Experiment: EMM_noCarbon with Sucrose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF453 and GFF454 overlap by 1 nucleotides GFF454 and GFF455 are separated by 136 nucleotides GFF455 and GFF456 overlap by 4 nucleotides
GFF453 - 3-oxosteroid 1-dehydrogenase (EC 1.3.99.4), at 451,248 to 452,966
GFF453
GFF454 - hypothetical protein, at 452,966 to 453,430
GFF454
GFF455 - putative dioxygenase ferredoxin subunit, at 453,567 to 453,896
GFF455
GFF456 - hypothetical protein, at 453,893 to 454,861
GFF456
Position (kb)
453
454 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 452.567 kb on + strand, within GFF453 at 452.575 kb on - strand, within GFF453 at 452.575 kb on - strand, within GFF453 at 452.610 kb on - strand, within GFF453 at 452.640 kb on - strand, within GFF453 at 452.640 kb on - strand, within GFF453 at 452.659 kb on + strand, within GFF453 at 452.659 kb on + strand, within GFF453 at 452.659 kb on + strand, within GFF453 at 452.664 kb on + strand, within GFF453 at 452.666 kb on + strand, within GFF453 at 452.667 kb on - strand, within GFF453 at 452.667 kb on - strand, within GFF453 at 452.672 kb on - strand, within GFF453 at 452.672 kb on - strand, within GFF453 at 452.674 kb on - strand, within GFF453 at 452.684 kb on - strand, within GFF453 at 452.692 kb on - strand, within GFF453 at 452.699 kb on + strand, within GFF453 at 452.748 kb on + strand, within GFF453 at 452.757 kb on + strand, within GFF453 at 452.776 kb on + strand, within GFF453 at 452.798 kb on + strand at 452.801 kb on - strand at 452.801 kb on - strand at 452.806 kb on - strand at 452.806 kb on - strand at 452.806 kb on - strand at 452.806 kb on - strand at 452.820 kb on - strand at 452.824 kb on - strand at 452.833 kb on - strand at 452.871 kb on + strand at 452.876 kb on + strand at 452.881 kb on + strand at 452.885 kb on + strand at 452.885 kb on + strand at 452.891 kb on + strand at 452.893 kb on - strand at 452.893 kb on - strand at 452.893 kb on - strand at 452.894 kb on - strand at 452.894 kb on - strand at 452.904 kb on - strand at 452.947 kb on + strand at 452.948 kb on + strand at 452.948 kb on + strand at 452.948 kb on + strand at 452.948 kb on + strand at 452.956 kb on - strand at 452.980 kb on - strand at 452.982 kb on + strand at 452.982 kb on + strand at 453.029 kb on + strand, within GFF454 at 453.029 kb on + strand, within GFF454 at 453.055 kb on + strand, within GFF454 at 453.055 kb on + strand, within GFF454 at 453.055 kb on + strand, within GFF454 at 453.055 kb on + strand, within GFF454 at 453.056 kb on + strand, within GFF454 at 453.063 kb on - strand, within GFF454 at 453.063 kb on - strand, within GFF454 at 453.080 kb on - strand, within GFF454 at 453.094 kb on - strand, within GFF454 at 453.099 kb on - strand, within GFF454 at 453.099 kb on - strand, within GFF454 at 453.104 kb on + strand, within GFF454 at 453.107 kb on - strand, within GFF454 at 453.112 kb on - strand, within GFF454 at 453.127 kb on + strand, within GFF454 at 453.128 kb on + strand, within GFF454 at 453.178 kb on + strand, within GFF454 at 453.186 kb on - strand, within GFF454 at 453.186 kb on - strand, within GFF454 at 453.186 kb on - strand, within GFF454 at 453.186 kb on - strand, within GFF454 at 453.203 kb on + strand, within GFF454 at 453.233 kb on + strand, within GFF454 at 453.233 kb on + strand, within GFF454 at 453.241 kb on - strand, within GFF454 at 453.242 kb on - strand, within GFF454 at 453.242 kb on - strand, within GFF454 at 453.251 kb on + strand, within GFF454 at 453.251 kb on - strand, within GFF454 at 453.251 kb on - strand, within GFF454 at 453.256 kb on + strand, within GFF454 at 453.259 kb on - strand, within GFF454 at 453.264 kb on - strand, within GFF454 at 453.287 kb on - strand, within GFF454 at 453.299 kb on + strand, within GFF454 at 453.301 kb on + strand, within GFF454 at 453.307 kb on - strand, within GFF454 at 453.307 kb on - strand, within GFF454 at 453.307 kb on - strand, within GFF454 at 453.309 kb on + strand, within GFF454 at 453.309 kb on + strand, within GFF454 at 453.309 kb on + strand, within GFF454 at 453.309 kb on + strand, within GFF454 at 453.309 kb on + strand, within GFF454 at 453.309 kb on + strand, within GFF454 at 453.309 kb on + strand, within GFF454 at 453.309 kb on + strand, within GFF454 at 453.309 kb on + strand, within GFF454 at 453.317 kb on - strand, within GFF454 at 453.317 kb on - strand, within GFF454 at 453.317 kb on - strand, within GFF454 at 453.317 kb on - strand, within GFF454 at 453.317 kb on - strand, within GFF454 at 453.317 kb on - strand, within GFF454 at 453.319 kb on - strand, within GFF454 at 453.319 kb on - strand, within GFF454 at 453.358 kb on - strand, within GFF454 at 453.409 kb on + strand at 453.412 kb on - strand at 453.419 kb on + strand at 453.436 kb on + strand at 453.441 kb on + strand at 453.441 kb on + strand at 453.444 kb on - strand at 453.446 kb on + strand at 453.449 kb on - strand at 453.449 kb on - strand at 453.449 kb on - strand at 453.449 kb on - strand at 453.449 kb on - strand at 453.449 kb on - strand at 453.449 kb on - strand at 453.454 kb on - strand at 453.477 kb on - strand at 453.479 kb on + strand at 453.479 kb on + strand at 453.492 kb on + strand at 453.501 kb on + strand at 453.531 kb on - strand at 453.545 kb on - strand at 453.553 kb on - strand at 453.579 kb on + strand at 453.591 kb on - strand at 453.593 kb on + strand at 453.596 kb on - strand at 453.596 kb on - strand at 453.596 kb on - strand at 453.601 kb on - strand, within GFF455 at 453.601 kb on - strand, within GFF455 at 453.603 kb on + strand, within GFF455 at 453.611 kb on - strand, within GFF455 at 453.632 kb on - strand, within GFF455 at 453.637 kb on + strand, within GFF455 at 453.637 kb on - strand, within GFF455 at 453.642 kb on - strand, within GFF455 at 453.645 kb on - strand, within GFF455 at 453.647 kb on - strand, within GFF455 at 453.680 kb on - strand, within GFF455 at 453.698 kb on - strand, within GFF455 at 453.703 kb on - strand, within GFF455 at 453.761 kb on + strand, within GFF455 at 453.770 kb on - strand, within GFF455 at 453.780 kb on + strand, within GFF455 at 453.788 kb on - strand, within GFF455 at 453.842 kb on + strand, within GFF455 at 453.848 kb on + strand, within GFF455 at 453.850 kb on - strand, within GFF455 at 453.867 kb on + strand at 453.873 kb on - strand at 453.878 kb on + strand at 453.886 kb on - strand at 453.886 kb on - strand at 453.896 kb on + strand at 453.910 kb on + strand at 453.941 kb on - strand at 453.945 kb on + strand at 453.945 kb on + strand at 453.950 kb on + strand at 453.963 kb on - strand at 453.967 kb on + strand at 454.034 kb on - strand, within GFF456 at 454.041 kb on + strand, within GFF456 at 454.046 kb on + strand, within GFF456 at 454.049 kb on - strand, within GFF456 at 454.049 kb on - strand, within GFF456 at 454.049 kb on - strand, within GFF456 at 454.054 kb on - strand, within GFF456 at 454.057 kb on - strand, within GFF456 at 454.067 kb on + strand, within GFF456 at 454.083 kb on + strand, within GFF456 at 454.093 kb on - strand, within GFF456 at 454.104 kb on + strand, within GFF456 at 454.119 kb on + strand, within GFF456 at 454.129 kb on + strand, within GFF456 at 454.137 kb on - strand, within GFF456 at 454.137 kb on - strand, within GFF456 at 454.172 kb on - strand, within GFF456 at 454.341 kb on + strand, within GFF456 at 454.346 kb on + strand, within GFF456 at 454.346 kb on + strand, within GFF456 at 454.346 kb on + strand, within GFF456 at 454.349 kb on - strand, within GFF456 at 454.349 kb on - strand, within GFF456 at 454.349 kb on - strand, within GFF456 at 454.354 kb on - strand, within GFF456 at 454.354 kb on - strand, within GFF456 at 454.354 kb on - strand, within GFF456 at 454.354 kb on - strand, within GFF456 at 454.354 kb on - strand, within GFF456 at 454.354 kb on - strand, within GFF456 at 454.398 kb on + strand, within GFF456 at 454.406 kb on - strand, within GFF456 at 454.419 kb on - strand, within GFF456 at 454.422 kb on + strand, within GFF456 at 454.468 kb on - strand, within GFF456 at 454.482 kb on + strand, within GFF456 at 454.487 kb on + strand, within GFF456 at 454.487 kb on + strand, within GFF456 at 454.490 kb on - strand, within GFF456 at 454.490 kb on - strand, within GFF456 at 454.490 kb on - strand, within GFF456 at 454.495 kb on - strand, within GFF456 at 454.497 kb on - strand, within GFF456 at 454.533 kb on + strand, within GFF456 at 454.533 kb on + strand, within GFF456 at 454.533 kb on + strand, within GFF456 at 454.533 kb on - strand, within GFF456 at 454.541 kb on - strand, within GFF456 at 454.541 kb on - strand, within GFF456 at 454.541 kb on - strand, within GFF456 at 454.541 kb on - strand, within GFF456 at 454.551 kb on + strand, within GFF456 at 454.559 kb on - strand, within GFF456 at 454.559 kb on - strand, within GFF456 at 454.561 kb on + strand, within GFF456 at 454.569 kb on - strand, within GFF456 at 454.572 kb on + strand, within GFF456 at 454.588 kb on - strand, within GFF456 at 454.588 kb on - strand, within GFF456 at 454.605 kb on - strand, within GFF456 at 454.614 kb on + strand, within GFF456 at 454.633 kb on + strand, within GFF456 at 454.641 kb on - strand, within GFF456 at 454.653 kb on + strand, within GFF456 at 454.653 kb on + strand, within GFF456 at 454.656 kb on - strand, within GFF456 at 454.658 kb on + strand, within GFF456 at 454.658 kb on + strand, within GFF456 at 454.661 kb on - strand, within GFF456 at 454.661 kb on - strand, within GFF456 at 454.661 kb on - strand, within GFF456 at 454.661 kb on - strand, within GFF456 at 454.663 kb on + strand, within GFF456 at 454.666 kb on - strand, within GFF456 at 454.690 kb on + strand, within GFF456 at 454.821 kb on - strand at 454.832 kb on - strand at 454.844 kb on + strand at 454.861 kb on + strand at 454.863 kb on + strand at 454.868 kb on + strand at 