Experiment: EMM_noCarbon with Sucrose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF177 and GFF178 are separated by 86 nucleotides GFF178 and GFF179 overlap by 23 nucleotides
GFF177 - LPS-assembly protein LptD @ Organic solvent tolerance protein precursor, at 178,885 to 181,149
GFF177
GFF178 - Periplasmic chaperone and peptidyl-prolyl cis-trans isomerase of outer membrane proteins SurA (EC 5.2.1.8), at 181,236 to 182,600
GFF178
GFF179 - 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262), at 182,578 to 183,603
GFF179
GFF180 - SSU rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase (EC 2.1.1.182), at 183,593 to 184,447
GFF180
Position (kb) 181
182
183 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 181.136 kb on + strand at 181.146 kb on + strand at 181.164 kb on - strand at 181.171 kb on + strand at 181.171 kb on + strand at 181.171 kb on + strand at 181.176 kb on + strand at 181.179 kb on - strand at 181.181 kb on + strand at 181.189 kb on - strand at 181.196 kb on + strand at 181.212 kb on - strand at 181.213 kb on - strand at 183.591 kb on - strand at 183.591 kb on - strand at 183.599 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Ammonium Sulfate remove 181,136 + -0.2 181,146 + +0.1 181,164 - -1.0 181,171 + -3.0 181,171 + -1.2 181,171 + -2.5 181,176 + +0.1 181,179 - +1.3 181,181 + +0.3 181,189 - -0.3 181,196 + -1.1 181,212 - +1.0 181,213 - +1.6 183,591 - +0.7 183,591 - +0.1 183,599 + -1.6
Or see this region's nucleotide sequence