Experiment: EMM_noCarbon with Sucrose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2221 and GFF2222 are separated by 98 nucleotides GFF2222 and GFF2223 overlap by 4 nucleotides GFF2223 and GFF2224 are separated by 76 nucleotides
GFF2221 - Proline dehydrogenase (EC 1.5.5.2) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.2.1.88), at 2,237,944 to 2,241,570
GFF2221
GFF2222 - PutR, transcriptional activator of PutA and PutP, at 2,241,669 to 2,242,154
GFF2222
GFF2223 - hypothetical protein, at 2,242,151 to 2,242,354
GFF2223
GFF2224 - putative secreted protein, at 2,242,431 to 2,243,660
GFF2224
Position (kb)
2241
2242
2243 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2240.669 kb on - strand, within GFF2221 at 2240.782 kb on + strand, within GFF2221 at 2240.786 kb on - strand, within GFF2221 at 2240.786 kb on - strand, within GFF2221 at 2240.857 kb on - strand, within GFF2221 at 2240.891 kb on + strand, within GFF2221 at 2240.891 kb on + strand, within GFF2221 at 2240.891 kb on + strand, within GFF2221 at 2240.940 kb on + strand, within GFF2221 at 2240.948 kb on - strand, within GFF2221 at 2240.976 kb on - strand, within GFF2221 at 2241.056 kb on - strand, within GFF2221 at 2241.123 kb on + strand, within GFF2221 at 2241.131 kb on + strand, within GFF2221 at 2241.131 kb on + strand, within GFF2221 at 2241.157 kb on - strand, within GFF2221 at 2241.199 kb on + strand, within GFF2221 at 2241.204 kb on + strand, within GFF2221 at 2241.282 kb on - strand at 2241.313 kb on + strand at 2241.313 kb on + strand at 2241.313 kb on + strand at 2241.323 kb on - strand at 2241.323 kb on - strand at 2241.391 kb on - strand at 2241.404 kb on + strand at 2241.428 kb on + strand at 2241.441 kb on - strand at 2241.441 kb on - strand at 2241.546 kb on - strand at 2241.562 kb on - strand at 2241.573 kb on + strand at 2241.582 kb on - strand at 2241.731 kb on + strand, within GFF2222 at 2241.731 kb on + strand, within GFF2222 at 2241.731 kb on + strand, within GFF2222 at 2241.731 kb on + strand, within GFF2222 at 2241.731 kb on + strand, within GFF2222 at 2241.731 kb on + strand, within GFF2222 at 2241.731 kb on + strand, within GFF2222 at 2241.731 kb on + strand, within GFF2222 at 2241.731 kb on + strand, within GFF2222 at 2241.739 kb on - strand, within GFF2222 at 2241.739 kb on - strand, within GFF2222 at 2241.739 kb on - strand, within GFF2222 at 2241.739 kb on - strand, within GFF2222 at 2241.739 kb on - strand, within GFF2222 at 2241.739 kb on - strand, within GFF2222 at 2241.739 kb on - strand, within GFF2222 at 2241.739 kb on - strand, within GFF2222 at 2241.739 kb on - strand, within GFF2222 at 2241.739 kb on - strand, within GFF2222 at 2241.750 kb on + strand, within GFF2222 at 2241.750 kb on + strand, within GFF2222 at 2241.750 kb on + strand, within GFF2222 at 2241.758 kb on - strand, within GFF2222 at 2241.772 kb on + strand, within GFF2222 at 2241.772 kb on + strand, within GFF2222 at 2241.780 kb on - strand, within GFF2222 at 2241.780 kb on - strand, within GFF2222 at 2241.780 kb on - strand, within GFF2222 at 2241.780 kb on - strand, within GFF2222 at 2241.782 kb on + strand, within GFF2222 at 2241.782 