454.871 kb on - strand at 454.871 kb on - strand at 454.871 kb on - strand at 454.872 kb on + strand at 454.873 kb on + strand at 454.880 kb on - strand at 454.880 kb on - strand at 454.880 kb on - strand at 454.880 kb on - strand at 454.884 kb on + strand at 454.889 kb on + strand at 454.889 kb on + strand at 454.892 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Ammonium Sulfate remove 452,567 + GFF453 0.77 +0.7 452,575 - GFF453 0.77 +0.5 452,575 - GFF453 0.77 -0.3 452,610 - GFF453 0.79 -0.3 452,640 - GFF453 0.81 -0.7 452,640 - GFF453 0.81 +0.7 452,659 + GFF453 0.82 -0.0 452,659 + GFF453 0.82 +1.1 452,659 + GFF453 0.82 -1.2 452,664 + GFF453 0.82 +0.9 452,666 + GFF453 0.82 -1.5 452,667 - GFF453 0.83 +0.3 452,667 - GFF453 0.83 -1.8 452,672 - GFF453 0.83 +0.6 452,672 - GFF453 0.83 -0.5 452,674 - GFF453 0.83 -0.3 452,684 - GFF453 0.84 +0.8 452,692 - GFF453 0.84 +0.4 452,699 + GFF453 0.84 -0.9 452,748 + GFF453 0.87 -0.1 452,757 + GFF453 0.88 -0.4 452,776 + GFF453 0.89 -0.3 452,798 + -1.8 452,801 - +0.5 452,801 - -0.6 452,806 - -0.7 452,806 - -1.5 452,806 - -0.2 452,806 - +0.6 452,820 - -1.2 452,824 - +0.4 452,833 - +0.4 452,871 + +0.9 452,876 + -2.3 452,881 + -0.3 452,885 + +1.0 452,885 + -1.3 452,891 + +0.5 452,893 - +0.3 452,893 - -0.2 452,893 - -1.5 452,894 - +2.3 452,894 - +0.3 452,904 - -0.7 452,947 + +0.5 452,948 + -1.0 452,948 + -0.3 452,948 + +0.4 452,948 + -0.2 452,956 - +1.3 452,980 - -0.4 452,982 + -1.9 452,982 + +0.9 453,029 + GFF454 0.14 +2.4 453,029 + GFF454 0.14 +0.5 453,055 + GFF454 0.19 -0.4 453,055 + GFF454 0.19 -1.7 453,055 + GFF454 0.19 +0.8 453,055 + GFF454 0.19 +0.8 453,056 + GFF454 0.19 +0.1 453,063 - GFF454 0.21 -0.2 453,063 - GFF454 0.21 -0.7 453,080 - GFF454 0.25 -0.2 453,094 - GFF454 0.28 -0.8 453,099 - GFF454 0.29 -0.4 453,099 - GFF454 0.29 +0.4 453,104 + GFF454 0.30 -0.7 453,107 - GFF454 0.30 -1.3 453,112 - GFF454 0.31 +1.0 453,127 + GFF454 0.35 +0.0 453,128 + GFF454 0.35 +0.5 453,178 + GFF454 0.46 -0.0 453,186 - GFF454 0.47 +0.7 453,186 - GFF454 0.47 -2.9 453,186 - GFF454 0.47 +1.0 453,186 - GFF454 0.47 -0.7 453,203 + GFF454 0.51 +0.5 453,233 + GFF454 0.57 -0.8 453,233 + GFF454 0.57 -0.2 453,241 - GFF454 0.59 -0.8 453,242 - GFF454 0.59 -2.3 453,242 - GFF454 0.59 +1.0 453,251 + GFF454 0.61 +0.6 453,251 - GFF454 0.61 +0.2 453,251 - GFF454 0.61 -1.0 453,256 + GFF454 0.62 +0.8 453,259 - GFF454 0.63 +0.1 453,264 - GFF454 0.64 +0.3 453,287 - GFF454 0.69 -1.3 453,299 + GFF454 0.72 -1.3 453,301 + GFF454 0.72 -1.3 453,307 - GFF454 0.73 -1.0 453,307 - GFF454 0.73 -2.1 453,307 - GFF454 0.73 -0.3 453,309 + GFF454 0.74 +0.2 453,309 + GFF454 0.74 +1.4 453,309 + GFF454 0.74 -1.9 453,309 + GFF454 0.74 -1.5 453,309 + GFF454 0.74 -0.5 453,309 + GFF454 0.74 -0.2 453,309 + GFF454 0.74 -1.9 453,309 + GFF454 0.74 +0.3 453,309 + GFF454 0.74 +1.4 453,317 - GFF454 0.75 -1.4 453,317 - GFF454 0.75 +0.1 453,317 - GFF454 0.75 -1.1 453,317 - GFF454 0.75 -0.2 453,317 - GFF454 0.75 -1.8 453,317 - GFF454 0.75 -2.0 453,319 - GFF454 0.76 -0.9 453,319 - GFF454 0.76 -1.5 453,358 - GFF454 0.84 -1.2 453,409 + +0.9 453,412 - -1.0 453,419 + -1.8 453,436 + -0.2 453,441 + +1.0 453,441 + +0.6 453,444 - -0.4 453,446 + -0.2 453,449 - +0.5 453,449 - -0.6 453,449 - -1.0 453,449 - -0.7 453,449 - -0.7 453,449 - -1.2 453,449 - -1.1 453,454 - -0.1 