.418 kb on + strand, within GFF60at 52.425 kb on + strand, within GFF60at 52.425 kb on + strand, within GFF60at 52.457 kb on - strand, within GFF60at 52.483 kb on - strand, within GFF60at 52.502 kb on + strand, within GFF60at 52.510 kb on - strand, within GFF60at 52.512 kb on + strand, within GFF60at 52.546 kb on + strand, within GFF60at 52.552 kb on - strand, within GFF60at 52.554 kb on - strand, within GFF60at 52.576 kb on + strand, within GFF60at 52.601 kb on + strand, within GFF60at 52.634 kb on - strand, within GFF60at 52.642 kb on + strand, within GFF60at 52.642 kb on + strand, within GFF60at 52.750 kb on - strand, within GFF60at 52.794 kb on + strand, within GFF60at 52.794 kb on - strand, within GFF60at 52.835 kb on - strand, within GFF60at 52.914 kb on + strandat 52.914 kb on + strandat 52.927 kb on - strandat 52.986 kb on + strandat 53.062 kb on - strandat 53.064 kb on + strandat 53.139 kb on + strandat 53.164 kb on + strandat 53.164 kb on + strandat 53.164 kb on + strandat 53.164 kb on + strandat 53.195 kb on + strandat 53.244 kb on - strand, within GFF61at 53.307 kb on + strand, within GFF61at 53.414 kb on - strand, within GFF61at 53.517 kb on - strand, within GFF61at 53.605 kb on + strand, within GFF61at 53.622 kb on - strandat 53.630 kb on + strandat 53.712 kb on + strandat 53.812 kb on - strand, within GFF62at 53.907 kb on + strandat 53.981 kb on + strandat 53.988 kb on + strandat 53.988 kb on + strandat 53.988 kb on + strandat 53.996 kb on - strandat 54.010 kb on + strand, within GFF63at 54.035 kb on + strand, within GFF63at 54.043 kb on - strand, within GFF63at 54.044 kb on - strand, within GFF63at 54.075 kb on + strand, within GFF63at 54.092 kb on + strand, within GFF63at 54.105 kb on + strand, within GFF63at 54.105 kb on + strand, within GFF63at 54.113 kb on - strand, within GFF63at 54.113 kb on - strand, within GFF63at 54.119 kb on + strand, within GFF63at 54.127 kb on - strand, within GFF63at 54.159 kb on + strandat 54.198 kb on + strandat 54.259 kb on + strandat 54.262 kb on - strandat 54.301 kb on - strand, within GFF64at 54.337 kb on - strand, within GFF64at 54.369 kb on + strand, within GFF64at 54.428 kb on + strandat 54.548 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Ammonium Sulfate
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52,168 - GFF60 0.65 -1.6
52,171 + GFF60 0.65 -0.5
52,171 + GFF60 0.65 -2.2
52,171 + GFF60 0.65 -0.6
52,171 + GFF60 0.65 -1.4
52,171 + GFF60 0.65 -1.0
52,171 + GFF60 0.65 +0.1
52,171 + GFF60 0.65 +0.1
52,171 + GFF60 0.65 -1.9
52,171 + GFF60 0.65 -2.1
52,174 - GFF60 0.65 -0.1
52,174 - GFF60 0.65 -1.4
52,179 - GFF60 0.65 -1.0
52,179 - GFF60 0.65 -0.7
52,179 - GFF60 0.65 -1.4
52,179 - GFF60 0.65 -2.0
52,179 - GFF60 0.65 -2.0
52,181 + GFF60 0.65 -1.2
52,202 + GFF60 0.66 -3.3
52,209 + GFF60 0.66 +0.7
52,210 - GFF60 0.66 -1.1
52,217 - GFF60 0.67 -1.4
52,224 + GFF60 0.67 -2.1
52,224 + GFF60 0.67 -0.6
52,224 + GFF60 0.67 -1.5
52,282 - GFF60 0.69 -0.2
52,316 - GFF60 0.70 -2.1
52,320 + GFF60 0.70 -2.0
52,328 - GFF60 0.71 -0.9
52,338 + GFF60 0.71 -1.3
52,338 - GFF60 0.71 -1.0
52,338 - GFF60 0.71 +0.8
52,338 - GFF60 0.71 -1.9
52,338 - GFF60 0.71 -2.8
52,339 - GFF60 0.71 -1.5
52,346 - GFF60 0.71 -1.8
52,346 - GFF60 0.71 -1.8
52,348 + GFF60 0.71 -3.3
52,405 + GFF60 0.74 -1.4
52,405 + GFF60 0.74 -1.8
52,405 + GFF60 0.74 -0.2
52,418 + GFF60 0.74 -1.6
52,425 + GFF60 0.74 -0.4
52,425 + GFF60 0.74 -2.3
52,457 - GFF60 0.75 -2.5
52,483 - GFF60 0.76 -2.0
52,502 + GFF60 0.77 -2.4
52,510 - GFF60 0.77 -2.5
52,512 + GFF60 0.77 -1.5
52,546 + GFF60 0.79 +0.5
52,552 - GFF60 0.79 -2.4
52,554 - GFF60 0.79 -0.7
52,576 + GFF60 0.80 +0.3
52,601 + GFF60 0.81 -1.6
52,634 - GFF60 0.82 -0.2
52,642 + GFF60 0.82 +0.8
52,642 + GFF60 0.82 -0.3
52,750 - GFF60 0.86 -1.5
52,794 + GFF60 0.88 +0.1
52,794 - GFF60 0.88 -1.4
52,835 - GFF60 0.89 -1.0
52,914 + -2.0
52,914 + +1.4
52,927 - -2.3
52,986 + -2.4
53,062 - -1.4
53,064 + -1.3
53,139 + -1.6
53,164 + -0.2
53,164 + +0.7
53,164 + -1.7
53,164 + +0.0
53,195 + -3.5
53,244 - GFF61 0.16 +0.4
53,307 + GFF61 0.29 +0.8
53,414 - GFF61 0.50 +0.3
53,517 - GFF61 0.71 -0.4
53,605 + GFF61 0.89 +0.5
53,622 - +0.4
53,630 + +0.1
53,712 + -0.7
53,812 - GFF62 0.19 +0.7
53,907 + -0.9
53,981 + -0.8
53,988 + -0.2
53,988 + -0.4
53,988 + -2.0
53,996 - +0.3
54,010 + GFF63 0.17 +0.2
54,035 + GFF63 0.31 -1.5
54,043 - GFF63 0.36 -0.2
54,044 - GFF63 0.36 +0.0
54,075 + GFF63 0.53 +0.6
54,092 + GFF63 0.63 -2.2
54,105 + GFF63 0.70 +2.8
54,105 + GFF63 0.70 +0.6
54,113 - GFF63 0.74 +0.1
54,113 - GFF63 0.74 -1.3
54,119 + GFF63 0.78 +1.2
54,127 - GFF63 0.82 -0.2
54,159 + +0.4
54,198 + +0.0
54,259 + +0.7
54,262 - -0.3
54,301 - GFF64 0.27 -1.2
54,337 - GFF64 0.46 +1.1
54,369 + GFF64 0.64 -1.4
54,428 + -0.5
54,548 + -2.0

Or see this region's nucleotide sequence