kb on + strand, within GFF2222 at 2241.782 kb on + strand, within GFF2222 at 2241.782 kb on + strand, within GFF2222 at 2241.782 kb on + strand, within GFF2222 at 2241.782 kb on + strand, within GFF2222 at 2241.782 kb on + strand, within GFF2222 at 2241.782 kb on + strand, within GFF2222 at 2241.782 kb on + strand, within GFF2222 at 2241.790 kb on - strand, within GFF2222 at 2241.790 kb on - strand, within GFF2222 at 2241.790 kb on - strand, within GFF2222 at 2241.790 kb on - strand, within GFF2222 at 2241.790 kb on - strand, within GFF2222 at 2241.790 kb on - strand, within GFF2222 at 2241.790 kb on - strand, within GFF2222 at 2241.790 kb on - strand, within GFF2222 at 2241.790 kb on - strand, within GFF2222 at 2241.790 kb on - strand, within GFF2222 at 2241.790 kb on - strand, within GFF2222 at 2241.790 kb on - strand, within GFF2222 at 2241.790 kb on - strand, within GFF2222 at 2241.790 kb on - strand, within GFF2222 at 2241.824 kb on - strand, within GFF2222 at 2241.869 kb on + strand, within GFF2222 at 2241.869 kb on + strand, within GFF2222 at 2241.869 kb on + strand, within GFF2222 at 2241.898 kb on + strand, within GFF2222 at 2241.898 kb on + strand, within GFF2222 at 2241.905 kb on + strand, within GFF2222 at 2241.913 kb on - strand, within GFF2222 at 2241.920 kb on + strand, within GFF2222 at 2241.969 kb on - strand, within GFF2222 at 2242.042 kb on + strand, within GFF2222 at 2242.066 kb on + strand, within GFF2222 at 2242.074 kb on - strand, within GFF2222 at 2242.076 kb on + strand, within GFF2222 at 2242.076 kb on + strand, within GFF2222 at 2242.084 kb on - strand, within GFF2222 at 2242.084 kb on - strand, within GFF2222 at 2242.084 kb on - strand, within GFF2222 at 2242.084 kb on - strand, within GFF2222 at 2242.107 kb on + strand at 2242.127 kb on - strand at 2242.185 kb on - strand, within GFF2223 at 2242.200 kb on - strand, within GFF2223 at 2242.200 kb on - strand, within GFF2223 at 2242.200 kb on - strand, within GFF2223 at 2242.200 kb on - strand, within GFF2223 at 2242.205 kb on - strand, within GFF2223 at 2242.212 kb on + strand, within GFF2223 at 2242.255 kb on - strand, within GFF2223 at 2242.270 kb on + strand, within GFF2223 at 2242.293 kb on + strand, within GFF2223 at 2242.303 kb on - strand, within GFF2223 at 2242.308 kb on - strand, within GFF2223 at 2242.311 kb on + strand, within GFF2223 at 2242.313 kb on - strand, within GFF2223 at 2242.319 kb on - strand, within GFF2223 at 2242.319 kb on - strand, within GFF2223 at 2242.324 kb on - strand, within GFF2223 at 2242.324 kb on - strand, within GFF2223 at 2242.356 kb on + strand at 2242.365 kb on + strand at 2242.420 kb on + strand at 2242.420 kb on + strand at 2242.426 kb on - strand at 2242.426 kb on - strand at 2242.428 kb on - strand at 2242.428 kb on - strand at 2242.464 kb on - strand at 2242.488 kb on + strand at 2242.509 kb on + strand at 2242.517 kb on - strand at 2242.527 kb on - strand at 2242.551 kb on - strand at 2242.605 kb on + strand, within GFF2224 at 2242.605 kb on + strand, within GFF2224 at 2242.613 kb on - strand, within GFF2224 at 2242.627 kb on + strand, within GFF2224 at 2242.627 kb on + strand, within