453,477 - -1.1 453,479 + -0.9 453,479 + -0.4 453,492 + -0.7 453,501 + -2.1 453,531 - -1.2 453,545 - -0.2 453,553 - -0.7 453,579 + +0.7 453,591 - -0.2 453,593 + -0.0 453,596 - -0.2 453,596 - -1.4 453,596 - -0.7 453,601 - GFF455 0.10 -0.9 453,601 - GFF455 0.10 -1.1 453,603 + GFF455 0.11 -1.0 453,611 - GFF455 0.13 -0.8 453,632 - GFF455 0.20 -0.5 453,637 + GFF455 0.21 -0.5 453,637 - GFF455 0.21 -0.4 453,642 - GFF455 0.23 -1.2 453,645 - GFF455 0.24 -1.8 453,647 - GFF455 0.24 +0.4 453,680 - GFF455 0.34 +0.3 453,698 - GFF455 0.40 -1.0 453,703 - GFF455 0.41 +0.2 453,761 + GFF455 0.59 +0.9 453,770 - GFF455 0.62 -1.7 453,780 + GFF455 0.65 +0.3 453,788 - GFF455 0.67 -0.8 453,842 + GFF455 0.83 +0.3 453,848 + GFF455 0.85 -0.8 453,850 - GFF455 0.86 +0.2 453,867 + +1.1 453,873 - +0.9 453,878 + -2.2 453,886 - -0.7 453,886 - +0.1 453,896 + +0.3 453,910 + -0.7 453,941 - -0.9 453,945 + -0.2 453,945 + -0.2 453,950 + -0.3 453,963 - +0.6 453,967 + +1.1 454,034 - GFF456 0.15 -1.0 454,041 + GFF456 0.15 +0.4 454,046 + GFF456 0.16 -0.6 454,049 - GFF456 0.16 -1.3 454,049 - GFF456 0.16 +0.7 454,049 - GFF456 0.16 +0.5 454,054 - GFF456 0.17 -0.5 454,057 - GFF456 0.17 -2.1 454,067 + GFF456 0.18 +0.3 454,083 + GFF456 0.20 +0.5 454,093 - GFF456 0.21 -1.4 454,104 + GFF456 0.22 -1.1 454,119 + GFF456 0.23 -0.1 454,129 + GFF456 0.24 -1.6 454,137 - GFF456 0.25 -0.4 454,137 - GFF456 0.25 -1.4 454,172 - GFF456 0.29 -1.2 454,341 + GFF456 0.46 +0.8 454,346 + GFF456 0.47 -2.0 454,346 + GFF456 0.47 -1.2 454,346 + GFF456 0.47 -0.8 454,349 - GFF456 0.47 +1.0 454,349 - GFF456 0.47 +0.0 454,349 - GFF456 0.47 -1.3 454,354 - GFF456 0.48 -1.2 454,354 - GFF456 0.48 +0.5 454,354 - GFF456 0.48 +0.4 454,354 - GFF456 0.48 +0.4 454,354 - GFF456 0.48 -0.3 454,354 - GFF456 0.48 +1.1 454,398 + GFF456 0.52 -0.8 454,406 - GFF456 0.53 +0.5 454,419 - GFF456 0.54 -0.2 454,422 + GFF456 0.55 -0.8 454,468 - GFF456 0.59 -1.1 454,482 + GFF456 0.61 -1.0 454,487 + GFF456 0.61 -1.1 454,487 + GFF456 0.61 -0.6 454,490 - GFF456 0.62 -0.5 454,490 - GFF456 0.62 -0.3 454,490 - GFF456 0.62 +0.0 454,495 - GFF456 0.62 -0.7 454,497 - GFF456 0.62 +0.4 454,533 + GFF456 0.66 +0.5 454,533 + GFF456 0.66 -1.3 454,533 + GFF456 0.66 +0.1 454,533 - GFF456 0.66 +0.3 454,541 - GFF456 0.67 -1.1 454,541 - GFF456 0.67 -0.6 454,541 - GFF456 0.67 -0.5 454,541 - GFF456 0.67 -0.9 454,551 + GFF456 0.68 +0.6 454,559 - GFF456 0.69 -0.8 454,559 - GFF456 0.69 -0.8 454,561 + GFF456 0.69 +0.1 454,569 - GFF456 0.70 -0.9 454,572 + GFF456 0.70 -0.3 454,588 - GFF456 0.72 +1.4 454,588 - GFF456 0.72 +1.3 454,605 - GFF456 0.73 +0.8 454,614 + GFF456 0.74 -0.8 454,633 + GFF456 0.76 -0.8 454,641 - GFF456 0.77 -0.2 454,653 + GFF456 0.78 +0.1 454,653 + GFF456 0.78 -0.4 454,656 - GFF456 0.79 +0.2 454,658 + GFF456 0.79 -0.3 454,658 + GFF456 0.79 +1.7 454,661 - GFF456 0.79 +0.4 454,661 - GFF456 0.79 -0.5 454,661 - GFF456 0.79 -0.4 454,661 - GFF456 0.79 -0.4 454,663 + GFF456 0.79 -0.8 454,666 - GFF456 0.80 -0.8 454,690 + GFF456 0.82 -1.0 454,821 - -0.2 454,832 - +1.2 454,844 + +0.3 454,861 + -0.9 454,863 + -0.8 454,868 + -0.5 454,871 - -1.0 454,871 - +1.1 454,871 - -1.1 454,872 + -0.1 454,873 + -1.6 454,880 - +0.6 454,880 - -0.6 454,880 - +0.5 454,880 - -0.9 454,884 + -0.6 454,889 + +0.1 454,889 + -0.3 454,892 - -1.2
Or see this region's nucleotide sequence