Strain Fitness in Escherichia coli ECRC100 around OKFHMN_28570

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntTyr_trna and Tyr_trna are separated by 209 nucleotidesTyr_trna and purU are separated by 159 nucleotidespurU and ychJ are separated by 49 nucleotidesychJ and rssA are separated by 73 nucleotides OKFHMN_28560: Tyr_trna - tRNA-Tyr(gta), at 5,480,906 to 5,480,990 _trna OKFHMN_28565: Tyr_trna - tRNA-Tyr(gta), at 5,481,200 to 5,481,284 _trna OKFHMN_28570: purU - formyltetrahydrofolate deformylase, at 5,481,444 to 5,482,286 purU OKFHMN_28575: ychJ - YchJ family protein, at 5,482,336 to 5,482,794 ychJ OKFHMN_28580: rssA - patatin-like phospholipase RssA, at 5,482,868 to 5,483,812 rssA Position (kb) 5481 5482 5483Strain fitness (log2 ratio) -1 0 1 2at 5481.014 kb on - strandat 5481.044 kb on - strandat 5481.120 kb on - strandat 5481.552 kb on - strand, within purUat 5481.670 kb on + strand, within purUat 5481.915 kb on + strand, within purUat 5482.253 kb on + strandat 5482.425 kb on - strand, within ychJat 5482.648 kb on + strand, within ychJat 5482.653 kb on - strand, within ychJat 5482.737 kb on - strand, within ychJat 5482.737 kb on - strand, within ychJat 5482.750 kb on + strandat 5482.755 kb on + strandat 5483.193 kb on + strand, within rssA

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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5,481,014 - +0.7
5,481,044 - -0.3
5,481,120 - +0.6
5,481,552 - purU OKFHMN_28570 0.13 +2.2
5,481,670 + purU OKFHMN_28570 0.27 -0.1
5,481,915 + purU OKFHMN_28570 0.56 -0.2
5,482,253 + +0.9
5,482,425 - ychJ OKFHMN_28575 0.19 +0.5
5,482,648 + ychJ OKFHMN_28575 0.68 -0.2
5,482,653 - ychJ OKFHMN_28575 0.69 +1.3
5,482,737 - ychJ OKFHMN_28575 0.87 -0.1
5,482,737 - ychJ OKFHMN_28575 0.87 -0.1
5,482,750 + +0.9
5,482,755 + -1.0
5,483,193 + rssA OKFHMN_28580 0.34 -0.1

Or see this region's nucleotide sequence