GFF2224 at 2242.635 kb on - strand, within GFF2224 at 2242.639 kb on - strand, within GFF2224 at 2242.658 kb on - strand, within GFF2224 at 2242.664 kb on + strand, within GFF2224 at 2242.671 kb on + strand, within GFF2224 at 2242.671 kb on + strand, within GFF2224 at 2242.673 kb on + strand, within GFF2224 at 2242.679 kb on - strand, within GFF2224 at 2242.679 kb on - strand, within GFF2224 at 2242.679 kb on - strand, within GFF2224 at 2242.679 kb on - strand, within GFF2224 at 2242.679 kb on - strand, within GFF2224 at 2242.694 kb on + strand, within GFF2224 at 2242.695 kb on - strand, within GFF2224 at 2242.702 kb on - strand, within GFF2224 at 2242.702 kb on - strand, within GFF2224 at 2242.702 kb on - strand, within GFF2224 at 2242.706 kb on - strand, within GFF2224 at 2242.709 kb on + strand, within GFF2224 at 2242.752 kb on + strand, within GFF2224 at 2242.756 kb on + strand, within GFF2224 at 2242.756 kb on + strand, within GFF2224 at 2242.756 kb on + strand, within GFF2224 at 2242.777 kb on + strand, within GFF2224 at 2242.782 kb on + strand, within GFF2224 at 2242.785 kb on - strand, within GFF2224 at 2242.785 kb on - strand, within GFF2224 at 2242.814 kb on + strand, within GFF2224 at 2242.822 kb on + strand, within GFF2224 at 2242.832 kb on + strand, within GFF2224 at 2242.840 kb on - strand, within GFF2224 at 2242.840 kb on - strand, within GFF2224 at 2242.840 kb on - strand, within GFF2224 at 2242.854 kb on - strand, within GFF2224 at 2242.857 kb on + strand, within GFF2224 at 2242.857 kb on + strand, within GFF2224 at 2242.857 kb on + strand, within GFF2224 at 2242.857 kb on + strand, within GFF2224 at 2242.857 kb on + strand, within GFF2224 at 2242.865 kb on + strand, within GFF2224 at 2242.865 kb on - strand, within GFF2224 at 2242.865 kb on - strand, within GFF2224 at 2242.865 kb on - strand, within GFF2224 at 2242.865 kb on - strand, within GFF2224 at 2242.865 kb on - strand, within GFF2224 at 2242.865 kb on - strand, within GFF2224 at 2242.865 kb on - strand, within GFF2224 at 2242.865 kb on - strand, within GFF2224 at 2242.865 kb on - strand, within GFF2224 at 2242.865 kb on - strand, within GFF2224 at 2242.865 kb on - strand, within GFF2224 at 2242.865 kb on - strand, within GFF2224 at 2242.876 kb on + strand, within GFF2224 at 2242.881 kb on + strand, within GFF2224 at 2242.884 kb on - strand, within GFF2224 at 2242.884 kb on - strand, within GFF2224 at 2242.884 kb on - strand, within GFF2224 at 2242.884 kb on - strand, within GFF2224 at 2242.884 kb on - strand, within GFF2224 at 2242.889 kb on + strand, within GFF2224 at 2242.895 kb on + strand, within GFF2224 at 2242.920 kb on + strand, within GFF2224 at 2242.932 kb on + strand, within GFF2224 at 2242.993 kb on - strand, within GFF2224 at 2242.999 kb on + strand, within GFF2224 at 2243.007 kb on - strand, within GFF2224 at 2243.008 kb on + strand, within GFF2224 at 2243.022 kb on + strand, within GFF2224 at 2243.041 kb on + strand, within GFF2224 at 2243.067 kb on + strand, within GFF2224 at 2243.085 kb on + strand, within GFF2224 at 2243.085 kb on + strand, within GFF2224 at 2243.093 kb on - strand, within GFF2224 at 2243.093 kb on - strand, within GFF2224 at 2243.093 kb on - strand, within GFF2224
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Ammonium Sulfate remove 2,240,669 - GFF2221 0.75 +0.4 2,240,782 + GFF2221 0.78 +0.2 2,240,786 - GFF2221 0.78 +0.1 2,240,786 - GFF2221 0.78 +0.4 2,240,857 - GFF2221 0.80 -2.9 2,240,891 + GFF2221 0.81 +0.3 2,240,891 + GFF2221 0.81 +1.2 2,240,891 + GFF2221 0.81 -1.5 2,240,940 + GFF2221 0.83 -1.1 2,240,948 - GFF2221 0.83 -0.6 2,240,976 - GFF2221 0.84 -0.3 2,241,056 - GFF2221 0.86 +1.0 2,241,123 + GFF2221 0.88 -1.0 2,241,131 + GFF2221 0.88 -0.8 2,241,131 + GFF2221 0.88 +0.6 2,241,157 - GFF2221 0.89 -0.7 2,241,199 + GFF2221 0.90 +0.6 2,241,204 + GFF2221 0.90 -0.2 2,241,282 - +2.0 2,241,313 + -0.3 2,241,313 + -0.8 2,241,313 + +0.2 2,241,323 - +0.1 2,241,323 - +0.4 2,241,391 - -1.5 2,241,404 + -1.2 2,241,428 + -0.3 2,241,441 - -0.3 2,241,441 - -2.0 2,241,546 - -1.7 2,241,562 - -1.2 2,241,573 + -0.7 2,241,582 - +0.1 2,241,731 + GFF2222 0.13 -1.2 2,241,731 + GFF2222 0.13 -1.4 2,241,731 + GFF2222 0.13 -1.6 2,241,731 + GFF2222 0.13 +0.3 2,241,731 + GFF2222 0.13 -2.6 2,241,731 + GFF2222 0.13 -1.5 2,241,731 + GFF2222 0.13 -1.3 2,241,731 + GFF2222 0.13 -0.5 2,241,731 + GFF2222 0.13 -0.7 2,241,739 - GFF2222 0.14 +1.2 2,241,739 - GFF2222 0.14 +0.2 2,241,739 - GFF2222 0.14 -1.3 2,241,739 - GFF2222 0.14 -0.4 2,241,739 - GFF2222 0.14 +0.7 2,241,739 - GFF2222 0.14 -1.7 2,241,739 - GFF2222 0.14 +0.3 2,241,739 - GFF2222 0.14 +0.1 2,241,739 - GFF2222 0.14 -0.2 2,241,739 - GFF2222 0.14 -0.4 2,241,750 + GFF2222 0.17 +0.1 2,241,750 + GFF2222 0.17 -0.3 2,241,750 + GFF2222 0.17 -1.3 2,241,758 - GFF2222 0.18 -2.9 2,241,772 + GFF2222 0.21 -1.7 2,241,772 + GFF2222 0.21 +0.3 2,241,780 - GFF2222 0.23 -0.4 2,241,780 - GFF2222 0.23 +0.1 2,241,780 - GFF2222 0.23 -1.0 2,241,780 - GFF2222 0.23 +0.4 2,241,782 + GFF2222 0.23 -1.0 2,241,782 + GFF2222 0.23 -1.9 2,241,782 + GFF2222 0.23 -0.3 2,241,782 + GFF2222 0.23 -1.4 2,241,782 + GFF2222 0.23 -1.2 2,241,782 + GFF2222 0.23 -2.0 2,241,782 + GFF2222 0.23 -1.0 2,241,782 + GFF2222 0.23 -1.4 2,241,782 + GFF2222 0.23 -1.5 2,241,790 - GFF2222 0.25 +0.9 2,241,790 - GFF2222 0.25 -1.2 2,241,790 - GFF2222 0.25 +1.0 2,241,790 - GFF2222 0.25 +1.1 2,241,790 - GFF2222 0.25 +0.3 2,241,790 - GFF2222 0.25 +0.5 2,241,790 - GFF2222 0.25 -0.4 2,241,790 - GFF2222 0.25 -1.5 2,241,790 - GFF2222 0.25 -0.2 2,241,790 - GFF2222 0.25 -1.8 2,241,790 - GFF2222 0.25 -0.8 2,241,790 - GFF2222 0.25 +0.7 2,241,790 - GFF2222 0.25 -0.8 2,241,790 - GFF2222 0.25 +0.9 2,241,824 - GFF2222 0.32 -1.5 2,241,869 + GFF2222 0.41 -0.9 2,241,869 + GFF2222 0.41 -1.3 2,241,869 + GFF2222 0.41 -2.2 2,241,898 + GFF2222 0.47 +0.7 2,241,898 + GFF2222 0.47 +1.1 2,241,905 + GFF2222 0.49 -1.5 2,241,913 - GFF2222 0.50 +0.4 2,241,920 + GFF2222 0.52 +0.8 2,241,969 - GFF2222 0.62 +1.0 2,242,042 + GFF2222 0.77 +0.2 2,242,066 + GFF2222 0.82 -0.0 2,242,074 - GFF2222 0.83 +0.4 2,242,076 + GFF2222 0.84 +0.8 2,242,076 + GFF2222 0.84 -0.0 2,242,084 - GFF2222 0.85 -0.3 2,242,084 - GFF2222 0.85 -0.5 2,242,084 - GFF2222 0.85 -0.5 2,242,084 - GFF2222 0.85 -0.1 2,242,107 + -0.7 2,242,127 - +0.2 2,242,185 - GFF2223 0.17 -0.6 2,242,200 - GFF2223 0.24 +0.2 2,242,200 - GFF2223 0.24 -0.5 2,242,200 - GFF2223 0.24 -0.4 2,242,200 - GFF2223 0.24 -0.1 2,242,205 - GFF2223 0.26 +0.5 2,242,212 + GFF2223 0.30 +0.5 2,242,255 - GFF2223 0.51 -0.4 2,242,270 + GFF2223 0.58 -0.6 2,242,293 + GFF2223 0.70 +0.6 2,242,303 - GFF2223 0.75 +0.6 2,242,308 - GFF2223 0.77 -0.0 2,242,311 + GFF2223 0.78 -0.1 2,242,313 - GFF2223 0.79 -0.7 2,242,319 - GFF2223 0.82 -0.9 2,242,319 - GFF2223 0.82 +0.7 2,242,324 - GFF2223 0.85 -1.6 2,242,324 - GFF2223 0.85 +0.0 2,242,356 + -1.8 2,242,365 + -0.8 2,242,420 + -0.6 2,242,420 + -0.1 2,242,426 - -0.4 2,242,426 - -2.7 2,242,428 - -0.6 2,242,428 - -0.1 2,242,464 - -0.9 2,242,488 + -0.2 2,242,509 + +0.7 2,242,517 - +0.8 2,242,527 - -1.5 2,242,551 - -1.2 2,242,605 + GFF2224 0.14 -0.4 2,242,605 + GFF2224 0.14 -1.3 2,242,613 - GFF2224 0.15 -0.6 2,242,627 + GFF2224 0.16 +1.7 2,242,627 + GFF2224 0.16 -0.5 2,242,635 - GFF2224 0.17 +0.3 2,242,639 - GFF2224 0.17 +0.6 2,242,658 - GFF2224 0.18 -1.2 2,242,664 + GFF2224 0.19 -1.6 2,242,671 + GFF2224 0.20 +0.5 2,242,671 + GFF2224 0.20 -0.6 2,242,673 + GFF2224 0.20 -1.0 2,242,679 - GFF2224 0.20 -0.4 2,242,679 - GFF2224 0.20 -0.5 2,242,679 - GFF2224 0.20 -0.5 2,242,679 - GFF2224 0.20 +2.0 2,242,679 - GFF2224 0.20 +0.3 2,242,694 + GFF2224 0.21 +1.9 2,242,695 - GFF2224 0.21 -1.1 2,242,702 - GFF2224 0.22 -0.0 2,242,702 - GFF2224 0.22 -1.0 2,242,702 - GFF2224 0.22 +0.4 2,242,706 - GFF2224 0.22 -0.6 2,242,709 + GFF2224 0.23 -2.2 2,242,752 + GFF2224 0.26 +0.9 2,242,756 + GFF2224 0.26 -1.6 2,242,756 + GFF2224 0.26 +0.7 2,242,756 + GFF2224 0.26 -0.5 2,242,777 + GFF2224 0.28 +0.1 2,242,782 + GFF2224 0.29 -0.6 2,242,785 - GFF2224 0.29 +0.4 2,242,785 - GFF2224 0.29 -1.3 2,242,814 + GFF2224 0.31 +0.0 2,242,822 + GFF2224 0.32 +0.1 2,242,832 + GFF2224 0.33 +1.3 2,242,840 - GFF2224 0.33 +0.2 2,242,840 - GFF2224 0.33 +0.2 2,242,840 - GFF2224 0.33 +0.8 2,242,854 - GFF2224 0.34 -2.7 2,242,857 + GFF2224 0.35 -0.6 2,242,857 + GFF2224 0.35 -0.5 2,242,857 + GFF2224 0.35 -0.0 2,242,857 + GFF2224 0.35 +0.0 2,242,857 + GFF2224 0.35 +0.8 2,242,865 + GFF2224 0.35 +0.4 2,242,865 - GFF2224 0.35 +0.4 2,242,865 - GFF2224 0.35 +0.8 2,242,865 - GFF2224 0.35 -0.3 2,242,865 - GFF2224 0.35 +0.6 2,242,865 - GFF2224 0.35 +0.6 2,242,865 - GFF2224 0.35 -1.4 2,242,865 - GFF2224 0.35 -1.2 2,242,865 - GFF2224 0.35 +0.6 2,242,865 - GFF2224 0.35 -1.6 2,242,865 - GFF2224 0.35 +0.2 2,242,865 - GFF2224 0.35 -0.2 2,242,865 - GFF2224 0.35 -0.4 2,242,876 + GFF2224 0.36 -1.8 2,242,881 + GFF2224 0.37 +0.4 2,242,884 - GFF2224 0.37 -0.6 2,242,884 - GFF2224 0.37 -0.1 2,242,884 - GFF2224 0.37 -0.3 2,242,884 - GFF2224 0.37 +0.8 2,242,884 - GFF2224 0.37 -1.4 2,242,889 + GFF2224 0.37 -0.6 2,242,895 + GFF2224 0.38 -0.6 2,242,920 + GFF2224 0.40 -1.7 2,242,932 + GFF2224 0.41 -2.2 2,242,993 - GFF2224 0.46 +0.3 2,242,999 + GFF2224 0.46 -0.8 2,243,007 - GFF2224 0.47 -0.2 2,243,008 + GFF2224 0.47 -1.6 2,243,022 + GFF2224 0.48 -0.7 2,243,041 + GFF2224 0.50 +1.0 2,243,067 + GFF2224 0.52 -1.9 2,243,085 + GFF2224 0.53 -0.7 2,243,085 + GFF2224 0.53 -0.4 2,243,093 - GFF2224 0.54 -0.6 2,243,093 - GFF2224 0.54 -0.5 2,243,093 - GFF2224 0.54 -0.0
Or see this region's nucleotide sequence